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1.
The purpose of this experiment was to characterize the species of Eimeria affecting lambs and their infection pattern. Faecal samples were collected from each animal at 14-day intervals, beginning when lambs were 2 weeks old and ending when they were 32 weeks old. The oocysts were counted and identified as E. intricata, E. parva, E. pallida, E. crandallis, E. bakuensis, E. weybridgensis, E. ahsata and E. ovinoidalis. The highest oocyst counts were observed when the lambs were 4-8 weeks old. 相似文献
2.
Silva BF Bassetto CC Shaw RJ Canavessi AM Amarante AF 《Veterinary parasitology》2012,186(3-4):437-444
Previous studies showed that Santa Ines (SI) hair sheep were more resistant to gastrointestinal nematode infections (GIN) than Ile de France (IF) sheep. The present experiment aimed to evaluate if that reported resistance difference against GIN also occurred against Oestrus ovis infestation and also to evaluate the influence of O. ovis infestation on the gastrointestinal nematodes (GIN) infections. SI (n=12) and IF (n=12) young male lambs were weaned at 2 months of age and moved to a paddock (0.3 ha) with Brachiaria decumbens grass, where they also received concentrate ration. The animals were kept together during the experimental period (September to early December 2009). Fecal and blood samples were taken from all animals every 2 weeks and body weight and nasal discharge score (oestrosis clinic signs) were recorded on the same occasion. In early December 2009, all lambs were sacrificed and O. ovis larvae and GIN were recovered, counted and identified according to the larval stage. All animals were infested by different larval instars of O. ovis without any statistical difference between breeds (P>0.05). The SI lambs had an average of 24.8 larvae, and the intensity of infection ranged between 14 and 39 larvae, while the IF lambs showed an average of 23.5 larvae with the minimum and maximum from 11 to 36 larvae, respectively. SI lambs presented the lowest nematode fecal egg counts (FECs) and the lowest mean numbers of Haemonchus contortus, Trichostrongylus colubriformis and Strongyloides papillosus, however, there was no significant differences between group means (P>0.05). Inverse relationship between numbers of O. ovis larvae and gastrointestinal nematodes was observed in both breeds. SI sheep showed a significant increase in blood eosinophils and total IgE serum levels and these variables were negatively correlated with nematode FEC. A negative correlation was observed between total IgE serum level and H. contortus burden in both breeds. In conclusion, there was no breed difference regarding O. ovis infestation and in each breed, animals with more nasal bot fly larvae tended to display smaller worm burden. 相似文献
3.
Pearce PD Ansari HA Maher DW Amarante MR Monro TL Hendrikse WL 《New Zealand veterinary journal》1997,45(2):69-71
A Robertsonian centric fusion between chromosomes 1 and 25 in Blonde d'Aquitaine cattle in New Zealand is reported. This fused chromosome is the same as the widely reported 1/29 translocation chromosome with the difference in the numbering arising from inconsistencies in the G and R-banded cattle karyotypes of the International System for Cytogenetic Nomenclature of Domestic Animals, 1989. 相似文献
4.
Bricarello PA Zaros LG Coutinho LL Rocha RA Silva MB Kooyman FN De Vries E Yatsuda AP Amarante AF 《Veterinary parasitology》2008,155(1-2):95-103
Cellular and humoral immune response, as well as cytokine gene expression, was assessed in Nelore cattle with different degrees of resistance to Cooperia punctata natural infection. One hundred cattle (male, weaned, 11-12 months old), kept together on pasture, were evaluated. Faecal and blood samples were collected for parasitological and immunological assays. Based on nematode faecal egg counts (FEC) and worm burden, the seven most resistant and the eight most susceptible animals were selected. Tissue samples of the small intestine were collected for histological quantification of inflammatory cells and analysis of cytokine gene expression (IL-2, IL-4, IL-8, IL-12p35, IL-13, TNF-alpha, IFN-gamma, MCP-1, MCP-2, and MUC-1) using real-time RT-PCR. Mucus samples were also collected for IgA levels determination. Serum IgG1 mean levels against C. punctata antigens were higher in the resistant group, but significant differences between groups were only observed 14 days after the beginning of the experiment against infective larvae (L3) and 14 and 84 days against adult antigens. The resistant group also presented higher IgA levels against C. punctata (L3 and adult) antigens with significant difference 14 days after the beginning of the trial (P<0.05). In the small-intestine mucosa, levels of IgA anti-L3 and anti-adult C. punctata were higher in the resistant group, compared with the susceptible group (P<0.05). Gene expression of both T(H)2 cytokines (IL-4 and IL-13) in the resistant group and T(H)1 cytokines (IL-2, IL-12p35, IFN-gamma and MCP-1) in the susceptible group was up-regulated. Such results suggested that immune response to C. punctata was probably mediated by T(H)2 cytokines in the resistant group and by T(H)1 cytokines in the susceptible group. 相似文献
5.
A. F. T. Amarante M. A. Barbosa T. C. Oliveira-Sequeira S. Fernandes 《Tropical animal health and production》1992,24(1):36-39
Summary A survey of Diptera species causing cutaneous myiases on sheep in Botucatu, S?o Paulo, Brazil was made to determine seasonal
incidence, predilection sites and the factors predisposing to infestation. Sheep were checked daily for myiases for one year.
At two week intervals larvae from wounds were collected for identification. Only larvae ofDermatobia hominis andCochliomyia hominivorax were found. Myiases due toC. hominivorax were observed during the whole year with high incidence from January to April. The feet, vulva, tail and scrotum were most
frequently infested. Wounds were the commonest predisposing factor.
Resumen Se realizó un estudio de la especieDiptera involucrada en miasis cutánea en ovejas en Botucatu, Sao Paulo, Brasil, con el fin de determinar la incidencia estacional, sitios de predilección y todos los factores predisponentes a la infestación. Las ovejas se examinaron diariamente por miasis, durante un a?o, colectandose larvas de las heridas con intervalos de dos semanas, para la identificación. Se encontraron larvas deDermatobia hominis yCochliomvia hominivorax únicamente. Las miasis causadas porC. hominivorax se observaron durante todo el a?o, con la incidencia más alta desde enero hasta abril. Las patas, vulvas, cola y escroto fueron los sitios predilectos para la infección, siendo las heridas el factor más predisponente.
Résumé Une étude portant sur les espèces de Diptères à l'origine des myiases cutanées chez le mouton à Botucatu, Brésil, a éte effectuée pour déterminer l'incidence saisonnière, les sites de prédilection et les facteurs prédisposant à l'infestation. Pendant un an, des moutons susceptibles d'être atteints de myiases ont été surveillés quotidiennement. A 2 semaines d'intervalle, des larves provenant de blessures ont été prélevées pour identification. On a seulement trouve des larves deDermatobia hominis et deCochliomyia hominivorax. Les nymphes attribuables àC. hominivorax ont été observées pendant toute l'année, avec une incidence élevée de janvier à avril. Les pattes, la vulve, la queue et le scrotum étaient les plus fréquemment infestés. Les blessures étaient les facteurs de risque les plus répandus.相似文献
6.
S.M. Haefele S.M.A. Jabbar J.D.L.C. Siopongco A. Tirol-Padre S.T. Amarante P.C. Sta Cruz W.C. Cosico 《Field Crops Research》2008
Water and nutrient availability are two major constraints in most rice-based rainfed shallow lowland systems of Asia. Both stresses interact and contribute to the low productivity and widespread poverty in this environment. The objective of this study was to improve the understanding of interaction between the two factors and to identify varietal characteristics beneficial for productivity in a water- and nutrient-limited rice environment. For this purpose, we screened 19 rice genotypes adapted to different rice environments under two water and two nutrient treatments during the wet season of 2004 and 2005 in southern Luzon, Philippines. Across all genotypes tested and in comparison with the irrigated control, rainfed conditions reduced grain yield of the treatment without N application by 69% in 2004 and by 59% in 2005. The mean nitrogen fertilizer response was highest in the dryer season of 2004 and the rainfed treatment, indicating that water stress had no effect on fertilizer response. Nitrogen application reduced the relative yield loss to 49% of the irrigated treatment in 2004 and to 52% of the irrigated treatment in 2005. Internal efficiency of N (IEN) and recovery efficiency of applied N (REN) were significantly different between genotypes, but were not affected by water availability (REN) or by water and nutrient availability (IEN). In contrast, grain yield and total N uptake were affected by cultivar, N and water availability. Therefore, germplasm for rainfed environments should be screened under conditions of limited and good nitrogen and water supplies. The four best cultivars, CT6510-24-1-2, IR55423-01, IR72, and IR57514-PMI5-B-1-2, performed well across all treatments and both years. Except for IR72, they were all characterized by medium height, medium duration, high early vigor, and a moderate level of drought tolerance. This combination of characteristics seems to enable the optimal use of limited water and nutrient resources occurring in many shallow rainfed lowlands. We also concluded that moderate drought stress does not necessarily affect the response to moderate N rates, provided that drought does not induce high spikelet sterility and that fertilizer N is properly managed. 相似文献
7.
A F Amarante T M Craig W S Ramsey N M El-Sayed A Y Desouki F W Bazer 《Veterinary parasitology》1999,85(1):61-69
The response to natural infection by gastrointestinal nematodes was evaluated in three groups of lambing mature ewes: 16 Florida Native; 21 Rambouillet; and 18 1/2 Florida Native x1/2 Rambouillet (F1 generation). Fecal egg counts (FEC), packed cell volume (PCV) and plasma protein levels were determined periodically through a one-year period. The highest FEC was recorded in Rambouillet ewes, with most of the means higher than 1000 eggs per gram (EPG). Florida Native and F1 ewes showed similar trends in FEC, with most of the means below 500 EPG. The highest mean PCV values were recorded in Florida Native ewes, followed in decreasing order by F1 and Rambouillet ewes. F1 ewes showed the lowest plasma protein levels and Florida Natives the highest. Fecal cultures revealed the highest percentages of Haemonchus larvae in Rambouillet ewes (>64%) and the lowest percentages in Florida Native ewes. Florida Native and F1 ewes were more resistant to gastrointestinal nematode infections than were Rambouillet ewes. High negative correlation coefficients between FEC x PCV and FEC x plasma protein levels coincided with peaks in mean FEC of Rambouillet (May and July), Florida Native and F1 ewes (October). This study showed that crossbreeding Rambouillet and Florida Native sheep resulted in the production of F1 ewes resistant against gastrointestinal nematode infections. 相似文献
8.
A. F. T. Amarante T. M. Craig W. S. Ramsey S. K. Davis F. W. Bazer 《Veterinary parasitology》1999,80(4):277-324
This experiment was carried out to compare the worm burden and cellular responses in the abomasal mucosa and blood of Florida Native and Rambouillet lambs and also in animals produced by crosses of these two breeds (generations F1 and F2). Animals were exposed to infection by gastrointestinal nematodes on three different occasions. The first infection was natural, occurring while they were suckling lambs. After weaning, they were kept indoors for 53 days and then were allowed to graze a contaminated pasture for 50 days for a second natural infection. The third infection was an artificial challenge with 6000 Haemonchus contortus infective larvae. The highest mean fecal egg counts (FEC) values were found in Rambouillet lambs followed in decreasing order by F1, F2 and Florida Native lambs. Throughout the trial, most of the high mean packed cell volumes and plasma protein levels were recorded in the F2 lambs; in contrast, most of the low values were found in the Rambouillet lambs. During the natural infection period, the highest percentages of larvae in the fecal cultures of the lambs were Haemonchus. However, high percentages of Trichostrongylus larvae were found particularly in Florida Native lambs. The mean number of blood eosinophils increased after the artificial challenge, reached a peak 21 days after infection and then declined. The highest and lowest blood eosinophil means were recorded in F2 and Florida Native lambs, respectively. The H. contortus burden was significantly higher in Rambouillet and in F1 lambs than in Florida Native and F2 lambs (p < 0.05), while no significant differences were recorded among eosinophil, mast cell and globule leucocyte counts in the abomasal mucosa (p > 0.05). The highest correlation coefficient recorded at the end of this study was between FEC and worm burden (r = 0.7). These two parameters showed a moderate negative correlation with PCV, plasma protein and mast cell counts in the abomasal mucosa. The results obtained in this study indicate that crossbreeding Florida Native and Rambouillet sheep can be a rapid way to combine and improve the characteristics of these two breeds. The parasitological results were promising, however, more studies will be necessary to verify the impact of crossbreeding in other traits. 相似文献
9.
Rocha RA Bricarello PA Silva MB Houdijk JG Almeida FA Cardia DF Amarante AF 《Veterinary parasitology》2011,181(2-4):229-238
This experiment aimed to evaluate the effect of periparturient metabolizable protein (MP) nutrition on resistance to Haemonchus contortus in single rearing Ile de France and Santa Ines ewes. The restriction-fed iso-energetic diet was calculated to provide either 0.8 (low MP diet) or 1.3 (high MP diet) times MP, from three weeks before parturition until eight weeks into lactation. The ewes were experimentally infected with 1000 H. contortus infective larvae (L3) three times a week (Mondays, Wednesdays and Fridays), starting five weeks before the predicted date for parturition until a total of 15,000 L3 had been administered. While both breeds showed elevated fecal egg counts (FEC), these values were significantly lower for Santa Ines ewes than Ile de France ewes, but were independent of level of MP feeding. The latter also did not affect lamb weight gain and ewe body weight variation in each breed. Packed cell volume and total plasma protein for Santa Ines in all periods were significantly higher than those for Ile de France ewes (P<0.01) but were not affected by nutrition. In contrast, levels of serum IgG and IgA antibodies against somatic H. contortus infective larvae and adult antigens were similar between breeds but higher in animals that received high MP diets (P<0.05). The reduced body score of ewes at the beginning of the experiment probably influenced their high susceptibility to incoming larvae. Since, unexpectedly, MP scarcity was not achieved in this experiment, our data support the view that Santa Ines ewes are more resistant to H. contortus than Ile de France ewes. 相似文献
10.
Cassandro Vidal Talamini do Amarante Cristiano André Steffens Luiz Carlos Argenta 《Scientia Horticulturae》2011
Covering apple orchards with nets protects them from hailstorms but this changes the amount and quality of the light supplied to the trees. This study was carried out to assess the effects of shade provided by white protection net on yield and fruit quality of apple orchards in Southern Brazil, along three growing seasons (2002/2003, 2003/2004, and 2004/2005). ‘Gala’ and ‘Fuji’ apple trees (on MM111 rootstock, raised as slender spindles, with a spacing of 5.5 m × 3 m) were left uncovered (control trees) or covered with white net. The white net reduced the photosynthetically active radiation (λ = 400–700 nm) accumulated over the trees’ canopy along a day by 18.4%, leading to a higher leaf chlorophyll content in both cultivars, and a higher specific leaf area in ‘Gala’. The shade provided by the net did not affect yield and increased fruit average weight in both cultivars. At harvest, the number of seeds per fruit and severity of russet were not affected by netting in both cultivars, as well as the incidence of watercore in ‘Fuji’. The main beneficial effects of the white net were the reduced incidence of sunburn on ‘Gala’, reduced incidence of bitter pit during cold storage in both cultivars, and reduced incidence of apple scab at harvest and of decay after cold storage in ‘Fuji’. Fruit fly damage in ‘Gala’ (assessed in one season) was reduced by netting. However, netting affected negatively some fruit quality attributes. ‘Gala’ and ‘Fuji’ apples from trees protected by the net had a poor skin color (pale blush and a more intense green background color) at harvest. The net reduced flesh firmness and SSC, and increased the starch index of ‘Gala’ apples at harvest, and reduced flesh firmness and SSC, assessed after cold storage and shelf life, in fruits of both cultivars. 相似文献