排序方式： 共有17条查询结果，搜索用时 15 毫秒
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Carola Gómez‐Rodríguez Carmen Díaz‐Paniagua Javier Bustamante Alexandre Portheault Margarita Florencio 《水产资源保护:海洋与淡水生态系统》2010,20(6):668-677
- 1. Diversity assessments and conservation management should take into account the dynamic nature of populations and communities, particularly when they are subject to highly variable and unpredictable environmental conditions.
- 2. This study evaluates the inter‐annual variability in the assemblage composition (temporal turnover) of an amphibian community breeding in a highly dynamic habitat, a Mediterranean temporary pond system, during a 4‐year period.
- 3. A comprehensive framework is provided to evaluate temporal turnover from data of a differing nature (species richness, presence/absence and relative abundance) and, especially, to discern variation in richness (species loss) from changes in the identity or abundance of species (species replacement).
- 4. Results show that the pond amphibian assemblages in Doñana National Park exhibited high inter‐annual variability during the study period, both in the number of species, species identity and their relative abundance. This result provides evidence for the inadequacy of surveys conducted only in one breeding season to characterize the species assemblage associated with a given pond. Besides, it suggests that a given pond offers different breeding opportunities over time, being suitable for different species depending on the year. This alternation will contribute to the medium‐term preservation of all species in the assemblage.
- 5. It is highly relevant to preserve the natural dynamism and spatial variability of temporary pond systems, which will favour the conservation of populations through their intrinsic variability. Copyright © 2010 John Wiley & Sons, Ltd.
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Esperanza Paniagua Crespo Margarita Marti Mallen Ma P. Monica Penas Ares Ma Cristina Arias Fernandez Ma Pilar Combarro Combarro 《Water, air, and soil pollution》1990,53(1-2):103-111
Tests for free-living amoebae in water from 11 public fountains in Galicia were conducted at two seasons of the year, winter and summer. Only one fountain gave a negative result for the presence of amoebae in both samples. Twenty five strains were isolated, 14 belonging to the genus Acanthamoeba and 10 to the genus Naegleria. The Acanthamoeba strains were made up of the species A. polyphaga, A. quina, A. castellanii and A. paradivionensis. No relation was found between temperature, presence of free chlorine in the water and whether the water was drinkable and the presence or absence of amoebae, nor with the number of strains present in the water samples. The pathogenic capacity of the strains isolated from the genera Naegleria and Acanthamoeba was tested in vivo. It was found that of the 22 strains that would grow at 37 °C, 4 (3 strains of Acanthamoeba and 1 of Naegleria) caused the death of a statistically significant number of mice that had been inoculated intracerebrally, and the presence of amoebae was confirmed in the brains of all the animals inoculated. Intranasal inoculation caused less mice deaths than intracerebral inoculation, and less organs were found containing amoebae. 相似文献
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Vázquez-Prieto S Vilas R Mezo M González-Warleta M Ubeira FM Paniagua E 《Veterinary parasitology》2011,176(1):84-88
Protein electrophoresis was used to study allozyme variation in Fasciola hepatica collected from three locations in Galicia (NW Spain), an area where fascioliasis is endemic. Eleven of 16 loci showed variation in at least one population and 7 loci were polymorphic in all populations studied. Five of these markers showed expected heterozygosities ranging from 0.137 to 0.569. The Nei's unbiased genetic diversity within populations ranged from 0.146 to 0.168. Genotypic frequencies were consistent with panmixia in 25 of 28 cases. Only 2 loci showed a significant deficit of heterozygotes. Genetic distances between populations were small (D(a)=0.003-0.010). These results suggest high levels of genetic variability and low population structure. This study shows that several of the markers developed are useful for study the population genetic structure of the parasite, which is essential to investigate the evolution of drug resistance that has recently emerged in populations of the study area. 相似文献
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Karina Morales‐Ueno Hugo H Montaldo Alfonso Martínez Ortega Carmen G Paniagua‐Chávez Héctor Castillo‐Juárez 《Aquaculture Research》2013,44(8):1254-1258
Some shrimp hatcheries use artificial insemination (AI) to improve the male to female ratio in their breeding populations. We describe a sperm extender solution, which allows the short‐term storage of diluted sperm in Litopenaeus vannamei, and its use in an artificial insemination process. We also evaluate its fertilization capacity. An AI experiment was designed using two, one, or half spermatophore segments. We tested four treatments involving three different male:female ratios: Natural mating (1:1), Regular and Regular diluted (1:2) and Half diluted (1:4). Data analysis revealed that the number of nauplii produced per mating was affected by treatment, with Regular (158 420) performing better than Half diluted (112 864) (P < 0.05), but with no differences between the latter and Regular diluted (130 340) (P > 0.05). A binomial variable named female success (FS) was defined as successful when the number of nauplii obtained per mate was ≥25 000. Analysis showed differences for FS across treatments (P < 0.001), but not between Regular (79.2%), the hatchery conventional AI technique and Half diluted (60.4%), maybe due to sample size. Since the number of nauplii per mate is crucial to consider AI successful, it is necessary to improve this AI technique before it can be used in the shrimp industry. 相似文献
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Martha Reyes‐Becerril Felipe Ascencio‐Valle José Meseguer Silvana Teresa Tapia‐Paniagua Miguel Angel Moriñigo María Ángeles Esteban 《Aquaculture Research》2012,43(8):1107-1118
This study evaluates the effects of the dietary administration of the live yeast Debaryomyces hansenii strain L2 on the immune responses of gilthead seabream for 4 weeks. Cellular immune parameters were measured from serum and head‐kidney leucocytes respectively. The expression levels of immune‐associated genes were quantified by real‐time polymerase chain reaction. In addition, the profile of intestinal microbiota was studied by denaturing gradient gel electrophoresis. The results showed that seabream fed a diet containing D. hansenii had significantly increased cellular immune parameters. The yeast‐supplemented diet up‐regulated the expression of most seabream genes at week 2 and down‐regulated all of them at week 4, except in the head‐kidney. Finally, a reduction in the diversity of the intestinal microbiota was detected in those specimens receiving the yeast‐supplemented diet. These results support the idea that the live yeast D. hansenii strain L2 stimulates the immune system of gilthead seabream. 相似文献
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C Arriaga de Morilla R Paniagua A Ruíz-Navarrete C R Bautista A Morilla 《Veterinary parasitology》1989,30(3):197-203
The dot enzyme-linked immunosorbent assay (Dot-ELISA) was compared with the passive haemagglutination test (PHT) and thin layer immunoassay (TIA) for the detection of antibodies against Fasciola hepatica in naturally and experimentally infected sheep. The infected animals gave titres from 1:25,000 to 1:204,000, while control animals gave titres of from 1:100 to 1:800. The titres of the infected sheep obtained by Dot-ELISA were 1000-2000 times higher than the ones obtained using TIA or PHT. Due to its high sensitivity, this technique could be very useful for the diagnosis of ovine fascioliasis. 相似文献
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An amoeba associated with a severe gill disease affecting farmed turbot, characterized by pronounced hyperplasia of the branchial epithelia and accumulation of mucus between the secondary lamellae, is described for the first time. The amoeba was isolated, cloned and cultured on Bactoagar. Light microscopy showed that trophozoites had a mean length of 12 μm (range 8–20 μm), with a nucleus 3–5 μm in diameter containing a large central nucleolus 1–3 μm in diameter. Transmission electron microscopy showed that the mitochondria, Golgi bodies, rough endoplasmic reticulum, microfilaments, cytoplasmic microtubules and glycocalyx were similar to those previously reported for the genus Platyamoeba. These and other findings strongly suggest that the amoeba isolated is of the genus Platyamoeba, while its morphometric characteristics suggest that it may represent a new species. This is the first report of a species of the genus Platyamoeba in association with gill disease in turbot. 相似文献
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Irrigation scheduling results from the irrigator's integration of meteorological, environmental and crop information. In this paper, the irrigation scheduling patterns of a group of irrigators in the Candasnos Water Users Association (WUA), located in north-eastern Spain, were analysed. Scheduling sprinkler and drip irrigation in this WUA shows additional complications due to the sharing of a collective pressurized irrigation network and to the need to file water orders two days in advance of its foreseen use. The database created by a remote surveillance and control system was mined to obtain the time evolution of hydrant operation time during the 2004–2008 irrigation seasons. Records were selected for clearly identified crops and irrigation systems, and for verified water allocations. Hydrant operation showed a relationship with meteorology (precipitation, wind speed, relative humidity and air temperature), although this relationship was often not evident when hydrants were individually analysed. Statistical analyses were run to classify irrigator's scheduling practices, leading to the establishment of ten different groups. The adopted classification criteria included the average number of weekly irrigations, the SD of the number of weekly irrigations and the modal range of the irrigation starting time. The irrigation pattern was determined by the irrigator (56%), the irrigation system (33%), and the crop (11%). Only in a fraction of the cases (22%) the time change in the scheduling pattern responded to a clear time trend; in 39% of the cases, changes in time appeared random. Further, 45% of the irrigators used the same irrigation pattern in at least half of their hydrant-years, independently of the crop. Only 14% of the irrigators applied different irrigation scheduling patterns to different crops. Our results suggest that irrigators do not find value or do not have the capacity to develop irrigation patterns more consistent and adapted to the local environment, the crops and the irrigation systems. 相似文献