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New Forests - Adventitious rooting (AR) is an obligatory step for vegetative propagation of commercial woody species. Paper industries have interest in Eucalyptus globulus Labill and its hybrids...  相似文献   
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We characterized the adventitious rooting response of Eucalyptus globulus Labill. to various concentrations of calcium, nitrogen, phosphorus, iron, manganese, zinc, boron and copper. The parameters analyzed were percent rooting, root number, root length and mean rooting time. Root number and root length were significantly affected by mineral nutrition, whereas mean rooting time and rooting percentage seemed to be closely related to auxin availability. Root number was affected by calcium, nitrogen source and zinc, whereas root length was influenced by concentrations of phosphorus, iron and manganese, and by nitrogen source. Based on these results, we evaluated various combinations of several concentrations of these minerals in each rooting phase. Cuttings that were rooted in an optimized mineral nutrient medium and acclimatized to ex-vitro conditions for two months showed significantly higher survival after transplanting and drought stress than cuttings rooted in basal medium and treated in the same way.  相似文献   
3.
The influence of epicatechin (EC) on off-flavor development in low-heat skim milk powder samples during processing and storage was investigated. Milk powder samples were prepared from a concentrated skim milk (control) plus a concentrated skim milk with EC (treatment). Volatile extracts of the powders were analyzed by aroma extract dilution analysis (AEDA) at 0 days and after 17 months of storage in conjunction with sensory analysis of the flavor attributes. The treatment milk powders with EC added prior to drying reported a reduction in the formation of three main compounds, 4-hydroxy-2,5-dimethyl-3-(2H)-furanone, o-aminoacetophenone, and furfural, by 8-, 4-, and 4-fold for the 0 day old samples, while for the 17 month aged samples o-aminoacetophenone was the major compound reduced in formation by 8-fold, respectively, based on the flavor dilution factors reported. The sensory evaluations indicated that the treatment milk powders for 0 day old and 17 month aged samples were statistically lower (alpha = 0.05) in stale flavor intensity in comparison to the respective control samples, while no differences were noted in bitterness intensity.  相似文献   
4.
Ripe rot is one of the most important bunch diseases of grapes in the highlands of southern Brazil and a matter of concern for winegrowers. Sixty-one strains isolated from berries of Vitis labrusca and V. vinifera varieties with symptoms were classified by phylogenetic analysis of the sequences of β-tubulin, glyceraldehyde-3-phosphate dehydrogenase, D1/D2 domain of 28S rDNA, and rDNA internal transcribed spacer. They were also characterized by morphology, and their pathogenicity was evaluated. The combined molecular data allowed identification of six Colletotrichum species: C. fructicola, C. kahawae and C. viniferum (gloeosporioides complex), C. limitticola, C. nymphaeae (acutatum complex), and C. karstii (boninense complex). This is the first report of C. karstii and C. limitticola associated with the ripe rot of grapes. Morphological characteristics varied within and among species, confirming their separation at the complex level. Pathogenicity tests on V. vinifera berries showed that the most prevalent species, C. viniferum (37.8%) and C. fructicola (36.1%), were more virulent than the less prevalent species C. limitticola and C. karstii. Our findings indicate that there is a high diversity of Colletotrichum species associated with ripe rot disease of grapes in Brazil. There were no clear differences in the distribution of Colletotrichum species between V. labrusca and V. vinifera varieties. The determination of fungal species responsible for grape ripe rot in Brazilian vineyards may contribute to further epidemiological studies and the development of more efficient prophylactic methods for ripe rot management.  相似文献   
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Eucalyptus globulus Labill and hybrids thereof have low lignin content, favoring cellulose extraction, but are often recalcitrant to clonal propagation. This work analyzed biochemical and morphological changes during adventitious rooting of mini-cuttings of E. globulus × maidenni obtained from mini-stumps cultured in drip fertigated sand bed or intermittent flooding tray commercial propagation systems. Morphological (% rooting, root number and length, mean rooting time) and biochemical parameters (peroxidase activity, total phenolic content and flavonoid content) were monitored to characterize the rooting phases. All of the rooting parameters were equivalent in both systems, indicating comparable efficiency of both methods in clonal propagation. Kinetic profiles of biochemical parameters were also similar, although the activity of peroxidases was an order of magnitude higher and the phenolic content about three times lower in cuttings derived from intermittent flooding-grown mini-stumps than in those derived from sand bed-grown mini-stumps. Taken together, results suggest that rooting phases were similar in both systems: induction before day 5, formation from day 5 to 15, and elongation from day 15 to 45. These data may contribute to the development of rooting phase-specific mineral nutrient solutions to maximize clonal propagation and plant survival.  相似文献   
6.
In previous studies, it has been shown that nitrate supply may favor adventitious rooting in the rooting recalcitrant Eucalyptus globulus. Herein, the impact of various N sources on adventitious rooting and root branching in microcuttings of E. globulus was investigated. The positive effect of nitrate on adventitious root development was confirmed and extended to root branching. Urea yielded a rooting response comparable to that observed in presence of nitrate. Urease activity was observed, displaying two peaks: one at the root induction and another at the root formation step. The use of glutamic acid, glutamine or asparagine promoted higher root number, but yielded shorter roots. Rooted microcuttings derived from all nitrogen (N) sources were successfully acclimated to ex vitro conditions. The manipulation of N sources in adventitious rooting media can be a tool for improving new root density, length and branching in this species.  相似文献   
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