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Summary The gene df (deformed flower), which is expressed as short anther in sensitive cytoplasm, has been incorporated into the trisomic series of S. chacoense
Bitt. The cytoplasmic sensitivity and genotypes for the gene df were tested in the original trisomics and their parents. Two diploid parents were found to be homozygous dominant for Df, while one was homozygous recessive. All the parents and trisomics tested had resistant cytoplasm. Six trisomics were heterozygous for df, segregating with a 1:1 ratio when they were crossed to the homozygous testers ([df
s]dfdf). When the F1 trisomics (trisomics x [Df
r]dfdf) were crossed to the homozygous testers, nine of the 13 trisomics segregated with a 1:1 ratio, three with a 0:1 ratio (all deformed) and one trisomic (V1682.3) with a 2:1 ratio. All the parents of the trisomic V1682.3 were homozygous dominant for Df. This clone is believed to be trisomic for the gene df.Cooperative investigation of the Agricultural Research Service, U.S. Department of Agriculture and the Wisconsin Experiment Station, Madison. 相似文献
2.
D. W. S. Mok Heiyoung K. Lee S. J. Peloquin 《American Journal of Potato Research》1974,51(10):337-341
A modified Giesma staining technique was used to identify the somatic chromosomes of diploid potatoes. Distinct banding patterns were observed on all 12 chromosomes. Individual chromosomes were identified according to the number and distribution of bands. No significant difference in banding patterns was observed between the chromosomes of Phureja-haploid Tuberosum hybrids and those ofS. chacocnse. The extra chromosome of a trisomic of 5.chacoense was identified as an isochromosome for the long arm of chromosome G. 相似文献
3.
Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato andSolanum commersonii 总被引：1，自引：0，他引：1
Heiyoung Kim-Lee J. S. Moon Y. J. Hong M. S. Kim H. M. Cho 《American Journal of Potato Research》2005,82(2):129-137
Bacterial wilt of potatoes, which used to be a widespread disease in tropics and subtropics, has become a threat to potato production in temperate region. The diploid species Solanum commersonii has several desirable characteristics including cold tolerance and resistance to several diseases. Selected somatic hybrids between S. tuberosum dihaploid and bacterial-wilt-resistant S. commersonii clones were cross pollinated with S. tuberosum cultivars for further selection of bacterial wilt resistance. The chromosome numbers of the fusion parents were confirmed as 24, and the three fusion hybrids crossed were all tetraploids. The chromosome number of 11 backcross 1 progenies (BC1) was 48 and that of the other six was close to the tetraploid number. Backcross 2 progenies (BC2) were obtained from only three of the 44 BC1 clones crossed. The S. commersonii parent clone, LZ3.2, was the most resistant to bacterial wilt among wild species clones tested. The first sexual progenies segregated for resistance, with one clone highly susceptible and four clones highly resistant. Three highly resistant BC1 clones, CT02-4, CT08-4, and CT10b-4, were backcrossed to cultivars. Two cross combinations produced mostly susceptible BC2 progenies; however seven clones were resistant or highly resistant for both race 1 and race 3. The highly resistant three clones, CT204-3, CT206-9 and CT206-10, were selected for the further testing as cultivars or breeding materials. 相似文献
4.
Yu-Kuang H. Chen Jiwan P. Palta John B. Bamberg Heiyoung Kim Geraldine T. Haberlach John P. Helgeson 《Euphytica》1999,107(1):1-8
The expression of freezing tolerance was characterized in interspecific somatic hybrids between S. tuberosum (tbr) and two
cold-hardy wild species, S. brevidens (brd) and S. commersonii (cmm). The nonacclimated freezing tolerance (NA) and acclimation
capacity (ACC, increase in freezing tolerance in response to low nonfreezing temperature), two main genetic components of
freezing tolerance, were evaluated separately. In contrast to cmm, which exhibited excellent NA and ACC, the freezing tolerance
of brd was mainly due to ACC. However, the ACC of brd was only moderately expressed in the somatic hybrids. The NA of cmm
was also suppressed in combination with tbr genomes. However, with acclimation, some of the tbr (+) cmm somatic hybrids achieved
freezing tolerance comparable to pure hardy species such as brd used in this study. Analysis of chloroplast DNA type by RFLP
markers revealed no significant difference in ACC between somatic hybrids carrying chloroplasts from either tbr or cmm. The
reasons for the reduced expression of freezing tolerance from either the brd or cmm parent and the utilization of these somatic
hybrids in breeding programs are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
5.
Development of an ELISA using a recombinant 41 kDa partial protein (P45N') for the detection of Riemerella anatipestifer infections in ducks 总被引：5，自引：0，他引：5
Riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. An indirect enzyme-linked immunosorbent assay (ELISA) has been developed to facilitate early detection of R. anatipestifer infection in ducks. The antigen used was a recombinant 41 kDa N-terminal fragment (rP45N') of a newly characterized R. anatipestifer potential surface protein, P45, which was expressed in Escherichia coli as an N-terminal GST fusion protein. The rP45N'-based ELISA successfully detected P45 antibodies in the sera of 20 ducks immunized with bacterin preparations of R. anatipestifer serotypes 1, 10 15, 19 and the ATCC11845 strain. Antibodies to P45 were also detected in the sera of 25% (75/296) of White Pekin ducks which were imported into Singapore from three different farms. Successful discrimination was obtained between sera from infected ducks and that of specific-pathogen free ducks (p<0.01). The rP45N'-GST antigen did not cross-react with antibodies in sera from guinea pigs which were infected with other gram-negative and gram-positive bacterial pathogens, including Aeromonas hydrophila, Citrobacter freundii, E. coli, Klebsiella pneumoniae, Pastuerella multocida, Proteus mirabilis, Salmonella spp., Serratia maccescens, Shigella sonnei and Yersinia enterocolitica. In addition, the DNA sequence encoding P45 was detected in R. anatipestifer serotypes 1, 2, 3, 4, 5, 6, 7, 8, 9, 11, 14, 15, 16, 17, 18, 19 and the ATCC11845 strain, suggesting that P45 is probably also universally expressed in these R. anatipestifer serotypes. Thus, the ELISA described is applicable to the detection of R. anatipestifer infection in ducks. 相似文献
6.
Vaccination of ducks with recombinant outer membrane protein (OmpA) and a 41 kDa partial protein (P45N') of Riemerella anatipestifer. 总被引：9，自引：0，他引：9
Bin Huang Sumathi Subramaniam Joachim Frey Hilda Loh Hai-Meng Tan Charlene J Fernandez Jimmy Kwang Kim-Lee Chua 《Veterinary microbiology》2002,84(3):219-230
The generation of protective immunity against Riemerella anatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (GST) fusion's proteins of OmpA, a 42kDa major outer membrane protein, and P45N', a 41kDa N-terminal fragment of a newly identified 45kDa potential surface protein from R. anatipestifer. The DNA encoding OmpA and P45N' were isolated from R. anatipestifer serotype 15 (field strain 110/89) and serotype 19 (reference strain 30/90), respectively. Immunoblotting and ELISA results showed that the purified recombinant proteins induced the production of antibodies in immunized ducks. However, neither was protective against subsequent challenge with the virulent serotype 15 strain, 34/90. All the five ducks immunized with formalinized R. anatipestifer strain 34/90 survived the challenge with the homologous strain whereas six out of seven ducks in the non-immunized control group died within a week following the challenge. 相似文献
7.
Summary The triploids recovered from 4x×2x crosses in three Solanum species were very vigorous and although few seeds/fruit were obtained when the triploids were crossed to diploids, the extensive crossing programme produced sufficient seed of four species cph, chc, pnt and tar. The average seed set for the 3x-2x crosses was an extremely low 3.5 seeds/fruit.Approximately 90% of the progeny of the 3x–2x crosses were aneuploids with chromosome numbers of 2n=25–29. The frequency of the aneuploids in the three species that were studied was chc 93%, pnt 94% and tar 92%. The aneuploids of chc and tar were extremely vigorous and fertile and they were used as females in crosses to the marker stocks. The aneuploids of pnt were vigorous, but the crossability barrier of pnt prevented their use in crosses to the marker stocks. A number of the aneuploids produced seed upon being selfed, but the ability to produce self seed may be related to the pseudo-compatibility of the parental clones. In only 7 aneuploids was there an indication that the self fertility was due to overcoming the self-incompatibility barrier as a result of competition-interaction of the aneuploid gamete.Plants in the families from the triploid-diploid crosses had a tremendous amount of variation in morphological characteristics (leaf shape, size and color; berry shape, color and degree or verrucose spotting: and plant habit and vigor). A large portion of variation exhibited in these families was due to the normal genetic segregation of the heterozygous parents. It was impossible to distinguish the aneuploids from their diploid sibs especially those having only one or two additional chromosomes because they were as vigorous and fertile as the diploids.There were some preliminary indications of the existence of distinct morphological characteristics among the aneuploids (separate petals, long berries, and extreme verrucose berries). However, there was no indication that these traits were due to the dosage effect of the extra chromosome. If it should be determined that this was true, it would be extremely useful in associating genes with chromosomes and determining the phenotypic effects due to the presence of an additional chromosome.Cooperative investigations of the College of Agriculture and Life Sciences, University of Winconsin and the Agricultural Research Service, U.S. Department of Agriculture, Madison, Wisconsin. Supported in part by grants from the Research Committee of the Graduate School, University of Wisconsin.First and third author respectively, formally Graduate Research Assistant Department of Horticulture, University of Wisconsin, Madison, and Geneticist Agriculture Research Service, U.S. Department of Agriculture, University of Wisconsin, Madison. Present address: Department of Genetics. University of Wisconsin, Madison, Wisconsin 53706 and Head Breeding and Genetics Department, International Potato Center, Lima, Peru. 相似文献
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