小黑麦不同品种劣变种子的胚培养效果研究     

李雪  田新会  杜文华 《草原与草坪》2018,(1):76-82

试验选用从澳大利亚引进的10个小黑麦品种的劣变种子的胚在1/2MS+2%蔗糖+0.1%麦芽糖+0.5%琼脂、pH 5.8的培养基中进行培养。结果表明:劣变小黑麦品种种子胚的发育率和成苗率由高到低分别为:DH265Cherokee2090WhiteAT528WhileM96-3182-1AT754AT315和CherokeeDH2652090WhiteAT528WhileM 96-3182-1AT754AT315,试验材料的胚发育率和成苗率均显著低于正常小黑麦材料(CK1,CK2);小黑麦品种33rd ITSN,32rd ITSN和Rhino的胚发育率和成苗率均为0;劣变小黑麦种子进行胚培养时,诱导培养的适宜时间为27d。  相似文献   

运用机载LiDAR数据对橡胶林地上生物量估测的采样尺度效应分析     

罗洪斌  舒清态  庞勇  王强  王冬玲 《林业资源管理》2020,(1):136-142

气候变化下,森林生物量遥感监测是当前研究的热点,机载LiDAR作为重要的遥感信息源,其采样大小对生物量估测精度有着一定的影响。以机载LiDAR数据为信息源,以44块30m×30m的方形橡胶林实测样地数据为基础,对机载激光雷达数据进行不同尺寸采样(共21个采样尺寸,边长从10m至30m,间隔为1m),提取不同采样尺寸下的激光雷达参数,并与橡胶林地上生物量建立PLSR模型,就机载激光雷达采样大小对橡胶林地上生物量估测精度的影响进行研究。研究表明:当采样尺寸小于18m时,估测精度随着采样尺寸的增大而增大;而当采样尺寸大于18m时,估测精度随着采样尺寸的增大而减小,进而趋于平缓。结果虽然呈现出一定的规律性,但是差异并不是很明显。当采样尺寸为18m时估测效果最佳,模型决定系数(R^2)为0.718,均方根误差(RMSE)为17.830 t/hm^2;交叉验证精度P和RMSEcv分别为82.741%和18.874t/hm^2。相较于实际样地(30m)尺寸下的估测结果,18m采样尺寸下的R^2提高了1.989%,RMSEcv降低了2.611%。因此,生物量的估测精度受机载激光雷达数据采样尺寸大小的影响,在生物量估测过程中需结合研究对象和研究区的实际情况对采样尺寸进行选择,从而提高生物量估测精度。  相似文献   

马传贫驴白细胞弱毒疫苗株跨膜蛋白主要免疫决定区基因的克隆与表达   总被引：3，自引：0，他引：3  

薛飞  贾斌  朱远茂  杨建德  王晓钧  相文华  赵立平  吕晓玲  沈荣显 《中国预防兽医学报》2003,25(5):321-325

从感染驴白细胞的马传贫驴白细胞弱毒疫苗株前病毒DNA中克隆了编码跨膜蛋白主要免疫决定区(TMIR)的基因，并在大肠杆菌中进行了表达。所表达的融合蛋白有一部分是可溶的，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。在间接酶联免疫吸附试验(ELISA)和免疫印迹试验中，重组的TMIR蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。这表明该重组蛋白具有良好的抗原性和特异性，可用于马传贫弱毒疫苗株在体内外复制、接种马体内免疫应答及马传贫诊断的研究。  相似文献   

黄鳝消化道的组织学与组织化学研究   总被引：14，自引：0，他引：14  

朱有法  舒妙安  沈元新 《中国兽医学报》2002,22(3):256-259

用肠卷石蜡法 ,对幼鳝和成鳝的消化道全长作纵切片 ,HE染色显示消化器官组织结构 ,嗜银法显示消化道内分泌细胞。结果显示 ,黄鳝消化道为一直形管道 ,主要由食管、胃、前肠和后肠组成。消化道的组织结构与哺乳类有许多相似之处 ,基本上由 4层组成。但食管上皮为含有大量杯状细胞的复层上皮 ,除胃体部外 ,其余器官内均缺腺体。本研究还显示了各器官连接部的结构变化。上述器官内均有散在分布的消化道内分泌细胞 ,表明黄鳝消化道还是一个主要的内分泌器官  相似文献   

用生态经济型植物控制豚草的"绿色污染"   总被引：5，自引：0，他引：5  

周早弘  舒洪岚 《江西林业科技》2004,(1):37-39

豚草是一种严重危害农业生产、人体健康和生物环境的恶性杂草，是一种典型的“绿色污染”。根据群落演替规律，采用具有生态和经济功能的树种创造不利豚草生长的环境，人为创建多物种和谐共存的植物群落，能降低豚草的种群优势，使单一种群优势转变为良性演替的生态系统。  相似文献   

毛花雀稗混播草地建植技术初探   总被引：2，自引：0，他引：2  

何静  尚以顺  舒建虹 《四川草原》2005,(4):17-19

试验探讨了建植毛花雀稗混播草地的最佳草种组合和播种量,结果表明,毛花雀稗(30cm×30cm≈7000株)+黑麦草(每667m2为0.5kg)+白三叶(每667m2为0.15kg)在独山气候条件下的生产性能显著高于其他组合。  相似文献   

Effect of Yigu capsule drug-containing serum on apoptosis and tartrate resistant acid phosphatase secretion in rat osteoclasts     

SHU Xiao-chun  ZHANG Rong-hua  ZHU Xiao-feng  PENG Ke-ping 《园艺学报》2003,19(9):1234-1237

AIM:The effects of Yigu capsule on tartrate resistant acid phosphatase (TRAP) secretion and apoptosis in rat osteoclasts were investigated in order to further explore its mechanism of preventing and treating osteoporosis.METHODS:(1) Twenty-month-old Sprague-daweley rats were randomly divided into two groups(Yigu capsule group and saline group), and the drug-containing serum and control serum were prepared. (2) The newborn Sprague-daweley rat osteoclasts were cultured with different concentrations of Yigu capsule drug-containing serum. At different time point, TRAP activity was measured and the survival osteoclast was counted under reverse microscope.The percentage of osteoclast apoptosis was observed under fluorescence microscope after acridine orange staining.RESULTS:TRAP activity was lower and the percentage of osteoclast apoptosis was higher in drug-containing serum group than in control group at 24, 48 and 72 h(P<0.01), respectively, and the survival osteoclasts were less in drug-containing serum group than in control groups at 24, 48 and 72 h(P<0.01).CONCLUSIONS:These data suggest that Yigu capsule drug-containing serum induces apoptosis and inhibits TRAP activity in osteoclasts, which may be one of the mechanisms of Yigu capsule preventing and treating osteoporosis.  相似文献   

Effects of oxidized low density lipoprotein and lysophosphatidylcholine on cholesterol efflux from mouse peritoneal macrophage foam cells     

FENG Xiang  LING Wen-hua 《园艺学报》2003,19(9):1246-1249

AIM:To explore the effects of oxidized low density lipoprotein (OxLDL) and one of its component— lysophosphatidylcholine (LPC) on cholesterol efflux from mouse macrophage foam cells.METHODS:(1) Cholesterol efflux induced by apoAI from mouse peritoneal macrophage foam cells loaded with OxLDL or acylated LDL(AcLDL) was measured. (2) Cholesterol efflux induced by LPC and apoAI from macrophage foam cells separated from normal or apoE gene deficient (E⁰) mouse loaded with AcLDL were measured.RESULTS:(1) When the macrophage foam cells were incubated with apoAI, cholesterol efflux from AcLDL-induced macrophage foam cells increased significantly compared to that of OxLDL-induced macrophage foam cells. (2) LPC promoted cholesterol efflux from macrophage foam cells in relation to both dosage and time. When LPC was incubated with E⁰ mouse macrophage foam cells, the released cholesterol mass was significantly lower than that of normal mouse macrophage foam cells. It was also found that cholesterol efflux induced by apoAI normally occurred in E⁰ mouse macrophage foam cells.CONCLUSIONS:(1) OxLDL accumulated cholesterol in macrophages and impair cholesterol efflux. (2) LPC induced cholesterol efflux from macrophage foam cells, which may occur via apoE pathway.  相似文献   

金针菇果冻的研制   总被引：5，自引：0，他引：5  

吕嘉枥  舒国伟  秦俊哲  吴国稳 《中国食用菌》2003,22(5):47-48

以金针菇子实体或深层培养所获得的菌丝体为原料，以普通果冻加工工艺为基础，将金针菇与果冻有机结合，所制得的产品不仅色、香、味、形俱佳。而且营养丰富，健康性强，成本低廉，方便食用，是一种新型营养性果冻，消费市场广阔。该生产工艺同样适用于其它食用菌的加工。  相似文献   

猪链球菌病二价灭活疫苗候选株培养条件的优化及其安全性试验     

王卓  舒秀伟  王文成 《中国兽药杂志》2004,38(7):34-36

筛选对猪致病力强、免疫原性良好的猪链球菌病兰氏C群LN01株和猪链球菌Ⅱ型LN05株,通过优化培养条件,LN01株和LN05株培养菌数最高分别为1.2×109和2.8×109CFU/mL;LN01株未见溶血素产生,LN05株溶血素效价为1∶64;脱毒剂A(甲醛)对其毒素的脱毒作用不明显,37℃脱毒30d,对仔猪的安全剂量为5mL;脱毒剂B的脱毒时间缩短为3d,安全剂量增加到10mL;试验还证实,37℃和60℃脱毒温度对本菌液脱毒效果无明显差异.  相似文献