切割取样对胚胎发育的影响   总被引：2，自引：0，他引：2  

程金华  王栋  朱化彬  郝海生 《中国畜牧杂志》2006,42(19):18-20

体视显微镜下,采用徒手持金属刀片和自制的玻璃切割针分别对小鼠和牛胚胎进行切割取样,并对取样后的胚胎进行体外培养48h,其发育率分别为76.7%(46/60)和80%(16/20),两者差异不显著(P>0.05);奶牛新鲜胚胎切割取样后,胚胎移植妊娠率47.1%(8/17),比对照组妊娠率59.0%(23/39)差异显著(P<0.05)。冷冻-解冻胚胎切割取样后移植妊娠率42.9%(6/17)虽然也比对照组50%低,但是差异不显著(P>0.05)。  相似文献   

PGF2αeCG及hCG 3种生殖激素联用对母犬发情、卵泡发育及胚胎着床的影响   总被引：3，自引：0，他引：3  

谭建华  安铁洙  刘珠果  万忠海  周虚 《中国兽医学报》2002,22(4):394-396

给休情期性成熟母犬肌注 PGF2α(0 .5 m g/ kg) ,2 4 h后肌注 e CG(5 0 IU/ kg) ,再经 12 0 h后肌注 h CG(2 0 U/ kg) ,诱导其发情。将诱导发情的母犬与公犬交配 ,于交配后 16 d,摘取卵巢、子宫 ,观察其卵泡发育和胚胎着床情况。结果表明 ,药物处理可明显促使母犬提前结束休情期而进入发情期 (9/ 10 ) ,促进卵巢内卵泡发育 (P<0 .0 1) ,并使更多的胚胎着床 ,每只母犬子宫胚胎着床点为 (17.4± 2 .2 )个 ,提示 3种生殖激素联合应用可提高母犬繁殖率  相似文献   

Based serum metabolomics analysis reveals simultaneous interconnecting changes during chicken embryonic development          下载免费PDF全文

M. L. Peng  S. N. Li  Q. Q. He  J. L. Zhao  L. L. Li  H. T. Ma 《Journal of animal physiology and animal nutrition》2018,102(5):1210-1219

Metabolic disorder is a major health problem and is associated with a number of metabolic diseases. Due to native hyperglycaemia and resistance to exogenous insulin, chickens as a model had used in the studies of adipose tissue biology, metabolism and obesity. But no detailed information is available about the comprehensive changes of serum metabolites at different stages of chicken embryonic development. This study employed LC/MS‐QTOF to determine the changes of major functional metabolites at incubation day 14 (E14d), 19 (E19d) and hatching day 1 (H1d), and the associated pathways of differential metabolites during chicken embryonic development were analysed using Metabolite Set Enrichment Analysis method. Results showed that 39 metabolites were significantly changed from E14d to E19d and 68 metabolites were significantly altered from E19d to H1d in chicken embryos. Protein synthesis was promoted by increasing the concentrations of L‐glutamine and threonine, and gonadal development was promoted through increasing oestrone content from E14d to E19d in chicken embryos, which indicated that serum glutamine, threonine and oestrone contents may be considered as the candidate indicators for assessment of early embryonic development. 2‐oxoglutaric acid mainly contributed to enhancing the citric cycle, and it plays an important role in improving the growth of chicken embryos at the late development; the decreasing of L‐glutamine, L‐isoleucine and L‐leucine contents from E19d to H1d in chicken embryonic development implied their possible functions as the feed additive during early posthatch period of broiler chickens to satisfy the growth. These results provided insights into understand the roles of serum metabolites at different developmental stages of chicken embryos, it also provides available information for chicken as a model to study metabolic disease or human obesity.  相似文献   

兴仁小白壳薏苡不同播期对产量的影响     

杨连坤 《耕作与栽培》2016,(3):68-69

为探索适宜兴仁县薏苡种植的最佳播种时期，在兴仁县开展了薏苡不同播期对产量影响的试验。试验共设9个播期，梯度为7 d，播种时间从3月19日－5月14日。试验结果表明，兴仁小白壳薏苡的最佳播期是3月19日－4月16日，并且在此期间播种，薏苡产量较高。  相似文献   

不同播量拉巴豆与青贮玉米混播对草地生产性能及牧草品质的影响     

王斌  董秀  李满有  杨雨琦  兰剑 《草地学报》2021,29(4):828-834

为探究宁夏雨养区不同播量拉巴豆(Dolichos lablab)与青贮玉米(Zea mays)混播对草地生产性能和牧草品质的影响,本试验采用随机区组设计,以不同播量(0 kg·hm^-2,22.5 kg·hm^-2,45.0 kg·hm^-2,67.5 kg·hm^-2,90.0 kg·hm^-2)的拉巴豆与青贮玉米混播,并运用灰色关联度法对各处理进行了综合评价。结果表明,青贮玉米单播的鲜、干草产量分别为82.09 t·hm^-2和25.21 t·hm^-2,显著高于青贮玉米与播量为45.0 kg·hm^-2,90.0 kg·hm^-2拉巴豆混播(P＜0.05);不同播量拉巴豆与青贮玉米混播饲草的粗蛋白、粗灰分、中性和酸性洗涤纤维含量较青贮玉米单播均有不同程度的增加。经灰色关联度分析,拉巴豆播种量为22.5 kg·hm^-2与青贮玉米混播综合性状排名第一,优于其它混播处理和青贮玉米单播,可作为宁夏雨养区适宜播种量推广。  相似文献   

Effects of Donor Cells Treated with Egg Extracts on the Development Competence of Cloned Embryos     

GUO Qin-qin  FAN Zong-xing  Hao Hai-sheng  LIU Yan  ZHAO Xue-ming  ZHU Hua-bin  DU Wei-hua 《中国畜牧兽医》2016,43(2):477-486

Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research.  相似文献   

白檀未成熟胚的器官发生和植株再生     

杨艳  蒋丽娟  汤玉喜  李昌珠  唐洁  李永进 《北方园艺》2016,(5):119-123

以白檀未成熟胚为试材,以改良MS为培养基,研究了白檀未成熟胚的器官发生和植株再生.结果表明:改良MS(mMS)能够很好的诱导愈伤组织,在添加了0.2 mg/L 6-BA+0.1 mg/L NAA的培养基中,诱导率高达92.5％;胚性愈伤组织分化最佳培养基为mMS+0.25 mg/L 6-BA+0.15 mg/L NAA,分化率为72.4％;分化出的胚性愈伤组织在空白mMS培养基上继代培养15 d,76.6％的组织分化出芽,再转入1/2mMS+ 1.5％蔗糖培养基上培养20 d,芽长至1.5 cm.  相似文献   

潜土逆转旋耕刀的三维扫描及数据处理     

周鹏  陈翠英  高建民 《农机化研究》2007,(10):29-31,56

应用ATOS2系统对旋耕刀进行了三维扫描,获得了潜土逆转旋耕刀外形点云,在此基础上对其进行了反求;应用NURBS(Non-Uniform Rational B-Spline)理论,在PRO/E中进行了重构,得到了符合计算机仿真要求的数字模型,使计算机软件较精确地模拟真实刀具的潜土逆转旋耕成为可能.  相似文献   

香菇夏季不同栽培模式对产量的影响研究     

王健 《南方园艺》2013,(6):11-13

研究了香菇在夏季栽培中的露地栽培、半地下式栽培、半覆土栽培等3种不同栽培模式对产量的影响。比较表明：半覆土栽培的较有优势，生物转化率高。  相似文献   

小麦幼胚愈伤组织辐照后代的遗传变异分析     

温之雨  张艳敏  郭北海  李洪杰  王子宁 《河北农业大学学报》2000,23(1):12-15

以３个普通小麦品种为材料，用＾６０Ｃｏγ射线辐照小麦幼胚愈伤组织，在ＭＲ１、ＭＲ２代中才观察到广泛变异，其后代在育性、株高、抽穗期、千粒重、株型、蛋白质含量等方面者发生了明显变异，这些变异较单纯用组织培养得到的无性系的后代的变异频率更大，变异范围也更为广泛，并且从ＭＲ２中得到了几个有较高利用价值的变异株系。  相似文献