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The major role of DNA polymerase β was thought to be limited in its involvement in short patch base excision repair by removing 5’-deoxyribose phosphate and base insertion. However, the recent researches indicate that polymerase β might take part in a wide spectrum of DNA metabolism reactions, including long patch base excision repair, DNA replication, recombination, meiosis and transleisional DNA synthesis. Because of its wide and important cellular function, an inappropriate intracellular polymerase β level might be associated with genomic instability. Down-regulation or mutation of polymerase β is mutagenic due to deficient in DNA repair, while overexpression of this error-prone β polymerase might perturb the normal function of other accurate polymerases and cause genomic instability as well. 相似文献
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M. Heidaritabar M.P.L. Calus H‐J. Megens A. Vereijken M.A.M. Groenen J.W.M. Bastiaansen 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2016,133(3):167-179
There is an increasing interest in using whole‐genome sequence data in genomic selection breeding programmes. Prediction of breeding values is expected to be more accurate when whole‐genome sequence is used, because the causal mutations are assumed to be in the data. We performed genomic prediction for the number of eggs in white layers using imputed whole‐genome resequence data including ~4.6 million SNPs. The prediction accuracies based on sequence data were compared with the accuracies from the 60 K SNP panel. Predictions were based on genomic best linear unbiased prediction (GBLUP) as well as a Bayesian variable selection model (BayesC). Moreover, the prediction accuracy from using different types of variants (synonymous, non‐synonymous and non‐coding SNPs) was evaluated. Genomic prediction using the 60 K SNP panel resulted in a prediction accuracy of 0.74 when GBLUP was applied. With sequence data, there was a small increase (~1%) in prediction accuracy over the 60 K genotypes. With both 60 K SNP panel and sequence data, GBLUP slightly outperformed BayesC in predicting the breeding values. Selection of SNPs more likely to affect the phenotype (i.e. non‐synonymous SNPs) did not improve the accuracy of genomic prediction. The fact that sequence data were based on imputation from a small number of sequenced animals may have limited the potential to improve the prediction accuracy. A small reference population (n = 1004) and possible exclusion of many causal SNPs during quality control can be other possible reasons for limited benefit of sequence data. We expect, however, that the limited improvement is because the 60 K SNP panel was already sufficiently dense to accurately determine the relationships between animals in our data. 相似文献
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Mardassi BB Béjaoui Khiari A Oussaief L Landoulsi A Brik C Mlik B Amouna F 《Veterinary microbiology》2007,119(1):31-41
A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-amino acid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM. 相似文献
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核桃叶片基因组DNA的4种提取方法比较 总被引：2，自引：0，他引：2
以核桃叶片为材料,比较了高盐低pH法、SDS法、改良CTAB法和适合酚类、多糖类物质较多的植物DNA提取法提取基因组DNA的效果;通过琼脂糖凝胶电泳、紫外分光光度计、RAPD、ISSR等方法进行PCR扩增和用EcoRI、PstI内切酶酶切对所提取的DNA样品进行检测,比较所得DNA的产量和质量.结果表明,4种提取方法从叶片中得到的DNA产量和质量有所不同,其中酚类、多糖类物质较多的植物DNA提取法产量最高,纯度适中.4种方法提取的DNA对以后的PCR和酶切均没有影响. 相似文献
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