Comparison of Antral and Preantral Ovarian Follicle Populations Between Bos indicus and Bos indicus‐taurus Cows with High or Low Antral Follicles Counts     

KC Silva‐Santos  LS Siloto  GMG Santos  F Morotti  TN Marcantonio  MM Seneda 《Reproduction in domestic animals》2014,49(1):48-51

The objective was to compare populations of antral and pre‐antral ovarian follicles in Bos indicus and Bos indicus‐taurus cows with high and low antral follicle counts. Nelore (Bos indicus, n = 20) and Nelore X Angus (1/2 Bos indicus‐taurus, n = 20) cows were subjected to follicular aspiration without regard to the stage of their oestrous cycle (day of aspiration = D0) to remove all follicles ≥3 mm and induce growth of a new follicular wave. Ovaries were examined by ultrasonography on D4, D19, D34, D49 and D64, and antral follicles ≥3 mm were counted. Thereafter, cows were assigned to one of two groups: high or low antral follicular count (AFC, ≥30 and ≤15 antral follicles, respectively). After D64, ovaries were collected after slaughter and processed for histological evaluation. There was high repeatability in the numbers of antral follicles for all groups (range 0.77–0.96). The mean (±SD) numbers of antral follicles were 35 ± 9 (Bos indicus) and 38 ± 6 (Bos indicus‐taurus) for the high AFC group and 10 ± 3 (Bos indicus) and 12 ± 2 (Bos indicus‐taurus) follicles for the low AFC. The mean number of preantral follicles in the ovaries of Bos indicus‐taurus cows with high AFC (116 226 ± 83 156 follicles) was greater (p < 0.05) than that of Bos indicus cows (63 032 ± 58 705 follicles) with high AFC. However, there was no significant correlation between numbers of antral and preantral follicles.  相似文献   

MALDI‐MS Lipid Profiles of Oocytes Recovered by Ovum Pickup from Bos indicus and 1/2 indicus × taurus with High vs Low Oocyte Yields     

KC Silva‐Santos  CR Ferreira  GMG Santos  MN Eberlin  LS Siloto  CO Rosa  TN Marcantonio  MM Seneda 《Reproduction in domestic animals》2014,49(5):711-718

The aim of the present study was to compare the lipid profile in oocytes of indicus and 1/2 indicus × taurus cows with high and low antral follicle count (AFC)/oocyte yields. After an OPU procedure (D0), antral follicles ≥3 mm were counted by ultrasonography (D4, 19, 34, 49, 64), and cows were assigned to groups with either high AFC (≥30 follicles; indicus, NH group; 1/2 indicus × taurus, AH group) or low AFC (≤15 antral follicles; indicus, NL group; 1/2 indicus × taurus, AL group). The lipid profiles of the oocytes were determined by MALDI‐MS. For GI, GII and GIII oocytes, the indicus samples tend to cluster separately from the 1/2 indicus × taurus samples. The lipid species [PC (P‐38:5) + H]⁺ and/or [PC (P‐36:2) + Na]⁺, [PC (38:2) + H]⁺, [PC (38:5) + Na]⁺ and [TAG (60:8) + NH⁴]⁺ were more abundant in indicus (NH and NL groups) than 1/2 indicus × taurus. The higher lipid content in the indicus oocytes likely reflects differences in the rate of lipid metabolism and may contribute to oocyte competence and embryo development.  相似文献   

Antioxidative compounds from the outer scales of onion   总被引：2，自引：0，他引：2  

Ly TN  Hazama C  Shimoyamada M  Ando H  Kato K  Yamauchi R 《Journal of agricultural and food chemistry》2005,53(21):8183-8189

Antioxidative compounds were isolated from the methanol extract of dry outer scales of onion (Allium cepa L.). Nine phenolic compounds (1-9) were finally obtained by reversed-phase high-performance liquid chromatography, and their structures were elucidated by NMR and mass spectrometry analyses. They were the six known compounds, protocatechuic acid (1), 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (2), quercetin 4'-O-beta-D-glucopyranoside (3), quercetin (5), 4'-O-beta-d-glucopyranoside of quercetin dimer (7), and quercetin dimer (8), and three novel compounds, condensation products of quercetin with protocatechuic acid (4), adduct of quercetin with quercetin 4'-O-beta-D-glucopyranoside (6), and quercetin trimer (9). These phenolic compounds were tested for their antioxidant properties using autoxidation of methyl linoleate in bulk phase or free radical initiated peroxidation of soybean phosphatidylcholine in liposomes. The flavonoid compounds having o-dihydroxy substituent in the B-ring were shown to be effective antioxidants against nonenzymic lipid peroxidation.  相似文献   

Isolation and characterization of some antioxidative compounds from the rhizomes of smaller galanga (Alpinia officinarum Hance)   总被引：2，自引：0，他引：2  

Ly TN  Shimoyamada M  Kato K  Yamauchi R 《Journal of agricultural and food chemistry》2003,51(17):4924-4929

Antioxidative compounds were isolated from the methanol extract of fresh rhizome of smaller galanga (Alpinia officinarum Hance). Seven phenylpropanoids (1-7) were finally obtained by reversed-phase HPLC, and their structures were elucidated by MS and NMR analyses. They comprised the two known compounds, (E)-p-coumaryl alcohol gamma-O-methyl ether (1) and (E)-p-coumaryl alcohol (6), and the five novel compounds, stereoisomers of (4E)-1,5-bis(4-hydroxyphenyl)-1-methoxy-2-(methoxymethyl)-4-pentene (2a and 2b), stereoisomers of (4E)-1,5-bis(4-hydroxyphenyl)-1-ethoxy-2-(methoxymethyl)-4-pentene (3a and 3b), (4E)-1,5-bis(4-hydroxyphenyl)-1-[(2E)-3-(4-acetoxyphenyl)-2-propenoxy]-2-(methoxymethyl)-4-pentene (4), (4E)-1,5-bis(4-hydroxyphenyl)-2-(methoxymethyl)-4-penten-1-ol (5), and (4E)-1,5-bis(4-hydroxyphenyl)-2-(hydroxymethyl)-4-penten-1-ol (7). Compounds 1-7 were detected for the first time as constituents of galanga rhizomes and exhibited antioxidative activities against the autoxidation of methyl linoleate in bulk phase.  相似文献   

Prevalence of Salmonella spp. in pigs, chickens and ducks in the Mekong Delta, Vietnam   总被引：1，自引：0，他引：1  

Tran TP  Ly TL  Nguyen TT  Akiba M  Ogasawara N  Shinoda D  Okatani TA  Hayashidani H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):1011-1014

In order to determine the prevalence of Salmonella spp. in domestic animals in 6 provinces of the Mekong Delta, Vietnam, 1,098 fecal or intestinal content samples from pigs, chickens, and ducks were examined in the period from July to October, 2000. Salmonella spp. were isolated from 78 (7.1%) of the total samples, which included 23 (5.2%) of 439 pigs, 24 (7.9%) of 302 chickens, and 31 (8.7%) of 357 ducks. From those samples, 80 Salmonella strains were isolated and 25 serovars were identified. The predominant serovars were S. Javiana, S. Derby, and S. Weltevreden. S. Javiana and S. Weltevreden were detected together in pigs, chickens, and ducks. These results indicate that the serovars of Salmonella are widely distributed in domestic animals in the Mekong Delta, Vietnam.  相似文献   

Development of EST-SSR markers of Ipomoea nil     

Tong Ly  Hiroyuki Fukuoka  Asami Otaka  Atsushi Hoshino  Shigeru Iida  Eiji Nitasaka  Nobuyoshi Watanabe  Tsutomu Kuboyama 《Breeding Science》2012,62(1):99-104

Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-containing ESTs were identified. Primer pairs were designed automatically in 326 SSRs. Of 150 SSRs examined, 75 showed polymorphisms among strains. A phenogram based on the SSR genotypes revealed the genetic relation among seven Japanese morning glories from five different regions of the world and an ivyleaf morning glory (I. hederacea Jacq.). The developed SSR markers might be applicable for genetic studies of morning glories and their relatives.  相似文献   

Effects of rice straw,biochar and mineral fertiliser on methane (CH<Subscript>4</Subscript>) and nitrous oxide (N<Subscript>2</Subscript>O) emissions from rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.) grown in a rain-fed lowland rice soil of Cambodia: a pot experiment     

Proyuth Ly  Quynh Duong Vu  Lars Stoumann Jensen  Arjun Pandey  Andreas de Neergaard 《Paddy and Water Environment》2015,13(4):465-475

We studied the effects of water regimes and nutrient amendments on CH₄ and N₂O emissions in a 2 × 3 factorial, completely randomised growth chamber experiment. Treatments included continuously flooded (CF) and alternate wetting and drying (AWD), and three organic amendments: no amendment-control, rice straw (RS) and biochar (BC). Compound fertiliser was applied to all treatments. Rice was grown in columns packed with a paddy soil from Cambodia. Results revealed faster mineralisation of organic carbon (RS and BC) when applied in water-saturated conditions lasting for 2 weeks instead of flooding. This resulted in lower total CH₄ emissions in treatments under AWD than those under the CF water regime, namely 44 % in RS treatments and 29 % in BC treatments. Nitrous oxide fluxes were generally non-detectable during the experimental period except after fertilisation events, and the total N₂O–N emissions accounted for on average 1.7 % of the total applied mineral fertiliser N. Overall, the global warming potentials (GWPs) were lower in treatments under AWD than those under the CF water regime except for the control treatment with only mineral fertiliser application. Grain yields were slightly higher in treatments under AWD than the CF water regime. Hence, the yield-scaled GWP was also lower in the treatments under the AWD water regime, namely 51 % in RS, 59 % in BC and 17 % in control treatments. Control treatments had the lowest GWP, but provided the highest yield. The yield-scaled GWP under these treatments was therefore lower than under the other treatments.  相似文献   

Evaluation of the microbiological safety and quality of Vietnamese <Emphasis Type="Italic">Pangasius hypophthalmus</Emphasis> during processing by a microbial assessment scheme in combination with a self-assessment questionnaire     

Anh?Ngoc?Tong Thi  Liesbeth?Jacxsens  Bert?Noseda  Simbarashe?Samapundo  Binh?Ly?Nguyen  Marc?Heyndrickx  Frank?DevlieghereEmail author 《Fisheries Science》2014,80(5):1117-1128

Pangasius hypophthalmus (referred to as Pangasius or tra fish in Vietnam) has become highly appreciated by consumers in the European Union, USA, Japan, etc. and is of worldwide economic importance. Therefore, the microbial quality and safety of Pangasius fish processed for export was assessed by means of a microbial assessment scheme throughout the entire production process. A total of 144 samples were collected from various processing steps and analyzed. It was determined that the microbial safety and quality of the products was not guaranteed as the contamination levels remained high throughout processing. Escherichia coli, Staphylococcus aureus and Vibrio cholerae were present on the hands of food operators, particularly those in the packaging area. Moreover, the presence of Listeria monocytogenes (1 positive out of 9 samples) and V. cholerae (4/9) on the final products was likely a result of inadequate hygiene practices in the processing environment. Also discussed in this paper are the results of a self-assessment questionnaire, which provide insight into the performance of the food safety management system currently implemented at the company. These data are of major importance in order to contribute valuable information to the local and international trade point of view in general and to the intended customers in particular.  相似文献   

交替饲喂整粒小麦或含蛋白质及矿物质浓缩的饲料提高蛋鸡饲料的利用效率     

Fisher  AD  Niemeyer  DO  Lea  JM  Lee  C  Paull  DR  Reed  MT  Ferguson  DM  洪家祥 《饲料博览》2010,(8):50-50

对16～46周龄的蛋鸡生产性能的研究表明,交替饲喂或喂混合饲料的效果是不同的。一种方法是利用同等比例的整粒小麦或营养均衡的含蛋白质及矿物质浓缩的饲料交替饲喂ISA布朗母鸡,另一种方法是饲喂混合饲料。对照组喂传统的饲料。每组16笼,每笼中5只鸡。每天光照时间16h。控制饲料的饲喂量,121g·只-·1d-1,每天饲喂2次,分别为开灯后4h和11h。在交替饲喂时,仅第1次喂食小麦,第2次喂食营养的均衡饲料。喂混合饲料时,两种定量配给的饲料混合喂2次。在下一次喂食前拿走剩余的饲料。与喂混合饲料和对照组相比,交替饲喂减少总采食量,小麦减少9g·只-·1d-1,但喂均衡饲料增多1.7g·只-·1d·-1。在3种方式中,鸡蛋的生产,蛋的大小,蛋的重量均相似。交替饲喂与喂混合饲料相比,饲料利用率的提高分别为10%和5%。与喂混合饲料和对照组相比,交替饲喂ME低,CP摄取量减少。交替饲喂时蛋鸡体重轻,其肌胃、胰腺和肝脏较重,肝脏脂质类似。喂混合饲料肝糖最高。试验结论为,交替饲喂整粒小麦和营养均衡的饲料或喂混合饲料对蛋鸡生产无负面影响。因此,两种饲喂方法是可以替代传统的喂养方式。与喂混合饲料相比,交替饲喂饲料的利用效率高,因而是可行的。  相似文献   

Evaluation of antioxidant activities and levels of biogenic monoamines of plants grown in soil amended with chitin-enriched compost harboring chitinase-producing bacteria (CECB)     

Dalin Ly  Kyoungwhan Back  In Seon Kim  Kil-Yong Kim 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):379-384

  相似文献