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Bozkurt İ. Adem Soylu Soner Kara Merve Doksöz Senem Filiz Altan Bülent Çarpar Hakan 《植物病害和植物保护杂志》2023,130(1):13-21
Journal of Plant Diseases and Protection - Citrus (Citrus spp. L.), which has high nutritional and economic value, is one of the most commonly grown fruits in Turkey. In spring 2020, large aerial... 相似文献
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Yusuf Bozkurt Fatih Öğretmen Uğur Erçin Ümmügülsüm Yιldιz 《Aquaculture Research》2008,39(15):1666-1672
In this research, the mineral and organic composition of the seminal plasma, physical spermatological parameters and their physiological relationships were investigated in grass carp (Ctenopharyngodon idella). The seminal plasma contained 98.14±5.23 mM L?1 (Na+), 380.85±25.95 mM L?1 (K+), 30.25±4.96 mg dL?1 (Ca2+), 19.16±1.70 mEq L?1 (Mg2+), 1.36±0.11 mg dL?1 glucose, 0.37±0.08 g dL?1 total protein, 12.02±1.18 mg dL?1 cholesterol, 14.85±1.50 mg dL?1 triglyceride and 43.5±9.56 mg dL?1 urea. The following spermatological parameters were found: sperm volume 14.44±1.16 mL, sperm motility 80.60±1.55%, movement duration 67.68±4.32 s, density 15.43±0.72 × 109 mL?1, total density 337.43+45.86 × 109 and pH 7.24±0.17. The Na+ and Ca2+ ions correlated negatively with spermatozoa motility (r=?0.453, P>0.05 and r=?0.192, P>0.05) respectively. The K+ ion correlated positively with spermatozoa motility (r=0.545, P>0.05). But a statistically significant correlation was not observed between sperm motility and seminal plasma parameters. The following correlations were observed between mineral and organic components. The Mg2+ was positively correlated with glucose and cholesterol (r=0.692, P<0.05 and r=0.680, P<0.05) respectively. A highly significant positive relationship was also found between Mg2+ and total protein (r=0.837, P<0.01). On the other hand, a significantly negative relationship was found between Ca2+ and triglyceride (r=?0.639, P<0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of grass carp sperm. 相似文献
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This study augments knowledge of bone growth by observing the development of the hyoid bone in the New Zealand White rabbit.
Preserved hyoid bones representing five different age periods, each period including five individuals and the total number
of animals being 25, were fixed in 3.5% formaldehyde solution and 95% ethanol, followed by a pure acetone bath. They were
then stained with an alcian blue–alizarin red combination. The mode of bone formation was intracartilaginous type ossification.
While the basihyoideum and thyrohyoideum were observed to start ossifying first at prenatal stage, indicating that they are
the main skeletal structures of the hyoid apparatus, the ceratohyoideum and lingual process began to ossify in the second
and third periods. The separately occurring primary ossification centres fused completely among themselves in the fifth period.
Because no further ossification centers were observed and the ossified parts continued the development and growth, the research
was terminated after 10 weeks of age. 相似文献
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The aim of the present study was to determine the effect of various cryoprotectants on post-thaw sperm quality and fertilizing capacity of cryopreserved scaly carp (Cyprinus carpio) semen. The present study focused on freezing of scaly carp sperm utilizing a practical and inexpensive protocol for aquaculture. Semen was diluted with Kurokura’s extender composing 3.6 g/l NaCl, 10 g/l KCl, 0.22 g/l CaCl2, 0.08 g/l MgCl2 and 0.2 g/l NaHCO3. The extender contained three different cryoprotectants (DMSO, DMA and egg yolk) at ratios of 5, 10 and 15 %. Semen was placed into 0.25-ml straws and exposed to liquid nitrogen vapor (?120 °C) using an insulated box with an adjustable tray for 10 min and then plunged into liquid nitrogen (?196 °C) tank. The thawing process was performed in a water bath at 40 °C for 10 s. The results indicated that type of cryoprotectants and their concentrations are rather effective in scaly carp sperm cryopreservation on post-thaw sperm quality, while they are very important in order to obtain high fertilization rates. The highest fertilization rate was determined as 96.4 ± 0.15 % with 15 % egg yolk, while the highest hatching rate was determined as 99.3 ± 0.80 with 15 % DMA. In conclusion, the applied cryopreservation method for scaly carp sperm is suitable to fertilize high amounts of eggs. 相似文献
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Tanyildizi S Bozkurt T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(7):775-780
The effects of lincomycin-spectinomycin and sulfamethoxazole-trimethoprim combinations on the hyaluronidase enzyme of serum and semen and on sperm characteristics in rams were determined. Thirthy-two Akkaraman rams were used. The rams were randomly divided into four groups. Group A and group B were determined as control groups of group C (lincomycin-spectinomycin) and D (sulfamethoxazole-trimethoprim), respectively. Combinations of lincomycin-spectinomycin and sulfamethoxazole-trimethoprim were administered at doses of 15 mg.kg(-1) intramuscularly and 12 mg.kg(-1) body weights orally, respectively. Blood and semen samples were collected at 4, 12, 24, 48, 72, 192 and 384 hr. Semen hyaluronidase activities of rams in group C increased significantly (p<0.001, <0.05) compared with the control group at 24 and 48 hr, respectively. Semen hyaluronidase activities in group D rams also increased significantly (p<0.001) in comparison with the control group at all times except 72 and 384 hr. Serum hyaluronidase activities increased significantly (p<0.01, <0.001) at 24 and 48 hr after treatment of lincomycin-spectinomycin. Additionally, significant (p<0.05, <0.001) increases were detected in the serum hyaluronidase activities of group D at 48 and 72 hr, respectively. No significant correlation was found between serum and semen hyaluronidase activities. Furthermore, significant increases (p<0.05) were observed in the percentages of motile sperm in the rams of group C and D compared with the control groups. The values of sperm concentration and total number of sperm in group C and D rams decreased significantly (p<0.001) in comparison with control groups. No significant correlations were found between the semen hyaluronidase activities and sperm characteristics. In conclusion, these findings show that the combinations of lincomycin-spectinomycin and sulfamethoxazole-trimethoprim do not have any harmful effects on hyaluronidase activities and sperm motility. However, the use of both antibiotic combinations in breeding rams during the ramming season is not advisable due to the decrease of sperm concentration. 相似文献
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Erhan Ozenc Mehmet Fatih Bozkurt Ebubekir Yazici Esra Seker Alev Gurol Bayraktaroglu Umit Ozcinar Nurhan Dogan 《Reproduction in domestic animals》2020,55(5):559-566
This study aimed to examine the teat characteristics in relation to the animal temperament during milking in the Anatolian buffaloes using ultrasonographic, histomorphological and immunohistochemical methods. The teat canal length (TCL), teat wall thickness (TWT), teat cisternal diameter (TCD), teat diameter (TD), teat length (TL), and teat circumference (TC) values in docile (n = 5) and nervous (n = 7) buffaloes were measured at the 0th, 3rd and 6th minute of stimulation. In additional experiments, comparative histomorphology and immunohistochemical examinations of buffalo (n = 7) and cow teats (n = 8) were performed. It was determined that post-stimulation mean TCL values were significantly higher in nervous buffaloes than those of teats in docile buffaloes (p < .05). A significant positive correlation between TCD and TD, TL and TC in both docile and nervous buffaloes was noted (p < .05). Unlike nervous buffaloes where only 3/14 teat canals were open by 3rd minute of milking stimulation, almost all (9/10) teat canals were observed opened in docile buffaloes. There were fewer muscle but more collagen bundles in buffalo teats compared with cow teats. It seems that temperament of animal during milking effects the milking efficiency, and in nervous buffaloes, probably the stimulation alone may not be sufficient for opening of the teat canal and hence achieve complete milking. 相似文献
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This study used the New Zealand White rabbit to reveal the normal ossification development of the cervical component of the
spine. Preserved cervical vertebrae representing five different age periods, each period including five individuals and the
total number of animals being 25, were fixed in 3.5% formaldehyde solution and 95% ethanol, followed by a pure acetone bath.
The materials were then stained with an alcian blue–alizarin red combination. The ossification centres were identical over
time, and the pattern of fusion among them was homogenous and constant in appearance. There were three different primary ossification
centres in all the cervical vertebrae except the axis, which showed four primary ossification centres. The dorsally located
primary ossification centres later formed the pedicles of the neural arches, while the ventral centres constituted the body
of each vertebra. The study was terminated at 10 weeks of age because the ossification centres observed in the cervical vertebrae
completed their fusion and no further ossification centres were observed. 相似文献