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Mannheimia granulomatis PCR检测方法的建立 总被引：3，自引：0，他引：3
Mannheimia granulomatis是导致牛、羊呼吸道传染病的一种病原菌.为快速、准确诊断由该菌导致的疫病,从基因库中获得M.granulomdtis的基因序列,再从M.granulomatis的基因序列中获得与其他细菌包括溶血性曼氏杆菌和多杀性巴氏杆菌等不同的基因片段,设计引物,建立了特异性好、敏感性较高的M.granulomatis的PCR诊断方法.结果表明,其特异性为100%,最小检出量为5×104cfu/mL～5×103cfu/mL M.granulomatis或1/10个单个菌落. 相似文献
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Steffen S. Madsen Stephen D. McCormick Graham Young Jeanette S. Endersen Richard S. Nishioka Howard A. Bern 《Fish physiology and biochemistry》1994,13(1):1-11
Several experiments were performed to investigate the physiology of seawater acclimation in the striped bass, Morone saxatilis. Transfer of fish from fresh water (FW) to seawater (SW; 31–32 ppt) induced only a minimal disturbance of osmotic homeostasis.
Ambient salinity did not affect plasma thyroxine, but plasma cortisol remained elevated for 24h after SW transfer. Gill and
opercular membrane chloride cell density and Na+,K+-ATPase activity were relatively high and unaffected by salinity. Average chloride cell size, however, was slightly increased
(16%) in SW-acclimated fish. Gill succinate dehydrogenase activity was higher in SW-acclimated fish than in FW fish. Kidney
Na+, K+-ATPase activity was slightly lower (16%) in SW fish than in FW fish. Posterior intestinal Na+,K+-ATPase activity and water transport capacity (Jv) did not change upon SW transfer, whereas middle intestinal Na+,K+-ATPase activity increased 35% after transfer and was correlated with an increase in Jv (110%). As salinity induced only minor changes in the osmoregulatory organs examined, it is proposed that the intrinsic euryhalinity
of the striped bass may be related to a high degree of “preparedness” for hypoosmoregulation that is uncommon among teleosts
studied to data. 相似文献
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Stomata regulate gas exchange and are distributed across the leaf epidermis with characteristic spacing. Arabidopsis stomata are produced by asymmetric cell divisions. Mutations in the gene TOO MANY MOUTHS (TMM) disrupt patterning by randomizing the plane of formative asymmetric divisions and by permitting ectopic divisions. TMM encodes a leucine-rich repeat-containing receptor-like protein expressed in proliferative postprotodermal cells. TMM appears to function in a position-dependent signaling pathway that controls the plane of patterning divisions as well as the balance between stem cell renewal and differentiation in stomatal and epidermal development. 相似文献
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Elisabeth K. Olsen Espen Hansen Johan Isaksson Jeanette H. Andersen 《Marine drugs》2013,11(8):2769-2784
Three known bromophenols, 2,3-dibromo-4,5-dihydroxybenzylaldehyde (1), 2,2′,3-tribromo-3′,4,4′,5-tetrahydroxy-6′-hydroxymethyldiphenylmethane (2) and bis(2,3-dibromo-4,5-dihydroxylbenzyl) ether (3), and one new one, 5,5″-oxybis(methylene)bis(3-bromo-4-(2′,3′-dibromo-4′,5′-dihydroxylbenzyl)benzene-1,2-diol) (4), were isolated from an extract of the red alga, Vertebrata lanosa. The antioxidant activity of these four bromophenols was examined using one biochemical and two cellular assays: Oxygen Radical Absorbance Capacity (ORAC), Cellular Antioxidant Activity (CAA) and Cellular Lipid Peroxidation Antioxidant Activity (CLPAA) assays. Compound 2 distinguished itself by showing potent activity, having a better antioxidant effect than luteolin in both the CAA and CLPAA assays and of quercetin in the CLPAA assay. Although several bromophenols are known to be potent antioxidants in biochemical assays, this is the first time their cellular antioxidant activity has been demonstrated. 相似文献
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Extract Sir:- In June 1982, possums (Trichosurus vulpecula) from the Waitahuna forest area near Lawrence in Central Otago were examined for endoparasites (181 possums) and ectoparasites (125 possums). The alimentary tract was separated into stomach, small intestine and large intestine. These sections were washed thoroughly through a 60 mesh sieve and total parasite counts and identification were carried out. The fur from the base of the forelegs along the back and around the base of the tail was digested in 20% KOH and the ectoparasites were recovered. There was no evidence of pathogenicity ascribable to the parasites found. A summary of results is presented in Table I. 相似文献
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Janice K Gard William C Hutton Jeanette A Baker RK Singh Paul CC Feng 《Pest management science》1999,55(2):215-217
While the use of NMR and stable isotopes in metabolism studies is hardly new, it is only recently that isotope-edited NMR spectroscopy has been applied in kinetic studies of glyphosate metabolism of soil microbes. NMR can detect multiple species simultaneously and non-destructively, yielding valuable information on structural identification of metabolites. T riple R esonance I sotope ED ited spectroscopy (TRIED), [2H]NMR, and [2H–13C] INEPT (I nsensitive N ucleus E nhancement through P olarization T ransfer) are three isotope-edited techniques which have been used in combination to examine the microbial degradation of glyphosate (N-phosphonomethylglycine). Using 13C- and 15N-labeled glyphosate, TRIED can detect multiple metabolites in crude matrices at submicrogram levels, an improvement over earlier techniques where milligrams were needed. It can detect 500 nanograms of 13C–15N-labeled compound in a crude sample (1 : 1400 mass ratio), only a few hours work being required. [2H]NMR and [2H–13C]INEPT were also used as complementary techniques to further examine metabolites whose 13C–15N bond has been cleaved. The three-isotope-edited methods produced results consistent with both radioactivity and HPLC analyses. Accordingly, we are able to detect minute levels of metabolites in the presence of complex mixtures, minimizing the costs and time of sample purification. ©1999 Society of Chemical Industry 相似文献
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The properties of a white wheat bread could be changed by adding normal or heat‐treated barley flour in small amounts (2 and 4%) to a white wheat bread recipe. Differences regarding gelatinization as well as retrogradation properties were found when analyzing the two flours in model systems. The heat‐treated flour was fully gelatinized due to prior time, temperature, and pressure treatment and could therefore absorb larger amounts of water than the other flours. In gelatinized model systems with 40% flour (dwb), the heat‐treated barley flour contained less retrograded amylopectin as compared with normal barley flour after storage for up to 14 days, whereas no differences were found with 20% flour (dwb). However, stored breads showed an increased retrogradation of amylopectin (as measured by differential scanning calorimetry [DSC]) when 2% pretreated barley flour was added as compared with addition of 2% normal barley flour. On the other hand, there were no significant differences at the 4% level. Addition of either of the barley flours resulted in less firm breads during storage as compared with the control breads. Increased water absorption in barley flour and thus increased water content in the breads or different water‐binding capacities of the flour blends could explain these results. The present study indicated that water had a stronger influence on bread firmness than the retrogradation of amylopectin. This conclusion was based on breads with pretreated barley flour being less firm than breads with normal barley flour, although the retrogradation, as determined by DSC, was higher. 相似文献
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Inclusion of camelina meal as a protein source in diets for farmed Atlantic cod Gadus morhua 
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下载免费PDF全文 Stefanie M Hixson Christopher C Parrish Jeanette S Wells E Marie Winkowski Derek M Anderson 《Aquaculture Research》2016,47(8):2607-2622
Camelina meal Camelina sativa (CM) is a potential protein source in aquaculture feeds, because of its crude protein level (39%) and essential amino acids. Two feeding experiments were conducted with Atlantic cod Gadus morhua. Cod in Experiment I (19.4 g fish?1) were fed diets with 0%, 12% or 24% CM for 9.5 weeks at 10°C; and cod in Experiment II (14.4 g fish?1) were fed diets with 0%, 15%, 30% or 40% CM for 13 weeks at 10°C. Growth, lipid and amino acid tissue composition were compared amongst cod fed varying levels of CM. In Experiment I, cod could tolerate the highest level of CM inclusion (24%) without affecting growth compared to cod fed the control diet. In Experiment II, growth performance was significantly affected at 30% CM inclusion compared to the control treatment, and cod fed 15% CM displayed some signs of depressed growth (reduced feed intake and weight gain). Both treatment and duration were interacting factors (P = 0.015) that determined growth performance when comparing both experiments. Muscle tissue composition was relatively unaltered with less than 30% CM inclusion; however, multivariate statistics revealed significant differences in muscle tissue fatty acid composition between cod fed 40% CM and the control diet. The tissue amino acid profile was generally unaltered because the dietary amino acid profile was consistent after CM inclusion. A few antinutritive compounds in CM may have affected palatability in diets with greater than 30% CM inclusion, which may have resulted in reduced growth performance. 相似文献