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1.
Landscape Ecology - Human activities have led to the degradation of wetlands, impinging on their capacity to deliver essential ecosystem services to society. Wetland restoration now appears an...  相似文献   
2.
In forest soils where a large fraction of total phosphorus (P) is in organic forms, soil micro-organisms play a major role in the P cycle and plant availability since they mediate organic P transformations. However, the correct assessment of organic P mineralization is usually a challenging task because mineralized P is rapidly sorbed and most mineralization fluxes are very weak. The objectives of the present work were to quantify in five forest Spodosols at soil depths of 0-15 cm net mineralization of total organic P and the resulting increase in plant available inorganic P and to verify whether net or gross P mineralization could be estimated using the C or N mineralization rates. Net mineralization of total organic P was derived from the net changes in microbial P and gross mineralization of P in dead soil organic matter. We studied very low P-sorbing soils enabling us to use lower extractants to assess the change in total inorganic P as a result of gross mineralization of P in dead soil organic matter. In addition, to enable detection of gross mineralization of P in dead soil organic matter, a long-term incubation (517 days) experiment was carried out. At the beginning of the experiment, total P contents of the soils were very low (19-51 μg g−1) and were essentially present as organic P (17-44 μg g−1, 85-91%) or microbial P (6-14 μg g−1; 24-39%). Conversely, the initial contents of inorganic P were low (2-7 μg g−1; 9-15%). The net changes in the pool size of microbial P during the 517 days of incubation (4-8 μg g−1) and the amounts of P resulting from gross mineralization of dead soil organic matter (0.001-0.018 μg g−1 day−1; 0.4-9.5 μg g−1 for the entire incubation period) were considerable compared to the initial amounts of organic P and also when compared to the initial diffusive iP fraction (<0.3 μg g−1). Diffusive iP corresponds to the phosphate ions that can be transferred from the solid constituents to the soil solution under a gradient of concentration. Net mineralization of organic P induced an important increase in iP in soil solution (0.6-10 μg g−1; 600-5000% increase) and lower increases in diffusive iP fractions (0.3-5 μg g−1; 300-2000% increase), soil solid constituents having an extremely low reactivity relative to iP. Therefore, soil micro-organisms and organic P transformations play a major role in the bioavailability of P in these forest soils. In our study, the dead soil organic matter was defined as a recalcitrant organic fraction. Probably because gross mineralization of P from this recalcitrant organic fraction was mainly driven by the micro-organisms’ needs for energy, the rates of gross mineralization of C, N and P in the recalcitrant organic fraction were similar. Indirect estimation of gross mineralization of P in dead soil organic matter using the gross C mineralization rate seems thus an alternative method for the studied soils. However, additional studies are needed to verify this alternative method in other soils. No relationships were found between microbial P release and microbial C and N releases.  相似文献   
3.
The purpose of this study was to evaluate the multiple dose pharmacokinetics and acute safety of piroxicam and cimetidine alone and in combination in cats. Seven healthy cats were included in this randomized-crossover study. The cats were assigned to groups designated to receive cimetidine alone (15 mg/kg, p.o., q12 h), piroxicam alone (0.3 mg/kg, p.o., q24 h), and piroxicam combined with cimetidine (both at aforementioned doses). The cats were dosed for 10 days followed by at least a 2-week washout period between trials. Serial blood samples were collected following the first and last doses and analyzed utilizing a high-performance liquid chromatography with mass spectrometry detection (LC/MS) assay. Pharmacokinetic parameters were determined using noncompartmental analysis. Endoscopic evaluation of the gastric mucosa was performed and serum urea nitrogen (SUN), creatinine, alkaline phosphatase (ALP), and alanine transaminase (ALT) activities were evaluated. There were not a clinically relevant difference between the pharmacokinetic parameters of piroxicam administered alone or in combination with cimetidine after either the first or last dose. Gastric ulcers were not observed in any cats although gastric erosions were. The SUN, creatinine, ALP, and ALT activities remained within reference ranges for all cats. It appears that once daily, short-term use of piroxicam alone and in combination with cimetidine in cats is relatively safe based on the parameters evaluated in this study. However, further studies are necessary to determine the long-term gastrointestinal safety of piroxicam.  相似文献   
4.
The anthocyanin composition of Syrah grapes harvested at different stages of ripening and produced using organic or conventional agriculture was studied. Samples of grapes were collected from veraison to full maturity in each plot, and the content in nine anthocyanins was determined by high-performance liquid chromatography with diode array detection. The total content in anthocyanins during ripening of the conventionally grown grapes was significantly higher compared to that found in the organic production. The accumulation of anthocyanins reached a maximum 28 days after veraison (in agreement with high temperature) and then decreased until harvest. In all samples, grapes from the conventional agriculture presented higher proportions of delphinidin, petunidin, malvidin, and acylated malvidin glucosides compared to grapes from organic agriculture. In contrast with other comparative studies of organically and conventionally grown plants, the results demonstrated a higher content in anthocyanins in conventionally grown grapes.  相似文献   
5.
A 3-year field experiment with paired exclosure (fenced areas, excluding deer) and control plots (unfenced areas, free access to deer), with two treatments with and without woody debris, was carried out at two sites in a temperate forest in eastern France. The aim of the experiment was to assess the effect of browsing by roe deer (Capreolus capreolus) and red deer (Cervus elaphus) on the diversity and richness of plant species and to test the effectiveness of using woody debris to protect seedlings and saplings from deer browsing. In presence of deer, both plant species richness and diversity were reduced the first year of the study, but this negative impact of deer then disappeared after 3 years. Deer browsing mostly affected species composition of plant communities. We observed a decrease in the abundance of preferred species such as Carpinus betulus, Rubus fructicosus, Rubus idaeus, Anemone nemorosa and Epilobium angustifolium, and palatable species such as Acer spp., Carex spp., Festuca spp. and Mycelis muralis, whereas unpalatable species such as Lamium spp., or species particularly resilient to browsing such as grasses (Brachypodium spp. and Luzula spp.) increased in abundance. The use of woody debris as protection against browsing by deer did not limit damage to seedlings and saplings of the main commercially valuable species, Abies alba and Quercus spp. Instead of limiting deer impact, use of woody debris seemed to increase the negative effect of deer browsing on regeneration in control plots relatively to those without protection.  相似文献   
6.
Phosphorus (P) is the least mobile nutrient in the soil as compared to other macronutrients and therefore frequently limits crop growth. During germination and early growth, seed‐phytate hydrolysis and seed‐P remobilization is the major P source for developing seedlings. The objective of this paper was to investigate whether seed‐P hydrolysis and remobilization of nonphytate P are sufficient for seedling P nutrition during early growth stages of maize. A large part of initial maize endogenous seed P reserves are mainly in the form of phytate. Till 70 cumulated degree days after sowing, nearly all the phytate (98%) was hydrolyzed and caused an increase in nonphytate P in seeds. Phytate hydrolysis and remobilization of nonphytate P was the main source of P supply for the newly growing seedlings and was not a limiting step for seedling P nutrition during the first four weeks of early growth.  相似文献   
7.
This study aimed at assessing the pathogenicity of two Vibrio splendidus-related species and evaluating the influence of the origin and annual life cycle of mussels on their sensitivity during a bacterial challenge. Thus, in vivo infection assays were made with Vibrio crassostreae 7T4_12 and Vibrio splendidus 3G1_6, over, respectively, thirteen and 9 months, on adult blue mussels from five recruitment areas in France. Two bacterial concentrations were tested: one consistent with the loads of Vibrio spp. in environment and mussel tissues (~105 CFU/ml) and another one much higher (~108 CFU/ml). The tested environmental concentration has no pathogenic effect whatever the time of year, the strain used and the origin of mussels. However, at the highest concentration, a pathogenic effect was observed only at specific moments, and one of the origins appeared to be more resistant. The physiological state of mussels—depending on the time of year—seemed significant in mussels’ sensitivity, as their recruitment origin. This study is the first to test the pathogenicity of V. splendidus-related strains at concentrations close to what is found in the wild, over the annual cycle of mussels, and considering their origin.  相似文献   
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9.
The aim of this study was to compare the influence of a high α-linolenic acid (ALA) diet (linseed diet) and a high linoleic acid (LA) diet (sunflower diet) on performances of pigs, on the dietetical quality of their tissues (adipose tissues and muscles), and on the lipogenic potential of these tissues. Growth and carcass performances, and the lipid content of the tissues were not affected by the diet. Feeding the linseed diet increased the content of n-3 PUFA and decreased the LA/ALA ratios in all the tissues, while the sunflower diet led to an increase in the n-6 PUFA contents. Neither the stearoyl-CoA-desaturase and glucose-6-phosphate-dehydrogenase activities nor the β-hydroxyacyl-CoA-dehydrogenase activity were affected by the diet. Acetyl-CoA-carboxylase and fatty acid synthase activities were enhanced with the linseed diet in respectively subcutaneous adipose tissue and intermuscular adipose tissue, while malic enzyme activity was decreased in liver and subcutaneous adipose tissue of pigs fed the linseed diet.  相似文献   
10.
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