Water availability affects nectar sugar production and insect visitation of the cup plant Silphium perfoliatum L. (Asteraceae)     

Anna Lena Mueller  Christian A. Berger  Siegfried Schittenhelm  Burkhard Stever-Schoo  Jens Dauber 《Journal of Agronomy and Crop Science》2020,206(5):529-537

The perennial cup plant (Silphium perfoliatum L.) is considered as an alternative feedstock to maize for biogas plants. Due to its ecological advantages of an extensive management and function as food resource for pollinators, it can be grown in Ecological Focus Areas (EFAs) since 2018. However, studies at the Julius Kühn-Institute in Braunschweig (Germany) showed that the assumed advantage of the cup plant of a high drought tolerance could not be confirmed regarding aspects of crop production and yield. We complemented this experiment by assessing how different soil moisture conditions affect the production of floral resources and insect visitation. In 2014, we sampled three irrigated and three rainfed plots of the cup plant. We quantified the nectar volume and sugar mass per inflorescence, the number of inflorescences per plant and calculated the total nectar sugar production. We further counted insect visitation on the inflorescences. Due to reduced numbers of inflorescences per plant and an earlier harvest, the estimated nectar sugar production was 58 kg/ha regarding irrigated and 20 kg/ha regarding rainfed plots. Honeybee visitation per inflorescence was about twice as high in the irrigated plots. Furthermore, the early harvest is a disadvantage for wild pollinators with a late activity period.  相似文献   

Metabolism of the pesticide metabolites 4-nitrophenol and 3,4-dichloroaniline in carrot (Daucus carota) cell suspension cultures     

Burkhard Schmidt  Brigitte Thiede  Christiane Rivero 《Pest management science》1994,40(3):231-238

The metabolism of [ 14 C]-4-nitrophenol and [ 14 C]-3,4-dichloroaniline (the xenobiotics are degradation products of parathion and propanil, respectively) was studied in cell suspension cultures of carrot (Daucus carota L.). 4-Nitrophenol was transformed almost quantitatively to water-soluble conjugates with minor amounts of non-extractable residues. The conjugates identified were 1-(O-β-D-glucopyranosyl)-4-nitrobenzene and 1-(6′-O-malonyl-O-β-D-glucopyranosyl)-4-nitrobenzene. In addition, two unidentified metabolites were observed, possibly a disaccharide and another malonylated glycoside of 4-nitrophenol. Time-course studies demonstrated that 4-nitrophenol was rapidly taken up and conjugated; all metabolites remained associated with the cells rather than nutrient medium. 3,4-Dichloroaniline was transformed quantitatively to water-soluble conjugates and bound residues (3.6%). The water-soluble metabolites were identified as 6′-O-malonyl-N-(β-D-glucopyranosyl)-3,4-dichloroaniline, N-(β-D-glucopyranosyl)-3,4-dichloroaniline and N-malonyl-3,4-dichloroaniline. A time-course study showed that the glucosides were formed initially, then decreased, possibly due to hydrolysis. This decrease was paralleled by an increase of the main metabolite, N-malonyl-3,4-dichloroaniline, which was predominantly recovered from the medium.  相似文献   

Metabolism of [ring-2,6-14]parathion in plant cell suspension cultures of carrot (Daucus carota), purple foxglove (Digitalis purpurea), soybean,thorn apple (Datura stramonium) and wheat (Triticum aestivum)     

Doris Van Der Krol  Ingolf Schuphan  Brigitte Thiede  Burkhard Schmidt 《Pest management science》1995,45(2):143-152

By means of standardized procedures, the metabolism of [ring-2,6-¹⁴C]-parathion was investigated in carrot (Daucus carota L.), purple foxglove (Digitalis purpurea L.), soybean (Glycine max Merrill cv. ?Mandarin’?, and Glycine max Merrill cv. ?Harosoy 63’? cultivated on B5 and Miller media, respectively), thorn apple (Datura stramonium L.), and wheat (Triticum aestivum L.) cell suspension cultures. In the wheat and soybean (Mandarin) cells only 2.9 and 8.9%, respectively, of the applied parthion remained unmetabolized after 48 h of incubation, while 51.2, 57.9, 60.3, and 62.4% of the unchanged parent were detected in the D. purpurea, D. Stramonium, carrot and soybean (Harosoy) cultures, respectively. In all suspensions, paraoxon and 4-nitrophenol were found as phase I metabolites, thus demonstrating that plant tissues can catalyse oxidative desulfuration and dearylation of parathion. 4-Nitrophenol was also glycosylated with glucose and possibly galactose. Further, as yet unidentified, metabolites indicated that bio-transformations had also occurred at the aromatic moiety. Large amounts of non-extractable residues were detected in the wheat suspension (38.3%), while the other cultures showed a lower incorporation of ¹⁴C into insoluble cell material (0.9-9.4%). For a prospective ecotoxicological evaluation of the metabolic fate of pesticides and xenobiotics in plants in general, the differential metabolic capacity of plant cell cultures and plants should be taken into account.  相似文献   

Monoclonal Gammopathy in a Dog With Chronic Pyoderma     

Mary Jo Burkhard  Dennis J. Meyer  Rod A. Rosychuk  Shawn P. O'Neil  Patricia C. Schultheiss 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1995,9(5):357-360

A monoclonal gammopathy composed of immunoglobulin G, with concurrent light-chain proteinuria and generalized lymph node plasmacytosis, was associated with chronic pyoderma in a dog. A uniform population of plasma cells was observed cytologically and histologically in multiple lymph node specimens. A diagnosis of monoclonal gammopathy of unknown significance was eventually made by exclusion of other known causes of monoclonal gammopathy, resolution after antibiotic therapy, and no evidence of lymphoproliferative disease after 11 months of follow-up and subsequent necropsy. This report expands the diagnostic considerations for monoclonal gammopathies in the dog.  相似文献   

Einfluß steigender Stickstoffgaben auf den Kupferernährungszustand von Getreide     

Reiner Krhmer  Burkhard Sattelmacher 《植物养料与土壤学杂志》1997,160(3):385-392

Effect of increasing nitrogen fertilization on copper demand in cereals Pot experiments were conducted with wheat (2 experiments) and spring barley (1 experiment) in a peat soil. Four N and four Cu fertilizer levels were chosen in such a way that, based on grain yield, within each N level there was a Cu deficient and a Cu sufficient treatment. For sufficient Cu supply, Cu level in soil and plant increased with increasing N level. Critical Cu concentrations in aboveground biomass at shooting [μg (g dw)^?1] were 3.5 (lowest N level = N₁) to 7.5 (highest N level = N₄) for grain production of wheat and barley, but for aboveground biomass of wheat at shooting they were only 2.6 (N₁) to 5.8 (N₄). Based on critical Cu concentration at different N levels, critical Cu/N ratios were determined. These were independent from N level, indicating that Cu remobilization in the older leaves is unlikely even at N deficiency. The high critical Cu concentrations at high N levels may be explained by an increased immobilization of Cu. Under Cu deficient conditions, Cu concentration did not increase with increasing N level. However, under Cu sufficient conditions, N concentration and Cu concentration of the plant increased with increasing N level. The increasing Cu uptake with increasing N level may be explained by the release of amines into the root apoplast and the rhizosphere. Particularly under Cu sufficient conditions, these may mobilize Cu by formation of soluble Cu-amine complexes.  相似文献   

A well-preserved Archaeopteryx specimen with theropod features     

Mayr G  Pohl B  Peters DS 《Science (New York, N.Y.)》2005,310(5753):1483-1486

A nearly complete skeleton of Archaeopteryx with excellent bone preservation shows that the osteology of the urvogel is similar to that of nonavian theropod dinosaurs. The new specimen confirms the presence of a hyperextendible second toe as in dromaeosaurs and troodontids. Archaeopteryx had a plesiomorphic tetraradiate palatine bone and no fully reversed first toe. These observations provide further evidence for the theropod ancestry of birds. In addition, the presence of a hyperextendible second toe blurs the distinction of archaeopterygids from basal deinonychosaurs (troodontids and dromaeosaurs) and challenges the monophyly of Aves.  相似文献   

Retention of xenon in quartz and Earth's missing xenon     

Sanloup C  Schmidt BC  Chamorro Perez EM  Jambon A  Gregoryanz E  Mezouar M 《Science (New York, N.Y.)》2005,310(5751):1174-1177

The reactivity of xenon with terrestrial oxides was investigated by in situ synchrotron x-ray diffraction. At high temperature (T > 500 kelvin), some silicon was reduced, and the pressure stability of quartz was expanded, attesting to the substitution of some xenon for silicon. When the quartz was quenched, xenon diffused out and only a few weight percent remained trapped in samples. These results show that xenon can be covalently bonded to oxygen in quartz in the lower continental crust, providing an answer to the missing xenon problem; synthesis paths of rare gas compounds are also opened.  相似文献   

Morphology and Physiology of the Seminal Root System of Young Maize (Zea mays L.) Plants as Influenced by a Locally Restricted Nitrate Supply     

Burkhard Sattelmacher  Klaus Thoms 《植物养料与土壤学杂志》1995,158(5):493-497

In water culture the effect of a locally restricted NO₃ supply to the seminal root system of maize seedlings was studied. For this purpose plants were cultivated in containers with a wide-bore polyethylene tube positioned horizontally. Roots were suited through small holes in this tube and after sealing the holes with a non-toxic silicon putty, root segments in the tube could be exposed to a different nutrient solution from the rest of the root system in the outer compartment. In case of a locally restricted NO₃ supply (NO₃ was just supplied to the root segment within the tube), we observed an increase in root growth beginning at the fifth day after onset of the treatment. NO₃ uptake rate (¹⁵N) within the tube was significantly higher than in control plants (receiving NO₃ to the entire root system) as early as two days after onset of the treatment. One day later respiration (O₂ consumption) of the root segment exposed to NO₃ increased and at the same day we observed an accumulation of ¹⁴C activity (after pulse labelling of the shoots with ¹⁴CO₂) suggesting an increased phloem unloading. It is argued that this leads to the measured increase in IAA activity (Radio Immuno Assay) in the zone of NO₃ supply. Beginning at the fifth day we observed a stimulation of cell division rate (incorporation of ³H-methylthymidine), accompanied by an increase in length of first order lateral roots.  相似文献   

Chemical hydrolysis of 2-chloro-4,6-bis(alkylamino)-1,3,5-triazine herbicides and their breakdown in soil under the influence of adsorption     

Niklaus Burkhard  Johann A. Guth 《Pest management science》1981,12(1):45-52

The determination of rate constants and the calculation of the activation parameters [activation energy (E_a), free energy of activation(ΔG^≠)and entropy of activation (ΔS^≠)] demonstrated the identity of the reaction kinetics of chemical hydrolysis of the chlorinated triazine herbicides simazine, atrazine, propazine and terbuthylazine. Persistence in soil could be estimated, from the hydrolytic half-life time, only in pH regions where these compounds were also sensitive to chemical hydrolysis. In general, the rate of hydrolysis increased in the presence of soil as the result of a catalysing effect of the soil in their breakdown. When half-lives in soil of these triazine herbicides were compared with adsorption constants, a functional relationship was observed in both soil types; as adsorption increased the half-life in soil also increased.  相似文献   

Characterization of the bound residues of the fungicide cyprodinil formed in plant cell suspension cultures of wheat     

Sapp M  Ertunç T  Bringmann I  Schäffer A  Schmidt B 《Pest management science》2004,60(1):65-74

The non-extractable residues of the fungicide cyprodinil formed in heterotrophic cell suspension cultures of wheat were studied by application of [2-pyrimidyl-14C] or [2-pyrimidyl-13C]cyprodinil. The main objective was to examine whether solid-state and liquid 13C NMR spectroscopy can be used to examine plant bound residues of pesticides. For 14C experiments, wheat suspensions grown on glucose as carbon source were treated with 10 mg litre(-1) of 14C-cyprodinil. After incubation for 12 days, 20% of applied 14C was detected as non-extractable residues. The cell debris were treated with 0.1 M HCl (reflux), 1.0 M HCl (reflux), buffer, or 2 M NaOH (50 degrees C); Bj?rkman lignin and acidolysis lignin fractions were also prepared from the debris. Radioactivity liberated and solubilized by these procedures was examined by thin-layer chromatography and high-performance liquid chromatography. The results showed that cyprodinil and primary metabolites contributed to the fungicide's bound residues. Most of the residues (12% of applied 14C) remained associated with polar or polymeric/oligomeric endogenous cell materials in a stable manner. For the study with 13C-cyprodinil, wheat suspensions were cultivated on 13C-depleted glucose for four growth cycles, resulting in maximum 13C depletion of the natural cell components to about 0.10%. During the fourth cycle, 13C-labelled cyprodinil was applied, and cells were incubated (12 days). Cell debris was prepared and examined by solid-state 13C NMR spectroscopy. Debris was then treated as described above in the 14C experiment. Solubilized fractions were analyzed by liquid 13C NMR spectroscopy. However, none of the 13C NMR spectra recorded gave utilizable or unambiguous results, and all exhibited large inconsistencies, especially concerning the data from the conventional 14C experiment.  相似文献