鲤春病毒血症病毒(SVCV)的研究进展   总被引：4，自引：1，他引：4       下载免费PDF全文

付峰  刘荭  黄倢  蔡生力 《中国水产科学》2006,13(2):328-334

鲤春病毒血症病毒（SVCV）是一种能够引起鲤科鱼类急性传染性病毒病-鲤春病毒血症（SVC）的病原，一旦暴发会造成巨大的经济损失。该病主要在欧洲的鲤鱼养殖中广泛传播，主要症状为体内出血，腹膜炎及腹水。SVCV具有囊膜，暂时列为弹状病毒科水泡性病毒属。病毒基因组为线性单股不分段的负链RNA，长11019个核苷酸，主要包含5个基因，分别编码核蛋白N、磷蛋白P、基质蛋白M、糖蛋白G和RNA聚合酶L。日前，中国出入境检验检疫检测SVCV的行业标准是逆转录PCR法。本研究从病毒的生物学特征，感染的临床症状及流行特点，病毒基因组及其多肽特征，病毒的分类地位以及检测技术等方面对国内外研究的现状作一综述，以期为研究者提供理论参考。  相似文献   

鲤鱼单极虫病组织病理研究   总被引：1，自引：0，他引：1  

肖国华  张卫平 《河北渔业》1992,(4):14-17

本文报道了1989年以来,造成王快水库等网箱养鲤大批死亡的单极虫病,病鱼出现烂鳃、肛门红肿、腹部膨大,腹腔内有大量粘液等症状;显微观察的结果为:各组织器官发生病变,肠上皮细胞严重坏死,平滑肌纤维发生玻璃样变及解体,鳃丝充血,鳃小片融合,肝细胞核溶解、坏死。  相似文献   

草鱼肾组织细胞系CIK的建立及其生物学特性   总被引：20，自引：3，他引：20  

左文功  钱华鑫  许映芳  杜森英  杨先乐 《水产学报》1986,10(1):10-17

作者于1982年1月开始进行草鱼肾组织单层细胞培养，至今已连续培养32个月，传至120多代，建立了细胞系，定名为草鱼肾组织细胞系(CIK)。本文介绍了原代和传代培养、细胞形态、细胞生长速度和分裂指数、细胞的保存和对温度的适应性、细胞染色体分析以及细胞系对病毒敏感性的试验和研究结果。CIK生长迅速、适应性强、以20℃-38℃生长较好，28℃左右生长稳定，pH为6．5时仍能保持致密单层，染色体数为非整倍体，众数为55。对草鱼出血病左右病毒FRV具敏感性。  相似文献   

锦鲤暴发性出血症病原的研究   总被引：6，自引：0，他引：6  

莫介化  李春枝  张邦杰  黄永强  李本旺 《水产科技情报》2002,29(6):280-283

从患暴发性出症的锦鲤体内分离出2株细菌－052202和052203，应用细菌分离鉴定方法和法国Biomerieux FRC公司的API全自动微生物鉴定系统进行鉴定，结果显示：052202株为嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia),052203株为温和气单胞（菌（Aeromonas sobria)。生物毒性试验的结果表明，温和气单胞菌可使健康锦鲤死亡，引起体表出血，肝，肾，脾，胆囊发生病变，有致病性；而嗜麦芽窄食单胞菌不引起健康锦鲤死亡，无致病性，28例药物的药敏试验结果表明，温和气单胞菌对新霉素，卡那霉素，庆大霉素，头孢噻吩，头孢曲松的头孢他啶的敏感性强或较强。  相似文献   

Influence of nutrition on the lipid quality of pond fish: common carp (Cyprinus carpio) and tench (Tinca tinca)     

Werner Steffens  Manfred Wirth 《Aquaculture International》2007,15(3-4):313-319

Like marine fish freshwater fish are an important source of essential fatty acids for human nutrition. However, the fatty acid composition of pond fish can vary considerably and strongly depends on that of the ingested food. Investigations on the fatty acid composition of common carp (Cyprinus carpio) and tench (Tinca tinca) have shown that different methods of rearing and feeding cause substantial variations in the proportions of the n-6 and n-3 polyunsaturated fatty acids of these fish species. Carp reared on the basis of natural food in ponds exhibit high contents of n-6 as well as n-3 fatty acids in their muscle triacylglycerols. On the other hand carp fed supplementary wheat in ponds resulted in somewhat lower levels of these essential fatty acids. High amounts of n-3 fatty acids can be found in carp fed high-energy diets containing high levels of fish oil. Analogous results were obtained in experiments with tench reared under different nutritional conditions. While rearing on the basis of only natural food in ponds as well as feeding supplementary wheat yielded in similar levels of n-3 and n-6 polyunsaturated fatty acids, higher contents of n-3 fatty acids were recorded in tench fed pellets. High levels of n-3 polyunsaturated fatty acids in foodstuffs have positive effects on human health. Experiments with different cultured fish species proved that the fatty acid composition of the edible parts can be influenced by the diet. Therefore, a finishing diet with a suitable fatty acid profile can be used to improve the nutritional quality of fish products of farmed origin.  相似文献   

Dietary essentiality of ascorbic acid in rohu larvae: Quantification with ascorbic acid enriched zooplankton   总被引：2，自引：0，他引：2  

Gopa Mitra  P.K. Mukhopadhyay 《Aquaculture International》2003,11(1-2):81-93

Carp larvae, like any other fish larvae dependon natural food during first few days of theirlife. In nursery conditions, high mortality andslow larval growth are of common occurrence;sub-optimal nutrition might be a possiblereason for such consequences. To improve thesituation the effect of feeding ascorbicacid-enriched live food on survival, growth,tissue biochemical composition includingascorbate level was evaluated in first feeding(3 days old) larvae (av. wt. 2.2 mg) of therohu carp, Labeo rohita (Ham.) for aperiod of 15 days (temp. 28.6 ± 1 °C)under natural photoperiod. The larvae (stockingdensity 10 l^–1) were offered enriched andnon-enriched zooplankton ad libitumfollowing a rigid schedule with four feedingregimes, each having 3 replicates. In treatmentT₁, non-enriched zooplankton (Moina,Daphnia, Cyclops, Diaptomus) and in T₂,T₃, T₄ ascorbic acid enriched (12 henrichment) zooplankton [@10%, 20% and 30%ascorbyl palmitate (AP) inclusion in diet ofzooplankton] were offered. Highest survival(90%) and growth (9563% live weight gain)could be seen in T₃ group and the lowestin T₁ (62% survival and 805% live weightgain), thus confirming the dietary essentialityof ascorbic acid for rohu larvae. Therequirement has been shown to be 1409 µg/gdry diet. Whole body tissue analyses for crudeprotein, total lipid and RNA: DNA ratiofollowed the same trend as that of growthresponse and percent survival. Significantpositive correlation (r = 0.949 and 0.861) couldbe found with muscle RNA/DNA ratio and muscleRNA content with specific growth rate indifferent treatments. Significant differencewas found in tissue ascorbate levels betweenenriched plankton fed groups, being highest in T₃. Such live foodmediated vitamin transfer might be an effectivemeans to provide higher plane of nutrition forhigh survival and rapid growth for rohu larva.  相似文献   

Effect of organic fertilizer on plankton primary productivity in fish ponds   总被引：2，自引：0，他引：2  

Doychin Terziyski  George Grozev  Roumen Kalchev  Angelina Stoeva 《Aquaculture International》2007,15(3-4):181-190

The effect of cattle organic fertilizer on plankton primary production and related variables was tested in six Bulgarian fish ponds situated close to the town of Plovdiv in Bulgaria. At the beginning of the experiment (16th of May 2004, about 10 days after filling with water) a suppression of plankton metabolism was observed and after that the ponds treated with manure demonstrated significantly higher productivity than the controls, especially during the transition to the autumn season when the water column was more regularly mixed. The other measured variables (Secchi disk readings, plankton chlorophyll-a and respiration, assimilation number per unit chlorophyll-a) did not show significant differences between the treated and control ponds.  相似文献   

Incorporation of Maize Gluten in Supplementary Feed and its Impact on Growth and Flesh Quality of Some Carps     

V.I.?KaurEmail author  P.K.?Saxena 《Aquaculture International》2005,13(6):555-573

Maize gluten (a byproduct from the starch industry) was used at four different levels (5, 10, 15 and 20% w/w) replacing the fish meal, a component of supplementary diet, under a semi-intensive culture system. The impact of different diets on the physico-chemical parameters of water, growth and flesh quality of catla, Catla catla; rohu, Labeo rohita; and mrigal, Cirrhina mrigala was studied. Growth in terms of body weight gain was highest when fed a diet having 5% maize gluten (replacing fish meal at 25% level) for all the fish species. However, incorporation of maize gluten at higher levels led to decline in growth, since maize gluten is deficient in essential amino acids like methionine, lysine and threonine, etc. The flesh quality in terms of total protein, total lipid, total sugar, moisture and total ash was also determined at the termination of the experiment to see whether there was any significant variation with the incorporation of maize gluten or not.  相似文献   

Growth patterns of reed (Phragmites australis): the development of reed stands in carp ponds     

David Ritterbusch 《Aquaculture International》2007,15(3-4):191-199

Reed stands exhibit apparent differences of stem diameter, shoot length, density, and invasiveness. In carp ponds, five characteristic growth patterns can be distinguished. These growth patterns are ontogenetic states of a time-dependent stand development. Invasive reeds with short shoots are found in newly occupied habitats. With continuing establishment of the stand, the shoots in the inner regions become taller and fewer, a growth pattern described as central reed. Later, only a few shoots with thick stems remain. Two alternative pathways of stand development exist: steep shore reed and reed tussocks. By mowing, the directional development of reed stands can be turned into a circulation of growth patterns. The growth patterns have implications for the selection of reed stand areas for conservation purposes.  相似文献   

添加葡萄籽等植物提取物对湘云金鲫生长性能及鱼体成分的影响   总被引：1，自引：0，他引：1  

黄光中  罗世民  曾宪文 《中国饲料》2011,(23)

选用1344尾平均体重为105 g的健康湘云金鲫鱼种。随机分为4个处理,每个处理2个重复。Ⅰ组投喂基础饲料,Ⅱ、Ⅲ、Ⅳ组在基础饲料中分别添加4 m g/kg的黄酶素及10、20 g/kg葡萄籽等植物提取物。30 d后测定湘云金鲫的生长性能和鱼体成分。结果显示:Ⅲ组与Ⅰ组、Ⅱ组、Ⅳ组比较,增重率分别提高53.77%、45.43%、20.16%(P<0.05),饵料系数分别降低33.06%、31.02%、16.44%(P<0.05);鱼体蛋白质含量显著提高(P>0.05),体脂肪含量无显著变化(P>0.05)。因此,葡萄籽等植物提取物作为饲料添加剂能显著提高湘云金鲫的生长性能、降低饵料系数、改善鱼体品质,本试验条件下,其适宜添加量为10 g/kg。  相似文献