图书馆学情报学核心期刊网络引文著者的计量分析   总被引：1，自引：1，他引：0  

龚军 《农业图书情报学刊》2006,18(11):137-140

以10种图书馆情报学核心期刊2002～2004年的载文情况为样本．对这3年来的网络引文的著者情况进行了调研、分析。包括著作所属单位、分布地区、以覆职祢、学历、年龄等，旨在更准确地研究图书情报界对网络学术信息的利用情况。  相似文献   

PCR方法鉴别肉骨粉中的动物成份种类   总被引：9，自引：0，他引：9  

李林徐江涛  张永强王志亮 《中国动物检疫》2004,21(4):29-31

为防范疯牛病传入我国,有必要鉴别肉骨粉中动物成份的种类。针对牛、羊、猪和鸡四种动物的特异性核苷酸序列,分别选用和设计了四对特异性引物,用试剂盒提取四种动物的肉骨粉或者处理过的肉组织中的DNA,然后进行PCR扩增,所扩增的目的片断大小分别为271bp、199bp、196bp、148bp,运用PCR技术建立了鉴定这四种动物源性成分的方法。结果分别扩增出四种动物的特异性条带,证明该PCR方法具有很高的特异性和敏感性,而且成本低廉,简便易行,因此可以作为鉴别肉骨粉种类的常规方法。  相似文献   

Fermentation of dietary fibre in the intestinal tract of rats — a comparison of flours with different extraction rates from six cereals     

Margareta Nyman  Nils-Georg Asp  Birthe Pedersen  Bjøorn O. Eggum 《Journal of Cereal Science》1985,3(3):207-219

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Metabolic fate of [¹⁴C]endrin in bluegill fish     

P. Sudershan  M.A.Q. Khan 《Pesticide biochemistry and physiology》1980,14(1):5-12

Bluegill absorbed 85% of 1 ppb of endrin from water within 48 hr under static exposure conditions. The absorbed radiocarbon was eliminated linearly with a half-life of about 4 weeks. Analyses of eliminated radioactivity revealed only conjugated metabolites. 12-anti-Hydroxyendrin and 12-syn-hydroxyendrin were tentatively identified by cochromatography using thin-layer chromatography/autoradiography and gas chromatography. These metabolites were also present as conjugates in the fish organs. Seventy-three percent of the absorbed radioactivity recovered from fish extracts was in the form of unchanged endrin.  相似文献   

The metabolism of R-20458 [(E)-6,7-epoxy-1-(4-ethylphenoxy)-3,7-dimethyl-2-octene] in rat hepatocyte suspensions     

Antonio Morello  Yolanda Repetto  Robert A. White  Moises Agosin 《Pesticide biochemistry and physiology》1980,14(1):72-80

The metabolism of R-20458 [(E)-6,7-epoxy-1-(4-ethylphenoxy)-3,7-dimethyl-2-octene] by rat hepatocytes has been analyzed and compared with that of juvenile hormone I [methyl-(E,E)-cis-10,11-epoxy-7-ethyl-3,11-dimethyl-2,6-tridecadienoate] under identical conditions. The metabolism of R-20458 is characterized by the predominance of NADPH-dependent cytochrome P-450 and epoxide hydrolase reactions; whereas, JH I is metabolized mainly by carboxylesterase, epoxide hydrolase, and glutathione S-transferases. The metabolites of R-20458 have been shown to correspond to (E)-6,7-epoxy-1-(4-hydroxyethylphenoxy)-3,7-dimethyl-2-octene; (E)-6,7-epoxy-1-(4-acetylphenoxy)-3,7-dimethyl-2-octene; (E)-6,7-dihydroxy-1-(4-ethylphenoxy)-3,7-dimethyl-2-octene; and, (E)-6,7-dihydroxy-1-(4-acetylphenoxy)-3,7-dimethyl-2-octene. The production of the α-hydroxyethyl, p-acetylphenoxy, and acetylphenoxy-6,7-diol metabolites is markedly inhibited by SKF 525-A. No dramatic effects are produced by diethylmaleate and 1,2-epoxy-3,3,3-trichloropropane.  相似文献   

Comparative inhibition of the juvenile hormone esterases from Trichoplusia ni,Tenebrio molitor, and Musca domestica     

Thomas C. Sparks  Bruce D. Hammock 《Pesticide biochemistry and physiology》1980,14(3):290-302

Twenty-seven compounds were screened as potential inhibitors of juvenile hormone esterases. Of these compounds O-ethyl-S-phenyl phosphoramidothiolate provided the best inhibition for the cabbage looper, Trichoplusia ni (Hubner), and the yellow mealworm, Tenebrio molitor L., while the juvenile hormone esterases of the house fly, Musca domestica L., were best inhibited by a juvenoid carbamate (1-(m-phenoxy-N-ethyl carbamate)-3,7-dimethyl-7-methoxy-2E-octene). The inhibition patterns of T. ni and T. molitor are similar, while those of M. domestica are relatively different. Further studies on the juvenile hormone and α-napthyl acetate esterases of T. ni showed that they could be differentially inhibited. Diisopropyl phosphorofluoridate and an alkyl trifluoromethyl ketone selectively inhibit the hydrolysis of α-naphthyl acetate and juvenile hormone, respectively, while O-ethyl-S-phenyl phosporamidothiolate inhibits both enzymes. The juvenile hormone esterases of T. ni also appear to be unique enzymes that are selective for juvenile-hormone-like molecules. The in vivo inhibition of T. ni juvenile hormone esterases by O-ethyl-S-phenyl phosphoramidothiolate slows the in vivo hydrolysis of juvenile hormone and results in delayed pupation and malformed larvae that resemble larval-pupal intermediates. Thus, the esterases involved in juvenile hormone metabolism appear to be important in juvenile hormone regulation.  相似文献   

Effects of chlordimeform on mitochondria from eggs of Spodoptera littoralis     

Clemens Kötter 《Pesticide biochemistry and physiology》1978,9(3):263-267

Mitochondria were isolated from eggs of Spodoptera littoralis. With succinate (+pyruvate) as substrate, respiratory control ratios between 1.70 and 2.54 were obtained. Uncouplers and the energy transfer inhibitor oligomycin influenced these mitochondria in the well-known manner. The uncoupling activity of chlordimeform in vitro was very weak and decreased with increasing age of the eggs. Electron transport in mitochondria from chlordimeform-treated eggs was not uncoupled. Therefore, it is concluded that the ovicidal effect of this pesticide is not due to its uncoupling effect.  相似文献   

Fusarium solani Upregulated Sesquiterpene Synthase Expression,Sesquiterpene Production and Allelopathic Activity in Piper betle L.     

Kamrai Woranoot  Rattikarn Buaruang  Kodchakorn Aranyakanon  Kumrop Ratanasut  Anupan Kongbangkerd  Panatda Jannoey  Pranee Nangngam  Chonnanit Choopayak 《水稻科学》2019,26(5):290-299

Activators of sesquiterpene synthase (STS) gene expression and sesquiterpene production in Piper betle L. were examined using quantitative real time PCR and gas chromatography mass spectrometry methods, and the allelopathic activity of untreated and Fusarium solani-treated betel extracts was tested on seed germination and on the shoot and root growth of Thai rice variety PSL2 (Oryza sativa cv. Phitsanulok 2) and three dominant paddy weeds (Eclipta prostrata, Echinochloa crus-galli and Chloris barbata). The results demonstrated that F. solani dramatically upregulated STS expression and productions of β-cubebene, β-caryophyllene and germacrene D sesquiterpene when compared with the untreated control, and that betel extracts had a greater inhibitory effect on weeds than on rice. The effects were more clearly detected on seed germination and root growth than on shoot growth, and they were found to be dose-dependent. It is also noted that F. solani-treated extract had stronger effects than the untreated extract. The species most sensitive to the allelopathic effects was C. barbata, germination of which was completely inhibited even at a dose of 0.1 mg/mL untreated extract. With regards to rice, although betel extract at 1.0 mg/mL showed no inhibition on germination, it affected the elongation of rice roots, in addition to those of the tested weeds. The obtained data suggested that F. solani has potential as an activator of sesquiterpene allelochemical production via STS expression, the latter leading to the treated betel extract having a stronger phytotoxic effect. These results were beneficial in the promotion of natural herbicide production using biotechnology.  相似文献   

通信与网络技术在温室环境控制中的应用     

任玉灿  时玲 《农机化研究》2006,(11):179-180

由于现代科学技术的飞速发展,现代信息技术及网络技术逐渐在温室环境控制中得到了应用,智能化温室在世界范围内引起了越来越广泛的关注,并在全世界范围内开始普及。为此,论述了通信及网络技术在温室环境自动控制中的应用情况,由此可以看出智能化温室给设施农业的发展带来的深远影响。  相似文献   

北部湾浮游幼虫群落结构及其环境适应性分析          下载免费PDF全文

王学锋  李纯厚  廖秀丽  贾晓平 《上海海洋大学学报》2010,(4):529-534

根据1998年2月至1999年5月在北部湾海域按季度进行的4个航次生态、环境综合调查资料,本文研究了北部湾海域浮游幼虫的主要类群及其季节变动。结果表明:北部湾海域浮游幼虫主要有15大类群;其中4类(长尾类幼虫、短尾类幼虫、口足类阿利玛幼虫、蛇尾类长腕幼虫)周年出现,其它为季节性出现。长尾类幼虫、口足类阿利玛幼虫、蛇尾类长腕幼虫、短尾类溞状幼虫、短尾类大眼幼虫及其它短尾类幼虫为优势类群。北部湾浮游幼虫的年丰度变化范围为0.02~7.65ind/m³,均值为0.50ind/m³,四季的丰度为夏季(0.86ind/m³)>春季(0.40ind/m³)>秋季(0.32ind/m³)>冬季(0.12ind/m³)。从春季到冬季整个浮游幼虫密集中心呈逆时针从湾的东北部向西北部海区移动,移至湾的中部后再返至西北部。K优势度曲线分析表明群落多样性由高到低依次为春季>冬季>夏季>秋季,总体上浮游幼虫群落多样性的季节差异不大。典范对应分析结果表明影响浮游幼虫栖息密度的主要因子是水温和pH,其次是盐度、溶解氧。  相似文献