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Effects of dietary supplementation with β‐glucan and synbiotics on growth,haemolymph chemistry,and intestinal microbiota and morphology in the Pacific white shrimp 
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下载免费PDF全文 S. Boonanuntanasarn U. Wongsasak T. Pitaksong S. Chaijamrus 《Aquaculture Nutrition》2016,22(4):837-845
The goal of this study was to investigate the effects of dietary supplementation with β‐glucan and microencapsulated probiotics (Bacillus subtilis or Pediococcus acidilactici) on growth performance, body composition, haemolymph constituents, and intestinal morphology and microbiota of the Pacific white shrimp Litopenaeus vannamei. Four treatment diets [basal diet (C), β‐glucan‐containing diet (β‐glu), β‐glucan plus B. subtilis‐containing diet (β‐glu+Bs), and β‐glucan plus P. acidilactici‐containing diet (β‐glu+Pa)] were fed to L. vannamei for 90 days. Shrimp fed the β‐glu and β‐glu+Pa diets exhibited similar growth performance and body protein content, which were significantly higher than those of shrimp fed the control diet (P < 0.05). No significant differences in haemolymph triglyceride, cholesterol, protein, haemolymph urea nitrogen or chloride were detected among the experimental diets. However, dietary β‐glucan alone increased the haemolymph glucose level and osmolarity (P < 0.05). Synbiotic supplementation had greater effects on intestinal microbiota and morphology than dietary β‐glucan alone. For example, β‐glu+Bs increased the number of intestinal lactic acid bacteria and decreased the number of Vibrio spp. (P < 0.05), and β‐glu+Pa increased the height of intestinal villi. 相似文献
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Surintorn Boonanuntanasarn Pranorm Khaomek Taratip Pitaksong Yanling Hua 《Aquaculture International》2014,22(4):1417-1436
This study shows the effects of dietary activated charcoal (AC) on health status, intestinal morphology and fillet geosmin content of Nile tilapia prior to harvesting (2 and 4 weeks). Four dietary treatments (each diet in six replicates) were formulated to incorporate AC at levels of 0, 10, 20 and 30 g kg?1 of the dry diet. Fish were reared in hapas, which were located in earthen ponds. There were not significant differences in growth performances among experimental treatments. The moisture and protein content in the fillet decreased and increased, respectively, as the incorporation level of AC increased. The hematological indices and several immune parameters did not differ significantly among treatment groups. Among the fifteen blood chemicals parameters examined, the significant reductions in protein and cholesterol and the changes in blood minerals were observed in fish fed dietary AC ≥20 g kg?1. Dietary AC tended to increase the height of intestinal villi and goblet cell number. Dietary AC also influenced the reduction in geosmin in the fish fillet. Taken together, these findings indicate that AC (at 10 g kg?1 diet) could be used as feed supplement for Nile tilapia prior harvesting to reduce geosmin without negative effects. 相似文献
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So far, there are a number of fish genome projects, including experimental and economically important fish that provide available DNA sequence information. However, the function of a gene cannot be deduced only by its DNA sequence. Therefore, a technique with which to investigate the function of the fish gene is needed. Gene knockdown (GKD), or antisense technology, is now being used as a powerful technique to study gene functions in living organisms. GKD effects result from the introduction of an antisense molecule into living cells. The antisense agents bind to target messenger RNA, thus inactivating the target gene expression. The appropriately spatial inhibitory effects on protein production from corresponding gene resulted in the phenotypic change. Therefore, the function of the gene can be understood. To date, there are a number of antisense molecules that can affect efficient GKD in fish. These include antisense oligonucleotides, small interfering RNA, and ribozyme. These antisense molecules cause specific gene inhibitor effects with different mechanisms. The various antisense mechanism types facilitate a number of GKD applications with various approaches in animals. In this review, we demonstrate the characteristics of each antisense molecule, its mechanism, and its application, especially for gene functional analysis in fish. 相似文献
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This study investigated the effects of the co‐supplementation of vitamins C (0, 500, and 1000 mg kg?1) and E (0, 62.5, and 125 mg kg?1) on the growth performance, haematology and the modulation of blood stress indicators and immune parameters in hybrid catfish (Clarias macrocephalus × Clarias gariepinus) under combinations of thermal and acidic stress. Supplementation of vitamins C and E influenced the growth, haematological indices, serum chloride, plasma protein and immune parameters (lysozyme, total immunoglobulin and alternative complement haemolytic assay) (P < 0.05). Although vitamins C and E did not prevent a significant reduction in serum chloride, they minimized not only the modulation of blood glucose and plasma protein, but also the reduction in immune parameters (P < 0.05) owing to stress. Our results demonstrated that co‐supplementation of 500 mg kg?1 vitamin C and 125 mg kg?1 vitamin E, or 1000 mg kg?1 vitamin C alone, for four weeks and co‐supplementation of both vitamins at low levels (vitamins C at 500 mg kg?1 and E at 62.5 mg kg?1) for eight weeks had beneficial effects on the growth, amelioration of stress‐mediated adverse changes in the physiological and immunosuppressive responses of hybrid catfish under stressful conditions. 相似文献
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不同数量猪毛中提取基因组DNA的比较 总被引：1，自引：0，他引：1
杨胜林 《山地农业生物学报》2005,24(5):457-459
在进行分子生物学试验尤其是动物分子标记的研究中需要大量纯化的基因组DNA,这些DNA通常情况下是从动物的血液、耳组织、肝脏等组织中提取,所提取的基因组DNA质量较好.但是,在进行本地猪遗传多样性研究过程中,通常需要大量的血样,其获得和收集较为困难,尤其是野猪的血样更不易获得.因而,从猪的毛发中提取合格的DNA将解决上述难题.通常认为,从动物的毛发中提取DNA无论从数量还是质量上均明显低于从血样或其他组织中提取的DNA .但也有从脱落的狗毛中成功地提取基因组DNA的报道.为了探索从毛发中提取合格DNA的途径,本试验通过从不同数量的猪毛中提取的DNA进行质量比较分析,找出适合于做分子标记试验所需猪毛样本数量,为提高样本收集效率,降低劳动强度和减低试验成本提供依据. 相似文献
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Effects of dietary inulin and Jerusalem artichoke (Helianthus tuberosus) on intestinal microbiota community and morphology of Nile tilapia (Oreochromis niloticus) fingerlings 下载免费PDF全文
下载免费PDF全文 S. Boonanuntanasarn N. Tiengtam T. Pitaksong P. Piromyou N. Teaumroong 《Aquaculture Nutrition》2018,24(2):712-722
This study investigated the effects of dietary inulin and Jerusalem artichoke (JA) on intestinal microbiota and morphometry of Nile tilapia fingerlings. Five treatment diets were designed to supplement inulin at 0 (basal diet), 2.5 and 5.0 g/kg, and JA at 5.0 and 10.0 g/kg. Nile tilapia larvae were fed experimental diets from the first feeding through the fingerling stage (84 days). The cultivation‐dependent technique showed that dietary inulin at 5.0 g/kg and JA (at both levels) increased lactic acid bacteria and Bifidobacterium spp., but decreased Vibrio spp. (p < .05). PCR‐DGGE targeting 16S ribosomal RNA gene revealed that dietary inulin and JA generated different profiles of microbial community compared with fish fed a basal diet. Compared with fish fed the basal diet, a greater intestinal villi height was observed in fish fed 5.0 g/kg inulin and JA at both levels (p < .05). A larger relative goblet cell number were observed in the anterior intestine of fish fed 5.0 g/kg inulin or JA (p < .05). Overall, dietary inulin (5.0 g/kg) and JA (5 and 10.0 g/kg) since the first feeding had effects on modulating the intestinal microbiota and morphology of Nile tilapia fingerlings. 相似文献
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The effects of dietary inulin and Jerusalem artichoke (Helianthus tuberosus) tuber on the growth performance,haematological, blood chemical and immune parameters of Nile tilapia (Oreochromis niloticus) fingerlings 下载免费PDF全文
下载免费PDF全文 Nattanan Tiengtam Pramote Paengkoum Suthida Sirivoharn Khanakorn Phonsiri Surintorn Boonanuntanasarn 《Aquaculture Research》2017,48(10):5280-5288
This study investigated the effects of dietary inulin or Jerusalem artichoke (JA) on the growth performance, haematological, blood chemical and immune parameters of Nile tilapia fingerlings. Five treatment diets were designed to incorporate inulin at 0 (basal diet), 2.5 and 5.0 g/kg and JA at 5.0 and 10.0 g/kg. Two basal diets including fish meal and formulated experimental feed were used for fry and fingerling growing periods, respectively. During the fry growing period, larvae were fed treatment diets for 4 weeks. There were no significant differences in growth performance or survival rate. Fingerlings were then nursed with the formulated experimental diets from weeks 5 to 12. Fingerlings fed on inulin at 5.0 g/kg or JA at either level had better growth performance and survival rate than that fed on the basal diets. There were no significant differences in body composition. Dietary prebiotic inulin and JA increased red blood cell number (p < .05). Among the five blood chemistry parameters examined, both inulin at 5.0 g kg and JA (5.0 and 10.0 g/kg) increased blood protein (p < .05). Dietary inulin at 5.0 g/kg and JA at 5.0 and 10.0 g/kg increased total immunoglobulin and lysozyme activity (p < .05). Both inulin and JA inclusion diets increased alternative complement activity (p < .05). Taken together, dietary inulin at 5 g/kg and JA at 5.0 and 10.0 g/kg had beneficial effects on the growth performance, survival rate and immune of Nile tilapia fingerlings. 相似文献
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