The effect of erector spinae plane block on perioperative analgesic consumption and complications in dogs undergoing hemilaminectomy surgery: a retrospective cohort study     

Diego A. Portela  Marta Romano  Gustavo A. Zamora  Fernando Garcia-Pereira  Luisito S. Pablo  Bonnie J. Gatson  Alana N. Johnson  Pablo E. Otero 《Veterinary anaesthesia and analgesia》2021,48(1):116-124

ObjectiveTo compare the perioperative use of analgesics and complication rates in dogs administered an erector spinae plane (ESP) block or a traditional opioid-based (OP) treatment as part of analgesic management during hemilaminectomy.Study designRetrospective cohort study.AnimalsMedical records of 114 client-owned dogs.MethodsGeneral data included demographics, duration of procedure, number of laminae fenestrated, perioperative use of steroid and non-steroidal anti-inflammatory drugs. Intra- and postoperative analgesics used in 48 hours and complications rates were compared between groups. Opioid use was expressed in morphine equivalents [ME (mg kg^?1)]. Continuous data were compared using the Mann–Whitney U test and incidence of events with a Fisher's exact tests. Multiple linear regression was used to evaluate association between perioperative ME consumption (dependent variable) with other independent variables. Data are presented as median (range). Differences were considered significant when p < 0.05.ResultsGroup ESP comprised 42 dogs and group OP 72 dogs. No differences were observed in the general data. Intraoperative ME was 0.65 (0.20–3.74) and 0.79 (0.19–5.60) mg kg^?1 in groups ESP and OP, respectively (p = 0.03). Intraoperative infusion of lidocaine was administered intravenously (IV) to 23.8% and 68% of groups ESP and OP, respectively (p < 0.0001). Intraoperative infusion of ketamine was administered IV to 21% and 40% of groups ESP and OP, respectively (p = 0.04). Regression analysis revealed the ESP block as the only independent variable affecting the perioperative ME consumption. Pharmacological intervention to treat cardiovascular complications was administered to 21.4% and 47.2% of dogs in groups ESP and OP, respectively (p = 0.008). There were no differences in postoperative complication rates.Conclusions and clinical relevanceESP block was associated with reduced perioperative opioid consumption, intraoperative adjuvant analgesic use and incidence of pharmacological interventions to treat cardiovascular complications in dogs undergoing hemilaminectomy.  相似文献   

Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection.   总被引：4，自引：0，他引：4  

Martín J Zumárraga  Virginia Meikle  Amelia Bernardelli  Alejandro Abdala  Hector Tarabla  María I Romano  Angel Cataldi 《Journal of veterinary diagnostic investigation》2005,17(3):232-238

The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis-positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.  相似文献   

Perosomus elumbis in a Holstein calf in Brazil     

Castro MB  Szabó MP  Hokamura HK  Romano MA 《The Veterinary record》2003,152(24):753

  相似文献   

Follicular Dynamics in Heifers during Pre-pubertal and Pubertal Period Kept under Two Levels of Dietary Energy Intake   总被引：1，自引：0，他引：1  

MA Romano  VH Barnabe  JP Kastelic  CA de Oliveira  RM Romano 《Reproduction in domestic animals》2007,42(6):616-622

The objective of this study was to characterize follicular dynamics in pre-pubertal, pubertal and post-pubertal periods, as well as the effect of high-energy intake on follicular development and age at puberty in heifers. Thirty-one Nelore (Bos indicus) heifers, 6 months old, were randomly assigned to receive two different diets: one of low (GI) and other of high dietary energy intake (GII). Animals were evaluated in relation to body weight gain by being weighed every 21 days. Heifers were evaluated every other day by real-time linear ultrasonography to characterize ovarian structures development from weaning to post-pubertal period. Blood samples were collected to determine plasmatic concentrations of progesterone by RIA method. The ovulation was determined when progesterone concentrations were >1 ng/mL in three consecutive samples, and by ultrasound images of corpus luteum; and oestrous behaviour in some animals. Age at puberty differed among heifers of GII (17.00 +/- 0.46 months) compared with heifers of GI (19.87 +/- 0.47 months; p < or = 0.05). Maximum size of the dominant follicles at pre-pubertal period was greater in GII heifers than in GI (10.52 +/- 0.33 and 9.76 +/- 0.15 mm, respectively; p < or = 0.05). As heifers approached first ovulation time, size of dominant follicle increased (11.75 +/- 0.37 mm for GI and 12.52 +/- 0.91 mm for GII; p < or = 0.05). Body weight at puberty was not different in both groups (302.33 +/- 27.31 kg for GI and 326.19 +/- 27.78 kg for GII heifers; p > 0.05). We conclude that animals receiving high dietary energy intake attained the puberty earlier and the development of follicles were different than in low dietary energy intake.  相似文献   

A new hemolytic assay for bovine serum complement and its application during experimental bovine anaplasmosis     

A M Soler-Rodríguez  E Romano  Y Aranguren  A Soyano 《Veterinary immunology and immunopathology》1990,24(4):347-360

A new hemolytic assay for bovine complement is presented. Using this assay we found a significant reduction in bovine serum complement activity during the acute phase of anaplasmosis, and an increase in the sensitivity of the red blood cells (RBC) to bovine complement lysis in vitro. The new hemolytic test is performed with bovine RBC, rabbit anti-bovine RBC serum and bovine serum complement. An isotonic sucrose Tris-buffered saline solution of ionic strength 0.094 and pH 7.2 was found to be adequate for this test. The titres obtained with this new assay, which uses autologous RBC, are comparable with those obtained using the guinea pig RBC assay. The finding of a reduction in bovine serum complement during anaplasmosis may be suggestive of a mechanism responsible for the pathology of this disease.  相似文献   

Phenological investigations of different winter-deciduous species growing under Mediterranean conditions     

Fabio Orlandi  Tommaso Bonofiglio  Luigia Ruga  Carlo Sgromo  Bruno Romano  Marco Fornaciari 《Annals of Forest Science》2007,64(5):557-568

Phenological stages are the result of biorhythms and environmental factors, these last are probably the same ones that caused, during evolution, adjustments of the species to different climate. The present study was carried out in a Phenological Garden located in central Italy (Perugia, Umbria Region) which contains indicator species, common to all International Phenological Gardens. The aim of this study was to determine and analyse the average trends of development of eight plant species and their phenological adjustment to the Mediterranean environment, over a nine-year period (1997–2005). The results of the statistical analyses show a strong relationship between the temperature trends and vegetative seasonal evolutions interpreted by phenological data for all the species considered. Moreover, it was demonstrated that the plants studied may approach or close completely the timing gaps eventually created during the first phenological phases, adjusting thus the beginning of subsequent phenophases.  相似文献   

Histopathological effects of cadmium on the gills of the freshwater fish, Macropsobrycon uruguayanae Eigenmann (Pisces, Atherinidae)     

A S Randi  J M Monserrat  E M Rodriguez  L A Romano 《Journal of fish diseases》1996,19(4):311-322

Evaluations of histopathological lesions in gill tissue were carried out in the freshwater fish Macropsobrycon uruguayanae following 30 and 60 days of exposure to 1.5 mg 1^-1 of cadmium. The study was conducted on both fed and starved animals in order to determine the influence of feeding condition on cadmium toxicity. The main lesions observed and quantified were: (1) hyperplasia of primary lamellar epithelium; (2) hyperplasia of secondary lamellar epithelium; (3) separation of respiratory epithelium; (4) shortening of secondary lamellae; (5) epithelial necrosis; (6) fusion of adjacent secondary lamellae; (7) hypertrophy of respiratory epithelium; (8) lamellar telangiectasis; (9) hyperplasia of chloride cells; (10) mucinous metaplasia; and (11) inflammatory infiltration. Lesions 6, 8, 9 and 11 were only induced by exposure to cadmium, while lesion 4 could be produced only by starvation. Starved fish also showed a reduction in total body weight and length. Lesion 2 was shown to be non-specific, and produced by either cadmium, starvation or even exposure time. The possible mode of action of the experimental factors are discussed in relation to the observed pathologies.  相似文献   

Infrared Thermal Imaging as a Rapid Tool for Identifying Water‐Stress Tolerant Maize Genotypes of Different Phenology     

S. Zia  G. Romano  W. Spreer  C. Sanchez  J. Cairns  J. L. Araus  J. Müller 《Journal of Agronomy and Crop Science》2013,199(2):75-84

The main task of this research was to evaluate canopy temperature and Crop Water Stress Index (CWSI) by assessing genotype variability of maize performance for different water regimes. To that end, three hundred tropical and subtropical maize hybrids with different phenology in terms of date of anthesis were evaluated. The influence of phenology on the change in canopy temperatures and CWSI was not equal during the three dates of measurement. At the end of vegetative growth (82 days after sowing, DAS) and at the blister stage (DAS 97), a high significant difference in temperatures and CWSI (P < 0.001) were obtained between the early‐ and late‐maturity genotypes. During anthesis (DAS 89), phenology had a significant effect (P < 0.01) only for the well‐watered genotypes, while under water‐stress conditions, no differences were found between early and late genotypes in terms of canopy temperature and CWSI. High significant differences (P < 0.001) in stomatal conductance (g_s) between early and late genotypes for different treatments were observed. A relationship (R² = 0.62) between g_s and canopy temperature was obtained. Under a water‐stress canopy, temperature was measured at anthesis, which was negatively correlated with grain yield of the early (r = ?0.55)‐ and late (r = ?0.46)‐maturity genotypes in the water‐stressed condition.  相似文献   

Nitrogen metabolism components as a tool to discriminate between organic and conventional citrus fruits     

Rapisarda P  Calabretta ML  Romano G  Intrigliolo F 《Journal of agricultural and food chemistry》2005,53(7):2664-2669

The aim of this work was to develop a method for authenticity control of organically grown orange fruits. Due to the different kinds of nitrogen fertilization of the soil in organically and conventionally managed farms, the study tried to verify the possibility to differentiate Navelina and Tarocco orange fruits obtained by these production systems through the detection of markers linked to nitrogen metabolism. In addition to the classic quality parameters, total nitrogen (N) and synephrine contents in juice and (15)N/(14)N isotope ratio (expressed as delta(15)N per thousand) in proteins of pulp and amino acids of juice were determined. The results obtained indicated that total N and synephrine contents were significantly higher in conventional fruits, whereas the delta(15)N per thousand values were higher in the organic ones. The new markers identified in this research by linear discriminant analysis of the data may constitute a useful tool to differentiate organic citrus fruits or juices from conventional ones.  相似文献   

High levels of genetic differentiation between Wolbachia-infected and non-infected populations of Folsomia candida (Collembola, Isotomidae)     

Francesco Frati  Ilaria Negri  Pietro P. Fanciulli  Marco Pellecchia  Vincenzo De Paola  Valerio Scali  Romano Dallai 《Pedobiologia》2004,48(5-6):461-468

The Gram-negative -Proteobacterium Wolbachia pipientis has been described as an obligate endosymbiont in many arthropod species, where it induces a variety of reproductive alterations, including parthenogenesis. Recently, this microorganism has also been detected in the parthenogenetic collembolan Folsomia candida. Here, we confirm the occurrence of the endosymbiont also in two Italian parthenogenetic populations of F. candida using ultrastructural (electron microscopy) and molecular (PCR screening on two bacterial genes) evidence. The strain isolated in the Italian populations has almost-identical gene sequences compared to that previously isolated in other populations of F. candida. In addition, we discovered a population of Folsomia cf. candida, which showed the presence of both males and females. This population is not infected by Wolbachia. A screening of two mitochondrial genes (COI and COII) showed that the bisexual population has high levels of genetic divergence in comparison with the parthenogenetic ones, even suggesting the possibility that it belongs to a different species. Furthermore, the remarkably high levels of genetic divergence between the two parthenogenetic populations suggests a possible influence of Wolbachia on inducing such differentiation, and, in the long term, speciation.  相似文献