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1.
Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow 下载免费PDF全文
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A series of 2-alkyl-7, 8-dihydro-3-hydroxynaphtho[1,2-c]chromen-6-ones was synthesised by the condensation of ethyl 3, 4-dihydro-1-oxonaphthalene-2-carboxylate with substituted phenols in the presence of POCl3. The compounds were characterised and tested for their toxicity towards the mycelial growth of seven phytopathogenic fungi in culture. Drechslera oryzae, Rhizoctonia solani and Colletotrichum falcatum exhibited maximum sensitivity to these compounds whereas Macro-phomina phaseolina, Fusarium solani, Alternaria alternata and Pythium aphanidermatum were less sensitive. 2-Ethyl-7, 8-dihydro-3-hydroxy-naphtho[1,2-c]chromen-6-ones possessed greatest toxicity with EC50 values ranging from 0.2 to 2.5 μg ml?1 against all fungi except A. alternata and P. aphanidermatum. 相似文献
3.
Kataria RS Tiwari AK Nanthakumar T Goswami PP 《Veterinary research communications》2001,25(5):429-436
A single-tube, non-interrupted, one-step RT-PCR has been standardized to amplify the hypervariable region of the VP2 gene sequence of infectious bursal disease virus (IBDV). The technique standardized on purified viral RNA was successfully applied to the detection of the virus directly in clinical samples. The amplified products were confirmed to be IBDV specific by their size in ethidium bromide-stained agarose gel, nested PCR and restriction enzyme digestion. Digestion of the amplicons with StyI restriction enzyme also differentiated classical virus from six very virulent field isolates. The sensitivity of the one-step RT-PCR was found to be 0.2 pg of viral RNA. 相似文献
4.
An isolate of Newcastle disease virus obtained from a guinea fowl was characterized as a viscerotropic velogenic strain based upon pathogenicity index studies. Following inoculation of the viral isolate oronasally into 3-week-old chickens, clinical signs appeared after an incubation period of 4–5 days and included dullness, depression, dyspnoea, diarrhoea and leg paralysis. The virus caused a mortality of 56% with haemorrhages at the tip of the glands of the proventriculus and caecal tonsil. Histopathological changes were prominent in the lymphoid organs, being characterized by depletion, degeneration and necrosis of the lymphoid tissues. The brain was the first organ affected, with changes being noticed 3 days after infection. Isolation of virus from various organs was more frequent from 5 to 10 days after infection, but the virus persisted in some of the organs until 21 days after infection. In spite of the high mortality, a good immune response was elicited by the isolate, as was evident from the antibody titre. 相似文献
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- 1. Mahseer fishes of the genus Tor are ‘iconic species’ in Indian rivers, representing ‘flagship’ species for conservation purposes but with additional exploitable, cultural and economic values. Conservation action is required to address their vulnerability to numerous pressures on multiple habitats, and connections between them, across the extended aquatic ecosystems supporting different life stages.
- 2. Recreational angling for mahseer, along with associated wildlife and cultural tourism, is a growth industry dependent upon healthy river ecosystems.
- 3. A cyclic, ecosystem‐based model in which local people can benefit economically from sustainable mahseer exploitation through catch‐and‐release fisheries can form a basis for conservation of both mahseer and the wider river ecosystem.
- 4. Experience on the Western Ramganga River in the vicinity of the town of Bikhyasen, Uttarakhand State (in the Himalayas in northern India), demonstrates the importance of involving local people in the benefits associated with angling, wildlife and cultural tourism. This creates local incentives for river protection, and may be the most effective means for preventing destructive over‐exploitation in poor rural communities.
- 5. Recognition of this value and its recirculation to local people can form the basis for a ‘paying for ecosystems services’ (PES) market. This will benefit river ecosystems, local people, tour operators and visiting anglers, provided that local people benefit to a greater extent than through the killing of fish for non‐renewable consumption or sale.
- 6. This is the basis for a potentially transferable model which may support regional development through involving local people in markets for iconic species. Copyright © 2010 John Wiley & Sons, Ltd.
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The inhibition of mycelial growth of Rhizoctonia solani in vitro was strongest with pencycuron, followed by tolclofos-methyl, carboxin and thiabendazole. Against cowpea seedling rot in soil infested with R. solani, tolclofos-methyl was most effective, followed by pencycuron, thiabendazole and carboxin. Of nine insecticides tested, only parathion-methyl and quinalphos suppressed mycelial growth of R. solani in vitro, although their activity was much lower than that of the four tested fungicides. The inhibition of mycelial growth by fungicide-insecticide mixtures was antagonistic in only two out of 36 combinations; it was additive in most cases or synergistic, e.g. for most mixtures of pencycuron and insecticides. Quinalphos, applied to the soil, was the only insecticide which reduced cowpea seedling rot in soil infested with R. solani. Soil application of parathion-methyl, phorate, aldicarb or carbofuran and seed treatment with phosphamidon, monocrotophos, endosulfan or dimethoate had little or no effect on seedling rot. Carboxin gave better disease control when applied to the seed already coated with phosphamidon, monocrotophos, endosulfan or dimethoate and when carboxin-treated seeds were sown in soil treated with quinalphos, parathion-methyl, aldicarb or carbofuran. Efficacy of thiabendazole seed treatment was slightly higher in the presence of insecticides, particularly dimethoate. Pencycuron and tolclofos-methyl as seed treatment gave nearly 100% disease control both in the presence and absence of insecticides. The synergistic interactions detected between fungicides and insecticides represent interesting opportunities for the control of R. solani. 相似文献
9.
The herbicides fluchloralin and alachlor applied to soil altered the effectiveness of fungicide treatments to seed and soil for controlling cowpea damping-off. These herbicides also modified the in-vitro toxicity of the fungicides to the mycelial growth of Pythium butleri and Rhizoctonia solani in a nutrient medium. Both herbicides reduced the toxicity of 2-methoxyethylmercury chloride (MEMC) and propamocarb to the growth of P. butleri, and of carbendazim to the growth of R. solani, but enhanced the toxicity of captafol and quintozene to P. butleri and R. solani, respectively. In pot tests, quintozene gave better control of R. solani damping-off in soil treated with fluchloralin or alachlor than in untreated soil, whereas disease control by carbendazim was decreased in similarly treated soils. Both herbicides attenuated the effectiveness of MEMC and captafol to control the damping-off caused by P. butleri; the efficacy of propamocarb was increased by alachlor but was decreased by fluchloralin. The implications of herbicide-fungicide interactions are discussed in the context of fungicidal control of root diseases in herbicide-treated soil. 相似文献
10.
Sharma AK Kumar R Kumar S Nagar G Singh NK Rawat SS Dhakad ML Rawat AK Ray DD Ghosh S 《Veterinary parasitology》2012,188(3-4):337-345
A cross sectional study was conducted to assess the prevalence of synthetic pyrethroids (SP) resistance in Rhipicephalus (Boophilus) microplus in India. Twenty-seven areas located in six agro-climatic regions were selected for the collection of engorged ticks using two stage stratified sampling procedure. Adult immersion test (AIT) and larval packet test (LPT) were optimized using laboratory reared susceptible line of R.(B.) microplus (IVRI-I) for determination of 95% lethal concentration (LC(95)) of deltamethrin (29.6 ppm in AIT and 35.5 ppm in LPT) and cypermethrin (349.1 ppm in AIT and 350.7 ppm in LPT). The AIT with a discriminating dose (2 × LC(95)) was used to detect deltamethrin and cypermethrin resistance in the field isolates of R.(B.) microplus. On the basis of the data generated on three variables viz., mortality, egg masses and reproductive index, the resistance level was categorized as I, II, III and IV. The overall prevalence of SP-resistant R.(B.) microplus among the sampled farms was 66.6% (18/27). Out of these 18 areas, resistance to deltamethrin at level I was detected in 08 areas (resistance factor=2.0-4.9), at level II in 09 areas (RF=5.2-11.8), at level III in 01 area (RF=34.9) and at level IV in 01 area (RF=95.7). The resistance to cypermethrin was detected in 16 areas and level of resistance was detected at level I in 10 areas (RF=2.06-4.64) and at level II in 06 areas (RF=5.13-9.88). The middle-gangetic and trans-gangetic plains revealed higher density of resistant ticks where intensive cross bred cattle population are reared and the SP compounds are commonly used. The data generated on acaricide resistant status in ticks will help in formulating tick control strategy for the country. 相似文献