关中驴对6株动物病毒抗原的免疫应答试验     

李六金  朱德生  贾满民  陈慧茹  吴耀南  赵永康  押宗保 《动物医学进展》2003,24(3):104-108

以关中驴为试验动物 ,用猪瘟病毒(HCV )、犬细小病毒 (CPV )、兔瘟病毒(RHDV)、鸡新城疫病毒 (NDV)、伪狂犬病毒(PRV)等病毒进行免疫 ,检测所产生的抗体效价 ,并与猪、鸡、兔、犬、及猫等动物进行比较。试验结果表明 ,关中驴与对照动物产生的抗体效价一致 ,证明了关中驴可替代其它动物用于生物制品研究  相似文献   

Frequency of Undetected Glove Perforation and Associated Risk Factors in Equine Surgery          下载免费PDF全文

Yvonne A. Elce  Sheila Laverty  Eduardo Almeida da Silveira  Perrine Piat  Pierre Trencart  Pavlina Ruzickova  Richard J. M. Reardon 《Veterinary surgery : VS》2016,45(8):1066-1070

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Expression of cyclooxygenase-1 and -2 in naturally occurring squamous cell carcinomas in horses     

Elce YA  Orsini JA  Blikslager AT 《American journal of veterinary research》2007,68(1):76-80

OBJECTIVE: To assess expression of cyclooxygenase (COX)-1 and -2 in naturally occurring squamous cell carcinomas (SCCs) and the analogous normal tissues in horses. SAMPLE POPULATION: Tissue samples collected from 3 conjunctival, 2 vulvar, 4 preputial, and 5 penile SCCs during surgical excision in 14 horses and from corresponding body regions (conjunctiva [n = 5 horses], vulva [2], prepuce [3], and penis [3]) in 5 horses euthanized for reasons unrelated to neoplasia. PROCEDURES: Tissue samples were snap frozen in liquid nitrogen and stored at -80 degrees C until analysis. Protein was extracted from the frozen tissues, and western blot analyses were performed. Nonneoplastic and abnormal tissues from each body region were run on the same blot, and blots were run in triplicate. Molecular-weight markers and COX-1 and 2 ovine standards (positive control samples) were run concurrently on the gels; negative control samples were not used. RESULTS: All tissues, including the nonneoplastic and SCC tissues, expressed both COX-1 and -2 proteins. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that the expression of COX proteins in both nonneoplastic and SCC-affected tissues in horses is markedly different from that in other species. The reason for the potential benefit of COX-2 inhibitors in horses and other species is unknown. Further research needs to be performed to evaluate the efficacy of COX-2 inhibitors as cancer treatments in horses. Investigation of the mechanisms of tumor development in horses should be performed to increase understanding of this disease and ascertain how the mechanisms differ from those in other animals.  相似文献   

Arthroscopic removal of dorsoproximal chip fractures of the proximal phalanx in standing horses     

Elce YA  Richardson DW 《Veterinary surgery : VS》2002,31(3):195-200

OBJECTIVE: To report a technique for, and outcome after, arthroscopic removal of dorsoproximal chip fractures of a proximal phalanx in standing horses. STUDY DESIGN: Retrospective study. ANIMALS OR SAMPLE POPULATION: A total of 104 horses, 1 to 13 years of age, with a dorsoproximal chip fracture of a proximal phalanx. METHODS: Horses were restrained in standing stocks and sedated with intravenous xylazine, detomidine, or a combination of both drugs. Local analgesia was achieved with 2% mepivacaine administered intra-articularly and by subcutaneous infiltration in a crescent-shaped block dorsal to the fetlock. Sterile drapes were placed on the surgical field, and impervious drapes were used on the hoof and floor. Arthroscopic portals were created in the dorsal pouch of the metacarpophalangeal or metatarsophalangeal joints to remove chip fractures. Statistical analysis was performed on race records for all Thoroughbred racehorses and compared with previously published studies. Operative and hospitalization times were compared with those of the general hospital population, and risks associated with general anesthesia were examined. RESULTS: No major operative or postoperative complications occurred. Ninety-one percent of racehorses raced after surgery with 78% returning to race at the same or higher level. CONCLUSION: Standing arthroscopic surgery can be performed successfully to remove dorsoproximal chip fractures of the proximal phalanx. CLINICAL RELEVANCE: Standing arthroscopic surgery is a valid alternative treatment for experienced surgeons to avoid the expense and potential risks associated with general anesthesia.  相似文献   

内蒙古自治区的沙漠及沙漠化土地   总被引：4，自引：0，他引：4  

董建林  雅洁 《内蒙古林业调查设计》2004,27(1):3-7

文章叙述了采取系统抽样的方法,通过现地调查和遥感解译相结合,获取样地信息,利用数理统计的原理计算出各类沙漠化土地面积,利用卫星遥感技术、卫星定位技术和地理信息系统相结合,确定内蒙古沙漠和沙漠化土地的分布。监测表明,截至1999年内蒙古沙漠和沙漠化土地面积为42.08×104km2,占内蒙古自治区总面积118.3×104km2的35.6%。与1994年全国沙漠化土地监测结果对比,5a来全区沙漠化土地面积增加了15629km2,年均增加3126km2。内蒙古沙漠和沙漠化土地面积居全国第二位,而沙漠化土地扩展速度和可治理沙漠化土地面积居全国第一位。  相似文献   

林业科技创新、推广与示范体系建设     

龙云英  郑亚东  丁思统 《江西林业科技》2001,(5):36-37

论述了林业科技创新、推广与示范体系建设在林业开发中的重要性，总结了上饶市的实践经验与体会。  相似文献   

黑曲霉中国株(3.758)葡萄糖氧化酶(GOD)基因的克隆与结构分析     

押辉远 《安徽农业科学》2008,36(2):454-456

[目的]为充分开发黑曲霉在轻工业中的用途。[方法]以黑曲霉中国株(3.758)基因组DNA为模板,用LATaqDNA聚合酶对葡萄糖氧化酶(GOD)基因进行PCR扩增,扩增片段纯化后与pUCm-T载体连接后,转化到大肠杆菌DH5α感受态细胞,经琼脂糖凝胶电泳法、酶切和PCR鉴定获得阳性克隆。对获得含GOD基因的克隆进行测序,分析其蛋白质氨基酸序列及限制性内切酶的图谱。[结果]经PCR扩增获得约1.8kb的片段。获得的GOD基因编码序列共1818bp,与报道的黑曲霉(ATCC9029)GOD基因序列仅有3个碱基之差。该基因序列具有多个限制性内切酶位点。黑曲霉不同菌株与中国株(3.758)GOD基因的同源性比较表明,不同黑曲霉菌株中GOD基因不同,而菌株ATCC9029与菌株NRRL-3是同一菌株。[结论]该研究获得了GOD基因的全长序列,为GOD的生物工程生产和相关植物基因工程奠定了基础。  相似文献   

彭阳县茹河流域水土保持及生态建设的研究   总被引：1，自引：0，他引：1  

常富礼  徐洁  雅玉霞  张有金  刘学军  杨永辉  刘建平 《黑龙江农业科学》2006,(3):63-66

茹河流域以提高生态效益、经济效益和社会经济持续发展为目标,以基本农田优化结构和高效利用及植被建设为重点,加大水土保持生态建设力度,调整不合理的土地利用结构和农业产业结构,改广种薄收为少种多收,提高土地生产率,努力发展自给性生产和商品生产,大力发展经济作物以增加群众的经济收入,提高农民生活水平;防护性治理与开发性治理相结合,综合开发、利用、保护水土资源和植物资源,逐步实现生态环境良性循环.  相似文献   

水分调控对冬小麦同化物分配与水分利用效率的影响研究   总被引：15，自引：0，他引：15  

任巍  姚克敏  于强  欧阳竹  王菱 《中国生态农业学报》2003,11(4):92-94

试验研究 12个灌溉组合方式对冬小麦干物质积累与分配、产量和水分利用效率的影响结果表明 ,地面灌水条件下不同处理的地上部干物质积累量由高至低依次为田间持水量 65%的处理 >50 %的处理 >80 %的处理 ;地下 3 0cm、50cm和 10 0cm灌水条件下田间持水量 80 %的处理 >65%的处理 >50 %的处理。产量以地下 3 0cm灌水× 80 %田间持水量处理最高 ,为 6512 .4kg/hm2 ,地下 50cm灌水× 50 %田间持水量处理产量最低 ,为3 568.1kg/hm2 。保持相同田间持水量的灌水处理 ,随灌溉深度的增加其作物水分利用效率呈增大趋势。地下3 0cm灌水× 80 %田间持水量处理和地下 3 0cm灌水× 65%田间持水量处理是较合理的节水灌溉组合方式  相似文献   

Geriatric sinus tumours – is there hope for the future?     

Y. A. Elce 《Equine Veterinary Education》2012,24(4):169-171

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