全文获取类型
收费全文 | 6001篇 |
免费 | 224篇 |
国内免费 | 388篇 |
专业分类
林业 | 346篇 |
农学 | 479篇 |
基础科学 | 281篇 |
610篇 | |
综合类 | 2489篇 |
农作物 | 341篇 |
水产渔业 | 226篇 |
畜牧兽医 | 1148篇 |
园艺 | 512篇 |
植物保护 | 181篇 |
出版年
2024年 | 15篇 |
2023年 | 75篇 |
2022年 | 289篇 |
2021年 | 321篇 |
2020年 | 260篇 |
2019年 | 275篇 |
2018年 | 173篇 |
2017年 | 317篇 |
2016年 | 215篇 |
2015年 | 369篇 |
2014年 | 301篇 |
2013年 | 387篇 |
2012年 | 526篇 |
2011年 | 575篇 |
2010年 | 522篇 |
2009年 | 460篇 |
2008年 | 419篇 |
2007年 | 347篇 |
2006年 | 254篇 |
2005年 | 185篇 |
2004年 | 92篇 |
2003年 | 63篇 |
2002年 | 34篇 |
2001年 | 35篇 |
2000年 | 31篇 |
1999年 | 17篇 |
1998年 | 10篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 9篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 4篇 |
1981年 | 1篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1968年 | 2篇 |
1956年 | 2篇 |
排序方式: 共有6613条查询结果,搜索用时 14 毫秒
1.
通过对川西北地区的老井进行复查,发现多口井在上二叠统吴家坪组存在大套的凝灰岩,对上述井的地质、测井、地震资料综合分析发现,凝灰岩的岩性以凝灰质泥岩、凝灰质粉砂岩、凝灰质灰岩、沉凝灰岩、凝灰岩、凝灰质白云岩为主,次生溶蚀孔隙发育;凝灰岩在测井曲线和地震剖面上均有明显的响应特征:在测井曲线上表现为明显的低纵波速度、高自然伽马特征,凝灰岩底界在地震剖面上均表现为波峰反射特征.为此,采用模型正演分析技术及波阻抗反演技术,首次对四川盆地川西北地区晚二叠世吴家坪组凝灰岩的分布进行了地震预测:双鱼石构造-剑阁区块的北部地区凝灰岩分布最厚,南部减薄;双鱼石构造东部凝灰岩分布较厚,西部减薄,直至不发育.研究结果表明,晚二叠世吴家坪组在川西北地区沉积了一套凝灰岩,从西北至东南方向减薄;由于凝灰岩物性好,孔隙度高,可成为四川盆地火山岩气藏勘探全新的后备领域,为川西北地区下一步新的勘探领域开发提供了重要研究基础. 相似文献
2.
Shi-Zhi Wang Guang-Xin E Yan Zeng Yan-Guo Han Yong-Fu Huang Ri-Su Na 《Reproduction in domestic animals》2021,56(6):936-941
The aim of this study was to analyse the association between single-nucleotide polymorphisms within INHA and ACVR2B and litter size in Dazu black goats. In total, twenty-two SNPs were genotyped in 190 individuals by SNaPshot and resequencing. The results showed that three SNPs (SNP_1, SNP_12 and SNP_13 in this study) were detected to have significant additive genetic effect on the recorded goat litter size (p < .05). The SNP_1 (NC_030809.1), a non-synonymous substitution of G for T at chr2-g. 28314990 in the exon 2 of INHA gene (NM_001285606.1), resulted in homozygote 2 (HOM2) contributed 0.25 and heterozygote (HET) contributed 0.12 larger litter than homozygote 1 (HOM1). Meanwhile, SNP_12 (Chr22-g. 11721225 A > T) and SNP_13 (Chr22-g. 11721227 A > C) (NC_030829.1) simultaneously mutated at the first and third position of a triplet AAA (lysine, K) in the exon 4 of ACVR2B gene (XM_018066623.1) had estimated genetic effects of HOM1 (0.00) and HOM2 (0.03) larger than HET (−0.12). In conclusion, one SNPs (chr2-g. 28314990 T > G) within the exon 2 of INHA and two SNPs (Chr22-g. 11721225 A > T and Chr22-g. 11721227 A > C) i n the exon 4 of ACVR2B gene were highly recommended as candidate markers of litter size in Dazu black goats. A large-scale association study to assess the impact of these variants on litter size is still necessary. 相似文献
3.
前期研究中通过对秀珍菇经低温诱导后不同发育阶段样本进行转录组测序及差异表达基因分析发现,ID为Cluster-6377.64510的基因(命名为PpFBD1)在原基形成前期的样本中高表达。为进一步了解分析其表达特性及功能,根据转录组测序结果设计特异性引物,利用RT-PCR技术从秀珍菇中克隆获得了该基因的cDNA全长。该基因由342个核苷酸组成,编码一个由113个氨基酸组成的蛋白,蛋白相对分子质量约11 ku。进化树分析显示,PpFBD1编码蛋白与糙皮侧耳hydrophobin1编码的疏水蛋白亲缘关系最近。Gene Ontology功能分析表明,PpFBD1主要参与真菌类细胞壁的合成。荧光定量RT-PCR检测显示,该基因在菌丝体经低温诱导后恢复至室温阶段(原基形成前期)表达量最高,这表明其可能参与调控秀珍菇原基形成过程。本研究结果为阐明秀珍菇原基形成机制提供参考。 相似文献
4.
Fang Chen Hao Zhang Na Zhao Xuehai Yang Encun Du Shaowen Huang Wanzheng Guo Wei Zhang Jintao Wei 《Animal Science Journal》2021,92(1):e13619
Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA300 group and HS + CGA600 group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA300, and HS + CGA600 group were exposed to 38°C for 4 h while the control group was maintained at 25°C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1β and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition. 相似文献
5.
6.
随着种子市场的规范化、商品化程度的提高,种子质量意识的大大增强,建立健全种子质量保障制度,全面保证种子质量,确保农民增产,农民增收势在必行。1质量就是生命对于种子这一特殊商品来说更是至关重要的,只有保障种子质量,才能使种子行业长久不衰。企业不能为了片面追求经济效益,生产经营伪劣种子,这样不仅降低了种子公司的信誉,而且给农民造成了经济损失。2要严格按照规程操作,严把质量关在种子生产过程中,确保亲本种子质量,尤其是纯度。应及时解决制种过程中花期不遇,制种产量低的技术难题,并认真开展种子田间检验及室内检验,确保种子的纯… 相似文献
7.
稀土对阿尔巴斯白绒山羊瘤胃发酵,消化代谢及其生产性能的影响 总被引:5,自引:0,他引:5
实验1用4只安装永久性瘤胃漏管的阿尔巴斯白绒山羊(母),进行瘤胃消化代谢试验;实验2用6只成年母绒山羊进行消化代谢试验;试验3选用同品种成年妊娠母羊进行放牧补饲实验。实验1得出,稀土的使用可促进瘤胃内NH3-N的利用;细菌数比对照组提高35.67%;丙酸产量提高28.63%。实验2得出,稀土可提高绒山羊日粮DM、OM、GE及ADF消化率,分别为11.58%、12.27%、16.69%和26.29%;每日氮存留率比对照组提高180.1%。实验3得出,稀土组产绒量比对照组提高8.90%。 相似文献
8.
9.
采用迅速烘干、太阳晒干和缓慢烘干3种处理对云南温带21个牧草品种的鲜草进行干燥处理,分别测定干草的营养成分。结果表明:以干草的粗蛋白含量为指标,干燥方法以迅速烘干为佳,其次是太阳晒干,最差是缓慢烘干;从干草的粗脂肪考虑,其变化无规律,因牧草品种的不同而不同;其余指标变化不大。 相似文献
10.
AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer. 相似文献