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1.
Masafumi Katayama Tomokazu Fukuda Toshimitsu Hatabu Kiyoaki Narabara Asaki Abe Yasuhiro Kondo 《Animal Science Journal》2014,85(3):277-285
In chickens, although estrogen receptors (ER) are reported to be associated with the immunological processes, detailed information about the differences in ER expression in the tissues related to the development of lymphocytes is not fully known, especially during the developmental stage. To learn more about this immunological relationship, we used semi‐quantitative polymerase chain reaction method to detect the ER expression levels in the thymus tissues of chicks during the developmental stage. Furthermore, ER‐expressing cells were detected by immunohistochemistry. The results of this study show that the expression level of ER increased on embryonic day 16 and decreased on day 20. Furthermore, ER expression was significantly higher in male than in female chickens at day 16. The increased expression on day 16 and decreased level on day 20 were also reproduced in the incidence of immunoreactive cells, although there was a 1‐day delay in the elevated incidence of the cells. This study revealed the changes in ER expression and the incidence of ER‐positive cells in the thymus of chickens during the developmental stage. 相似文献
2.
Takumi Kayukawa Kenjiro Furuta Kiyoaki Yonesu Takayoshi Okabe 《Journal of Pesticide Science》2021,46(1):53
Juvenile hormone (JH) is an insect-specific hormone that regulates molting and metamorphosis. Hence, JH signaling inhibitors (JHSIs) and activators (JHSAs) can be used as effective insect growth regulators (IGRs) for pest management. In our previous study, we established a high-throughput screening (HTS) system for exploration of novel JHSIs and JHSAs using a Bombyx mori cell line (BmN_JF&AR cells) and succeeded in identifying novel JHSIs from a chemical library. Here, we searched for novel JHSAs using this system. The four-step HTS yielded 10 compounds as candidate JHSAs; some of these compounds showed novel basic structures, whereas the others were composed of a 4-phenoxyphenoxymethyl skeleton, the basic structure of several existing JH analogs (pyriproxyfen and fenoxycarb). Topical application of seven compounds to B. mori larvae significantly prolonged the larval period, suggesting that the identified JHSAs may be promising IGRs targeting the JH signaling pathway. 相似文献
3.
Cytosolic Ca2+ is known to be an important factor in intracellular signaling pathways that regulate several cellular functions. The present study was designed to measure the intracellular concentrations of Ca2+ ([Ca2+ ]i ) in T cell mitogen-stimulated chicken lymphocytes, and to compare the results with those in rat lymphocytes. [Ca2+ ]i was increased in the thymocytes, splenocytes and bursacytes of chickens, and in the thymocytes and splenocytes of rats following exposure to the mitogens phytohaemagglutinin (PHA) and concanavalin A (ConA). Increases were greatest in the thymocytes followed by the splenocytes and bursacytes. The PHA-induced changes in the thymocytes and splenocytes were similar in chickens and rats, but the ConA-induced increases were significantly lower in the chickens than rats. Pretreatment with EGTA before the application of PHA and ConA completely suppressed the rise in [Ca2+ ]i in all the chicken lymphocytes, indicating that the increases that occurred in PHA- and ConA-treated chicken lymphocytes could be entirely attributed to the influx of extracellular Ca2+ . On the other hand, the PHA- and ConA-induced increase in [Ca2+ ]i in rat lymphocytes was not completely suppressed by EGTA, indicating the recruitment of Ca2+ from the intracellular Ca2+ pool. The results suggest species differences in the Ca2+ -based responses to T cell mitogens between chicken lymphocytes and rat lymphocytes. 相似文献
4.
Masahiko Mori Kento Maki Tsubasa Kawahata Daigo Kawahara Yuta Kato Toru Yoshida Hidetaka Nagasawa Hitoshi Sato Atsushi J. Nagano Paul C. Bethke Kiyoaki Kato 《Breeding Science》2021,71(2):208
Epicotyl length (ECL) of adzuki bean (Vigna angularis) affects the efficiency of mechanized weeding and harvest. The present study investigated the genetic factors controlling ECL. An F2 population derived from a cross between the breeding line ‘Tokei1121’ (T1121, long epicotyls) and the cultivar ‘Erimo167’ (common epicotyls) was phenotyped for ECL and genotyped using simple sequence repeats (SSRs) and single-nucleotide polymorphism (SNP) markers. A molecular linkage map was generated and fifty-two segregating markers, including 27 SSRs and 25 SNPs, were located on seven linkage groups (LGs) at a LOD threshold value of 3.0. Four quantitative trait loci (QTLs) for ECL, with LOD scores of 4.0, 3.4, 4.8 and 6.4, were identified on LGs 2, 4, 7 and 10, respectively; together, these four QTLs accounted for 49.3% of the phenotypic variance. The segregation patterns observed in F5 residual heterozygous lines at qECL10 revealed that a single recessive gene derived from T1121 contributed to the longer ECL phenotype. Using five insertion and deletion markers, this gene was fine mapped to a ~255 kb region near the end of LG10. These findings will facilitate marker-assisted selection for breeding in the adzuki bean and contribute to an understanding of the mechanisms associated with epicotyl elongation. 相似文献
5.
Yohei Nakamura Takuro Shibuno Nobuaki Suzuki Jun Nakamori Kiyoaki Kanashiro Yoshiro Watanabe 《Fisheries Science》2010,76(3):503-510
Molecular and otolith analyses were conducted for 173 settlement-stage larvae of emperor fishes (family Lethrinidae) collected
by light traps at Ishigaki Island, southern Japan, in July and August (summer season), to (1) present diagnostic DNA markers
for identification of lethrinid species and (2) compare the size and age at settlement of each species. PCR–RFLP and direct
nucleotide sequencing analyses identified 8 species. Size (standard length, SL) at settlement differed significantly between
species; Lethrinus ornatus (mean SL ± SD, 12.8 ± 1.5 mm), L. obsoletus (14.2 ± 0.8 mm) and L. harak (15.8 ± 1.6 mm) settled at a smaller size than L. atkinsoni (17.0 ± 1.3 mm), L. genivittatus (17.3 ± 1.0 mm), L. olivaceus (18.1 ± 0.6 mm), L. nebulosus (18.6 ± 4.2 mm), and L. sp.2 reported by Lo Galbo et al. (J Mol Evol 54:754–762, 2002) (21.7 ± 1.4 mm). Age at settlement tends to increase with
settlement size; L. obsoletus (mean age ± SD, 25.6 ± 1.2 days), L. atkinsoni (26.1 ± 2.1 days) and L. ornatus (26.3 ± 2.9 days) were younger at settlement than L. nebulosus (28.4 ± 2.1 days), L. harak (29.2 ± 1.7 days), L. olivaceus (29.5 ± 1.0 days), L. genivittatus (30.5 ± 1.7 days) and L. sp.2 (31.0 ± 2.0 days). Although our study showed interspecific variation in body size and age at settlement among 8 lethrinid
species, further seasonal replication is necessary to clarify the general patterns. 相似文献
6.
The growth, sex ratio with age, and age at sexual maturation were determined based on sectioned otoliths in 257 specimens
of the blackspot tuskfish Choerodon schoenleinii collected in waters off Ryukyu Island. Opaque rings observed by reflected light in the sectioned otoliths were found to form
once a year from January to July. The three growth parameters of the von Bertalanffy growth equation were L∞ = 68.1 (cm), k = 0.263, and t
0 = −0.023 (year). The age at which the sex ratio reached 50% by sexual transition was about 6.15 years, and the age at which
50% of females were sexually mature was approximately 2 years. The oldest specimen among the samples was 17 years old. 相似文献
7.
Young-Ha SHIN Shunta SHIRAISHI Kiyoaki NARABARA Asaki ABE Yasuhiro KONDO 《Animal Science Journal》2012,83(2):156-161
Estrogen has been reported to act on B cell genesis in the bursa of Fabricius of chick embryos. In this study, we attempted to demonstrate the hypothesis that B cell genesis is controlled by estrogen receptor (ER) in the bursal cells and steroidogenic enzymes synthesized in the bursa. We previously reported the presence of estrogen receptor α (ERα) in the bursa during the late stage of embryogenesis and an increase in the expression of ERα messenger RNA (mRNA) between the 13th day and 16th day. The number of ER-positive cells was maximal on the 16th day. In the present study, ER-positive cells in the bursa during the late stage of embryogenesis increased 4 h after β-estradiol treatment on the 14th to 18th day. The concentration of β-estradiol in the embryonic bursa increased. These results suggest that this stage of embryogenesis is critical in B cell development in the bursa in connection with the effect of estrogen treatment. Our findings also showed that the mRNA expression of five steroidogenic enzymes occurred in the bursa of chick embryos. These results suggest that estrogen is synthesized in the embryonic bursa and estrogen acts on the bursal cells in a paracrine fashion. 相似文献
8.
Grain yield-related traits and grain quality-related traits are important for rice cultivars. The quantitative trait loci (QTLs) involved in controlling the natural variation in these traits among closely related cultivars are still unclear. The present study describes the development of a novel chromosome segment substitution line (CSSL) population derived from a cross between the temperate japonica cultivars Yukihikari and Kirara397, which are grown in Hokkaido, the northernmost limit for rice cultivation. Days to heading, culm length, panicle length, panicle number, brown grain weight per plant, thousand brown grain weight, brown grain length, brown grain width, brown grain thickness, apparent amylose content, and protein content were evaluated. Panicle length, brown grain length and amylose content differed significantly in the parental cultivars. Thirty-five significant changes in the evaluated traits were identified in the CSSLs. A total of 28 QTLs were located on chromosomes 1, 2, 3, 4, 5, 6, 8, 9, 10, 11 and 12. These findings could be useful for breeding rice cultivars in the northernmost limit for rice cultivation. 相似文献
9.
云南隆庆关秋季鸟类环志研究 总被引：10，自引：0，他引：10
１９９７年９月２６日至１０月７日,在云南巍山县隆庆关（２５°１８′Ｎ,１００°２１′Ｅ）及其周围地区共网捕到鸟类８７种２５００多只,环志８０种２４１０只。除白日网捕到１０种２２只外,其余皆为夜间网捕。年龄鉴定１１３８只,成鸟与幼鸟的比率约为１∶２,性别鉴定８３９只,雌鸟与雄鸟比率约为２∶１。加上１９９５和１９９６两年同期网捕结果,共网捕鸟类９４种,环志８７种２９４２只,依重要性值（ＩＶ）,最重要的鸟类是红喉姬（Ｆｉｃｅｄｕｌａｐａｒｖａ）、树鹨（Ａｎｔｈｕｓｈｏｄｇｓｏｎｉ）和红尾伯劳（Ｌａｎｉｕｓｃｒｉｓｔａｔｕｓ）。此外,还讨论了每日捕鸟数量与天气条件的关系。 相似文献
10.
Kiyoaki NARABARA Asaki ABE GERILECHAOGETU Hamza HANIEH Yasuhiro KONDO 《Animal Science Journal》2009,80(6):669-677
Flow cytometric analysis and immunohistochemical observation were used to qualitatively and quantitatively clarify the nature of B cell differentiation in the bursa of Fabricius of chick embryos and to determine the timing of antibody class switching in chicken spleens based on positivity of IgM and IgG on and in the cells. In the bursa, the sIgM‐positive cell population formed from the 12th to 15th day of embryogenesis. The proportion of sIgM‐high expressing (sIgMhigh) cells was lower among bursacytes than splenocytes of hatched chicks, suggesting that the sIgMhigh bursacytes are to be released to peripheral sites. The proportion of sIgMhigh cells was higher at 0 days old than at any other examined stage of development. Colonization of the spleen by B cells occurred between the 18th day of embryogenesis and 0 days old. Antibody class switching was thought to start in the spleen between 1 and 2 weeks of age, because IgG‐positive cells were present in the spleen of 2‐week‐old chicks, but not 0‐day‐old or 1‐week‐old chicks. 相似文献