排序方式： 共有2条查询结果，搜索用时 0 毫秒
1
1.
Anna Trojak-Goluch Dorota Laskowska Monika Agacka Diana Czarnecka Magdalena Kawka Anna Czubacka 《Breeding Science》2011,61(4):389-393
Black root rot (BRR) caused by Thielaviopsis basicola as well as Tomato spotted wilt virus (TSWV) are the most serious problems in tobacco growing regions. We crossed the breeding line WGL 3 carrying BRR resistance derived from N.glauca with the line PW-834 the resistance of which to TSWV was transferred from cultivar Polalta. Anthers obtained from F1 hybrid plants were cultured to induce haploids combining resistance to Th. basicola and TSWV. Flow cytometry analysis revealed 242 haploids and 2 spontaneous doubled haploids among regenerants. All haploids were cloned and then evaluated for BRR as well as TSWV resistance. The presence of pathogens was detected by microscopic evaluation of roots or using DAS-ELISA test. Microscopic assessment showed that, 132 haploids had no symptoms of Th. basicola which, together with the absence of symptoms in the F1 hybrids, indicated a dominant monogenic mode of inheritance. At the same time only 30 haploids demonstrated resistance to TSWV. SCAR markers associated with TSWV resistance gene detection was applied. The results indicate that small proportion of TSWV-resistant haploids is probably due to the influence of deleterious genes flanking the resistance factor that reduced vitality of gametophytes. Altogether, 24 haploids showed multiple resistance to Th. basicola and TSWV. 相似文献
2.
Nikravesh-Masouleh Tahereh Seidavi Alireza Kawka Magdalena Dadashbeiki Mohammad 《Tropical animal health and production》2018,50(3):635-641
Tropical Animal Health and Production - The aim of this study was to evaluate the effect of different dietary energy and protein supplements on performance, weekly body sizes or body frame size,... 相似文献
1