Apoptosis in the Antral Follicles of Swamp Buffalo and Cattle Ovary: TUNEL and Caspase-3 Histochemistry     

JB Feranil  N Isobe  T Nakao 《Reproduction in domestic animals》2005,40(2):111-116

The present study was carried out to investigate the pattern of apoptosis in the healthy antral and atretic follicles of Philippine swamp buffaloes (BU) in comparison with Holstein-Friesian (HF) cows. Paraffin sections of healthy follicles and various stages of atretic follicles were stained using the terminal deoxynucleotidyl transferase (TdT)-mediated biotinylated deoxyuridine triphosphates (dUTP) nick end-labelling (TUNEL) method to detect DNA fragmentation and cleaved caspase-3 antibody to detect cells committed to undergo apoptosis. Five equidistant areas of a follicle were counted for the presence of TUNEL- and caspase-3-positive cells. Healthy follicles of BU and HF contained no TUNEL-positive cells in the granulosa and theca layer but showed some caspase-3 positivity. The granulosa layer of advanced atretic follicles showed a significantly higher frequency of caspase-3 positivity than the healthy and early atretic follicles in both breeds. The frequency of caspase-3-positive cells of BU was significantly higher than HF in the granulosa layer of healthy, early atretic and advanced atretic follicles. In the theca interna layer, BU and HF showed a significantly lower and higher frequency of TUNEL-positive cells in the late atretic follicles compared with advanced atretic follicle, respectively. However, the frequency of caspase-3-positive cells of both BU and HF in the late atretic follicles was significantly higher than the advanced atretic follicles in the theca interna layer. These results indicate that caspase-3 and DNA fragmentation is involved in the buffalo ovarian apoptotic process.  相似文献   

Cell Proliferation in the Atretic Follicles of Buffalo and Cattle Ovary     

JB Feranil  N Isobe  T Nakao 《Reproduction in domestic animals》2004,39(6):405-409

The present study was carried out to describe the proliferative activity of granulosa and theca cells in healthy antral and atretic follicles of Philippine buffaloes (BU) and Holstein-Friesian (HF) cows. Paraffin sections of ovary were immunostained with mouse monoclonal antibody to proliferating cell nuclear antigen (PCNA). Then the follicles were classified into healthy and various stages of atretic follicles. The granulosa layer of healthy follicles had a significantly higher frequency of PCNA-positive cells than the early and advanced atretic follicles in both breeds. In the theca interna, significantly reduced populations of the PCNA-positive cells were found in both breeds as atresia progressed. Moreover, HF had significantly higher PCNA-positive cells in the theca interna of healthy, early atretic and advanced atretic follicles than BU. A reduction of PCNA-positive cells during atresia was also noted in the theca externa in both animals although differences were not significant. The results of the present work suggest that the proliferative activity of granulosa and theca cells decreases in association with follicular atresia in the BU similar to HF. Furthermore, a significantly deficient cell proliferative activity of theca interna was found in BU compared with HF.  相似文献   

Ergovaline does not alter the severity of ryegrass staggers induced by lolitrem B     

SC Finch  JB Vlaming  BL Sutherland  C van Koten  WJ Mace  LR Fletcher 《New Zealand veterinary journal》2018,66(2):93-97

AIM: To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.

METHODS: Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with either AR98 endophyte (containing lolitrem B), standard endophyte (containing lolitrem B and ergovaline) or no endophyte, for up to 42 days from 2 February 2010. Ten lambs were grazed on three replicate plots per cultivar. Herbage samples were collected for alkaloid analysis and lambs were scored for ryegrass staggers (scores from 0–5) weekly during the study. Any animal which was scored ≥4 was removed from the study.

RESULTS: Concentrations of lolitrem B did not differ between AR98 and standard endophyte-infected pastures during the study period (p=0.26), and ergovaline was present only in standard endophyte pastures. Ryegrass staggers was observed in sheep grazing both the AR98 and standard endophyte plots, with median scores increasing in the third week of the study. Prior to the end of the 42-day grazing period, 22 and 17 animals were removed from the standard endophyte and AR98 plots, respectively, because their staggers scores were ≥4. The cumulative probability of lambs having scores ≥4 did not differ between animals grazing the two pasture types (p=0.41).

CONCLUSIONS AND CLINICAL RELEVANCE: There was no evidence for ergovaline increasing the severity of ryegrass staggers induced by lolitrem B. In situations where the severity of ryegrass staggers appears to be greater than that predicted on the basis of concentrations of lolitrem B, the presence of other tremorgenic alkaloids should be investigated.  相似文献   

Platynosomum concinnum infection in cats in Nigeria     

B O Ikede  G J Losos  T T Isoun 《The Veterinary record》1971,89(24):635-638

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Pathological changes in cattle infected with Trypanosoma brucei   总被引：1，自引：0，他引：1  

B O Ikede  G J Losos 《Veterinary pathology》1972,9(4):272-277

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Cryopreservation of Piau‐Breed Wild Boar Sperm: Assessment of Cooling Curves and Centrifugation Regimes          下载免费PDF全文

HH Shiomi  RO Pinho  DMA Lima  JB Siqueira  MCR Santos  EV Costa  PS Lopes  SEF Guimarães  JD Guimarães 《Reproduction in domestic animals》2015,50(4):545-553

This study aimed to assess the effects of different cooling curves and centrifugation regimes used in cryopreservation protocols on the post‐thaw viability of Piau‐breed wild boar (Sus scrofa) sperm using in vitro assessment tests. Two centrifugations (800  g for 10 min and 2400  g for 3 min) and two cooling curves (conventional cooling using nitrogen vapour – freezing 1 and automated cooling using a programmed freezing machine – freezing 2) were tested. Therefore, the treatments were divided into M3 – centrifugation at 2400  g for 3 min and freezing 2; M10 – centrifugation at 800  g for 10 min and freezing 2; R3 – centrifugation at 2400  g for 3 min and freezing 1; and R10 – centrifugation at 800  g for 10 min and freezing 1. No significant differences (p > 0.05) between treatments occurred post‐thawing regarding the total sperm motility means recorded. The mean values of the different treatments were not different from each other regarding the supravital staining (SV), hypo‐osmotic test (HO), sperm–egg binding assay or sperm morphology. This study showed that both the cooling curve and the centrifugation regime affected the quality of post‐thaw sperm, and centrifugation for shorter times and cooling curves using automated cooling are the most suitable for minimizing sperm injury.  相似文献   

Spatial patterns in the distribution of tropical tree species   总被引：1，自引：0，他引：1  

Condit R  Ashton PS  Baker P  Bunyavejchewin S  Gunatilleke S  Gunatilleke N  Hubbell SP  Foster RB  Itoh A  LaFrankie JV  Lee HS  Losos E  Manokaran N  Sukumar R  Yamakura T 《Science (New York, N.Y.)》2000,288(5470):1414-1418

Fully mapped tree census plots of large area, 25 to 52 hectares, have now been completed at six different sites in tropical forests, including dry deciduous to wet evergreen forest on two continents. One of the main goals of these plots has been to evaluate spatial patterns in tropical tree populations. Here the degree of aggregation in the distribution of 1768 tree species is examined based on the average density of conspecific trees in circular neighborhoods around each tree. When all individuals larger than 1 centimeter in stem diameter were included, nearly every species was more aggregated than a random distribution. Considering only larger trees (>/= 10 centimeters in diameter), the pattern persisted, with most species being more aggregated than random. Rare species were more aggregated than common species. All six forests were very similar in all the particulars of these results.  相似文献   

A comparative study of the diseases in cattle caused by Theileria Lawrencei and Theileria Parva. 1. Clinical signs and parasitological observations     

W.G.Z. Jura  G.J. Losos 《Veterinary parasitology》1980,7(4):275-286

A comparison was made between the clinical signs and blood and lymph node parasite levels in cattle reacting to the inoculation of tick-derived stabilates of Theileria lawrencei (TL), and T. parva at high dose (TPH) and low dose (TPL) levels. The TL and TPH infections produced acute diseases with statistically similar tines to death of 13.50 and 14.25 days, respectively, while TPL caused a more protacted but equally fatal disease of 20 days duration. In the TPL group, the local drainage lymph node (LDLN) became enlarged earlier and macroschizonts appeared later than in the TPH group. The characteristics of T. lawrencei infection which differentiated it from T. parva infection were: nervous signs; complete anorexia; low macroschizont index (MSI) levels of up to 5%; and lack of piroplasms. In T. parva groups, the high MSI levels of up to 60%, presence of piroplasms and dysentery were characteristic. In addition, the enlargement of lymph nodes in the TL group persisted to time of death, while in both T. parva infections the initial enlargement was followed by a decrease in size.The 100-fold dose difference between TPH and TPL influenced the times of onset of the majority of clinical signs, most of which occurred later in the TPL group. The MSI levels were 60% in the TPH group and 40% in the TPL group. The piroplasm parasitemia levels reached 5% in the TPH group, and 16% in the TPL group.Theileria lawrencei and T. parva caused diseases with significant differences in clinical manifestations and parasite levels in erythrocytes and lymphocytes. The size of the infecting dose influenced the time of onset of clinical signs as well as the duration of the disease.  相似文献   

Molecular pathotyping of two avian influenza viruses isolated during the Victoria 1976 outbreak   总被引：3，自引：0，他引：3  

JB BASHIRUDDIN  AR GOULD  HA WESTBURY 《Australian veterinary journal》1992,69(6):140-142

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Complication after the use of a combination of lignocaine and xylazine for epidural anaesthesia in a mare     

JB CHOPIN  JD WRIGHT 《Australian veterinary journal》1995,72(9):354-355

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