SSR技术及其在果树上的应用   总被引：27，自引：4，他引：27  

高志红  章镇  韩振海 《果树学报》2002,19(5):281-285

SSR(Simple sequence repeat)技术以其丰富的多态性、共显性遗传、重复性好和操作简便等优点日益受到重视,已成为植物遗传和育种研究中不可缺少的分子标记。对SSR技术的原理和特点作了简要的介绍,较详细地分析了如何获得SSR引物,特别是综述了果树上SSR引物的研究现状,同时将其与其它几种主要的分子标记进行了比较分析,认为SSR标记检测的位点多态性水平明显高于RFLP,而且重复性优于RAPD;着重介绍了SSR技术在果树种质资源和构建果树遗传图谱及基因定位等研究中的应用现状;指出SSR技术将在果树科研上起到重要的作用。  相似文献   

草莓叶面积简易测定方法   总被引：16，自引：1，他引：16  

乔宝营  黄海帆  张信栓  马彩霞  李道德 《果树学报》2004,21(6):621-623

以透明方格法作对照,分别与叶面积的其他简易测定法(叶长度×叶宽度的系数法、叶长度与叶面积的回归法、叶宽度与叶面积的回归法)比较。结果表明吐德拉(Tudla)叶面积的每种测定方法,以叶宽度与叶面积回归法最精确,计算公式是=7.0918x-13.4784穴公式中的x是叶宽度,y是叶面积,以下相同雪;鬼怒甘(Kinuama)叶面积的测定法也是叶宽度与叶面积回归法最精确,公式是=7.3187x-14.3288。杜克拉(Dukela)叶面积的测定法,推荐用叶宽度与叶面积的回归法,公式是=6.6393x-10.6411。y=a+bx^y=a+bx^y=a+bx^  相似文献   

Transgenic expression of a sorghum gene (SbLRR2) encoding a simple extracellular leucine-rich protein enhances resistance against necrotrophic pathogens in Arabidopsis     

《Physiological and Molecular Plant Pathology》2015

Plant leucine-rich repeat (LRR) domain-containing proteins are known to play important roles in signaling transduction and defense responses. In sorghum, SbLRR2 is pathogen-inducible gene encoding a simple extracellular LRR protein. Here, we demonstrated an earlier and stronger expression of SbLRR2 in a sorghum resistant genotype in comparison to a susceptible genotype following inoculation with the anthracnose pathogen (Colletotrichum sublineolum). In addition, SbLRR2 expression was found to be induced strongly by methyl-jasmonate treatment. Functional analysis was performed in SbLRR2 over-expression (OE) Arabidopsis plants, which showed enhanced resistance against the necrotrophic pathogens Botrytis cinerea and Alternaria brassicicola. In addition, the OE lines were found to have elevated expression of several jasmonate acid (JA)-associated genes and higher endogenous JA contents. Hence, the SbLRR2-mediated defense responses in transgenic Arabidopsis are likely to be dependent on JA-signaling through increased JA production. On the other hand, the OE lines remained susceptible to Pseudomonas syringae pv. tomato like the wild type plants. Consistently, there was no up-regulation of salicylic acid (SA) defense marker gene expression or SA levels in the OE lines. Our results suggested that SbLRR2 is potentially useful for enhancing resistance against necrotrophic pathogens in transgenic dicot crops.  相似文献   

停泵水锤的简易算法     

罗伟  陈坚 《排灌机械》2005,23(2):16-19

针对目前国内外使用广泛的各种水锤简易算法，进行归纳比较和对照，并结合工程实例，采用多种算法分析；最后对各种算法的优缺点和适用条件进行了比较，以便得出有效的计算方案。  相似文献   

植物EST-SSR标记开发及其应用     

张利达  唐克轩 《广西农业生物科学》2010,(3):534-541

随着生物科技的进步,大量的植物表达序列标签（expressed sequence tags,ESTs）已经成为开发SSR标记的重要资源。EST-SSR作为一种新型分子标记,其多态性可能与基因功能直接相关,而且在相近植物间具有良好通用性,使得EST-SSR标记在实际应用中更具价值。采用计算机方法大规模发掘EST-SSR多态性位点极大地提高了SSR标记开发效率。尤其随着新一代测序技术的成熟以及测序成本的急剧下降,利用新一代测序技术产生大量的转录组数据进行EST-SSR多态性标记的计算机大规模发掘将对SSR标记的开发带来深远影响。本文简要介绍了植物EST-SSR分布特点,并对EST-SSR标记开发现状以及相关应用作了综合评述,此外,还对EST-SSR标记的计算机开发新策略进行了展望。  相似文献   

根据土壤水平一维入渗推求紫色土水动力学参数   总被引：1，自引：0，他引：1  

程冬兵  张平仓  李亚龙  蔡崇法 《中国生态农业学报》2010,18(5):1007-1012

选择3种不同质地的紫色土,通过室内土壤水平一维入渗试验,探讨简单入渗法和入渗特性法推求水动力学参数对于紫色土的适用性。结果显示,简单入渗法和入渗特性法推求的水动力学参数中,水分特征曲线准确性较高,水分扩散率和非饱和导水率准确性较差。简单入渗法推求的水分扩散率和非饱和导水率均低于实测值1～2个数量级,但推求值与实测值曲线趋势较为一致。入渗特性法推求的水分扩散率和非饱和导水率在高含水量小于实测值,在低含水量大于实测值,推求值随含水量变化趋势响应缓慢。  相似文献   

Nomenclature and genetic relationships of apples and pears from Terceira Island     

Nahla Bassil  Kim E. Hummer  Joseph D. Postman  Gennaro Fazio  Angela Baldo  Isabel Armas  Roger Williams 《Genetic Resources and Crop Evolution》2009,56(3):339-352

Heritage apple (Malus domestica Borkh. hybrids) and pear (Pyrus communis L. hybrid) trees grow in villages throughout Terceira Island, Azores, Portugal. Some of these pears have different names but similar morphology. The objective of this study was to determine synonymy, homology, and phylogeny of apples and pears from Terceira and to examine potential relationships of the island pears with standard apples and pears of Portuguese or American descent. Nine apple microsatellite markers were used to determine genetic relationships. Distance- and parsimony-based cluster analysis grouped these genotypes into separate apple and pear clades. The Terceira apples were divided into two clades: the maçā and the reineta-reinette. Among the 17 heritage apple genotypes, seven unique accessions were identified and four groups of synonyms, or possibly clones, were detected including: ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares; ‘Reineta Castanha’ and ‘Reineta Verde Miuda’; ‘Maçā Pêra,’ ‘Maçā Calhau’, ‘Pêro Branco’ from Salga and from Terra-Chā and ‘Maçā Marmelo’; and the five genotypes ‘Maçā Sao Joao’, ‘Malápio Rosa’, ‘Maçā Gaspar’, ‘Maçā Branca’ and ‘Maçā Pato’. In addition, two homonyms were detected. ‘Pêro Vermelho’ from Terra Chā was a separate genotype from a tree from Doze Ribeiras of the same name, but Pêro Branco from Terra Chā appears to be a clone that can be distinguished by an additional allele at CH1F07a from a tree with that name from Salga. One pair of apple clones, ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares appear to be derived from an unreduced gamete of ‘Golden Delicious.’ Another apple genotype ‘Maçā Acida’ could be a sibling of the ‘Maçā Pêra’ clonal group. Other tested standard apples from the US genebank were unrelated to Terceira genotypes. Of the seven heritage pears, five unique genotypes and one pair of synonyms were detected. ‘Pêra Papo Pintassilgo’ from Raminho and ‘Pêra Vermelha’ from the nursery of Serviço de Desenvolvimento Agario da Terceira (SDAT) were synonyms. ‘Passans du Portugal’ was related to ‘Pêra Cabaca’ but other standard pears from the US genebank were unrelated to Terceira genotypes. Future studies will include additional apple and pear cultivars from other Islands of the Azores and continental Portugal, and wild Asian species to further explore genetic relationships.  相似文献   

基于GOES数据和弱约束变分的地表水热通量估算     

刘翔舸  黄健熙  秦军  王鹏新  徐同仁 《农业机械学报》2014,45(1):236-245

基于弱约束的四维变分方法和陆面过程模式发展了一个地表温度的陆面数据同化系统。本文反演了地球静止业务环境卫星(Geostationary operational environmental satellite,GOES)的地表温度,并将反演的地表温度同化入陆面过程模式,改进陆面过程模式中地表水热通量的估算精度。以弱约束的变分方法通过在代价函数中增加弱约束项代替陆面过程模式动力方程组中存在的模式误差,构建新的代价函数并对其优化,从而改善模式中显热与潜热的估算精度。将GOES地表温度与实测地表温度进行比较,其均方根误差(RMSE)作为试验中的观测误差。选择美国通量网AmeriFlux中2个主要农业站点的气象和通量数据作为试验数据,对同化系统进行驱动和验证。结果表明同化后的地表温度、潜/显热估算精度均有提高。其中,各站地表温度RMSE平均仅为1 K,显热通量平均RMSE下降22 W/m2,潜热通量平均RMSE下降26 W/m2。因此结合陆面过程模式的弱约束变分方法同化GOES反演温度产品估算近地表水热通量的方法是有效且可行的。  相似文献   

60个玉米自交系的SSR标记分析   总被引：5，自引：0，他引：5  

李凌雨  孟全业  王学雄  闫彩清  王圆荣  马玉光 《华北农学报》2007,22(3):25-29

利用SSR标记研究了60个山西省主要玉米(Zea maysL.)自交系的遗传变异。用23对扩增带型稳定的引物,从供试自交系中检测出91个等位基因变异,每对引物检测出等位基因2~7个,平均为3.96个,平均多态性信息含量为0.600。UPGMA聚类分析将60个自交系划分为唐四平头,旅大红骨,PN,BSSS,Lancaster,Suwan等6个类群,化群结果与其系谱关系和育种家经验基本一致。  相似文献   

小豆SSR-PCR反应条件的优化   总被引：3，自引：0，他引：3  

丁燕红  金文林  赵波  濮绍京 《北京农学院学报》2006,21(1):14-17

笔者对小豆SSR-PCR扩增体系的反应条件进行了优化,分析了Mg2+浓度、dNTP浓度、DNA模板浓度、引物浓度、Taq聚合酶浓度对PCR扩增结果的影响。结果表明:在10 μL的PCR反应体系中,Mg2+的最适浓度为2 mmol/L,dNTP最适浓度为0．25 mmool/L,反应体系中Taq聚合酶宜加入1 U,引物的最适浓度为 0．15 mmol/L,DNA模板加入量为20 ng。  相似文献