Virus seeks its disease: a seroepizootiological study of the presence of bovine herpes mammillitis in Switzerland]     

M Engels  A Metzler  R Wyler 《Schweizer Archiv für Tierheilkunde》1979,121(11):565-576

  相似文献   

Transduction of Vero cells and bovine monocytes with a herpes simplex virus-1 based amplicon carrying the gene for the bovine herpesvirus-1 Circ protein     

Schwyzer M  Fischer-Bracher C  Fraefel C  Bächi T  Nuñez R  Engels M  Ackermann M 《Veterinary microbiology》2002,86(1-2):165-174

Herpes simplex virus-1 (HSV-1) based amplicon vectors are promising gene delivery vehicles because they have a large transgene capacity and can efficiently transduce many different cell types, including non-dividing cells, of various animal species. The Circ protein of bovine herpesvirus-1 (BHV-1) is a myristylated virion component of unknown function. Preliminary experiments with a circ gene deletion mutant indicated that Circ may influence the host's immune response by downregulating MHC-II expression in bovine monocytes. To get more insight into the function of Circ, amplicon vectors were constructed with various open reading frames (ORFs) under the control of the HSV-1 IE4/5 promoter: (i) the Circ ORF alone, (ii) a fusion ORF encoding an N-terminal Circ fused to the enhanced green fluorescent protein (eGFP), (iii) the eGFP ORF alone, and (iv) the Circ ORF in the inverted orientation. Upon helpervirus-free packaging into HSV-1 amplicon particles and transduction of Vero cells, both Circ alone and the Circ-eGFP fusion protein produced a punctate pattern within the cytoplasm, suggesting membrane association of the myristylated protein. In contrast, eGFP alone was evenly distributed over the cytoplasm of transduced cells. Upon infection of bovine buffy-coat cells, it was observed that cells of the monocyte lineage but not lymphocytes were transduced. Transgene expression reached a peak around 20h after transduction and lasted for at least 90h. Transduced monocytes underwent specific morphological changes, which may be attributed to Circ synthesis.  相似文献   

一例猪附红细胞体与弓形体混合感染的诊治     

崔双保  和彩红 《中国兽药杂志》2006,40(3):58-59

附红细胞体病是由立克次氏体引起的一种散在的热性、溶血性入畜共患传染病，病猪以急性黄疸性贫血和发热为特征。弓形体病是由刚地弓形虫在多种动物和入的有核细胞内寄生引起的一种入畜共患的原虫病。本文通过对某猪场发生猪附红细胞体与弓形体混合感染的诊治，从发病情况、临床症状、剖检变化、实验室诊断、治疗等方面对该病进行了阐述。  相似文献   

A model ensemble approach to determine the humus building efficiency of organic amendments in incubation experiments     

Anton A. Gasser  Julius Diel  Kerstin Nielsen  Paul Mewes  Christof Engels  Uwe Franko 《Soil Use and Management》2022,38(1):179-190

Organic amendments are important to sustain soil organic matter (SOM) and soil functions in agricultural soils. Information about the contribution of organic amendments to SOM can be derived from incubation experiments. In this study, data from 72 incubated organic amendments including plant residues, digestates and manure were analysed. The incubation data was compiled from three experimental setups with varying incubation times, soils and incubation temperatures, in which CO₂ release was measured continuously. The analysis of the incubation data was performed with an approach relying on conceptual parts of C-TOOL, CCB, Century, ICBM, RothC and Yasso which are all well-approved first-order carbon models that differ in structure and abstraction level. All models are an approximation of reality, whereby each model differs in understanding of the processes involved in soil carbon dynamics. To accumulate the advantages from each model a model ensemble was performed for each substrate. With the ability of each carbon model to compute the distribution of carbon into specific SOM pools a new approach for evaluating organic amendments in terms of humus building efficiency is presented that, depends on the weighted model fit of each ensemble member. Depending on the organic substrate added to the soil, the time course of CO₂ release in the incubation studies was predicted with different accuracy by the individual model concepts. Averaging the output of the individual models leads to more robust prediction of SOM dynamics. The E_HUM value is easy to interpret and the results are in accordance with the literature.  相似文献   

Prevalence of Campylobacter jejuni and Salmonella during pig slaughtering     

J Oosterom  R Dekker  G J de Wilde  F van Kempen-de Troye  G B Engels 《The Veterinary quarterly》1985,7(1):31-34

It was found that 79% of healthy pigs, slaughtered in three different slaughterhouses in the Netherlands, were intestinal carriers of Campylobacter jejuni (mean number 4000 cfu per g), and 21% of the same pigs had Salmonella in the intestinal tract (mean number 10 cfu per g). Immediately after slaughter, Campylobacter was swabbed from 9% of the carcasses and Salmonella from 13%. It is concluded from these data that most of the contamination on carcasses does not originate directly from the intestinal tracts of the animals but rather from surfaces, equipment, and utensils in the slaughter hall. It was demonstrated that Salmonella could survive in the slaughter hall, whereas Campylobacter died off, probably due to its vulnerability to drying conditions and its inability to grow at temperatures below 30 degrees C. Campylobacter was not isolated from the carcasses after cooling. It had been shown earlier that this again was caused by dry conditions, brought about by the use of forced ventilation in the cooling rooms. In an additional investigation, Campylobacter was not isolated from 248 samples of minced pork (10 g each), whereas Salmonella was found in 13% of these samples.  相似文献   

Sources and inheritance of resistance to stemphylium leaf spot of lettuce     

D. Netzer  D. Globerson  CH. Weintal  R. Elyassi 《Euphytica》1985,34(2):393-396

Summary None of the tested cultivars of lettuce was found resistant to Stemphylium leaf spot, a common disease in Israel. Within a Lactuca saligna population collected in wild lettuce in Israel, resistance was traced. Interspecific crosses of L. saligna x L. sativa were made and the mode of inheritance of resistance to this disease was studied. Resistance is apparently controlled by two genes: one dominant (Sm₁) and one recessive (sm₂).Contribution No. 1176-E 1984 series, from the ARO.  相似文献   

A systematic description of cacao clones. II. The discriminative value of qualitative characteristics and the practical compatability of the discriminative value of quantitative and qualitative descriptors     

J. M. M. Engels 《Euphytica》1983,32(2):387-396

  相似文献   

A manual system for germplasm data management. I. Description of the edge-punched card system     

J. M. M. Engels 《Euphytica》1981,30(3):629-634

  相似文献   

Chemical conversion of alpha-keto acids in relation to flavor formation in fermented foods     

Smit BA  Engels WJ  Alewijn M  Lommerse GT  Kippersluijs EA  Wouters JT  Smit G 《Journal of agricultural and food chemistry》2004,52(5):1263-1268

Formation of flavor compounds from branched-chain alpha-keto acids in fermented foods such as cheese is believed to be mainly an enzymatic process, while the conversion of phenyl pyruvic acid, which is derived from phenylalanine, also proceeds chemically. In this research, the chemical conversion of alpha-keto acids to aldehydes with strong flavor characteristics was studied, with the main focus on the conversion of alpha-ketoisocaproic acid to the aldehyde 2-methylpropanal, and a manganese-catalyzed reaction mechanism is proposed for this conversion. The mechanism involves keto-enol tautomerism, enabling molecular oxygen to react with the beta-carbon atom of the alpha-keto acid, resulting in a peroxide. This peroxide can react in several ways, leading to unstable dioxylactone or noncyclic intermediates. These intermediates will break down into an aldehyde and oxalate or carbon oxides (CO and CO(2)). All the alpha-keto acids tested were converted at pH 5.5 and in the presence of manganese, although their conversion rates were rather diverse. This chemical reaction might provide new ways for controlling cheese flavor formation with the aim of acceleration of the ripening process or diversification of the flavor characteristics.  相似文献   

小麦D~2型细胞质光敏雄性不育性的研究     

于天峰  刁艳玲  车京玉  迟永琴  李长辉  王岩  邵立刚  张爱民  鄂立柱 《东北农业大学学报》2002,33(4):324-330

D2型细胞质与有些普通小麦材料的细胞核互作,可产生特异核质互作不育现象,其中有的表现为长光照敏感型雄性不育。文章研究了具有D2型细胞质的光敏不育系Ae.crassa—Norin26、核质杂种NC2134及克引11、克引12在黑龙江省克山地区自然条件下的育性表现,并以NC2134为细胞质供体,与20个小麦品种(系)杂交,获得一些核质代换系材料,对其育性及主要性状进行观察研究。结果表明:(1)在自然条件下,播种期对育性有影响,随着播种期推迟,Ae.crassa—Norin26不育度增加,1 999年4月22日播种达到全不育,Norin26则在所设播种条件下表现正常可育;(2)NC2134表现可育,这与其可能携带雄性不育的恢复基因有关,克引11、克引12在正常播种情况下表现高度不育甚至全不育;(3)不育系的不育性表现为不同程度的雄蕊心皮化;(4)不同普通小麦材料其核质杂种育性表现不同,有些材料其核质代换低世代分离出一定比例的高不育、半不育及可育株,通过进一步的核置换,可转育成相应的D2型细胞质不育系。  相似文献