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1.
Aline F. S. Carvalho Monica R. Ferreira Machado Estefnia S. Andrade Luis D. S. Murgas Mrcio G. Zangeronimo Thiago C. Barros Fernanda G. Paula 《Journal of the World Aquaculture Society》2014,45(1):75-81
The aim was to evaluate the effect of different concentrations of caffeine added in activating solution over sperm motility in fresh and thawed semen of pacu, Piaractus mesopotamicus, and curimba, Prochilodus lineatus. The activating solutions were prepared with sodium bicarbonate solution of 0.76% (NaHCO3) and caffeine was added at concentrations of 2.5, 5.0, 10.0, and 20.0 mM. As control, a solution of NaHCO3 0.76 without caffeine was used. Eight males of pacu and 20 males of curimba were used. Aliquots of 200 μL of semen were diluted in 800 μL extender solution (DMSO 10% and BTS 5%), placed in 0.5 mL straws and cryopreserved for 7 d in a liquid nitrogen tank. There was a linear increase in sperm motility for fresh semen of pacu, and for curimba fresh and thawed semen (P < 0.05), due to the increase in the concentration of caffeine. There was a quadratic response for duration of motility for thawed semen of pacu and for fresh semen of curimba (P < 0.05), respectively. These results indicate that addition of caffeine in the activator solution can improve sperm motility parameters, however, is dependent on the species and concentration used. 相似文献
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Jeann Leal de Araújo Alice C. A. M. Arruda Nayadjala T. A. Santos Glenison F. Dias Thiago F. L. Nery Fabio Del Piero Richard Ploeg Brian F. Porter Ingeborg M. Langohr 《Journal of veterinary diagnostic investigation》2021,33(3):600
A 4-mo-old northern red-shouldered macaw (Diopsittaca nobilis) was admitted to the veterinary hospital of the Arruda Câmara Zoo, in the State of Paraiba, Brazil, for investigation of an orbital mass. Given rapid progression and lack of response to treatment, the bird was euthanized, and an autopsy was performed. Histologically, the mass consisted of a retrobulbar invasive tumor characterized by tubular and rosette-like structures, with interspersed heteroplastic tissues, such as aggregates of neuroglial cells and islands of hyaline cartilage. The tumor was immunopositive for pancytokeratin, GFAP, NSE, and S100. These findings were compatible with an ocular teratoid medulloepithelioma, a neoplasm best described in humans but also reported rarely in young cockatiels and African Grey parrots. 相似文献
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Thiago Luiz Da Mata Monique Inês SegerenAline Segeren Fonseca Carlos Augusto Colombo 《Scientia Horticulturae》2009
Genetic diversity was evaluated by RAPD markers and morpho-agronomic characters for a total of 42 accessions of Barberton daisy (Gerbera jamesonii) consisting of 29 commercial and 13 wild accessions. A total of 74 polymorphic bands were obtained employing a set of 12 primer pairs. The average genetic similarity coefficient for the 42 accessions, evaluated by Jaccard index was 0.55 ranging from 0.28 to 1.00. The genetic structure found among Barberton daisy accessions was evaluated by hierarchic classification analyses and UPGMA modeling, revealing six clusters of genotypes where two of them include the wild accessions and the remaining four including commercial material, except for wild genotype number 9. Shannon (H′) index was calculated using the molecular markers to investigate the genetic variation among the Gerbera accessions and showed higher values for the commercial cluster in comparison to the values obtained for the individuals from the non-commercial cluster, namely 0.34 versus 0.27, respectively. Therefore, both calculated indices (Jaccard and Shannon) indicated the presence of higher genetic variation among commercial accessions in comparison to the cluster representing non-commercial accessions, suggesting that genetic breeding programs may focus on commercial accessions to recombine interesting genotypes with commercially important and marketing-desired characteristics. 相似文献
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Robson RV Alves Tatiana Soares Elinaldo FL Bento Ricardo S Roldan‐Filho Brbara SS Souza Marcele KN Lima Jssica S Nascimento Luana CBB Coelho Roberto A S Thmarah A Lima Gabriel GA Gonalves Fbio A Brayner Luiz C Alves Daniela MAF Navarro Thiago H Napoleo Patrícia MG Paiva 《Pest management science》2020,76(2):730-736
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Lidiane Cristina Gonçalves de Sandre Hellen Buzollo Thiago Matias Torres do Nascimento Lígia Maria Neira Eduardo Gianini Abimorad Rosangela Kiyoko Jomori Carlos Ducatti Maria Célia Portella Dalton José Carneiro 《Journal of the World Aquaculture Society》2016,47(1):113-122
This study evaluated the application of stable isotopes of carbon as an alternative and more accurate method to determine gastrointestinal transit time (GTT) in fish by comparing it to the inert marker method. The stable isotope method detects alterations of the normal carbon flow in a biological system by analyzing naturally occurring isotopes of carbon, contrary to studies based on conventional techniques that apply external markers to the diet to determine GTT through visual observation of the color change in feces. Therefore, 320 pacu, Piaractus mesopotamicus juveniles were reared in 32 tanks under two different temperatures (25 and 29 C). The pacu juveniles received two different diets, one based on ingredients derived from C3 photosynthetic cycle plants and the other based on C4 plant ingredients, both containing titanium oxide (TiO2) as a marker. After 40 d, the isotopic signature of the diets was changed, and the marker was replaced by chromic oxide (Cr2O3). In the isotopic technique, the feces were analyzed to determine the exchange in the isotopic ratio of carbon δ13C. Both methods found that GTT was faster (nearly 6 h) in fish at 29 C when using the C4/C3 feeding strategy and slower in fish at 25 C using the C3/C4 strategy (15 h by inert marker and 18 h by the isotopic method). In conclusion, GTT determination in pacu juveniles using the stable isotope technique exhibits the same accuracy obtained with the inert marker method at temperatures suitable (nearly 29 C) for the metabolism of these animals. 相似文献
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de Andrade Thiago Cavalcante Gomes Ribeiro Bacha Allan Lopes de Camargo Mariane Bueno de Carvalho Leonardo Bianco 《New Forests》2022,53(1):143-160
New Forests - Previous studies have reported that phosphorus transporters are involved in glyphosate uptake by plants, so phosphorus fertilization might act as an attenuator of glyphosate effects... 相似文献
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Marcos Gallo Alfredo Trento Ana Alvarez Horacio Beldoménico Darío Campagnoli 《Water, air, and soil pollution》2006,174(1-4):367-384
The objective of this work is to analyze Fe, Mn, Cr, Cd, Cu, Pb, and Zn distributions in the water column in terms of total, dissolved, and particulate concentrations in the Salado River (Santa Fe – Argentina); their relationship with total, dissolved and suspended solids; and the impact of two pollution sources on the watercourse: a stream that collects domestic and industrial wastewater discharges of the region and a direct discharge that releases urban and industrial effluents to the river. Water samples obtained from 5 surveys were analyzed. The Salado River transported a load of heavy metals that ranged from 700 g/s to 2200 g/s of Fe, 20 g/s to 60 g/s of Mn and 1 g/s to 3 g/s of Cr. Results show that most metals were found in particulate form, that the metal input from the direct discharge may be significant at low water levels, whereas a lesser impact was produced by the stream, and that both pollution sources were negligible at high water levels. 相似文献
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Robson Thiago Xavier de Sousa Gaspar Henrique Korndörfer Rogério Augusto Brem Soares Patrícia Rezende Fontoura 《Journal of plant nutrition》2015,38(9):1444-1455
Phosphate applications are still rare in sugarcane cultivation and can be done with phosphorus fertilizers of residual over. This study aims to evaluate the agronomic efficiency of phosphate fertilization before sugarcane planting and its effect on sugarcane yield. The study was carried out over three crop cycles at the Jalles Machado sugar-mill in Goianésia, GO, Brazil. The treatments consisted of five different phosphorus sources (soluble and insoluble) applied at a rate of 300 kg ha?1 phosphorus pentoxide (P2O5). The phosphorus fertilizers used were: triple superphosphate, mono-ammonium phosphate (MAP), Arad rock phosphate, Itafós rock phosphate, and magnesium term phosphate. The effects of these fertilizers were evaluated by plant height (ground to Top Visible Dewlap; TVD), leaf phosphorus content, agronomic efficiency, and sugarcane yield from cane-plants, first ratoon, and second ratoon. Magnesium term phosphate and triple superphosphate resulted in greater plant-cane height. Leaf phosphorus concentrations were highest with applications of MAP, magnesium term phosphate, and triple superphosphate. Phosphate applications did not affect sugarcane plant yield, but did increase ratoon yield. In general, the residual effect of insoluble phosphate increased over the years. The agronomic efficiency (AE) of phosphate was (in descending order): triple superphosphate (100%), term phosphate (89%), MAP (80%), Itafós rock phosphate (67%), and Arad rock phosphate (60%). 相似文献