马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析     

唐锐敏  贾小云  朱文娇  印敬明  杨清 《作物学报》2021,(4):672-683

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Construction of Brucella abortus A19 VirB Promoter Deletion Strain and Study on Its Biological Characteristics     

DENG Xiaoyu  HE Jinke  YANG Qin  YI Jihai  WANG Yueli  XI Jing  WANG Zhen  CHEN Chuangfu 《中国畜牧兽医》2020,47(3):864-872

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拖拉机动量飞轮主动防侧翻控制与模型试验研究   总被引：1，自引：0，他引：1  

秦嘉浩  刘海帆  SUH C S  李臻  朱忠祥  王国业 《农业机械学报》2020,51(S2):515-520

针对拖拉机侧翻致死致伤事故仍时有发生的问题，基于旋转刚体加速时的反向施矩原理，以反作用动量飞轮为执行元件，提出了从主动安全角度解决拖拉机侧翻问题的研究方法。通过构建拖拉机动力学系统数学模型，解析了整机侧翻行为的动态演变机理。为保证拖拉机主体结构的完整性，将动量飞轮置于拖拉机前部，取代传统静态配重的同时可主动提供防侧翻力矩。应用Matlab/Simulink软件，对反作用飞轮的回稳过程进行了基于PID控制的有效性仿真分析，同时设计并搭建了比例模型试验平台，对主动施矩飞轮的回稳控制效果进行了试验验证。结果表明，装备飞轮的拖拉机与无控制组对比，在一次试验中可多次实现整机的防侧翻控制，使整机防侧翻性能得到明显改善，且不同行驶工况下的试验数据与仿真结果的相关性较强，充分验证了本文拖拉机侧翻动力学模型的有效性。  相似文献   

采前喷施氯化钙与醋酸钙对冬枣品质和贮藏性的影响     

王清华  杜振宇  杨守军  马海林  马丙尧  刘方春  王静 《中国土壤与肥料》2020,(1):141-146

钙对果树果实品质有重要改善作用,而品质又决定了其商品价值和种植效益。研究钙对冬枣品质和果实贮藏的影响作用,对于改善冬枣商品性能,促进冬枣产业发展具有重要意义。然而,针对冬枣施用钙肥效应的研究鲜见报道,尚未有研究对醋酸钙等有机钙肥的应用效果进行探讨。通过在果实白熟期对冬枣叶果喷钙,研究单喷施氯化钙、醋酸钙以及两者配合喷施对冬枣品质指标以及常温下贮藏性能的影响。结果表明,采前喷钙可显著提高冬枣可溶性固形物、可滴定酸、Vc、钙、总黄酮含量和果实硬度,改善了冬枣品质;与单喷施氯化钙相比,采前喷施醋酸钙可显著提高可溶性固形物、钙和总黄酮含量;氯化钙与醋酸钙配合喷施处理对枣果品质的改善效果与单施醋酸钙的效果相似。在常温贮藏条件下,采前喷施醋酸钙促进了冬枣可溶性固形物含量的升高,加速了酸度降低,减缓了Vc消耗,同时减轻了贮藏期间软化现象。与单喷施氯化钙相比,喷施醋酸钙处理更有利于冬枣保持水分,延长其货架期。综合分析认为,在冬枣白熟期喷施醋酸钙是改善果实品质和耐藏性能的有效措施。  相似文献   

大瑶山野生茶树的生化特性研究     

蒋陈凯  赵文芳  黄亚辉  曾贞  吴春兰  赖幸菲  袁思思 《茶叶科学技术》2017,58(1)

为探究大瑶山山脉野生茶树资源的生化成分特征,本研究测定了来自大瑶山29份茶树单株的19项生化指标.其中用分光光度法检测茶多酚、游离氨基酸、黄酮等含量,用高效液相色谱检测儿茶素、生物碱等组成,用氨基酸分析仪检测氨基酸组成.对主要生化成分进行遗传多样性分析和聚类分析,结果表明大瑶山野生茶具有丰富的遗传多样性和地域独特性,并从中筛选出了一批特异资源,如高氨基酸(＞4.00％)资源9份、高咖啡碱(＞5.00％)资源23份、高EGCG(＞10％)3份.  相似文献   

草地贪夜蛾对小麦和玉米的产卵选择性及其种群生命表     

巴吐西  张云慧  张智  关豆豆  李翠翠  季昭云  殷新田  张爱环  汤清波  刘延辉  李祥瑞  朱勋 《植物保护》2020,46(1):17-23

为明确草地贪夜蛾对小麦的产卵选择性及其是否对小麦安全生产构成威胁,本研究以玉米和小麦作为测试寄主,比较分析了草地贪夜蛾对两种作物不同部位的产卵选择性,并利用两性生命表方法研究了取食小麦、玉米对其生命参数的影响。结果表明:草地贪夜蛾更喜欢在玉米上产卵,其在玉米、小麦叶片、玉米和小麦茎秆上的产卵量存在显著差异(df=102,F=15.593,P<0.05),以玉米叶片背面卵块数量(7.11±1.55)块/笼最高;草地贪夜蛾取食小麦可以完成生活史,但幼虫存活率、化蛹率、羽化率和世代存活率低于取食玉米。取食玉米的幼虫发育历期为(16.31±0.15)d,显著高于取食小麦的(14.66±0.12)d,蛹期、蛹重、产卵前期、成虫寿命和世代周期无显著差异。取食小麦羽化出的雌虫寿命、平均单雌产卵量显著高于取食玉米,分别为(16.39±0.40)d、(976.31±57.21)粒和(14.64±0.32)d、(831.57±30.55)粒。生命表参数显示取食玉米的净增殖率为363.14,显著高于小麦的258.63,但内禀增长率、周限增长率和平均世代周期无显著差异。研究结果为草地贪夜蛾在小麦上的预测预报和有效防控提供了基础数据。  相似文献   

Effects of Artificial Warming from Late-Winter to Early-Spring on Photosynthesis and Flag Leaf Senescence of Winter Wheat     

YAN Peng  SUN XiaoNuo  DU Xiong  GAO Zhen  BIAN DaHong 《中国农业科学》2019,52(15):2581-2592

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不同秧龄对沿江地区双季晚稻秧苗素质及产量的影响   总被引：1，自引：0，他引：1  

潘志军  吴小文  陈刚  吴晨阳  张晓红  尹玲  吴文革  吕和平 《中国稻米》2017,(6):97-100

以早熟高产品种上农粳2号和镇稻18为材料,采用常规毯苗机械栽插,分别设置20 d、24 d、28 d、32 d等4个秧龄处理,研究不同秧龄对沿江地区双季晚稻秧苗素质、生育进程、茎蘖动态及产量构成的影响。结果表明,随秧龄天数增加,参试品种的绿叶数、茎基宽和根系盘结力均逐渐升高;与20 d、24 d和28 d秧龄的处理相比,32 d秧龄处理的成熟期提前4~5 d,茎蘖高峰期提早15 d左右,结实率显著升高;与24 d秧龄处理相比,上农粳2号和镇稻18在32 d秧龄处理下的产量分别提高10.26%和20.82%。在秧苗强化化控和苗床管理的条件下,安徽沿江地区常规毯苗机插的栽插秧龄可延长至32 d。  相似文献   

Effect of beclin-1 gene silencing on TuAKe-injured gastric cancer SGC-7901 cells     

PAN Lei  HU Meng-yi  LI Sai  CHEN Pei-feng  YIN Li-ming 《园艺学报》2019,35(3):436-441

AIM: To observe the effect of beclin-1 silencing by the technique of RNA interference on the injury of human gastric cancer SGC-7901 cell by Sheliugu extract (the extract from tuber of Amorphophallus konjac, TuAKe). METHODS: To knock down the expression of beclin-1 gene, SGC-7901 cells were transfected with lentiviral vector carrying beclin-1-shRNA. The beclin-1 gene knock-down and non-knock-down SGC-7901 cells were treated with TuAKe. The cell viability was analyzed by CKK-8 assay. The percentages of apoptotic cells were detected by flow cytometry. The expression of beclin-1 and LC3 was detected by Western blot. RESULTS: The beclin-1 gene silencing decreased the protein expression of beclin-1 and increased the protein expression of LC3 in the SGC-7901 cells, leading to the decrease in cell viability and the increase in apoptotic rate (P<0.05). TuAKe increased the protein expression of beclin-1 and LC3 in the SGC-7901 cells, and decreased the protein expression of LC3 in the SGC-7901 cells with beclin-1 gene silencing, thus inhibiting the cell viability and increasing the apoptotic rate (P<0.05). CONCLUSION: Beclin-1 gene silencing inhibits the activation of beclin-1-related signaling pathway in gastric cancer SGC-7901 cells, and aggravates the injury of cell viability induced by TuAKe.  相似文献   

Leaf carbohydrates assimilation and metabolism affect seed yield of rapeseed with different waterlogging tolerance under the interactive effects of nitrogen and waterlogging     

Jie Kuai  Xiaoyong Li  Zhen Li  Yan Xie  Bo Wang  Guangsheng Zhou 《Journal of Agronomy and Crop Science》2020,206(6):823-836

Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.  相似文献