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Md. Shaheed Reza Nanami Mizusawa Ayaka Kumano Chiharu Oikawa Daisuke Ouchi Atsushi Kobiyama Yuichiro Yamada Yuri Ikeda Daisuke Ikeda Kazuho Ikeo Shigeru Sato Takehiko Ogata Toshiaki Kudo Mitsuru Jimbo Ko Yasumoto Kazutoshi Yoshitake Shugo Watabe 《Fisheries Science》2018,84(3):563-577
In an effort to determine genus- or species-level taxonomic profiles and diversity of bacterial consortia in the Tama River around urban Tokyo, next-generation sequencing technology targeting a 16S ribosomal RNA (rRNA) gene amplicon was employed. Metagenomic analysis performed by an Ion Personal Genome Machine after sequentially filtering samples through 5-, 0.8- and 0.2-μm filters yielded 1.48 Gb of 16S sequences (average 2.38 M reads/sample). The results indicated that half of the bacterial sequences belonged to Proteobacteria, followed by Bacteroidetes, Actinobacteria and Cyanobacteria. Flavobacterium (Bacteroidetes), possibly including a potential fish pathogen, was the most numerous genera in the Tama River metagenome, and accounted for?~?16% of assigned 16S reads, followed by Mycobacterium. Other dominant bacterial genera including Zoogloea, Sediminibacterium, Hyphomicrobium, Sphingopyxis, Thiothrix and Lysobacter, were thought to be associated with waste water and sludge. MiSeq metagenomic analysis revealed that environmental factors, particularly water temperature, influenced the bacterial composition throughout the year, with a strong negative correlation observed for Proteobacteria and a positive correlation for Bacteroidetes. In terms of bacterial genera, Flavobacterium was positively correlated with temperature, while Polaromonas, Pseudomonas and Bradyrhizobium were negatively correlated with this, suggesting dynamic change in the free-living bacterial population throughout the year and versatile adaptation strategies in relation to environmental factors. 相似文献
2.
Ikari Takahiro Kobayashi Yuki Kitani Yoichiro Sekiguchi Toshio Endo Masato Kambegawa Akira Asahina Kiyoshi Hattori Atsuhiko Tabuchi Yoshiaki Amornsakun Thumronk Mizusawa Kanta Takahashi Akiyoshi Suzuki Nobuo 《International Aquatic Research》2018,10(3):283-292
International Aquatic Research - The effects of α-melanocyte-stimulating hormone (α-MSH) on calcium metabolism were examined with goldfish. The scales on the left side of goldfish bodies... 相似文献
3.
Ken Touhata Atsushi Namikoshi Tamami Suzuki Jun Iguchi Nanami Mizusawa Tatsuro Hara Shintaro Imamura Takeshi Yabu Yumiko Yamashita Michiaki Yamashita 《Fisheries Science》2013,79(5):865-875
Nucleotide sequences in internal transcribed spacer (ITS)-1 region derived from dried nori products produced in Japan, China, and the Republic of Korea were compared. Thalli contained in the Japanese products were genetically homogenous, and their nucleotide sequences in ITS-1 were identical to those of the reference strains of Pyropia yezoensis f. narawaensis. In Chinese products, the thalli were related to P. yezoensis strain Minomiasakusa. In contrast, the thalli in the Korean products were genetically heterogeneous, and several different P. yezoensis strains and other Pyropia spp. were used for dried nori products. In some thalli produced in both China and Korea, the DNA sequences of the ITS-1 region were identical with that of Japan, suggesting that the cultivar strains might have been transplanted from Japan to China in recent years. The 432-bp-long nucleotide sequences in the ITS-1 region of thalli derived from Japanese origin were cleaved to two restriction fragments at 154 and 278 bp by cleavage of PCR-amplified products using MspI. Conversely, almost all of the corresponding sequences derived from China and Korea were lacking MspI or other restriction patterns, except for nori products from some areas that cultivate a closely related strain to the Japanese cultivar. 相似文献
4.
Yoshikawa Y Horiuchi M Ishiguro N Kadohira M Kai S Mizusawa H Nagata C Onodera T Sata T Tsutsui T Yamada M Yamamoto S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):959-968
The Food Safety Commission (FSC) of Japan, established in July 2003, has its own initiative to conduct risk assessments on food stuffs known as "self-tasking assessment". Within this framework, the FSC decided to conduct a risk assessment of beef and beef offal imported into Japan from countries with no previous BSE reports; thus, a methodology was formed to suit to this purpose. This methodology was partly based on the previous assessments of Japanese domestic beef and beef imported from U.S.A./Canada, but some modifications were made. Other organizations' assessment methods, such as those used for BSE status assessment in live cattle by the OIE and EFSA's GBR, were also consulted. In this review, the authors introduce this alternative methodology, which reflects (1) the risk of live cattle in the assessed country including temporal risks of BSE invasion and domestic propagation, with the assessment results verified by surveillance data, and (2) the risk of beef and beef offal consisting of cumulative BSE risk by types of slaughtering and meat production processes implemented and the status of mechanically recovered meat production. Other possible influencing factors such as atypical BSE cases were also reviewed. The key characteristic of the current assessment is a combination of the time-sequential risk level of live cattle and qualitative risk level of meat production at present in an assessed country. 相似文献
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The self-feeding abilities of individually reared rainbow trout Oncorhynchus mykiss were investigated under four different light intensities (7×10−2, 1×10°, 5×101, and 7×102 lx). There was no significant effect of light intensity on self-feeder learning ability and food wastage (which did not exceed
0.5%). However, there was high individual variability in the ability irrespective of light intensity. The total number of
trigger actuations was significantly higher at 1×10° lx than at 7×10−2 and 5×101 lx. Feeding patterns during the light phase of a day were classified as early or late in relation to the timing of significant
peaks in fish feeding, or non-steady. The last pattern was defined when significant peaks were not observed. Under 7×10−2, 1×10°, or 7×102 lx, a non-steady feeding pattern was the most common. Early feeding appeared only under the 1×10° lx regime with the random
pattern, while the late pattern was observed under 7×10−2, 5×101, and 7×102 lx. Under 5×101 lx regime half of the fish fed in the afternoon. These results suggest that light intensity can affect the timing of feeding
judged from the frequency of pulling bite tags, but does not affect waste or learning period. 相似文献
7.
Nomura R Shirai M Kato Y Murakami M Nakano K Hirai N Mizusawa T Naka S Yamasaki Y Matsumoto-Nakano M Ooshima T Asai F 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(7):885-891
Porphyromonas gulae, a gram-negative black-pigmented anaerobe, is a pathogen for periodontitis in dogs. An approximately 41-kDa fimbrial subunit protein (FimA) encoded by fimA is regarded as associated with periodontitis. In the present study, the fimA genes of 17 P. gulae strains were sequenced, and classified into two major types. The generation of phylogenetic trees based on the deduced amino acid sequence of FimA of P. gulae strains along with sequences from several strains of Porphyromonas gingivalis, a major cause of human periodontitis, revealed that the two types of FimA (types A and B) of P. gulae were similar to type I FimA and types II and III FimA of P. gingivalis, respectively. A PCR system for classification was established based on differences in the nucleotide sequences of the fimA genes. Analysis of 115 P. gulae-positive oral swab specimens from dogs revealed that 42.6%, 22.6%, and 26.1% of them contained type A, type B, and both type A and B fimA genes, respectively. Experiments with a mouse abscess model demonstrated that the strains with type B fimA caused significantly greater systemic inflammation than those with type A. These results suggest that the FimA proteins of P. gulae are diverse with two major types and that strains with type B fimA could be more virulent. 相似文献
8.
Watabe Shugo Ikeda Daisuke Mashiro Takaki Kagetakubo Yuko Takahashi Yoshihiro Uemura Misaki Mizusawa Nanami Koyama Hiroki Yasumoto Ko Jimbo Mitsuru Kan-no Nobuhiro Ueda Tomohiro Matsuoka Yoko Ueki Nobuhiko Wan Jianrong 《Fisheries Science》2020,86(4):711-719
Fisheries Science - Complementary DNA encoding a part of myosin heavy chain was cloned from fast skeletal muscle of Japanese codling Physiculus japonicus. Japanese codling, a frequent catch off the... 相似文献
9.
Kato Y Shirai M Murakami M Mizusawa T Hagimoto A Wada K Nomura R Nakano K Ooshima T Asai F 《Journal of veterinary dentistry》2011,28(2):84-89
Periodontal diseases are known to be major diseases in humans, and are also common in dogs. The purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. The distribution of an animal gingival organism Porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans, Campylobacter rectus, and Eikenella corrodens, were evaluated by polymerase chain reaction with species-specific sets of primers. Porphyromonas gulae, Tannerella forsythia and Campylobacter rectus were detected in almost all dogs analyzed, all of which should be regarded as common members of oral flora in dogs. Then, isolation and identification of the Porphyromonas species in swab specimens were performed. There were 35 strains isolated from 22 dogs, and broad-range polymerase chain reaction and sequencing methods revealed that approximately 70% of them were Porphyromonas gulae. In contrast, the frequency of Porphyromonas gingivalis was extremely low. These findings indicate the presence of specific periodontitis-related pathogens in pet dogs, especially Porphyromonas gulae. 相似文献
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