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Kernif T Socolovschi C Wells K Lakim MB Inthalad S Slesak G Boudebouch N Beaucournu JC Newton PN Raoult D Parola P 《Comparative immunology, microbiology and infectious diseases》2012,35(1):51-57
Rickettsioses and bartonelloses are arthropod-borne diseases of mammals with widespread geographical distributions. Yet their occurrence in specific regions, their association with different vectors and hosts and the infection rate of arthropod-vectors with these agents remain poorly studied in South-east Asia. We conducted entomological field surveys in the Lao PDR (Laos) and Borneo, Malaysia by surveying fleas, ticks, and lice from domestic dogs and collected additional samples from domestic cows and pigs in Laos. Rickettsia felis was detected by real-time PCR with similar overall flea infection rate in Laos (76.6%, 69/90) and Borneo (74.4%, 268/360). Both of the encountered flea vectors Ctenocephalides orientis and Ctenocephalides felis felis were infected with R. felis. The degrees of similarity of partial gltA and ompA genes with recognized species indicate the rickettsia detected in two Boophilus spp. ticks collected from a cow in Laos may be a new species. Isolation and further characterization will be necessary to specify it as a new species. Bartonella clarridgeiae was detected in 3/90 (3.3%) and 2/360 (0.6%) of examined fleas from Laos and Borneo, respectively. Two fleas collected in Laos and one flea collected in Borneo were co-infected with both R. felis and B. clarridgeiae. Further investigations are needed in order to isolate these agents and to determine their epidemiology and aetiological role in unknown fever in patients from these areas. 相似文献
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试验旨在研究柠檬酸和辣木茎叶粉的使用对产蛋后期蛋鸡的生产性能、蛋品质及血清抗氧化指标的影响。试验选取720只52周龄生产性能及体重相近的广西麻鸡,随机分为4组,每组180只。试验采用单因素设计,其中柠檬酸以添加剂的形式添加,辣木茎叶粉为等量替代基础日粮的方式加入,添加量分别为0.07%柠檬酸、0.07%柠檬酸+4%辣木茎叶粉、4%辣木茎叶粉,分别记为A组、B组、C组,对照组饲喂基础日粮。结果表明:与对照组相比,B组在产蛋率、蛋白高度、哈夫单位、蛋壳厚度、蛋壳强度均有一定程度的改善,但差异不显著(P>0.05)。与对照组相比,B组蛋黄粗脂肪含量显著提高(P<0.05),A组蛋黄粗脂肪含量显著降低,C组平均蛋重显著降低。与对照组相比,B、C组总超氧化物歧化酶、过氧化氢酶显著提高(P<0.05)。研究表明,柠檬酸和辣木茎叶粉的联合使用可以改善产蛋后期蛋鸡的生产性能、蛋品质和血清抗氧化能力。 相似文献
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试验以植酸酶(A)和枯草芽孢杆菌(B)、黑曲霉(D)、酿酒酵母菌(E)为发酵菌种进行固态厌氧发酵。在相同条件下比较不同菌的菌酶协同发酵辣木茎秆粉对发酵品质、营养成分以及抗营养因子植酸的影响。结果显示:A+D+E组、A+B+E组、A+B+D+E组对提高乳酸、降低pH值和氨态氮含量均有显著影响(P<0.05)。A+B+E组与A+B+D+E组显著提高组蛋白(CP)含量(P<0.05),A+D+E组与A+B+D+E组能显著降低中性洗涤纤维(NDF)与酸性洗涤纤维(ADF)含量(P<0.05)。A+B+D组、A+D+E组与A+B+D+E组显著降低植酸含量(P<0.05)。试验表明,混菌加酶发酵会比单菌加酶发酵具有更优的效果。选用枯草芽孢杆菌、黑曲霉、酿酒酵母与植酸酶混合发酵改善辣木茎秆粉的营养品质,降低抗营养因子植酸的效果最佳。 相似文献
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