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An in vitro plant regeneration protocol was successfully established in Satyrium nepalense, a terrestrial orchid by culturing immature seeds from unripe fruits. Seeds were germinated on Murashige and Skoog, Knudson C and Knudson C modified Morel medium. The germination of the seeds and development of protocorm was highest in MS medium (86.7%) followed by Knudson C modified Morel medium (74%) and Knudson C medium (61.2%). Among the cytokinins tried for multiple shoot induction from the protocorm, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) was found to be superior. Indole-3-butyric acid (IBA) was effective for inducing healthy roots. Well-developed plantlets were hardened in vermicompost (leaf litter + cow dung 1:1), sand and coconut husk (1:1:1). 相似文献
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台湾金线连经组织培养诱芽微繁能形成完整植株,但生长太慢,繁殖系数太低。在诱芽培养基中添加2,4D,改用诱导类原球茎微繁,能提高繁殖系数和植株的生长速度,并能移栽成活。通过对试管苗移栽前的生根处理和移栽基质的研究,摸索出了提高移栽成活率的经验。 相似文献
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蝴蝶兰种子胚组织培养优化技术研究 总被引:4,自引:0,他引:4
以蝴蝶兰种子胚为外植体,探讨了不同采收期、不同培养基以及在培养基中添加多种附加物对原球茎形成和成苗的影响。结果表明:授粉后120~150 d的成熟种子胚适宜于组织培养,以花宝1号为基本培养基,附加椰子汁、香蕉、土豆汁能很好地诱导原球茎,诱导率达95%以上。添加有机附加物对不同花色的蝴蝶兰品种的培养效果不同,不添加植物生长调节剂也有利于种子萌发。用花多多4号3~4 g/L+Tryptone 2~2.5 g/L+1/3MS+6BA 1.0 mg/L+NAA 0.02 mg/L+CW 100 g/L+AC 0.5~1.0 g/L有利于原球体和幼苗的生长,成苗率达90%以上。 相似文献
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[目的]研究大花蕙兰组织培养与快繁技术,寻求最佳培养基。[方法]以大花蕙兰的原球茎为接种材料,以MS为基本培养基,设计不同生长调节物质浓度的组合、不同激素配比,探讨了不同培养基对大花蕙兰原球茎增殖、诱导芽及生根的影响和不同激素配比对形成根的影响。[结果]大花蕙兰组织培养原球茎增殖以MS+0.1 mg/L NAA+2.0 mg/L 6-BA培养基配方最好;适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L 6-BA+0.3 mg/L NAA,诱导出芽率可达130%;适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L 6-BA,试管苗移栽后成活率为78%。[结论]6-BA浓度的增加,可明显提高原球茎的增殖,高浓度6-BA与低浓度NAA的配比较适合大花蕙兰的原球茎增殖;6-BA在1.5 mg/L时最有利于原球茎的诱导分化。 相似文献
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[目的]对蝴蝶兰种子诱导原球茎和芽苗再生的条件进行研究。[方法]建立蝴蝶兰种子无菌播种高效萌发体系,以及原球茎诱导和芽苗再生的培养体系,对其各生长阶段的培养基以及所添加的激素组合及浓度进行选择。[结果]对种子萌发来说,选择授粉后120d未开裂的蝴蝶兰果荚内的种子播种为宜;最适培养基pH值为5.2~5.6。蝴蝶兰种子萌发最适宜的培养基为:花宝1号(300倍稀释)+3mg/L6-BA4-0.1mg/LNAA+20g/L蔗糖+2g/L蛋白胨+0.2%活性炭+6g/L琼脂;在此条件下,蝴蝶兰的萌发率最高达65%。培养基中添加活性炭能有效防止蝴蝶兰芽苗的褐化现象.添加蛋白胨能促进蝴蝶兰种子萌发和幼苗的生长。[结论]该方法研究了蝴蝶兰种子诱导原球茎和芽苗再生的培养条件,为蝴蝶兰的种质栽培提供了理论依据。 相似文献
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[目的]为优化铁皮石斛原球茎富铬培养工艺。[方法]通过Plackett-Burman试验对时间、温度、培养基铬浓度、NAA浓度、KT浓度和光照强度等因素进行考察,结果显示时间、培养基铬浓度和光照强度3个因素具有显著效应;接着,通过显著因素的最陡爬坡试验,逼近各因素最佳值区域;最后,进行响应面优化分析。[结果]最佳富铬条件为铬浓度0.37 mg/L、培养时间59 d、光照强度1 822.22 lx。原球茎铬浓度的预测值为5.08 mg/kg。[结论]响应面法能对铁皮石斛原球茎富铬培养条件进行优化分析,获得最佳工艺参数,为进一步的研究奠定基础。 相似文献
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LUO Yuan-hua LENG Qing-yun MO Rao CHEN Ye-yuan .College of Agronomy South China University of Tropical Agriculture Danzhou .Tropical Crop Genetic Resource Research Institute Chinese Academy of Tropical Agriculture Science Danzhou 《(《农业科学与技术》)编辑部》2008,(1)
[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.[Method] With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorms as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.[Result] Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as "Hyponex 1" media.The germination rate reached more than 98%.Protocorms inoculated in "Hyponex 1" media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and "Hyponex 1" media.The seedling-strengthening and rooting media were 1/2 MS media.[Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C.dayanum germplasm resource. 相似文献
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探讨适合长距石斛组织培养和快速繁殖的最佳途径。用不同激素诱导长距石斛种子形成原球茎,接入添加不同浓度激素比例的培养基中进行组织培养,研究长距石斛组织培养和快速繁殖的最佳途径。不同种类和不同浓度的激素对长距石斛原球茎分化成芽有明显影响。随2,4-D浓度的增加,长距石斛原球茎分化率增大。NAA比2,4-D更有利于长距石斛原球茎的分化。添加适合浓度6-BA有利于长距石斛苗的形成。在激素组合试验中,NAA和6-BA浓度均为1 mg/L时,发芽率达85%,叶片数多达5片。当6-BA和NAA浓度分别为2.00~3.00 mg/L和0.25~0.50 mg/L时,长距石斛原球茎的外观形态更适合于增殖。MS+2.00 mg/L 6-BA+0.50 mg/L NAA的增殖率最高。MS培养基单独添加6-BA时长距石斛种子的萌发效果较好。 相似文献