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1.
Stearine fish oil (SFO) and palm oil (PO) have emerged as promising alternatives for the replacement of fish oil (FO) in aquafeeds. This study evaluated the replacement of FO with alternative oils in practical diets for Litopenaeus vannamei. In a clear brackish water study (14.1 g/L) utilizing shrimp (0.29 ± 0.02 g, initial weight), FO was replaced by SFO at inclusion ratios of 100:0, 75:25, 50:50, 25:75, and 0:100 (FO:SFO) and PO as 90% of FO. After 55 days, no significant differences (p < 0.05) in final weight, growth, or survival of shrimp were observed. A second trial (8 weeks) in low‐salinity water (2.1 g/L) with shrimp (0.92 ± 0.02 g, initial weight) evaluated diets with 100% FO, 100% SFO, 90% PO, 90% soybean oil (SO), or 90% flaxseed oil (FXO) as a replacement for FO and four commercially produced diets with 2% of FO, SO, PO, or FXO. One treatment received half rations of the commercial FO diet, and one treatment was based entirely on natural productivity. Results show that the fatty acid profiles of the tail muscle conformed to the lipids of the feed, and highly unsaturated fatty acids (HUFAs) were preserved. Following 8 weeks of culture, there were no significant differences in production performance.  相似文献   
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Cyclophosphamide (CP) is an alkylating agent commonly included in multi-drug treatment protocols for canine cancer. As a prodrug, CP requires hepatic metabolism for activation to the intermediate compound 4-hydroxycyclophosphamide (4-OHCP) which then spontaneously forms alkylating phosphoramide mustard. CP is frequently administered in a fractionated manner, with the total dose given over multiple days. CP is reported to cause auto-induction of metabolism in humans, with faster CP clearance and relatively increased 4-OHCP formation following fractionated versus bolus dosing, however canine pharmacokinetic studies of CP dose fractionation are lacking. The study objective was to evaluate the pharmacokinetics of fractionated oral CP dosing at a dose of 200–250 mg/m2 over 3 to 4 days in a prospectively identified population of cancer-bearing dogs. Plasma concentrations of CP and 4-OHCP were measured by ultra-high performance liquid chromatography tandem-mass spectrometry in eight dogs following the first and last doses to assess for auto-induction of CP metabolism. No significant difference in the rate of CP elimination between first and last doses were detected (0.73 ± 0.46 vs. 1.22 ± 0.5 h−1; p = .125). Additionally, no significant difference in dose-normalized 4-OHCP exposure was identified between first and last doses (5.9 ± 2.1 vs. 7.9 ± 6.4 h × ng/ml; p = .936). These results suggest that fractionated dosing may not increase exposure to the active metabolite of CP in dogs as it does in humans. As such, standard bolus dosing and fractionated dosing may be equivalent in terms of bio-activation of CP in dogs administered a dose of 200–250 mg/m2.  相似文献   
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The golden shiner, Notemigonus crysoleucas, is a major bait and forage fish species in the USA. Farmers produce millions of golden shiners annually that are distributed live, many across state boundaries. Native over much of the eastern and central USA, this species is often present but rarely abundant in natural systems, as fish density is apparently controlled by predation rather than by food resources. Aquaculture of the golden shiner is a century‐old practice and culture methods have evolved over time, resulting in today's farm‐raised, certified specific pathogen– and aquatic nuisance species–free baitfish. Federal and state agencies and universities have a long history of developing culture methods and promoting baitfish culture to replace the indiscriminate and wasteful harvest of fish from natural waters. Despite advances in culture methods and the advantages of a farm‐raised species produced under controlled and biosecure conditions, golden shiner farming is only marginally profitable and remaining farmers survive on farm equity and increased market share from others exiting the business. Ironically, increasing regulations and restrictions leading to a reduction in the supply of farm‐raised baitfish could drive anglers back to harvesting bait from natural waters, the very concern that initiated government support for baitfish culture a century ago.  相似文献   
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Three sources of soybean meal (SBM) were biochemically characterized and evaluated in diets for juvenile Litopenaeus vannamei. Three biological techniques were utilized to evaluate the nutritional quality of the meals including an outdoor growth trial as well as determination of in vivo apparent digestibility and in vitro digestibility including pepsin digestibility (0.0002%), pH Stat degree of hydrolysis, and immobilized digestive enzyme assay (IDEA). The growth trial was conducted over a 10‐wk period using soybean meal as the primary protein source with each meal being included at 54–58% of the diet based on an equal protein inclusion. At the conclusion of the growth trial there were no significant differences between the test diets indicating equivalent biological availability under practical conditions. The in vivo digestibility trial utilized chromic oxide as the inert marker and 70:30 replacement techniques resulted in typical results. Apparent protein digestibility (APD) and apparent energy digestibility (AED) of the diets ranged from 66.8 to 80.2% and 65.4 to 74.7%, respectively. Ingredient digestibility values for protein and energy ranged from 78.8 to 93.5% and 60.5 to 85.0%, respectively. One of the three SBM evaluated had numerically lower protein digestibility and significantly lower energy digestibility than the other two meals .  相似文献   
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Over 3 years, 32,444 age‐0 group Atlantic salmon (Salmo salar) parr tagged with passive integrated transponder tags throughout the River Frome catchment were assigned to one of three groups, nonmigrants, autumn migrants and spring smolts, depending on the detection and the timing of detection at downstream tag readers (situated 8.6 km above the tidal limit). We examined the effect of density at the time of tagging (n·m?2), distance upstream from the tidal limit (km), fish length (mm), Fulton condition index, habitat type (divided into two types, main river and carrier), days after 1 September that each fish was tagged and year (replication) on the proportions of fish in each of the migration groups. Distance upstream from tidal limit was strongly negatively related to the proportion of autumn migrants and positively related to the proportion of spring smolts. Nonmigrants had a lower average body size than migrants, although there were no differences in the sizes of autumn migrants and spring smolts in September prior to migration. Fish density had no effect on migration strategy. A lower proportion of fish migrated as autumn migrants from the smaller carrier habitats than the main river channel. There is some evidence that those parr destined to become autumn migrants underwent a lower mortality rate during September before tagging than those destined to become spring migrants indicating possible physiological or behavioural differences between these two groups of fish at that time. More research into the factors responsible for initiating the autumn migration is required.  相似文献   
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An experiment to examine the effect of streptobacillary arthritis on mouse populations was conducted in maturing sorghum crops in mouse-proofed pens. There were three treatment pens and three control pens in which mice with and without clinical signs of streptobacillary arthritis were introduced, respectively. A constant mouse density (533 mice ha−1) was introduced to the pens by releasing 12 mice in each pen. The sex ratio of introduced mice was also constant by releasing in each pen nine males and three females. Mean track index was lower in treatment pens than in control pens, indicating that streptobacillary arthritis reduced the activity of mice. However, this was not translated into a reduction in other population responses. Mean mouse density at harvest, percentage of female pregnant at harvest, yield loss caused by mice and a feeding index (oil card index) did not differ significantly between treatment and control groups. A plausible explanation for these findings was that mice with the clinical signs might be less active but were able to consume as much grains as mice without the clinical signs because food was in abundant supply.  相似文献   
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