Effects of phosphodiesterase inhibitor-induced insulin resistance on glucose and lipid metabolism in rats     

YANG Gang-yi  LI Ling  Peter Chung  Gangther Boden 《园艺学报》2003,19(7):875-880

AIM:To investigate the effects of milrinone (a selective phosphdiesterase III inhibitor PDE₃) on insulin secretion, blood glucose, plasma free fatty acids (FFA) and dose-response relationship, and assess possible effects of milrinone on glucose metabolism and insulin sensitivity in conscious rats.METHODS:The catheterized nonstressed rats were administered by the varying doses of milrinone (1, 5, 25 μmol/kg) and were compared with controls. A hyperinsulinaemic- euglycaemic clamp was established in awake rats, and milrinone(25 μmol/kg) and 25% dimethyl sulfoxide (DMSO, as a control) were given at 120 min during hyperinsulinaemic- euglycaemic clamp. Glucose turnover was decided by gas chromatograph mass spectrometer (GC-MS).RESULTS:After dosing, plasma FFA levels in 3 milrinone groups significantly increased compared with the controls and before dosing. The percentages of elevation of FFA by the different milrinone doses were very similar, 50%, 52%, 55% for 1, 5, 25 μmol/kg respectively at 2 min after dosing. Plasma insulin levels were significantly elevated in the 5 and 25 μmol/kg groups, and the effect of milrinone on glucose concentration was detectable only 25 μmol/kg group. During hyperinsulinaemic clamp, there were significant increase in plasma FFA (from 173.1±15.2 to 633.8±87.3 μEq/L) and hepatic glucose production (HGP), and a significant decrease in glucose infusion rates (GIR) (to about 21%).CONCLUSION:These data suggest that milrinone impaires the abilities of insulin to suppress lipolysis and HGP, and insulin-mediated glucose utilization in peripheral tissue. Therefore, milrinone administration may induce an acute insulin resistancein vivo.  相似文献   

Geographical cline of DNA variation within the F intersterility group of Heterobasidion annosum in Italy     

N. LA PORTA  P. CAPRETTI  K. KAMMIOVIRTA  R. KARJALAINEN  & K. KORHONEN 《Plant pathology》1997,46(5):773-784

Eighty-six Heterobasidion annosum isolates, mainly belonging to the F intersterility group and obtained from 32 different geographical localities in Italy, were subjected to genetic analysis by the Random Amplified Polymorphic DNA (RAPD) markers. The similarity between F and S groups was higher than that between F and P. In UPGMA Cluster Analysis, the F isolates originating from the same locality usually grouped in the same cluster. The isolates also showed a tendency to group at the level of larger geographical areas. Within the F group, isolates from the south of the Italian peninsula showed the highest genetic variation and northern isolates from the Alpine regions showed the lowest. This indicates a gradual cline along the peninsula. The genetic variability in the Italian F group is discussed in relation to the past and present distribution of the host species in Italy and Europe.  相似文献   

Laryngeal mask airway and transient hypercapnic hyperpnea for video‐endoscopic assessment of unilateral laryngeal paralysis in dogs          下载免费PDF全文

Daniel M. Sakai MV  DACVAA  Manuel Martin‐Flores MV  DACVAA  Amanda K. Jones PhD  Galina M. Hayes BVSc  PhD  DACVECC  DACVS‐SA  Marina J. McConkey DVM  DACVS‐SA  Jonathan Cheetham VetMB  PhD  DACVS‐LA 《Veterinary surgery : VS》2018,47(4):543-548

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Risk factors for Thoroughbred racehorse fatality in jump starts in Victoria, Australia (1989-2004)     

Boden LA  Anderson GA  Charles JA  Morgan KL  Morton JM  Parkin TD  Clarke AF  Slocombe RF 《Equine veterinary journal》2007,39(5):422-428

REASONS FOR PERFORMING STUDY: The risk of fatality is greater in jump than in flat racing in Victoria, Australia. This is the first study to identify risk factors specific to jump starts in Victoria. OBJECTIVE: To identify risk factors for fatality of Thoroughbred racehorses in jump starts on all racecourses in Victoria, Australia between 1989 and 2004. METHODS: Fatalities comprised all horses that died during or immediately after a jump (hurdle or steeplechase) race or official jump trial and all horses that were subjected to euthanasia within 24 h of an event in which an injury was sustained. The retrospective study involved 191 case starts and 2324 control starts. Univariable and multivariable backward stepwise logistic regression was used to identify risk factors for fatality at any one start. A multiple level model was used with racecourse included as a random effect. RESULTS: In the final multivariable model, the duration of the racing career of the horse, the number of flat, hurdle and steeple starts accumulated in the 60 days prior to the case or control start, the number of flat and jump starts accumulated over the racing career, if the horse had had a start between 1 and 14 days prior to the case or control start, the type of jump race (hurdle or steeple), the calendar year of the start and the location of the racecourse were associated with fatality. CONCLUSIONS: The findings highlight the need to investigate further the differences between hurdle and steeplechase events and the adverse effect of prolonged prior flat racing careers on the risk of fatality in jump starts. POTENTIAL RELEVANCE: This is the first study to examine risk factors for fatality in jump starts in Victoria. The results should shape the development of interventions to reduce the risk in jump starts in the future.  相似文献   

Serotyping of isolates of Pasteurella multocida from chickens     

LA IRELAND  AR MILNER  IJ SMART 《Australian veterinary journal》1989,66(4):119-120

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不同处理方法对美国截叶胡枝子种子发芽的影响     

拉旦  欧阳克蕙  王堃 《草业与畜牧》2006,(4):14-16

美国截叶胡枝子的硬实率很高,发芽率只有17.3%.经过种子预处理,如采用不同温度下浸种、98%浓硫酸浸泡、机械破皮和机械破皮结合热水浸种等处理,结果表明,机械破皮后用60℃热水浸种处理的效果最好,发芽势和发芽率分别达到64.0%和74.3%;其次是机械破皮,平均发芽率达到50%;浓硫酸浸种可以显著提高种子发芽势,浓硫酸浸种5min效果最好,发芽率达到44.7%;热水浸种的处理效果不佳,最好的90℃热水浸种处理发芽率也仅有26.7%.  相似文献   

不同处理方法对美国截叶胡枝子种子发芽的影响   总被引：1，自引：0，他引：1  

拉旦  欧阳克蕙  王堃 《黑龙江畜牧兽医》2006,(8):57-58

截叶胡枝子(Lespedezacunecta)是原产于亚洲东部的一种多年生的豆科植物,在我国南方地区野生分布很广。它具有耐瘠、耐旱、耐酸、耐热、固土能力强、适应性广等特性,在国外也颇受重视。我国于1983年从美国引种,通过对其生物学和生态学特性、经济性状和栽培技术研究证明,引进的美国截叶胡枝子在人工栽培条件下,表现出良好的经济性状,是南方草地改良的希望种子。但是在生产中,由于其硬实的存在,使得种子的发芽率很低,往往造成出苗困难和草地建植缓慢,甚至出现严重缺苗断垄的现象,直接影响田间产量,限制了它的进一步推广应用。试验采用不同处…  相似文献   

不同处理方法对美国截叶胡枝子种子发芽的影响   总被引：2，自引：0，他引：2  

拉旦  欧阳克蕙  王堃 《四川草原》2006,(4):14-16

美国截叶胡枝子的硬实率很高,发芽率只有17.3%。经过种子预处理,如采用不同温度下浸种、98%浓硫酸浸泡、机械破皮和机械破皮结合热水浸种等处理,结果表明,机械破皮后用60℃热水浸种处理的效果最好,发芽势和发芽率分别达到64.0%和74.3%;其次是机械破皮,平均发芽率达到50%;浓硫酸浸种可以显著提高种子发芽势,浓硫酸浸种5min效果最好,发芽率达到44.7%;热水浸种的处理效果不佳,最好的90℃热水浸种处理发芽率也仅有26.7%。  相似文献   

Effects of Sodium Dodecyl Sulphate on Post-thaw Dog Semen Quality during in vitro Incubation at 39°C and 22°C     

AI Peña  F Barrio  LA Quintela  PG Herradón 《Reproduction in domestic animals》1998,33(6):393-398

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不同退化程度高寒草甸植物物种多样性与生态系统多功能性关系     

周宸宇  杨晓渊  邵新庆  张中华  马丽  秦瑞敏  佘延娣  苏洪烨  常涛  魏晶晶  胡雪  拉本  周华坤 《草地学报》2022,30(12):3410-3422

为探究高寒草甸不同退化程度生态系统多功能性的变化,本研究综合考虑了植物物种多样性和多种生态系统功能如生产力、养分循环和固存能力、土壤涵养水分能力等,运用降维因子分析的方法计算了生态系统多功能性,综合分析了植物物种多样性与生态系统功能和多功能性间的关系。结果表明：高寒草甸退化显著降低了Margalef指数、Shannon-weiner指数、Simpson指数和Pielou指数(P<0.05),各生态系统功能趋于恶化。高寒草甸退化导致生态系统多功能性的下降,综合统计量结果为轻度退化(21.9955)＞中度退化(8.7295)＞重度退化(—30.7245)。植物物种多样性与生态系统多功能性之间均存在正相关关系,其中Simpson指数与生态系统多功能性指数拟合的结果最好,说明高寒草甸生态系统多功能性受植物物种多样性的制约。本研究可为青藏高原草地退化及恢复的过程和机理研究提供理论依据。  相似文献