Sexual compatibility types of Bremia lactucae isolates originating from Lactuca serriola     

Aleš Lebeda  Ida Blok 《European journal of plant pathology / European Foundation for Plant Pathology》1990,96(1):51-54

Results are given on the occurrence of sexual compatibility types of seven isolates ofBremia lactucae originating fromLactuca serriola (prickly lettuce). It is concluded that the isolates studied are heterothallic. Both compatibility types (B1 en B2) were determined, but type B2 was prevalent. Sexual recombination ofB. lactucae isolates originating from wild and cultivated lettuce may occur.Samenvatting Zeven isolaten vanBremia lactucae, afkomstig vanLactuca serriola in Tsjechoslowakije, zijn onderzocht op hun sexuele compatibiliteitstype door ze te combineren met Nederlandse fysio's vanB. lactucae, afkomstig van cultuursla (L. sativa), waarvan het compatibiliteitstype (B1 of B2) bekend is. Alle isolaten vanL. serriola bleken heterothallisch te zijn, waarbij type B2 meer werd aangetroffen dan type B1. Sexuele recombinatie vanBremia-isolaten van wildeLactuca-soorten en cultuursla blijkt goed mogelijk te zijn.  相似文献   

Purification and characterization of feline ghrelin and its possible role     

Ida T  Miyazato M  Naganobu K  Nakahara K  Sato M  Lin XZ  Kaiya H  Doi K  Noda S  Kubo A  Murakami N  Kangawa K 《Domestic animal endocrinology》2007,32(2):93-105

Ghrelin, a novel 28-amino acid peptide with an n-octanoyl modification at Ser3, has been isolated from rat and human stomach as an endogenous ligand for the growth hormone secretagogue receptor. Here, we purified feline ghrelin and examined its possible physiological role in cats. The major active form of feline ghrelin is a 28-amino acid peptide octanoylated (C8:0) at Ser3; except for one amino acid residue replacement, this structure is identical to those of rat and human ghrelins. However, much structural divergence in peptide length and fatty acid modification was observed in feline ghrelin: peptides consisting of 27 or 26 amino acids lacking Gln14 and/or Arg28 were found, and the third serine residue was modified by octanoic acid (C8:0), decanoic acid (10:0), or unsaturated fatty acids (C8:1, C10:1 and C10:2). In agreement with the structural divergence, two kinds of cDNA with different lengths were isolated. Administration of synthetic rat ghrelin increased plasma growth hormone levels in cats, with a potency similar to that in rat or human. Plasma levels of ghrelin in cats increased approximately 2.5-fold after fasting. The present study indicates the existence of structural divergence in feline ghrelin and suggests that, as in other animals, ghrelin may play important roles in GH release and feeding in cats.  相似文献   

Induction of unseasonable hibernation and involvement of serotonin in entrance into and maintenance of its hibernation of chipmunks T. asiaticus     

Murakami N  Kono R  Nakahara K  Ida T  Kuroda H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2000,62(7):763-766

Chipmunks that had been housed at 22 degrees C under a light-dark cycle of 14L:10D for at least one year were exposed to a short photoperiod (10L:14D) and low temperature to induce unseasonable hibernation. We were able to induce hibernation at any time of year and there was no significant difference in the duration of the hibernation bout, the duration of interbout euthermia and duration of bouts of torpor throughout the year; however entrance into hibernation took about 60 days in summer but only about 30 days in any other seasons. In addition, interbout euthermia predominantly occurred during the light phase in winter, whereas in spring interbout euthermia occurred equally in the light and dark phases. These results suggest that both the circadian and circannual systems are linked to hibernation in chipmunks. Subcutaneous infusion of a serotonin antagonist, para-chlorophenylalanine (PCPA), facilitated entrance into and interrupted hibernation in aroused and hibernating chipmunks in summer, respectively. On the other hand, opioid antagonist, naloxone, did not affect hibernation, but extended the period of interbout euthermia. These results suggest that the role of serotonin in entrance into and maintenance of hibernation in chipmunks is independent of the circannual system, and that opioid system may not be involved in hibernation in chipmunks.  相似文献   

Effects of pressurization on structure, water distribution, and sensory attributes of cured ham: can pressurization reduce the crucial sodium content?   总被引：1，自引：0，他引：1  

Bertram HC  Wu Z  Straadt IK  Aagaard M  Aaslyng MD 《Journal of agricultural and food chemistry》2006,54(26):9912-9917

This study investigated the replacement of tumbling (intermittent vacuum tumbling for 6 h) with pressure treatment (7 MPa for 4 s) in the production of a cured ham product with the aim of elucidating if the pressure treatment could reduce the amount of salt added to obtain a satisfactory product. Confocal laser scanning microscopy (CLSM) revealed a pressure-induced loosening of the meat structure, and proton nuclear magnetic resonance (NMR) relaxometry revealed that this structural modification of the meat had an impact on water properties and water distribution in both cooked and cooked/fried products. Three salt levels (0.6, 1.1, and 1.7% w/w) were investigated, and sensory profiling revealed that the pressured-cooked meat obtained a significantly higher juiciness score at low and medium salt levels. In addition, sensory profiling assessments revealed that at the lowest salt concentration the pressured product was perceived to be saltier compared with the tumbled product; however, the difference was not significant and was absent upon frying. In contrast, in sensory time intensity analysis the maximum intensity and the total salt taste were significantly higher in the pressured samples at the low and medium salt levels in the cooked samples. In conclusion, the present study showed strong evidence that the structure and biophysical characteristics of cured ham is altered by pressurization and suggested that pressurization may reduce the critical amount of salt required in a ham product.  相似文献   

Effects of positive end‐expiratory pressure titration on gas exchange,respiratory mechanics and hemodynamics in anesthetized horses     

Aline M Ambrósio  Keila K Ida  Maria TMR Souto  Alexandre H Oshiro  Denise T Fantoni 《Veterinary anaesthesia and analgesia》2013,40(6):564-572

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Effects of brine salting with regard to raw material variation of Atlantic salmon (Salmo salar) muscle investigated by Fourier transform infrared microspectroscopy     

Böcker U  Kohler A  Aursand IG  Ofstad R 《Journal of agricultural and food chemistry》2008,56(13):5129-5137

Atlantic salmon fillets differing with regard to raw material characteristics (prerigor, postrigor, frozen/thawed) and salt content were investigated by FT-IR microspectroscopy and light microscopy. Local variation within each salmon fillet was further taken into account by sampling from the head and tail part separately as they vary in fat and moisture content. The highest salt uptake was achieved for frozen/thawed quality during brine-salting with 16% NaCl for 4 h, while the uptake was least for prerigor fish. At the same time, salting caused muscle fiber swelling of about 10% for both frozen/thawed and postrigor qualities. Differences in the FT-IR amide I spectral region were observed implying a change in the muscle protein secondary structure. Prerigor was least affected by brine-salting, having a final salt concentration of 2.2%, while postrigor had a NaCl content of 3.0% and frozen/thawed of 4.1%. Local variation within the fillets had an effect on the amide I absorption characteristics before as well as after salting. Salt uptake of the samples was affected by raw material quality and at the same time the degree of swelling of the myofibers was influenced by raw material character.  相似文献   

Phenotype of macular corneal dystrophy in Labrador Retrievers: A multicenter study     

Claudia Busse  Christiane Kafarnik  Rose Linn‐Pearl  Christelle Volmer  Kaspar Matiasek  Johana E. Premont  Thomas Dulaurent  Jean‐Yves Douet  Ida Gilbert  Sari Jalomäki  Katrin Trost  Pierre‐Francois Isard  Ryan Boyd  Isabelle Raymond 《Veterinary ophthalmology》2019,22(3):294-304

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Effects of 12 and 17 cmH2O positive end-expiratory pressure applied after alveolar recruitment maneuver on pulmonary gas exchange and compliance in isoflurane-anesthetized horses     

Felipe SRM. Andrade  Lara L. Facó  Keila K. Ida  Luis CLC. Silva  Denise T. Fantoni  Aline M. Ambrósio 《Veterinary anaesthesia and analgesia》2019,46(1):64-73

Objective

To compare static compliance (C_st) and alveolar–arterial oxygen tension difference [P(a–a)O₂] between positive end-expiratory pressures (PEEP) of 7, 12 and 17 cmH₂O applied after an alveolar recruitment maneuver (RM) in isoflurane-anesthetized horses.

Study design

Prospective, randomized, clinical study.

Animals

A group of 30 healthy adult horses undergoing arthroscopic surgery.

Methods

Animals in dorsal recumbency and mechanically ventilated with a tidal volume of 14 mL kg^?1 and 7 cmH₂O PEEP (control; n = 6) were subjected to an RM by increasing PEEP from 7 to 22 cmH₂O in 5 cmH₂O increments at 5 minute intervals, and then decreased similarly to PEEP of 17 (RM17; n = 8), 12 (RM12; n = 8) or 7 cmH₂O (RM7; n = 8). C_st and P(a–a)O₂ were assessed prior to (baseline) and after the RM at 5, 10, 15, 20, 40, 60 and 80 minutes after achieving each target PEEP, and during recovery from anesthesia.

Results

Post-RM improvements on P(a–a)O₂ were maintained (baseline versus 80 minutes) in RM12 [216 ± 77 mmHg (28.8 ± 10.3 kPa) versus 194 ± 39 mmHg (25.9 ± 5.2 kPa)] and RM17 [180 ± 86 mmHg (24.0 ± 11.6 kPa) versus 136 ± 75 mmHg [18.2 ± 10.0 kPa]). The improvements on C_st were maintained only in RM12 (0.80 ± 0.13 versus 0.98 ± 0.13 mL cmH₂O^?1 kg^?1). No such improvements were observed in RM7 and control. No significant differences were observed between groups during recovery from anesthesia.

Conclusions

and clinical relevance The 12 and 17 cmH₂O PEEP can be used to maintain the improvements on P(a–a)O₂ obtained after an RM. Only 12 cmH₂O PEEP maintained the post-RM increase on C_st. Such variables were not influenced by the 7 cmH₂O PEEP.  相似文献   

What is your diagnosis? Marked hyperchloremia in a dog     

Ida Piperisova  Jennifer A. Neel  Mark G. Papich 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(3):411-414

Abstract: A 5‐year‐old neutered male Cavalier King Charles Spaniel was evaluated for a 3‐week history of progressive paresis. The dog had been receiving potassium citrate capsules to acidify urine for the past 2 years because of an earlier history of urolithiasis. Results of neurologic examination, spinal cord radiography, and magnetic resonance imaging of the skull and spinal cord revealed no lesions that could have accounted for the neurologic signs. The main abnormalities on a clinical chemistry profile were marked hyperchloremia (179 mmol/L, reference interval 108–122 mmol/L) and an anion gap of ?50.4 mmol/L (reference interval 16.3–28.6 mmol/L). Because of the severe hyperchloremia, serum bromide concentration was measured (400 mg/dL; toxic concentration >150 mg/dL; some dogs may tolerate up to 300 mg/dL). Analysis of the potassium citrate capsules, which had been compounded at a local pharmacy, yielded a mean bromide concentration of 239 mg/capsule. Administration of the capsules was discontinued and there was rapid resolution of the dog's neurologic signs. This case of extreme bromide toxicity, which apparently resulted from inadvertent use of bromide instead of citrate at the pharmacy, illustrates the importance of knowing common interferents with analyte methodologies and of pursing logical additional diagnostic tests based on clinical and laboratory evidence, even when a patient's history appears to rule out a potential etiology.  相似文献   

Pulse pressure variation as a guide for volume expansion in dogs undergoing orthopedic surgery     

Denise T. Fantoni  Keila K. Ida  André M. Gimenes  Matheus M. Mantovani  Jacqueline R. Castro  Geni C.F. Patrício  Aline M. Ambrósio  Denise A. Otsuki 《Veterinary anaesthesia and analgesia》2017,44(4):710-718

Objective

To investigate whether pulse pressure variation (PPV) can predict fluid responsiveness in healthy dogs during clinical surgery.

Study design

Prospective clinical study.

Animals

Thirty-three isoflurane-anesthetized dogs with arterial hypotension during orthopedic surgery.

Methods

Fluid challenge with lactated Ringer's solution (15 mL kg^?1 in 15 minutes) was administered in mechanically ventilated dogs (tidal volume 10 mL kg^?1) with hypotension [mean arterial pressure (MAP) < 65 mmHg]. The volume expansion was considered effective if cardiac output (CO; transesophageal Doppler) increased by ≥ 15%. Cardiopulmonary data were analyzed using two-way ANOVA, receiver operating characteristics (ROC) curves and Spearman coefficient; p < 0.05 was considered significant.

Results

Effective volume expansion, mean ± standard deviation 42 ± 4% increase in CO (p < 0.0001) was observed in 76% of the dogs, resulting in a decrease in PPV (p < 0.0001) and increase in MAP (p < 0.0001), central venous pressure (CVP; p = 0.02) and ejection fraction (p < 0.0001) compared with before the fluid challenge. None of these changes occurred when volume expansion resulted in a nonsignificant CO increase of 4 ± 5%. No significant differences were observed in blood gas analysis between responsive and nonresponsive dogs. The increase in CO was correlated with the decrease in PPV (r = ?0.65; p < 0.0001) but absolute values of CO and PPV were not correlated. The PPV performance (ROC curve area: 0.89 ± 0.06, p = 0.0011) was better than that of CVP (ROC curve area: 0.54 ± 0.12) and MAP (ROC curve area: 0.59 ± 0.13) to predict fluid responsiveness. The best cut-off for PPV to distinguish responders and nonresponders was 15% (50% sensitivity and 96% specificity).

Conclusions and clinical relevance

In mechanically ventilated, healthy, isoflurane-anesthetized dogs, PPV predicted fluid responsiveness to volume expansion, and MAP and CVP did not show such applicability.  相似文献