Screening and Verification of Low Nitrogen Tolerant and Nitrogen Sensitive Cotton Germplasm     

Wang Zhun  Zhang Hengheng  Dong Qiang  Gui Huiping  Wang Xiangru  Pang Nianchang  Li Yongnian  Niu Jing  Jin Dingsha  Wang Sujie  Zhang Xiling  Song Meizhen 《棉花学报》2021,32(6):538-551

[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L^－1) and high (5 mmol·L^－1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient.  相似文献   

绿僵菌毒素的提取     

李农昌  樊美珍朱玮 柴长宏 《西北林学院学报》1989,4(1):69-71

用金龟子绿僵菌的液物培养体,提取绿僵菌素。每升培养液得绿僵菌素0.7mg。用绿僵菌素注射青杨天牛幼虫,每克虫体用量10.6μg,3d内死亡率达100％。  相似文献   

秀珍菇营养配方的试验   总被引：3，自引：0，他引：3  

劳有德  韦文添 《中国食用菌》2004,23(3):32-34

本研究通过三因素三水平的正交试验表明 ,在培养基中添加VB1(2× 10 -6) ,N P K (0 1% ) ,NAA(10× 10 -6) ,最适合秀珍菇菌丝的生长  相似文献   

农药除蜗灵对土壤微生物的影响   总被引：1，自引：0，他引：1  

赵琦  唐美珍 《绿色科技》2014,(10)

指出了农药除蜗灵近年来的大量使用,对土壤环境质量产生了一定的影响,进行了其对土壤-植物根际微生物数量上的影响的试验,并设计了农药浓度和施药时间的不同,从而了解了农药除蜗灵对微生物的长远影响。为农药除蜗灵的施用时间、施用浓度及其对土壤环境质量的影响提供了一定的科学依据。通过采用倾注法分离土壤中微生物,利用平板计数法进行计数,可得知农药除蜗灵对土壤中微生物的影响如下:对细菌和放线菌都产生了明显的促进作用,而对霉菌产生了显著的抑制作用。随着时间的推移,农药除蜗灵对放线菌的增加作用最先减弱,霉菌次之,细菌最晚恢复,这又说明了土壤本身对农药具有一定的净化作用。  相似文献   

青海省农作物品种管理数据库系统的建立及应用技术研究     

张有谊  罗新青  王志远  李俊仁  宋晓红 《种子》1996,(5)

青海省首次建成并使用的“青海省农作物品种管理数据库系统”是促进全省品种管理、合理推广应用优良品种，为社会提供品种咨询服务业务的信息系统工程，对发展农业生产，提高良种覆盖率具有很高的实用价值和理论意义。  相似文献   

Effect of nutrient solution salinity and ionic concentration on parsley (Petroselinum crispum Mill.) essential oil yield and content     

Juan Eugenio Álvaro  María Teresa Lao  Mourad Baghour  Moumen Abdelmajid 《Journal of plant nutrition》2016,39(8):1057-1062

The growth and essential oil (EO) production of parsley were evaluated in response to salinity and nutrient solution concentrations in a soilless culture. Parsley plants that were 60 days old were potted in a coconut fiber and peat moss medium and were treated with four different nutrient solutions, including T1, T2, T3 and T4. The T1 nutrient solution was the standard, the T2 and T3 solutions contained incremental macronutrient concentrations with an electrical conductivity (EC) of up to 2.2 and 3.2 dS m^?1, respectively, and the T4 solution was the same as T2 but with sodium chloride (NaCl) and an incremental macronutrient concentration with an EC of 3.2 dS m^?1. Next, these plants were grown for 90 days in a greenhouse with natural daylight in Nador, Morocco. Shoot and root growth significant decreased with increasing EC. However, the salinity that resulted from the addition of NaCl did not affect plant growth in the nutrient solutions. The optimum obtained growth and EO production were 1.2 and 2.2 dS m^?1, respectively. Consequently, the optimum EC value (based on the EO production) of parsley in the soilless culture was 1.2–2.2 dS m^?1.  相似文献   

两种低氧条件对赤拟谷盗不同虫态的抑制作用     

劳传忠  曾伶  郭超 《粮食储藏》2012,41(4):27-29

研究了在28℃、75%RH的温湿度条件下,5%和2%低氧储藏条件对赤拟谷盗高龄幼虫、蛹和成虫的抑制作用。在5%和2%氧气条件下暴露15d后,赤拟谷盗高龄幼虫化蛹率分别为95.0%和3.7%,蛹羽化率分别为98.0%和0%,成虫死亡率分别为12.7%和100%。本研究结果为气调储粮防治害虫提供依据。  相似文献   

先锋植物沙棘Ⅲ——生活史分析   总被引：1，自引：0，他引：1  

林美珍  张吉科  张国伟  张小民 《国际沙棘研究与开发》2006,4(4):1-7

就沙棘生态习性,应归属r-型生态对策型。沙棘的生活史具两型性(有性和无性繁殖),是对有限资源权衡利用与分配在生命维持-生长-繁殖-扩散上的不均衡结果,是长期自然选择,进化的结果。固氮与克隆生长有利与扩大了r-型生态对策。雌雄异株的进化,避免了近交衰退和提高了沙棘的适合度。沙棘的花序分化受多种同源异型基因调控,而性别决定则受Y性染色体上性基因连锁决定。沙棘繁殖力很高,但衰老较快,是一种生命期(20年左右)较短的灌木。  相似文献   

牧草收割机械的现状和发展趋势   总被引：4，自引：0，他引：4  

傅美贞 《农机化研究》2009,31(6)

针对浙江省牧草畜牧业和草坪种植业的发展,分析了国内外草业收割机械的现状以及浙江省发展牧草收割机械的机遇,并提出浙江省牧草收割机械应向价格低廉、与已普及的农机具配套使用、适合小田块和丘陵地区作业的牧草收割机械方向发展,认为研制一种既能适用于牧草收割与荒地杂草的整理又能适用于绿化草坪的维护和修剪的中小型割草机是十分必要的.  相似文献   

陆地棉转录因子GhNAC78基因的特征及功能分析     

赵凤利  范术丽  宋美珍  庞朝友  郭亚宁  李黎贝  魏恒玲  喻树迅 《棉花学报》2014,26(4):283-289

本实验室从陆地棉中克隆得到GhNAC78,分析了其基因特征及功能。该基因cDNA全长为937 bp,开放阅读框为876bp,编码291个氨基酸,隶属于NAM转录因子亚家族。体外转录激活实验表明,GhNAC78具有转录激活活性。上游启动子区1537 bp序列的生物信息学分析可知其包含多种激素、胁迫和光响应元件,表明GhNAC78广泛地参与植物生长发育和胁迫响应的调控。荧光定量结果显示,GhNAC78在棉花叶片衰老过程中高调表达,推测其参与叶片衰老的调控。  相似文献