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Khattiya Akarasanon Praneet Damrongphol & Wandee Poolsanguan 《Aquaculture Research》2004,35(15):1415-1420
Long‐term cryopreservation of the giant freshwater prawn, Macrobrachium rosenbergii, spermatophores using glycerol (Gly) and ethylene glycol (EG) as cryoprotective agents (CPAs) was studied. The tolerance of sperm to cryopreservation was evaluated on the basis of sperm survival and fertilizing ability. The survival of the sperm was determined by trypan blue staining, while the fertilizing ability was assessed from artificial insemination of the cryopreserved spermatophores. The rates of embryo survival on day 5 after spawning and of spermatophores capable of producing embryos survived to hatching were determined. Storage of spermatophores at ?20°C without CPA for a short period of up of 1–5 days decreased the sperm survival significantly and did not preserve fertilizing ability. Preservation at ?20°C in the presence of 10% or 20% Gly or of 10% or 20% EG offered a simple and efficient short‐term storage up to 10 days. For a long‐term storage, cryopreservation in the presence of 20% EG at ?196°C was more efficient than at ?20°C. High sperm survival rates and high fertilizing ability were recorded from those cryopreserved at ?196°C for up to 150 days. High sperm survival rates with moderate levels of fertilizing ability were obtained from those cryopreserved at ?20°C for not more than 30 days. The results indicate that preservation at ?196°C with 20% EG is a suitable procedure for long‐term storage of the giant freshwater prawn spermatophores. 相似文献
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Praneet Damrongphol Pleanphit Jaroensastraraks Boonserm Poolsanguan 《Fisheries Science》2001,67(1):64-70
SUMMARY: The effectiveness of in vitro embryo culture of the giant freshwater prawn Macrobrachium rosenbergii depended on the age of the embryos at the onset of culture and on the concentrations of various compositions in the medium. Embryos that started being cultured on day 0.5 after oviposition were more sensitive to variations in the medium compositions than those that started being cultured on day 10.5 after oviposition. An optimal NaCl level was essential for embryonic development, survival, hatching and survival of the newly hatched larvae. Variations of NaCl or KCl levels dramatically altered embryonic development, and variation of the MgCl2 + MgSO4 level significantly lowered survival of the embryos that started being cultured at the early stage of development. In contrast, no significant change in embryonic development was observed upon variation of the CaCl2 level. Hatching of the embryos required the presence of NaCl and CaCl2 but not KCl or MgCl2 + MgSO4 . The ionic requirements of the newly hatched larvae differed from that of the developing embryos. Variations of NaCl, KCl or CaCl2 but not MgCl2 + MgSO4 levels significantly influenced the survival of the newly hatched larvae. 相似文献
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