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排序方式： 共有92条查询结果，搜索用时 15 毫秒
1.
Robert-Matthias Leiser 《Genetic Resources and Crop Evolution》1989,37(1):57-78
Die Entwicklung der Gentechnik führte in jüngster Zeit zu Möglichkeiten, ökonomisch interessante Merkmale von Kulturpflanzen zu manipulieren. Hierzu zählt die Krankheitsresistenz und speziell die Virusresistenz. Da das Virushüllprotein eine herausragende Rolle bei der in der Pflanzenvirologie seit langem bekannten Erscheinung der Prämunität zu spielen scheint, wurde durch mehrere Gruppen versucht, die Hüllproteingene verschiedener Pflanzenviren in deren Wirtspflanzen zu transferieren und zur Expression zu bringen. Die transgenen Hüllprotein-exprimierenden Pflanzen erwiesen sich in ähnlicher Weise als virusresistent wie prämunisierte Pflanzen.Eine zweite Strategie zur Beeinflussung der Virusresistenz betrifft die Nutzung des antisense-RNA-Prinzips. Allerdings waren die bisher beschriebenen Effekte immer weitaus geringer als jene im Falle der Hüllproteingen-Expression. Offensichtlich wurden noch nicht die sensitiven Targetsequenzen getroffen, zu denen in erster Linie Ribosomen- und Replikase-Bindungsstellen gehören dürften. Zwei weitere Strategien zur Manipulation der Virusresistenz wurden in letzter Zeit diskutiert und in ersten Modellversuchen geprüft: 相似文献
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R. Leiser 《Anatomia, histologia, embryologia》1975,4(1):63-86
Development of contact between trophoblast and uterine epithelium during the early stages of implantation in the cow Implantation in the cow may be divided into precontact, apposition, and adhesion phases. These can be recognized grossly at the site of nidation (18 or 19 days post inseminationem) by changes in the endometrium and in the embryonic chorion, which differs from the extraembryonic chorion. Also light and electronmicroscopical examinations of the trophoblast and uterine epithelium during the course of nidation show typical phase-correlated differences. The surfaces of trophoblastic and uterine epithelia increase and unite by interdigitiation of their microvilli. The functional relationship between blastocyst and uterus at the time of contact, as determined from their structure, is discussed. 相似文献
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Objective To measure the prevalence of megabacteria in budgerigar-breeding colonies and to evaluate possible methods to reduce the prevalence.
Design A monitoring study over several years.
Sample population Two budgerigar ( Melopsittacus undulatus ) colonies with over 300 birds each.
Procedure The prevalence of megabacteria in the faeces in two budgerigar breeding colonies, colony 1 and 2, was determined by faecal examination of each bird. Following an initial survey (1990), most of the birds that were scored 2+ or more were culled and a management practice was implemented to discriminate against positive birds. Consecutive yearly surveys (1991, 1992) were conducted on the young birds bred in these colonies. The prevalence of megabacteria in colony 2 was also evaluated in 1994 and 1996 after all the birds were treated with amphotericin B administered in drinking water.
Results The prevalence of megabacteria in the two colonies was significantly (P < 0.001) different. Overall the prevalence of megabacteria adjusted for colony differences was significantly higher (P < 0.025) in males compared to females. Age was not an influencing factor. After the initial survey, the prevalence in the offspring did not significantly (P > 0.05) decrease in the following two annual breeding seasons but by inference it did significantly decrease after amphotericin B treatment.
Conclusion The practice of culling most birds with more megabacteria in faeces and discriminating against positive birds when selecting birds for breeding or culling birds on show quality does not decrease megabacteria prevalence in the offspring. However, a reduction in prevalence does occur with administration of amphotericin B. Birds may have amphotericin B-resistant organisms and these birds need to be identified and culled. 相似文献
Design A monitoring study over several years.
Sample population Two budgerigar ( Melopsittacus undulatus ) colonies with over 300 birds each.
Procedure The prevalence of megabacteria in the faeces in two budgerigar breeding colonies, colony 1 and 2, was determined by faecal examination of each bird. Following an initial survey (1990), most of the birds that were scored 2+ or more were culled and a management practice was implemented to discriminate against positive birds. Consecutive yearly surveys (1991, 1992) were conducted on the young birds bred in these colonies. The prevalence of megabacteria in colony 2 was also evaluated in 1994 and 1996 after all the birds were treated with amphotericin B administered in drinking water.
Results The prevalence of megabacteria in the two colonies was significantly (P < 0.001) different. Overall the prevalence of megabacteria adjusted for colony differences was significantly higher (P < 0.025) in males compared to females. Age was not an influencing factor. After the initial survey, the prevalence in the offspring did not significantly (P > 0.05) decrease in the following two annual breeding seasons but by inference it did significantly decrease after amphotericin B treatment.
Conclusion The practice of culling most birds with more megabacteria in faeces and discriminating against positive birds when selecting birds for breeding or culling birds on show quality does not decrease megabacteria prevalence in the offspring. However, a reduction in prevalence does occur with administration of amphotericin B. Birds may have amphotericin B-resistant organisms and these birds need to be identified and culled. 相似文献
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Objective To estimate the changes in productivity and profitability in a group of wool-growing farms as they adopted major recommendations from agricultural and veterinary studies.
Farms Four wool-growing farms in south western Victoria were selected from the clients of the Mackinnon Project, a farm consultancy service run from the University of Melbourne. Each farm had closely followed recommended procedures, kept comprehensive financial and physical records and had been clients for at least 5 years. The comparison group was the South Western Victoria Monitor Farm Project (SWVMFP), about 45 farms in the same region as the study farms that were monitored annually by Agriculture Victoria.
Procedure For a 7-year period, the financial and physical performance of both groups of farms was estimated. Stocking rate, wool production, gross farm income, farm operating costs, net farm income and return on assets were compared.
Results Mean gross farm income of the four study farms steadily rose from 86% of the average SWVMFP farm before the adoption of recommendations to an average of 155%. During the same period, net farm income rose from 70% to 207% of the average of the SWVMFP. Return on asset of the four farms rose irregularly from 26% to 145% of the average of the SWVMFP. Farm operating costs on the four farms were higher than for the SWVMFP group, but the ratio of costs remained relatively constant.
Conclusion The adoption of proven research results was associated with large increases in net farm income. An increase in gross income, rather than a reduction in costs was the main reason for this. Research results offer a way to increase the financial viability of wool-growing farmers, many of whom are currently unable to maintain their lifestyle, resources and infrastructure. 相似文献
Farms Four wool-growing farms in south western Victoria were selected from the clients of the Mackinnon Project, a farm consultancy service run from the University of Melbourne. Each farm had closely followed recommended procedures, kept comprehensive financial and physical records and had been clients for at least 5 years. The comparison group was the South Western Victoria Monitor Farm Project (SWVMFP), about 45 farms in the same region as the study farms that were monitored annually by Agriculture Victoria.
Procedure For a 7-year period, the financial and physical performance of both groups of farms was estimated. Stocking rate, wool production, gross farm income, farm operating costs, net farm income and return on assets were compared.
Results Mean gross farm income of the four study farms steadily rose from 86% of the average SWVMFP farm before the adoption of recommendations to an average of 155%. During the same period, net farm income rose from 70% to 207% of the average of the SWVMFP. Return on asset of the four farms rose irregularly from 26% to 145% of the average of the SWVMFP. Farm operating costs on the four farms were higher than for the SWVMFP group, but the ratio of costs remained relatively constant.
Conclusion The adoption of proven research results was associated with large increases in net farm income. An increase in gross income, rather than a reduction in costs was the main reason for this. Research results offer a way to increase the financial viability of wool-growing farmers, many of whom are currently unable to maintain their lifestyle, resources and infrastructure. 相似文献