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排序方式: 共有68条查询结果,搜索用时 15 毫秒
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Estepa JC Garfia B Gao PR Cantor T Rodriguez M Aguilera-Tejero E 《Equine veterinary journal》2003,35(3):291-295
REASONS FOR PERFORMING STUDY: Parathyroid hormone (PTH) plays a critical role in the regulation of mineral metabolism in mammals. Until recently, the standard method for PTH measurement has been the 2nd generation intact-PTH (I-PTH) assay. Current evidence indicates that the I-PTH assay binds to the PTH molecule and to an inactive N-terminally truncated PTH fragment that tends to accumulate in the blood of uraemic patients. Therefore, a new 3rd generation PTH assay that detects only the whole PTH molecule (W-PTH; cyclase-activating PTH [CAP]) has been developed. OBJECTIVES: To validate this more specific W-PTH assay for measurement of equine PTH and evaluate its clinical utility. METHODS: W-PTH and I-PTH were measured in plasma samples from normal horses (adults and foals) and horses with nutritional secondary hyperparathyroidism (N2HPT) and with chronic renal failure (CRF). Replicate measurements and dilutional paralellism were used for assay validation. Changes in blood ionized calcium were induced by EDTA and CaCl2 administration. RESULTS: Performance of the W-PTH assay (accuracy, sensitivity, specificity and ability to detect changes in PTH in response to changes in calcium) was similar to that of the I-PTH assay. Surprisingly, the relative W-PTH concentration in normal horses and foals was higher than the relative I-PTH concentration. W-PTH values remained higher than I-PTH during acute hypo- and hypercalcaemia. An increase in both W-PTH and I-PTH concentrations was found in horses with N2HPT. In horses with CRF, W-PTH and I-PTH values were very low and no increase in I-PTH was observed. CONCLUSIONS: The W-PTH assay can be used for measurement of equine PTH. POTENTIAL RELEVANCE: The use of W-PTH assay is likely to improve the diagnosis of mineral metabolism in horses. 相似文献
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Desneux Nicolas Han Peng Mansour Ramzi Arn Judit Brvault Thierry Campos Mateus R. Chailleux Anais Guedes Raul N. C. Karimi Javad Konan Kouassi Arthur J. Lavoir Anne-violette Luna Mara G. Perez-Hedo Meritxell Urbaneja Alberto Verheggen Franois J. Zappal Lucia Abbes Khaled Ali Abid Bayram Yunus Cantor Fernando Cuthbertson Andrew G. S. De Vis Raf Erler Fedai Firake Dnyaneshwar M. Haddi Khalid Hajjar M. Jamal Ismoilov Khasan Jaworski Coline C. Kenis Marc Liu Hao-tian Madadi Hossein Martin Thibaud Mazih Ahmed J. Messelink Gerben Mohamed Samira A. Nofemela Robert S. Oke Abiola Ramos Csar Ricupero Michele Roditakis Emmanouil Shashank Pathour R. Wan Fang-Hao Wang Ming-hui Wang Su Zhang Yi-Bo Biondi Antonio 《Journal of pest science》2022,95(1):17-39
Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato... 相似文献
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Ana Eguiguren Enrico Pirotta Kristina Boerder Maurício Cantor Godfrey Merlen Hal Whitehead 《水产资源保护:海洋与淡水生态系统》2021,31(6):1466-1481
- Sperm whales have occupied the waters off the Galápagos Islands, Ecuador, for at least the past 200 years. During the 19th century, they were the target of intensive whaling that severely depleted the population. In recent times, after commercial whaling ended, sperm whales in the region remain vulnerable to multiple threats, especially potential entanglement in fishing gear, which may hinder their ability to recover from the whaling era.
- As a highly mobile, long-lived species, long-term analysis of the habitat use of sperm whales is necessary to establish effective conservation and management strategies. Here, contemporary (1985–2014) and historical (1830–1850) sperm whale habitat use off the Galápagos Islands was analysed and contrasted to the extent of the Galápagos Marine Reserve (GMR). Contemporary habitat use and its variability over time were modelled as a function of geographic, oceanographic, and topographic variables using generalized additive models.
- The fine-scale habitat (<50 km) used by sperm whales was associated with topographic (i.e. depth and slope) and oceanographic characteristics (i.e. relative sea surface temperature and standard deviation of sea surface temperature), but these preferences varied over time.
- While historical and contemporary data indicate that sperm whale habitat primarily occurred within the boundaries of the GMR, in recent years, whales were found up to 30.1% of the time outside the GMR, potentially overlapping with commercial fisheries operating in the area.
- The dynamic nature of the relationship of this nomadic species with its habitat highlights the need of large-scale conservation efforts across the Eastern Tropical Pacific region, including the wide-scale enforcement of regulations requiring the use of Automatic Identification System in fishing vessels, the promotion of on-board fisheries observer programmes, the development of adaptive management strategies, and international collaboration to identify and mitigate threats.
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Two hundred feral cats from the inner suburbs of Sydney were examined post mortem for adult Dirofilaria immitis and circulating microfilariae, and 101 of these cats were tested for heartworm antigens by an ELISA. Only 2 cats (1%) had adult heartworms, the blood sample from another cat contained a single microfilaria. The blood of a further three cats contained small amounts of D immitis antigen. Although D immitis occurs in cats in Sydney, the prevalence is not high enough to warrant prophylactic treatment. 相似文献
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Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis 总被引:15,自引:0,他引:15
S H Giannini M Schittini J S Keithly P W Warburton C R Cantor L H Van der Ploeg 《Science (New York, N.Y.)》1986,232(4751):762-765
Chromosomes of four species of Leishmania represented by ten different geographic isolates were analyzed by pulsed field gradient gel electrophoresis (PFG) to assess chromosome stability in these parasitic protozoans. Among different geographic isolates of the same subspecies, more than two-thirds of chromosomes had similar sizes, ethidium bromide staining intensities, and locations of alpha,beta-tubulin genes. However, among New World Leishmania, members of different species or subspecies have fewer than one-third of their chromosomes in common. Therefore, PFG karyotypes of Leishmania exhibit intraspecific variability similar to that reported for other parasitic protozoans. The greater similarities of the karyotypes of members of the same Leishmania subspecies may indicate that they represent valid taxa. These similarities also allowed the use of PFG in clinical diagnosis for rapid and accurate typing of patient isolates. 相似文献
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