猪钙蛋白酶抑制蛋白基因的多态性分析   总被引：4，自引：0，他引：4  

杨晓慧  刘源  武英  樊新忠  姜运良 《中国畜牧杂志》2006,42(19):8-9

钙蛋白酶抑制蛋白的638位氨基酸由丝氨酸(Ser)突变为精氨酸(Arg)可改善猪的肉质,是一个有重要育种应用前景的分子标记。本研究采用PCR-RFLP技术对47头莱芜猪,53头大白猪,45头杜洛克和60头商品杂交猪共205个个体钙蛋白酶抑制蛋白Ser638Arg突变的多态性进行了分析。结果表明:4个猪群均检测到AA、CA和CC3种基因型。其中莱芜猪突变等位基因A频率最低,为0.415;大白猪、杜洛克和商品杂交猪A的频率分别为0.632、0.800和0.750。莱芜猪A的基因频率极显著低于其他4个猪种(P<0.01)。所检测的4个猪群在该突变位点均处于Hardy-Weinberg平衡状态(P>0.05)。  相似文献   

Phenotypic and genetic characterization of Erwinia carotovora from mulberry (Morus spp.)     

S. T. Seo  N. Furuya  C. K. Lim  Y. Takanami  K. Tsuchiya † 《Plant pathology》2003,52(2):140-146

Phenotypic and genetic characteristics of nine bacterial strains isolated from mulberry ( Morus spp.), which were originally described as Erwinia carotovora ssp. carotovora (Ecc), were investigated. Based on the results of biochemical tests, these bacterial strains were divided into two different types, type 1 and type 2. Two strains of type 1 were similar to Ecc, whereas seven strains of type 2 were distinct from Ecc. A polyphasic study that included serological assay, specific PCR assay for E. carotovora ssp. atroseptica (Eca), PCR-RFLP of a pectate lyase ( pel ) gene and RAPD-PCR was performed on the type 2 strains, and the data were compared with those of related E. carotovora subspecies. The results of serological and specific PCR assays for Eca showed that the type 2 strains were distinct from Eca. In RFLP analysis of the pel gene using Sau 3AI, the type 2 strains showed a unique RFLP pattern. On the basis of RAPD analysis, similarity of RAPD patterns within the type 2 strains was very high. A unique RAPD fragment was isolated from the type 2 strains and used as a probe for Southern hybridization. This probe hybridized only with PCR products from the type 2 strains. Based on phenotypic, serological and genetic characteristics, the type 2 strains isolated from mulberry may belong to a distinct E. carotovora subspecies other than Eca or Ecc.  相似文献   

昆虫病原线虫rDNA多态性分析   总被引：1，自引：0，他引：1  

丘雪红韩日畴  许再福 《昆虫天敌》2003,25(3):130-144

本文对国内外昆虫病原线虫斯氏属和异小杆属的47个品系进行rDNA—ITS PCR—RFLP分析，研究其DNA多态性，并构建了分子系统发育树状图。各品系的ITS区无明显的长度差异，PCR—RFLP分析将47个品系分为斯氏属和异小杆属两大类，两属线虫又分别分为11组和4组。所得结果丰富了ITS PCR—RFLP图谱库，为弄清我国的昆虫病原线虫与国外种类的分子系统发育关系及未定名线虫的鉴定提供重要依据，同时为筛选适合的线虫种类防治害虫奠定基础。  相似文献   

对鳞翅目害虫有活性的cry1C基因的克隆和表达   总被引：2，自引：0，他引：2       下载免费PDF全文

宋福平  张杰  韩岚岚  高继国  黄大昉 《植物保护学报》2003,30(2):161-165

在鉴定苏云金芽孢杆菌(Bacillus thuringiensis，简称Bt)Btc001菌株cry基因型的基础上，构建了Btc001菌株质粒DNA HindⅢ片段的文库，并利用聚合酶链式反应-限制性酶切片段多态性(PCIR-RFIP)方法筛选出含有crylC6全长基因的13．5kb大片段，酶切分析得到该片段的物理图谱，BamHI和EcoRI切完成了6．5kb含全长基因的亚克隆，并对这条6．5kb片段亚克隆、测序，序列在国际核酸序列数据库(GenBank)登记(AY007686)，并由Bt杀虫晶体蛋白基因国际命名委员会命名为cry1Cb2基因。根据序列设计了一对用于扩增全长基因的引物S581CB和S381CB，扩增产物插入表达载体pET-21b中，诱导后在大肠杆菌BL21(DE3)中获得高效表达。表达产物对小菜蛾(Plutella xylostella)表现出较高活性，LC50达到7．9μg/ml。  相似文献   

新扬州鸡IGF-1基因多态性与早期生长速度关系的研究   总被引：9，自引：2，他引：9  

王志跃  范刚  杨海明  汪张贵  盛东风  刘桂琼 《中国家禽》2004,26(24):9-12

以150只非同胞新扬州鸡为材料，采用PCR—RFLP法检测了该基因5’调控区DNA序列多态性，并运用线性模型统计方法分析了多态性与初生重和12周龄体重的关系。结果显示：新扬州鸡IGF-1基因5、调控区自然存在两种不同DNA序列，经。PstⅠ酶切后出现3种基因型(“-／-”、“-／ ”、“ ／ ”)，基因型分布符合哈代一温伯格定律。各基因型个体的初生重、12周龄体重的最小二乘均数存在“-／-”＞“ ／-”＞“ ／ ”的趋势，且“-／-”型个体的12周龄体重显著高于“ ／ ”型个体(P＜0．05)。  相似文献   

西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析   总被引：6，自引：0，他引：6  

张润锋  陈宏  蓝贤勇  田燚  潘传英  胡沈荣  苏利红 《畜牧兽医学报》2005,36(6):545-549

应用PCR-RFLP方法对西安荷斯坦牛的κ-en、β-lg、β-lg5′侧翼区、CSN1S2、IGFBP-3共5个基因座位进行了多态性分析。结果表明，在西安荷斯坦牛群中，没有发现携带CSN1S2^P等位基因的个体，其多态信息含量为0。κ-en基因座位呈现低度多态(PIC=0．2366)，β-lg、β-lg5′侧翼区、IGFBP-3基因座位的多态信息含量分别为0．3168、0．3689、0．4439，均呈现中度多态。κ-en、β-lg、β-lg5′侧翼区、IGFBP-3、CSNIS2基因座位的杂合度和DNA多态度分别为0．2742、0．3947、0．4879、0．4891、0和0．0255、0．0116、0．0333、0．0112、0。而且，在西安荷斯坦牛群中，κ-en、β-lg、β-lg5′侧翼区、IGFBP-3共4个基因座位均处于Hardy-Weinberg平衡状态，CSN1S2基因座位处于纯合状态。  相似文献   

铅锌矿区重金属污染对微生物数量及放线菌群落结构的影响     

李小林  颜森  张小平  韦成健 《农业环境保护》2011,(3):468-475

采集四川省汉源县富泉乡万顺铅锌矿区5个不同重金属浓度的土壤样品,进行了微生物数量及放线菌多样性的研究。经分离、纯化得到43株不同的放线菌,然后对其进行BOXAIR-PCR和16SrDNAPCR-RFLP分析。结果表明,铅锌矿区重金属复合污染对土壤微生物数量有较大的影响,随着铅锌矿区重金属污染程度的加剧,土壤微生物的总数下降。相关性分析表明,重金属含量与细菌数量呈极显著负相关（P〈0.01）,与放线菌数量、真菌数量呈显著负相关（P〈0.05）。供试菌株的16SrDNA用HaeⅢ、HinfⅠ和TaqⅠ酶切后具有32种遗传图谱类型。BOXAIR-PCR的聚类结果表明在86%的水平上,所有菌株分为10个遗传类型,结果基本与16SrDNAPCR-RFLP聚类差异不大。来源于高重金属的含量样品的菌株基本聚在一起,可能是重金属含量影响了放线菌的分布。同时,16SrDNA序列聚类分析结合系统发育树分析表明链霉菌属是汉源铅锌矿区主要的放线菌属并且具有遗传多样性。  相似文献   

黑龙江凉水自然保护区苏云金芽孢杆菌的收集与鉴定     

张文飞  ;谢柳  ;赵立仕  ;方宣钧  ;柳参奎 《广西农业生物科学》2009,(4):685-690

黑龙江凉水自然保护区是我国现有保存下来的较大片原始红松林基地之一,总面积6394hm^2,森林覆盖率95％以上。本研究从小兴安岭凉水自然保护区采集土样782份,采用醋酸钠培养基结合高温方法筛选土壤中的芽孢杆菌,通过光学显微镜观察鉴定产生伴胞晶体的苏云金芽孢杆菌（Bacillus thuringiensis．Bt）。总计分离得到芽孢杆菌和苏云金芽孢杆菌分别为1735株和33株,Bt菌株的分离率和出菌率分别为1．90％和4．22％。利用SDS-PAGE和PCR—RFLP方法对筛选获得的成分离株进行了杀虫晶体蛋白和基因型分析,结果表明14株产菱形伴胞的＆菌株,SDS-PAGE电泳分析芽孢后期产生分子量大小130kD蛋白带,PCR-RFLP分析初步鉴定为crylAc基因,其它产圆形或其它不定形晶体蛋白的＆分离菌中,芽孢后期主要蛋白大小为20～150kD不等,PCR—RFLP方法鉴定结合PCR片段测序分析这些菌株含有新型cry4、cry39和cry40基因等。本研究是“中国助资源收鉴与利用”项目组成部分之一,凉水自然保护区苏云金芽孢杆菌的收集与鉴定目的是要对整个东北地区成资源分布和多样性作一个初步评估,实验结果表明东北森林地区具有丰富多样的威菌株和杀虫基因资源。  相似文献   

Attaining inter-subgeneric hybrids in fragrant azalea breeding and the inheritance of organelle DNA   总被引：1，自引：0，他引：1  

Nobuo Kobayashi  Daiki Mizuta  Akira Nakatsuka  Masaru Akabane 《Euphytica》2008,159(1-2):67-72

Inter-subgeneric hybrids were successfully obtained in reciprocal cross combinations between evergreen azaleas (Rhododendron nakaharae and its hybrids) and fragrant deciduous azaleas (R. arborescens and R. viscosum) for the purpose of fragrant evergreen azalea breeding. Nuclear and organelle DNA of these hybrids was investigated using PCR-RFLP markers. Viable hybrid seedlings have nuclear ribosomal DNA (nrDNA) inherited biparentally, mitochondrial DNA (mtDNA) from the seed parent, and chloroplast DNA (cpDNA) from the deciduous azalea, regardless of cross combination. These results suggest that the chloroplast genome from deciduous azaleas and the nuclear genome from evergreen azaleas are compatible in viable hybrid progenies.  相似文献   

Molecular phylogeny in Indian Citrus L. (Rutaceae) inferred through PCR-RFLP and trnL-trnF sequence data of chloroplast DNA     

Satya Narayan Jena  Susheel KumarNarayanan K. Nair 《Scientia Horticulturae》2009

The taxonomy and phylogeny of Indian Citrus is revisited using PCR-RFLP of the trnD-trnT and rbcL-ORF 106 regions as well as sequence data analysis of the trnL-trnF intergenic spacer region of cpDNA. The study was based on 50 accessions of Citrus genotypes, collected from wild, semi-wild and domesticated stocks. Of the 13 restriction enzymes (RE) used for restriction digestion of the polymerase chain reaction (PCR) amplicons, four (Hinf I, Msp I, Alu I, Hae III) generated 47 restriction fragments, of which 24 (51%) were polymorphic. PCR-RFLP data showed a genetic distance ranging from 0 to 0.79 among 50 accessions of Citrus, and a cluster analysis, based on Neighbor-Joining (NJ) method, placed all the accessions in eight major clusters. Analysis of trnL-trnF sequences from 23 representative accessions of Citrus showed a pair-wise sequence divergence rate in the range of 0–0.064. NJ, minimum evolution (ME) and maximum parsimony (MP) analyses of trnL-trnF sequences produced phylogenetic trees, which placed all the 23 accessions in five clusters. PCR-RFLP analysis resulted in a well resolved phylogenetic tree with branches supported by moderate to high bootstrap values, while the trnL-trnF sequence-based trees showed only moderate to low bootstrap support for the internal tree branches, indicating uncertain origin of some Citrus genotypes. This study shows that the trnL-trnF spacer sequence data can detect genetic variation in Indian Citrus genotypes, but the utility of the data in inferring phylogeny at intra and inter-specific levels is limited probably by factors such as hybridization, bud mutations, apomixis and polyploidy. However, PCR-RFLP and trnL-trnF data supported the recognition of C. maxima, C. medica, and C. reticulata as the basal species of edible Citrus.  相似文献