桃蚜电压门控钠离子通道基因cDNA克隆及其生物信息学分析          下载免费PDF全文

王颢  段文波  李芬  李为争  吴少英 《中国生物防治学报》2020,36(3):443-451

克隆获得桃蚜电压门控钠离子通道基因cDNA序列，明确钠离子通道的典型特征，为研究桃蚜抗性分子机理奠定基础。采用实验技术主要有RT-PCR和PCR，克隆桃蚜钠离子通道基因cDNA序列，利用相关软件对其序列进行生物信息学分析。克隆得到两段cDNA序列MpNa_v-1（NCBI登录号：MN124170）和MpNa_v-2（NCBI登录号：MN176136）。MpNa_v-1长度为2945 bp，包括2877 bp的完整开放阅读框，共编码958个氨基酸；MpNa_v-2长度为3546 bp，包括3486 bp的完整开放阅读框，共编码1161个氨基酸。MpNa_v-1和MpNa_v-2共同组成桃蚜的钠离子通道α亚基，MpNa_v-1包含同源结构域Ⅰ和同源结构域Ⅱ，MpNa_v-2包含同源结构域Ⅲ和同源结构域Ⅳ。同源比对发现，桃蚜与豌豆蚜和高粱蚜钠离子通道基因相似度分别高达97.67%和97.65%，所克隆序列包含昆虫钠离子通道α亚基典型特征，具有MFM模块，并含有蚜虫类钠通道特有模块DENS。成功地克隆桃蚜钠离子通道基因，为阐明其对拟除虫菊酯类药剂产生靶标抗性的分子机制奠定基础。  相似文献   

铜对生长猪肝中IGF—Ⅰ基因mRNA表达的影响     

郑鑫刘国文  杨连玉李家奎夏清娟  郝德威王哲 《中国兽医科技》2006,36(6):497-501

采用生长试验、免疫放射分析和定量PCR方法，观察了生长猪血液中类胰岛素生长因子（IGF—Ⅰ）及其结合蛋白（IGFBP3）数量的变化和IGF—Ⅰ基因在肝中表达量的变化。结果显示，每1kg饲料中添加125-250mg铜，能够上调IGF-Ⅰ基因的表达量，显著提高猪的平均日增重，促进生长猪循环血液中IGF—Ⅰ浓度的增加。试验结果证实，铜是通过促进生长激素一胰岛素样生长因子轴相关因子的合成和分泌来发挥促生长作用的。  相似文献   

雏鸭铜中毒的实验观察   总被引：14，自引：4，他引：10  

崔恒敏  陈怀涛 《中国家禽》2003,25(11):8-10

1日龄天府肉鸭健雏210只随机分为三组,分别喂给铜12.16mg/kg(对照或1组)、850mg/kg(2组)和1050mg/kg(3组)日粮7周。结果表明,后两组雏鸭分别于3周龄和2周龄开始出现临床症状,生长发育迟缓,并出现死亡;血红蛋白含量和红细胞数量显著低于对照组(p<0.05),红细胞变形、变性,大小不等;血清铜含量和肝铜含量极显著高于对照组(p<0.01)。  相似文献   

日粮中添加铜对高产乳牛泌乳量影响的研究   总被引：3，自引：0，他引：3  

黄志秋  何学谦  刘利春  徐大勇  侯成万 《四川畜牧兽医》2001,28(11):22-22,24

本研究探索了在奶牛日粮中添加Cu++对其产奶性能的影响.结果表明,在日粮中添加Cu++ 7.6×10-6,牛奶的乳脂率和干物质含量并未降低,牛奶中铜的残留量低于国家规定标准,能使奶牛泌乳量增产7.66%～9.61%,从而增加乳牛业的经济效益.  相似文献   

不同钠浓度对白三叶草和多年生黑麦草生长的影响     

彭克勤  胡笃敬 《湖南农业大学学报(自然科学版)》1993,(2)

白三叶草对Na 的反应比多年生黑麦草敏感.培养液中的Na 浓度从0.1mmol/L 增至0.5mmol/L 时,其干物质生产下降37%;增至2.5mmol/L 时,干重下降49%,而多年生黑麦草的干重仅降低6%.2种牧草的Na 含量均随培养液Na 浓度的增加而提高,K 含量未受影响.白三叶草的Na 含量低于多年生黑麦草,而K 含量高于多年生黑麦草.试验结果暗示,白三叶草对K 的选择吸收能力较多年生黑麦草强,但耐盐能力较差,生态适应性不如多年生黑麦草广.  相似文献   

单次肌注亚硒酸钠在仔猪体内的药代动力学     

赵晶  康世良  李艳华  陈辉 《中国兽医学报》2003,23(2):188-190

按 0 .2 m g/ kg单剂量对健康仔猪肌注亚硒酸钠溶液后 ,研究了其在血液中的药物代谢动力学。结果表明 :血液动力学特征符合一级吸收二室开放模型 ,其理论方程为 :C=0 .1773e- 0 .0 548t+0 .0 72 7e- 0 .0 0 2 6 t- 0 .2 5 0 0 e- 1 3.882 3t。主要动力学参数为 :吸收半衰期 (t1 /2 Ka)为 0 .0 4 99h;达峰时间 (tmax)为 0 .4 2 37h;消除半衰期 (t1 /2β)为 2 71.9311h;曲线下面积 (AU C)为 31.72 6 0 m g/ L·h;表观分布容积 (Vd)为 2 .4 72 1L/ kg。根据单剂量药动学参数 ,计算多剂量给药参数 ,为临床治疗制定给药方案。先导剂量 (D* )为 0 .5 0 89m g/ kg,维持剂量 (D0 )为 0 .2 mg/ kg,给药间隔 (τ)为 192 h,平均稳态血药浓度 (c)为 0 .16 5 2 mg/ L。  相似文献   

维生素K3的合成及应用   总被引：5，自引：0，他引：5  

彭莉 《中国兽药杂志》2003,37(10):50-52

本文概述了维生素K3的各种合成技术路线，对维生素K3生产技术的发展进行了展望，介绍了其应用和市场供求现状。  相似文献   

Application and crop safety parameters for soil fumigants   总被引：2，自引：0，他引：2  

A. S. Csinos  T. M. Webster  D. R. Sumner  A. W. Johnson  C. C. Dowler  K. W. Seebold 《Crop Protection》2002,21(10):973-982

Metam sodium alone and in combination with 1,3-dichloropropene plus 17% chloropicrin (1,3-D+C-17) were evaluated under polyethylene mulch film as alternatives for methyl bromide in tobacco and tomato transplant production for both efficacy against pests and crop safety. Eight different weed species, 10 genera or species of fungi and several agronomic criteria were evaluated at three different sites. In general both the metam sodium alone and in combination with 1,3-D+C-17 were highly efficacious when compared to methyl bromide. Short polyethylene film retention times and short aeration times resulted in poor stands and poor crop vigor while relatively long polyethylene film retention times and long aeration periods at the same rates typically resulted in high stand counts and vigor. Combination treatments were more phytotoxic to germinating seed of tobacco and tomato. Vigor and stand counts of the seedlings were higher as aeration time increased, suggesting phytotoxic residues dissipate with time. Method of application of metam sodium, either injected with chisels or sprayed onto the soil surface and incorporated with a tractor-powered tiller alone or co- applied with 1,3-D+C-17 chisel injected, did not affect the efficacy of the treatments. Caution regarding phytotoxicity must be exercised when seeding into soil fumigated with metam sodium alone or combined with 1,3-D+C-17. Additional work will be required to establish safety periods required prior to transplanting crops into fumigated soil.  相似文献   

Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation     

G. Tuitert  Y. Hofmeester 《European journal of plant pathology / European Foundation for Plant Pathology》1994,100(1):19-53

A field experiment was set up in 1988 to study the development of rhizomania disease of sugar beet at different inoculum levels of beet necrotic yellow vein virus (BNYVV) in soil. Five, tenfold different, inoculum levels were created by addition of the approximate amounts of 0, 0.5, 5, 50 and 500 kg infested soil per ha (the latter corresponding to 0.01% v/v calculated to the tillage layer). A drip irrigation treatment was applied to study the influence of soil moisture on disease. Susceptible sugar beet, cv. Regina, was grown for three consecutive years.In the first year, root symptoms were not observed, but BNYVV-infected plants were detected by ELISA in low numbers at all inoculum levels at harvest. After late drilling in 1989, high numbers of infected plants, up to 90–100% in plots with the highest inoculum level, were detected already in June. Root symptoms were also observed from June onwards. In both these years disease incidence increased in time and was significantly influenced by the initial inoculum level. In the third year, the whole field was heavily diseased, and only for the non-irrigated plots incidence differed for different initial inoculum levels. The expression of symptoms by BNYVV-infected plants was influenced by initial inoculum level, thus by the amount and timing of primary infection.Root weight at harvest was not affected, but sugar content decreased with increasing inoculum level already in 1988, leading to a reduction in sugar yield of 10% at the highest inoculum level. In 1989, both root weight and sugar content decreased progressively with increasing inoculum level, resulting in sugar yield reductions of 11–66% (down to approximately 3000 kg ha^–1) for low to high inoculum levels compared to the control. As the control plots became contaminated, all yields were low in 1990, still showing a decrease with increasing inoculum level in the non-irrigated plots, but an overall mean sugar yield of 3323 kg ha^–1 for the irrigated ones.Sodium and -amino nitrogen content of the root, additional quality parameters determining extractability of sucrose, showed an increase and decrease, respectively, with increasing initial inoculum level already in the first year. The relative differences in contents compared to those from the control were largest for Na content. A significant negative correlation was found between Na (mmol kg^–1 root) and sugar content (% of fresh weight); linear for 1988, exponential for 1989 and 1990.In spring 1989, the infestation of individual plots was assessed using a quantitative bioassay estimating most probable numbers (MPNs) of infective units of BNYVV per 100 g dry soil. The relationship between the MPns determined and root weight, sugar content and sugar yield at harvest could be described by Gompertz curves. The increase in disease incidence with increasing MPN in 1989 was adequately fitted with a logistic equation.  相似文献   

Annual bluegrass (Poa annua L.) control in bentgrass (Agrostis palustris Huds.) green with sequential application of bispyribac‐sodium combined with dinitroanilines*     

NAM‐IL PARK  YUKO SUTO  YUTAKA MIURA  NOBUO NAKATANI  SHIN‐ICHI IORI  MASARU OGASAWARA 《Weed Biology and Management》2002,2(3):159-162

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