牦牛源正呼肠孤病毒2型的检测和分离鉴定     

吕珽  陈虹吟  汤承  岳华 《畜牧兽医学报》2021,52(8):2361-2368

旨在调查川西北牦牛哺乳动物正呼肠孤病毒（MRV）的感染情况并分离病毒。采用RT-PCR方法，对采自川西北15个牧场的72份牦牛腹泻粪便样本和其中5个牧场的15份腹泻牦牛血清样本进行MRV检测，阳性样本进一步用分型PCR确定其血清型。结果显示，粪便样本中MRV检出率为20.83%（15/72），血清2型的比例为60%（9/15）；血清样本中MRV检出率为40%（6/15），血清2型的比例为83.33%（5/6）；未检测到其他血清型。成功地从腹泻粪便中分离到1株MRV血清2型毒株（TCID₅₀为4×10^-8.56·mL^-1），并获得长度为23 587 bp的分离株全基因组，该分离株与中国猪源毒株的遗传关系最近；与GenBank中所有的MRV S1基因相比，该分离株有4个独特的氨基酸突变。本研究从牦牛中检测到MRV，并分离到1株牛源MRV血清2型毒株，为进一步研究MRV血清2型生物学特性奠定了基础。  相似文献   

葡萄风信子物候期观察与观赏性状分析     

吕文涛  娄晓鸣  赵雨菲 《安徽农业科学》2021,49(14):50-53

对荷兰引进的10个葡萄风信子品种进行露地栽培,对其物候期及观赏性状进行观察与评价.结果表明,引进的10个葡萄风信子品种在苏州地区露地栽培均能正常开花;其中"大微笑""幻想创造""蓝魔法""触雪"等品种适宜在园林中推广与应用.  相似文献   

Impact of grassland contract policy on soil organic carbon losses from alpine grassland on the Qinghai–Tibetan Plateau          下载免费PDF全文

J. Cao  Y. Gong  E. T. Yeh  N. M. Holden  J. F. Adamowski  R. C. Deo  M. Liu  J. Zhou  J. Zhang  W. Zhang  S. Zhang  D. Sheng  S. Yang  X. Xu  M. Li  Q. Feng 《Soil Use and Management》2017,33(4):663-671

Carbon storage in the soils on the Qinghai–Tibetan Plateau plays a very important role in the global carbon budget. In the 1990s, a policy of contracting collective grasslands to smaller units was implemented, resulting in a change from the traditional collective grassland management to two new management patterns: a multi‐household management pattern (MMP: grassland shared by several households without enclosures) and a single‐household management pattern (SMP: grassland enclosed and used by only one household). In 2016, 50 MMP and 54 SMP winter pastures on the Qinghai–Tibetan Plateau were sampled to assess the differences in soil organic carbon (SOC) between the two management patterns. Results showed that average SOC was significantly greater under MMP than under SMP, with an estimated 0.41 Mg C/ha/yr lost due to SMP following the new grassland contract. Based on the government's grassland policy, four grassland utilization scenarios were developed for both summer and winter pastures. We found that if the grassland were managed under SMP, likely C losses ranged between 0.31 × 10⁷ and 6.15 × 10⁷ Mg C/yr across the Qinghai–Tibetan Plateau relative to MMP, which more closely resembles pre‐1990s grassland management. Previous estimates of C losses have only considered land use change (with cover change) and ignored the impacts driven by land management pattern changes (without cover change). The new data suggest that C losses from the Qinghai–Tibetan Plateau are greater than previously estimated, and therefore that the grassland contract policy should be reviewed and SMP households should be encouraged to reunite into the MMP. These findings have potential implications for land management strategies not only on the Qinghai–Tibetan Plateau but also other grazing regions globally where such practices may exist.  相似文献   

Still Only One Earth. Progress in the 40 years since the First UN Conference on the Environment. By R.E. Hester & R.M. Harrison (eds). Published by Royal Society of Chemistry,Cambridge, 2015. vii + 285 pp. £67.50. Hardback (ISBN 978‐1‐78262‐076‐1).          下载免费PDF全文

R. L. Hough 《Soil Use and Management》2017,33(1):176-176

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Atypical thymoma in a horse: Diagnostic approach and application of an alternative histological classification system     

T. Pudert  A. Weyrich  F. Geburek  C. Herden  M. Röcken 《Equine Veterinary Education》2021,33(11):e386-e392

Thymoma is a rare condition in horses. This case of a 24-year-old Warmblood gelding that was presented with oedema and signs of vascular congestion of the left forelimb caused by a thoracic mass is described. The diagnostic work-up included blood chemistry, cytology of pleural effusion, ultrasonography, radiography and the visualisation and collection of a tissue sample by thoracoscopy. Finally, post-mortem findings and histopathology revealed thymic epithelial neoplasia with histomorphologic features previously unreported in horses. The unique mixture of spindle-shaped and epithelioid tumour cells in combination with clear features of malignancy suggested the application of the Moran and Suster histological classification system for thymomas in humans (Moran and Suster, 2008, Curr. Treat. Options Oncol., 9, 288), which has not been reported in an equine case before and fits well to the present case.  相似文献   

Do cover crops change the lability of phosphorus in a clayey subtropical soil under different phosphate fertilizers?          下载免费PDF全文

A. P. B. Teles  M. Rodrigues  W. F. Bejarano Herrera  A. Soltangheisi  L. R. Sartor  P. J. A. Withers  P. S. Pavinato 《Soil Use and Management》2017,33(1):34-44

Plants have developed different mechanisms to absorb and solubilize phosphorus (P) in the soil, especially in environments with low P availability. This study evaluated the effects of different winter cover crops on soil P availability in a clayey subtropical (Hapludox) soil receiving soluble P fertilizer and a rock phosphate applied to the summer crop, under no‐tillage. The experiment was carried out over 3 yrs (2009–2011) with five different cover crop species: common vetch, fodder radish, ryegrass, black oat, white clover and fallow as control. The soil was sampled after the third year of cover crop cultivation and analysed for inorganic and organic P forms according to the well‐established Hedley fractionation procedure. Phosphate fertilizers promoted accumulation of both labile and nonlabile P pools in soil in the near surface layer, especially under rock phosphate. Fertilizer applications were not able to change P fractions in deeper layers, emphasizing that the Brazilian clayey soils are a sink of P from fertilizer and its mobility is almost nil. Although the cover crops recycled a great amount of P in tissue, in a short‐term evaluation (3 yrs) they only changed the content of moderately labile P in soil, indicating that long‐term studies are needed for more conclusive results.  相似文献   

The effect of feeding a novel multistrain yeast fraction on European seabass (Dicentrachus labrax) intestinal health and growth performance     

Mark D. Rawling  Nicola Pontefract  Ana Rodiles  Ilektra Anagnostara  Eric Leclercq  Marion Schiavone  Mathieu Castex  Daniel L. Merrifield 《Journal of the World Aquaculture Society》2019,50(6):1108-1122

Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass.  相似文献   

香蕉新品种‘南天红’     

杨兴玉  肖维强  许林兵  李华平  黄秉智  叶春海  陈彪  陈谷  吕庆芳  梁钾贤  吴元立  胡玲玉 《园艺学报》2020,47(Z2):2961-2962

香蕉新品种‘南天红’由‘南天黄’的自然突变选育而成。假茎平均高度2.59 m，幼树假茎外表面暗红色，抽蕾后转绿。雄花苞片外表面常为深红色，花苞卷曲，常不脱落；从种植到收获11 ~ 13个月，单株产量25 kg，品质好。中高抗香蕉枯萎病4号小种，采用配套的栽培技术可在所有香蕉种植区推广。  相似文献   

Comparison of outcomes following treatment of septic calcaneal bursitis by needle or bursoscopic lavage: A retrospective study of 29 horses     

M. J. S. Duggan  T. S. Mair 《Equine Veterinary Education》2021,33(3):135-142

Sepsis of the calcaneal bursae (CB) presents significant treatment challenges with limited clinical data available in the literature. The objective of this retrospective cohort study was to assess the clinical outcomes associated with CB lavage using either a through-and-through needle or bursoscopic technique. Clinical records of 29 horses treated for septic calcaneal bursitis using either technique between 2005 and 2019 were reviewed. Fisher’s exact test was used to assess statistical significance between first surgical technique and success at first surgery (i.e. not requiring >1 lavage), survival to discharge and return to work (RTW). Bursoscopy was performed in 13/29 (44.8%) cases, and needle lavage in 16/29 (55.2%). In the needle group, 12 (75%) horses were discharged following the first surgery. Four had repeat interventions; two (12.5%) had needle lavage and two (12.5%) had bursoscopy. Of the two horses to have repeat needle lavage, one was subjected to euthanasia and one discharged, and of the two that underwent bursoscopy, one was discharged and one received a third bursoscopy prior to discharge. In the bursoscopy group, seven (53.8%) were discharged and three (23.1%) were subjected to euthanasia following the first surgery. Three (23.1%) received a second bursoscopic lavage with one discharged, one subjected to euthanasia and one having a third bursoscopic lavage prior to discharge. Overall, 18/24 (75%) followed up cases RTW, 10 (55.5%) from the needle group, eight (44.4%) the bursoscopy group. No statistically significant differences between first surgical technique used and success at first surgery (no subsequent lavage(s) required), survival to discharge or return to work were detected. The main limitations of this study are that it is a retrospective study, has a small population with limited statistical power and potential selection bias. No statistically significant differences existed between the outcomes of the two techniques, contrary to the belief that bursoscopic lavage is superior. Larger, multicentred studies, with greater statistical power are required to further assess this relationship.  相似文献   

耐热小麦生理特征分析     

吕丽华  李谦  雷明帅  姚艳荣  贾秀领 《华北农学报》2020,35(z1):138-144

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