Induction of apoptosis with Salvia miltiorrhiza monomer IH764-3 via downregulating focal adhesion kinase in H₂O₂-stimulated hepatic stellate cells     

JIANG Hui-qing  ZHANG Xiao-lan  LIU Li 《园艺学报》2003,19(1):18-21

AIM: To investigate the effect of IH764-3 on the apoptosis of H₂O₂-stimulated hepatic stellate cells(HSCs) and the alteration of focal adhesion kinase (FAK). METHODS: The proliferation of HSCs was examined by direct cell count and the apoptosis was determined by Annexin-V/PI labeling, while the morphological change was observed by light microscopy and transmission electron microscopy. In addition, FAK mRNA was detected by RT-PCR. RESULTS: H₂O₂ promoted the proliferation of HSCs. IH764-3 induced the apoptosis of HSCs in a dose-dependent manner. The HSC apoptotic rates of different groups were 6. 35%,9. 28%,15. 10%,19. 69%,respectively, after treated with different concentrations of IH764-3 for 48 h while H₂O₂ group showed 2. 30%. In 30 mg/L group, the apoptosis rates were 6. 73%、10. 34%、15. 10% for the indicated time periods(12 h, 24 h, 48 h). In the presence of IH764-3, FAK mRNA decreased. The FAK mRNA reduction began at 2 h after adding IH764-3. CONCLUSION: IH764-3 induced the apoptosis of HSCs. Down-regulating the expression of FAK mRNA may be one of its mechanisms.  相似文献   

南欧丹参花提取物杀菌活性初步研究   总被引：7，自引：0，他引：7       下载免费PDF全文

麻妙锋  吴文君  张智芳  白红进 《农药学学报》2003,5(2):90-93

采用系统溶剂对南欧丹参Salvia sclarea L.花进行提取,以孢子萌发试验法、抑制菌丝生长速率法和盆栽试验系统测定了粗提物对植物病原真菌的生物活性。结果表明,石油醚为提取南欧丹参花活性成分的最佳溶剂。石油醚提取物在离体条件下能显著抑制多种供试真菌菌丝的生长,其对番茄灰霉病菌Botrytis cinerea的EC50值为77.20 μg/mL;对盆栽小麦白粉病Erysiphe graminis的防护效果为94.94%,治疗效果为84.21%。  相似文献   

Clinical,pathologic, and toxicologic characterization of Salvia reflexa (lance-leaf sage) poisoning in cattle fed contaminated hay     

Kip E. Panter  Bryan L. Stegelmeier  Dale R. Gardner  Clinton A. Stonecipher  Stephen T. Lee  Don Kitchen  Adeline Brackett  Charlie Davis 《Journal of veterinary diagnostic investigation》2021,33(3):538

Salvia reflexa (lance-leaf sage)-contaminated alfalfa hay was fed to ~500 mixed-breed beef cattle. Within hours of exposure, nearly half of the cattle developed lethargy, anorexia, depression, and recumbency, followed by bellowing, colic, and death. Even though the uneaten contaminated hay was removed the first day, nearly 100 animals died within the first 48 h. Three of these cattle were examined postmortem, and tissues and hay samples were collected for microscopic and chemical analysis. Several days later, a smaller number of the clinically poisoned cattle developed neurologic disease with aberrant behavior, aggression, icterus, blindness, exhaustion, and death. A total of 165 cattle were fatally poisoned. Poisoned cattle had swollen, dark, mottled livers that had a prominent nutmeg-like lobular pattern on cut section. Histologically, there was severe centrilobular-to-panlobular hepatic necrosis with marked hepatocellular swelling, degeneration, and necrosis. The surviving cattle developed liver disease characterized by altered serum biochemical analyses and microscopic hepatocellular degeneration and necrosis. In subsequent biopsies and analysis, these lesions resolved within 6–7 mo. After confirming toxicity of the hay in cattle, goats, and mice, followed by a mouse bioassay–guided chemical fractionation process, Salvia reflexa was identified as the contaminant in the hay responsible for the hepatotoxicity. S. reflexa has not been reported previously to cause fatal hepatotoxicity in livestock in North America, to our knowledge.  相似文献   

黄土高原芨芨草光合与蒸腾作用的初步研究   总被引：15，自引：5，他引：10  

王静  程积民  万惠娥  方锋  布都会 《草业学报》2003,12(6):47-52

对芨芨草叶片的生理生态特性进行了初步分析,结果表明,1)芨芨草叶片光合速率日进程受环境影响,在不同时期差异较大,6、7月呈双峰曲线,8月和9月,光合速率则呈单峰曲线;芨芨草蒸腾速率和气孔导度日进程在整个生育期内基本上呈单峰曲线。2)回归分析和相关分析表明,不同时期对芨芨草叶片光合速率、蒸腾速率和气孔导度起决定性作用的环境因子不同,而在整个生育期它们三者之间都存在着极显著的正相关关系。  相似文献   

盐碱、温度及其互作对丹参种子萌发及幼苗生长的影响     

郭猛  黄勇  李贺敏  张红瑞  周艳  高致明  丁梦瑶 《草业科学》2022,38(4):664-672

丹参(Salvia miltiorrhiza)是我国传统药用植物,本研究以丹参种子为试验材料,研究了两种变温(18℃/28℃和28℃/38℃)条件下,4种浓度(6.25、12.5、25、50 mmol·L-1)盐溶液和4种浓度(3.125、6.25、12.5、25 mmol·L-1)碱溶液对丹参种子萌发及幼苗生长的影响.结果表明:盐碱、温度及其互作对丹参种子萌发及幼苗生长均有显著影响(P<0.05).18℃/28℃处理下的丹参种子萌发和幼苗生长状况均显著优于28℃/38℃处理(P<0.05).在18℃/28℃条件下,6.25 mmol·L-1盐溶液对丹参种子萌发有促进作用,其发芽率和发芽指数显著高于清水对照(P<0.05),相对盐害率显著低于清水对照(P<0.05),12.5 mmol·L-1盐溶液和3.125 mmol·L-1碱溶液对丹参种子发芽率、发芽指数和相对盐害率无显著影响(P>0.05),25和50 mmol·L-1盐溶液以及6.25、12.5和25 mmol·L-1碱溶液胁迫处理均显著降低丹参种子发芽率和发芽指数(P<0.05),增加相对盐害率(P<0.05);28℃/38℃条件下,6.25和12.5 mmol·L-1盐溶液以及3.125和6.25 mmol·L-1碱溶液胁迫对丹参种子发芽率、发芽势和相对盐害率无显著影响(P>0.05),25、50 mmol·L-1盐溶液和12.5、25.0 mmol·L-1碱溶液均显著降低丹参种子发芽率和发芽指数(P<0.05),增加相对盐害率(P<0.05).盐胁迫和碱胁迫处理均显著抑制丹参苗长生长(P<0.05);两种变温条件下,6.25、12.5 mmol·L-1盐溶液和3.125 mmol·L-1碱溶液对丹参幼苗叶长生长无显著影响(P>0.05),25、50 mmol·L-1盐溶液以及6.25、12.5和25 mmol·L-1碱溶液均显著抑制叶长生长(P<0.05);18℃/28℃下6.25 mmol·L-1盐溶液对幼苗叶宽生长有促进作用,除28℃/38℃下6.25 mmol·L-1盐溶液和3.125 mmol·L-1碱溶液对叶宽生长无显著影响(P>0.05)外,其余盐碱胁迫处理均显著抑制叶宽生长(P<0.05).综上,盐碱、温度及其交互作用显著影响丹参种子萌发和幼苗生长,低温条件下盐碱对丹参种子的影响相对较小;丹参种子萌发和幼苗生长对碱胁迫更为敏感;建议丹参种子盐碱育苗时应选择盐碱度较低的土地,适宜在18℃/28℃较低温度时进行育苗.  相似文献   

烤烟、丹参轮作高效种植模式的研究     

马强  崔志军  马蒙蒙  杜金丽  张训霞  李本栋  杜玉海 《现代农业科技》2021,15(15)

本文主要通过研究烤烟-丹参轮作的种植模式,发现烟药轮作可以改善土壤结构,降低烟草土传病害的发生率和病情指数,从而增加烤烟亩产量和亩产值,增加上等烟比例,同时烟药轮作还可以提高烟叶的内在质量,促进烟草高质量发展。  相似文献   

添加配重的五杆式丹参移栽机构动力学优化设计与试验     

徐高伟  宋裕民  褚瑞霞  荐世春  邱绪云  高琦 《中国农机化学报》2022,(2):15-21

配重法能降低五杆式丹参移栽机构的振动,改善其动力学性能.为获取所添加配重的最优参数,建立五杆式丹参移栽机构的动力学模型,在动力学模型的基础上建立移栽机构的动力学优化模型,运用多目标函数获取机构不同转速下所添加配重的优化参数,通过动力学分析,确定曲柄转速为60 r/min时所对应的配重参数为最优参数组合:曲柄AB添加配重...  相似文献   

基于MaxEnt的南丹参在中国的潜在分布区预测及适生性分析     

何淑婷白碧玉  但佳惠等 《安徽农业科学》2014,(8):2311-2314

[目的]对南丹参在中国的潜在分布区进行预测,并对适生性进行分析。[方法]通过收集中草药南丹参的分布信息,利用最大熵模型(MaxEnt)与地理信息系统(ArcGIS),综合相关68项环境因子,分析南丹参在中国的分布信息。[结果]全国适合于南丹参分布的地区主要集中在湖南、江西、浙江和福建等地,其高适生区面积依次为湖南省(319.44 km2)、江西省(251.39 km2)、浙江省(122.92 km2)、福建省(62.5 km2);刀切法(Jackknife)分析显示,南丹参分布主要受到降水的影响。[结论]该研究结果为南丹参的野生资源利用及其他南丹参相关研究提供了一定技术手段和理论依据。  相似文献   

虎纹凤梨的组培快繁技术     

吴雪松  吴雪枫  甘青  龚伟  贺珑  黄逢龙 《江西林业科技》2014,(1):32-36

用虎纹凤梨母株两侧的蘖芽经消毒处理后接种到MS＋6-BA 1.0 mg/L＋IAA 0.3 mg/L的培养基中,诱导出愈伤组织。而后用改良MS培养基＋6-BA 0.3 mg/L＋NAA 0.01 mg/L从愈伤中诱导出小芽并继代培养,扩繁。增殖率3.0～4.0倍。续代3～5代后从芽团上选取H＞2.5 cm的粗壮单株仍用改良MS培养基＋6-BA 0.3 mg/L＋NAA 0.01 mg/L扩繁,增殖倍数2～3倍。而芽团上H＜2.5 cm的丛芽分成具2～3个芽的小芽团用改良MS培养基＋6-BA 0.05 mg/L＋IAA 0.01 mg/L＋C 100 mg/L使其长高长粗壮,增殖倍数1.5倍,经过一段时间的培养后切取达到生根标准的单株接到1/4 MS＋IAA 0.3 mg/L＋NAA 0.05 mg/L＋C 500 mg/L培养基中,30～40 d生根率达95%以上。炼苗20 d左右移栽,成活率达85%～90%以上。  相似文献   

Morphological responses of bedding plants to three greenhouse temperature regimes     

Richard H. Merritt  K. C. Ting 《Scientia Horticulturae》1995,60(3-4):313-324

Seedling crops of gazania, geranium, marigold, mimulus, pansy, verbena and vinca were grown to 60% anthesis in three greenhouse temperature regimes: (a) 26 °C day/6 ± 3 °C night (LT), with a 16.5 °C mean daily temperature (MDT), (b) 26 °C day/17 ± 4 °C night, with a 21 °C MDT, and (c) variable day/night temperatures in response to heating and cooling by a phase change material energy storage module (PCM) with an 8 ± 2 °C night and a 16 °C MDT. Crops bloomed from 2 to 17 days earlier in the 26 °C/17 °C greenhouse. The morphological characteristics of all cultivars of gazania, mimulus, pansy and verbena, geranium and vinca were similar regardless of greenhouse temperature regime. Marigold diploid and triploid cultivars and mimulus were morphologically more variable than the other bedding plant species. Crops grown in the PCM and LT greenhouses had virtually identical morphological characteristics and bloomed in about the same number of days. Plant height was not affected by DIF environments in the greenhouses. The PCM and LT greenhouses had a similar MDT, but the distribution of numbers of hours of mean daily temperatures was different.  相似文献