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El Hassan Nouar Danny Vereecke Koen Goethals Mondher Jaziri Marie Baucher 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(4):327-330
To better characterise at the molecular level the nature of plant responses to infection by Rhodococcus fascians PCR-based differential display patterns of Atropa belladonna leafy gall (LG) and non-infected plant tissues were compared. Six differentially expressed genes were identified and their altered expression was confirmed by RT-PCR. Three of them corresponded to up-regulated genes which encode proteins involved in plant defence. The three remaining cDNA fragments which correspond to down-regulated genes in LG, encoded proteins with similarity to a multicystatin, a miraculin and a methallothionein-like protein, respectively. Upon elimination of the bacteria from infected plant tissue, the expression of up-regulated genes was maintained, whereas expression of down-regulated genes resumed suggesting a potential role of these up-regulated genes in plant growth and development. 相似文献
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Les variations pluriannuelles de la croissance végétative (orthotrope et plagiotrope) et de la production et du taux d'infestation des citrus par Tylenchulus semipenetrans suivent une évolution cyclique et des amplitudes décalées les unes par rapport aux autres. Ces variations présentent deux phases distinctes. La première se caractérise par une diminution de la population des nématodes dans les racines, une augmentation de la croissance plagiotrope aux dépens de la croissance orthotrope et une production annuelle stable. La deuxième phase se caractérise par contre par une augmentation de l'infestation, une croissance orthotrope plus dominante et une alternance annuelle de la production des agrumes. L'intervention chimique à l'aldicarbe permet de réduire l'incidence des nématodes sur les agrumes, mais seuls les traitements appliqués au cours de la deuxième phase sont efficaces contre les nématodes. 相似文献
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Randriamampionona D Diallo B Rakotoniriana F Rabemanantsoa C Cheuk K Corbisier AM Mahillon J Ratsimamanga S El Jaziri M 《Fitoterapia》2007,78(7-8):482-489
A comparative quantitative analysis of the active triterpenoids in Centella asiatica samples collected in different locations in Madagascar was carried out to evaluate the natural variability in triterpenoid content and to select elite samples for further ex situ germplasm conservation and clonal propagation. The highest asiaticoside content (6.42%) was measured in samples collected in Mangoro region. In vitro propagation of C. asiatica was successfully achieved in hormone-free medium. Although lower asiaticoside content was detected in 8-week-old vitro plants, the Mangoro sample still showed the highest content in this triterpenoid constituent (1.78%). Acetoxycentellynol, a C(15)-polyacetylene, was found to be accumulated up to 18 times more in in vitro plants as compared to plant material collected in situ. 相似文献
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Lucchetta L Manriquez D El-Sharkawy I Flores FB Sanchez-Bel P Zouine M Ginies C Bouzayen M Rombaldi C Pech JC Latché A 《Journal of agricultural and food chemistry》2007,55(13):5213-5220
Alcohol acyltransferases (AAT) play a key role in the biosynthesis of ester aroma volatiles in fruit. Three ripening-specific recombinant AATs of cantaloupe Charentais melon fruit (Cm-AAT1, Cm-AAT3, and Cm-AAT4) are capable of synthesizing thioether esters with Cm-AAT1 being by far the most active. All proteins, as well as AAT(s) extracted from melon fruit, are active as tetramers of around 200 kDa. Kinetic analysis demonstrated that CoA-SH, a product of the reaction, is an activator at low concentrations and an inhibitor at higher concentrations. This was confirmed by the addition of phosphotransacetylase at various concentrations, capable of modulating the level of CoA-SH in the reaction medium. Site-directed mutagenesis of some amino acids that were specific to the Cm-AAT sequences into amino acids that were consensus to other characterized AATs greatly affected the selectivity of the original protein and the number of esters produced. 相似文献
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病毒诱导的基因沉默（VIGS）是植物基因功能研究的新技术。以组成型表达gusA基因的转基因番茄为材料,通过VIGS技术沉默gusA基因,对VIGS在番茄中的组织特异性进行了初步分析。结果表明,VIGS技术能有效地诱导gusA基因在番茄植株的叶片、花、果实、侧枝、主茎及茎分枝点等部位和组织中沉默,证明VIGS在番茄中具有较强的系统性沉默特征。 相似文献
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Yulien Miguelez-Sierra Annia Hernández-Rodríguez Yanelis Acebo-Guerrero Marie Baucher Mondher El Jaziri 《The Journal of Horticultural Science and Biotechnology》2017,92(1):25-30
This study aimed to evaluate in vitro grafting of Theobroma cacao where seedlings of the UF 677 genotype were used as the rootstock and apices or axillary buds of a Trinitarian genotype were used as scion. Three methods of grafting using scions from seedlings were evaluated. Apical grafts using apex and side grafts using apex displayed better graft success (95 and 80%, respectively). However, side grafts using axillary buds reached a greater height on average and a higher number of leaves per plant (1.76 cm and 3.72, respectively). Histological studies revealed new vascular elements at the graft union area. Side grafts with axillary buds provided the highest survival rate (82%) after the acclimatization step. A shoot of at least 1 cm with two leaves is required for plant survival after transfer to ex vitro conditions. Side grafting was carried out with axillary buds from adult trees and nursery plants. Only the grafts with buds from nursery grafted plants were successful, with a rate of 26%. Overall, side grafting with axillary buds is the most appropriate method for cacao micrografting. This method can be used for clonal propagation and for the establishment of in vivo and/or in vitro cacao germplasm collection. 相似文献
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