An assessment of a Western blot method for the investigation of canine cutaneous adverse food reactions          下载免费PDF全文

Elisa Maina  Irina Matricoti  Chiara Noli 《Veterinary dermatology》2018,29(3):217-e78

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Q gene variability in wheat species with different spike morphology     

Irina Sormacheva  Kseniya Golovnina  Valeriya Vavilova  Kazumasa Kosuge  Nobuyoshi Watanabe  Alexandr Blinov  Nikolay P. Goncharov 《Genetic Resources and Crop Evolution》2015,62(6):837-852

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Detection of novel polymorphisms in the ckit gene of canine patients with lymphoma,melanoma, haemangiosarcoma,and osteosarcoma     

Irina Gramer  Martin Kessler  Joachim Geyer 《Veterinary research communications》2016,40(2):89-95

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Naturally transmitted herpesvirus papio-2 infection in a black and white colobus monkey     

Troan BV  Perelygina L  Patrusheva I  Wettere AJ  Hilliard JK  Loomis MR  Voe RS 《Journal of the American Veterinary Medical Association》2007,231(12):1878-1883

CASE DESCRIPTION: A 6.5-year-old female eastern black and white colobus monkey (Colobus guereza) was evaluated after acute onset of ataxia and inappetence. CLINICAL FINDINGS: The monkey was ataxic and lethargic, but no other abnormalities were detected via physical examination, radiography, or clinicopathologic analyses. During the next 2 days, the monkey's clinical condition deteriorated, and its WBC count decreased dramatically. Cytologic examination of a CSF sample revealed marked lymphohistiocytic inflammation. TREATMENT AND OUTCOME: Despite supportive care, the monkey became apneic; after 20 hours of mechanical ventilation, fatal cardiac arrest occurred. At necropsy, numerous petechiae were detected within the white matter tracts of the brain; microscopic lesions of multifocal necrosis and hemorrhage with intranuclear inclusions identified in the brain and adrenal glands were consistent with an acute herpesvirus infection. A specific diagnosis of herpesvirus papio-2 (HVP-2) infection was made on the basis of results of serologic testing; PCR assay of tissue specimens; live virus isolation from the lungs; and immunohistochemical identification of the virus within brain, spinal cord, and adrenal gland lesions. Via phylogenetic tree analysis, the colobus HVP-2 isolate was grouped with neuroinvasive strains of the virus. The virus was most likely transmitted to the colobus monkey through toys shared with a nearby colony of baboons (the natural host of HVP-2). CLINICAL RELEVANCE: To the authors' knowledge, this is the first reported case of natural transmission of HVP-2 to a nonhost species. Infection with HVP-2 should be a differential diagnosis for acute encephalopathy in primate monkeys and humans, particularly following exposure to baboons.  相似文献   

The effect of an energy additive on the metabolism of cattle     

Elina Nafikova  Irina Mironova  Igor Gazeev  Dmitry Blagov  Azat Nigmatiyanov 《Canadian journal of veterinary research》2021,85(3):210

The objective of this study was to determine the effect of an energy additive on the metabolism of cattle. This article provides information on the analysis of the diet of young cattle calculated for when the animals were both indoors and outdoors. The ration was prepared for 40 heifers, divided into 4 groups consisting of 10 animals in each group. Three of these groups were fed different amounts of a high-energy additive, which was not fed to the control group. The effectiveness of the additive was analyzed according to the balance experiment and by calculating digestibility coefficients. It was determined that the percentage of nitrogen use in young animals was higher in the groups that were fed the additive than in the control group. Increasing the dose of the additive increased the level of nitrogen use. Comparative analysis of live weight indicated intergroup differences in favor of heifers in the groups that were fed the additive of 1.34% to 2.41% at the age of 9 mo; 2.51% to 4.16% at 12 mo; 3.14% to 5.46% at 15 mo; and 3.57% to 6.30% at 18 mo. The average daily growth dynamics indicated a gradual increase in all animals up to 15 mo, with a slight decrease by 18 mo of age. The difference among the groups ranged from 5.08% to 8.85% at 6 to 9 mo of age; 7.08% to 10.79% at 9 to 12 mo; 5.64% to 10.97% at 12 to 15 mo; and 6.05% to 11.11% at 18 mo. It was concluded that feeding the energy additive Tanrem to heifers increased their metabolism so that nitrogen use was improved, and feed was digested more efficiently, which in turn improved the growth of animals. Using an energy additive at the mid-range dose of 500 g a day per animal is recommended, since the effect was similar at the mid-range and maximum dosages.  相似文献   

Effects of different five-day progesterone-based synchronization protocols on the estrous response and follicular/luteal dynamics in dairy cows     

Fernando LóPEZ-GATIUS  Irene LóPEZ-HELGUERA  Fabio DE RENSIS  Irina GARCIA-ISPIERTO 《The Journal of reproduction and development》2015,61(5):465-471

This study compared the responses shown by lactating dairy cows to four different P4-based protocols for AI at estrus. Cows with no estrous signs 96 h after progesterone intravaginal device (PRID) removal were subjected to fixed-time AI (FTAI), and their data were also included in the study. In Experiment I, follicular/luteal and endometrial dynamics were assessed every 12 h from the beginning of treatment until AI. The estrous response was examined in Experiment II, and fertility was assessed in both experiments. The protocols consisted of a PRID fitted for five days, along with the administration of different combinations of gonadotropin releasing hormone (GnRH), equine chorionic gonadotropin and a single or double dose (24 h apart) of prostaglandin F_2α. In Experiment I (40 cows), animals receiving GnRH at the start of treatment showed a significantly higher ovulation rate during the PRID insertion period while estrus was delayed. In Experiment II (351 cows), according to the odds ratios, cows showing luteal activity at the time of treatment were less likely to show estrus than cows with no signs of luteal activity. Treatment affected the estrous response and the interval from PRID removal to estrus but did not affect conception rates 28–34 days post AI. Primiparous cows displayed a better estrous response than multiparous cows. Our findings reveal acceptable results of 5-day P4-based protocols for AI at estrus in high-producing dairy cows. Time from treatment to estrus emerged as a good guide for FTAI after a 5-day P4-based synchronization protocol.  相似文献   

Hydrolytic Enzyme Inhibitors and Necrotic Reactions in Potato Leaves Reduce Reproductive Success of Colorado Potato Beetle     

Rinat Ismagilovich Ibragimov  ;Ildar Salimyanovich Mardanshin  ;Galina Vasilyevna Benkovskaya  ;Maksim Borisovich Udalov  ;Irina Andreevna Shpirnaya  ;Vyacheslav Olegovich Tsvetkov 《农业科学与技术》2014,(4):331-341

In field and laboratory conditions the reproductive potential （survival at different stages of development） of the Colorado potato beetle has been measured, the dependence of the parameters of insects fertility on varietal features of the feed has been investigated, the food activity of the representatives of insects from four areas of the Southern Urals has been measured, the intensity and time of hypersensitivity reactions on potato leaves has been defined. In laboratory experiments, the level of activity of hydrolytic enzymes in insects and the level of activity of these enzymes inhibitors in the leaves of three potato varieties differing in their resistance to the Colorado potato beetle have been determined. The results show that the insects-representatives of different samples have different food activity in relation to different potato varieties. High mortality rates were revealed at feeding with leaves of potato of the Bashkir variety characterized by a high level of hydrolases inhibitors and relatively rapid development of necrotic reactions after the appearance of eggs laying on the leaves. We suppose that the neerogenetic barrier appearing in the form of necrosis is the major cause of the fetal mortality of insects. The level of the content of inhibitors inactivating larvae digestive enzymes affects the survival of insects at postembryonic stages. The speed of the necrosis emergence, the level of activity of digestive enzymes inhibitors in potato can be considered as criteria for the selection of breeding material for resistance to the Colorado potato beetle.  相似文献   

Advances in the application of neuropeptides in insect control     

Miriam Altstein  Orna Ben-Aziz  Irit Schefler  Irina Zeltser  Chaim Gilon 《Crop Protection》2000,19(8-10):547-555

The development of a new approach for the generation of a novel type of putative insect control agents based on backbone cyclic peptidomimetic antagonists of insect-neuropeptides is reported. The approach, termed the backbone cyclic neuropetide based on autogonist (BBC-NBA) was applied to the insect pyrokinin/pheromone biosynthesis activating neuropeptide (PBAN) family as a model, and led to the discovery of a potent linear lead antagonist and several highly potent, metabolically stable BBC peptidomimetic antagonists, devoid of agonistic activity, which inhibited in vivo PBAN-mediated activities in moths.  相似文献   

ESLab application to a boreal watershed in southern Finland: preparing for a virtual research environment of ecosystem services     

Maria Holmberg  Anu Akujärvi  Saku Anttila  Lauri Arvola  Irina Bergström  Kristin Böttcher  Xiaoming Feng  Martin Forsius  Inese Huttunen  Markus Huttunen  Yki Laine  Heikki Lehtonen  Jari Liski  Laura Mononen  Katri Rankinen  Anna Repo  Vanamo Piirainen  Pekka Vanhala  Petteri Vihervaara 《Landscape Ecology》2015,30(3):561-577

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Comparative analysis of tobacco-specific nitrosamines and total N-nitroso compounds in moldovan cigarette tobacco     

Stepanov I  Hecht SS  Mirvish SS  Gonta M 《Journal of agricultural and food chemistry》2005,53(20):8082-8086

While previous studies have evaluated levels of tobacco-specific nitrosamines (TSNA) and total N-nitroso compounds (NOC) in tobacco, there are no reports in the literature on TSNA and total NOC in the same tobacco products. We compared levels of TSNA, total NOC, and NOC precursors (NOCP) in tobacco of cigarettes purchased in Moldova and in some tobacco types commonly used for the manufacturing of Moldovan cigarettes. Cigarette tobaccos included those from non-Moldovan, traditional Moldovan, and blended Moldovan cigarettes. The results demonstrate that tobacco of non-Moldovan cigarettes contains higher TSNA and NOC levels (mean, 16 and 63 nmol/g tobacco, n = 6) than that of Moldovan cigarettes (mean, 5 and 23 nmol/g tobacco, n = 25). TSNA and NOC levels were also generally higher in tobacco of blended than in traditional Moldovan cigarettes. NOCP levels in Moldovan and non-Moldovan cigarette tobacco were similar as follows: 29000 +/- 30000 and 33000 +/- 28000 nmol/g tobacco (mean +/- SD). Total NOC were strongly correlated with total TSNA levels (r = 0.66; P < 0.0001). These findings demonstrate that current technologies involved in the manufacture of some blended cigarettes create conditions that favor N-nitrosation of alkaloids and other tobacco constituents.  相似文献