以CP23重组蛋白为抗原建立检测微小隐孢子虫抗体间接ELISA方法的研究   总被引：3，自引：0，他引：3  

何宏轩  张西臣  尹继刚  李建华  杨举 《中国兽医杂志》2003,39(1):3-5

摘要：以在E.coli高效表达的微小隐孢子虫子孢子表面抗原CP23为抗原，以辣根过氧化物酶(HRP)标记的山羊抗鼠IgG为二抗，建立了检测微小隐孢子虫抗体的间接ELISA方法。经检测筛选出最佳反应条件为1μg／孔纯化的E.coli表达的CP23抗原包被酶标板，用10％免血清进行封闭，以正常E.coli裂解上清液稀释待检血清。实验表明应用CP23重组蛋白作为诊断C.parvum抗原具有特异性高、抗原易纯化和成本低等特点。  相似文献   

Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation     

G. Tuitert  Y. Hofmeester 《European journal of plant pathology / European Foundation for Plant Pathology》1994,100(1):19-53

A field experiment was set up in 1988 to study the development of rhizomania disease of sugar beet at different inoculum levels of beet necrotic yellow vein virus (BNYVV) in soil. Five, tenfold different, inoculum levels were created by addition of the approximate amounts of 0, 0.5, 5, 50 and 500 kg infested soil per ha (the latter corresponding to 0.01% v/v calculated to the tillage layer). A drip irrigation treatment was applied to study the influence of soil moisture on disease. Susceptible sugar beet, cv. Regina, was grown for three consecutive years.In the first year, root symptoms were not observed, but BNYVV-infected plants were detected by ELISA in low numbers at all inoculum levels at harvest. After late drilling in 1989, high numbers of infected plants, up to 90–100% in plots with the highest inoculum level, were detected already in June. Root symptoms were also observed from June onwards. In both these years disease incidence increased in time and was significantly influenced by the initial inoculum level. In the third year, the whole field was heavily diseased, and only for the non-irrigated plots incidence differed for different initial inoculum levels. The expression of symptoms by BNYVV-infected plants was influenced by initial inoculum level, thus by the amount and timing of primary infection.Root weight at harvest was not affected, but sugar content decreased with increasing inoculum level already in 1988, leading to a reduction in sugar yield of 10% at the highest inoculum level. In 1989, both root weight and sugar content decreased progressively with increasing inoculum level, resulting in sugar yield reductions of 11–66% (down to approximately 3000 kg ha^–1) for low to high inoculum levels compared to the control. As the control plots became contaminated, all yields were low in 1990, still showing a decrease with increasing inoculum level in the non-irrigated plots, but an overall mean sugar yield of 3323 kg ha^–1 for the irrigated ones.Sodium and -amino nitrogen content of the root, additional quality parameters determining extractability of sucrose, showed an increase and decrease, respectively, with increasing initial inoculum level already in the first year. The relative differences in contents compared to those from the control were largest for Na content. A significant negative correlation was found between Na (mmol kg^–1 root) and sugar content (% of fresh weight); linear for 1988, exponential for 1989 and 1990.In spring 1989, the infestation of individual plots was assessed using a quantitative bioassay estimating most probable numbers (MPNs) of infective units of BNYVV per 100 g dry soil. The relationship between the MPns determined and root weight, sugar content and sugar yield at harvest could be described by Gompertz curves. The increase in disease incidence with increasing MPN in 1989 was adequately fitted with a logistic equation.  相似文献   

Molecular Identification of a New Member of the Clover Proliferation Phytoplasma Group (16SrVI) Associated with Centaurea Solstitialis Virescence in Italy     

Francesco Faggioli  Graziella Pasquini  Valentina Lumia  Gaetano Campobasso  Timothy L. Widmer  Paul C. Quimby Jr 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(4):353-360

In the United States, yellow starthistle (Centaurea solstitialis) is an annual invasive weed with Mediterranean origins. Malformed plants displaying witches' broom, fasciations, abortion of buds and flower virescence symptoms were observed in central Italy. Attempts to transmit the causal agent from the natural yellow starthistle host to periwinkle by grafting, resulted in typical symptoms of a phytoplasma, i.e. yellowing and shortening of internodes. The detection of phytoplasmas was obtained from both symptomatic yellow starthistle and periwinkle by the specific amplification of their 16S-23S rRNA genes. PCR amplification of extracted DNA from symptomatic plant samples gave a product of expected size. Asymptomatic plants did not give positive results. An amplicon obtained by direct PCR with universal primers P1/P7 was cloned and sequenced. The homology search using CLUSTALW program showed more than 99% similarity with Illinois elm yellows (ILEY) phytoplasma from Illinois (United States) and 97% with Brinjal little leaf (BLL) phytoplasma from India. Digestion of the nested-PCR products with restriction enzymes led to restriction fragment length polymorphism patterns referable to those described for phytoplasmas belonging to the clover proliferation (16S-VI) group. Since this is a previously undescribed disease, the name Centaurea solstitialis virescence has been tentatively assigned to it. This is a new phytoplasma with closest relationships to ILEY and BLL, but distinguishable from them on the basis of 16S rDNA homology, the different associated plant hosts and their geographical origin.  相似文献   

Comparison of phenolic profiles and antioxidant activities in skins and pulps of eleven grape cultivars(Vitis vinifera L.)     

Fu-xiang LI  Fu-hua LI  Ya-xuan YANG  Ran YIN  Jian MING 《农业科学学报》2019,18(5):1148-1158

Eleven grape cultivars were analysed to explore the variety differences of fresh grape phenolic profiles. The results showed that free phenolics were predominant in grape skins and pulps, and showed the higher antioxidant activities than bound. In 11 cultivars, Muscat Kyoho extracts had the highest total phenolic content in skins(10.525 mg GAE g~(–1) FW) and pulps(1.134 mg GAE g~(–1) FW), and exhibited the highest DPPH radical scavening capacity(EC_(50)=11.7 μg mL~(–1)) and oxygen radical absorbance capacity(ORAC) value(190.57 μmol TE g~(–1) FW) of free phenolic in skin. In addition, the most abundant phenolics in grape skins were found to be flavonoids such as kaempferol in Kyoho skin(541.2 μg g~(–1) FW), rutin, catechin and epicatechin in Muscat Kyoho skin(262.3, 86.3 and 70.0 μg g~(–1) FW, respectively). Furthermore, the principal component analysis showed a strong difference of phenolic profiles with the cultivars, existing forms and distributions. Pearson correlation coefficient analysis showed a significant linear correlation between total phenolic content and antioxidant activity(P0.05). Therefore, both skins and pulps were rich sources of bioactive phenolic compounds, and Muscat Kyoho was the ideal source among all samples.  相似文献   

刘歆的《周礼》学及其在两汉之际的传承谱系     

丁鼎 《湖南农业大学学报(自然科学版)》2016,(5):21-26

两汉之际是《周礼》学的奠基和发轫时期。刘歆是《周礼》学的奠基人和开拓者。约略说来刘歆对《周礼》学发展所做的贡献，主要有如下三端：首先，刘歆在与其父刘向受诏校理秘府图书时，不仅将《周官》（《周礼》）这部几乎不为世人所知的典籍著录于《七略》之中，而且还“独识”《周礼》的价值，顶住各方面的压力，不遗余力地向学术界推介这部在秘府之中新发现的典籍。其次，《周礼》一书于王莽当政时被立于学官、设博士主要应归功于刘歆的推动。复次，刘歆不仅研治《周礼》，撰著《周官传》四篇，而且还广收门徒，传授《周礼》之学。两汉之际著名经学家杜子春、郑兴、贾徽、郑众、贾逵及卫宏等人都曾直接或间接地师从刘歆学习过《周礼》。他们或是刘歆的弟子，或是刘歆的再传弟子，都出于刘歆的《周礼》学传承谱系。他们在《周礼》研究领域都做出了重要贡献，从而使《周礼》学逐步发扬光大。  相似文献   

土耳其斯坦裂体吸虫23kDa蛋白基因的序列分析及抗原表位预测     

宿文文  张莹  那璐  王文涛  常巧呈  张艳  王春仁 《黑龙江八一农垦大学学报》2014,26(4):26-29

为研究土耳其斯坦裂体吸虫23 kDa蛋白的生物学特性,以土耳其斯坦裂体吸虫cDNA为模版,利用PCR对23 kDa基因进行扩增,并将其克隆到T-easy载体后进行序列测定。利用生物信息学对其结构、抗原指数进行分析,并对其抗原表位进行预测。序列分析结果表明,该虫体的23 kDa基因长度为657 bp,A+T含量为57.38%,与曼氏血吸虫、埃及血吸虫和日本血吸虫的23 kDa基因的相似性分别为85.24%、83.71%和81.89%。蛋白二级结构分析表明,23 kDa蛋白经过4次跨膜,主要由6个α-螺旋、3个β-折叠、7个β转角和若干个无规则卷曲构成。抗原表位预测结果表明,该蛋白有3个B细胞抗原表位。综合分析土耳其斯坦裂体吸虫23 kDa蛋白是一种较好的抗原分子,是土耳其斯坦裂体吸虫疫苗的重要候选分子。  相似文献   

Hull-White利率模型仿真与债券估值     

陈勇  邓坤 《湖南农业大学学报(自然科学版)》2015,(4):12-15

由于“中”“庸”两词语义上的模糊性，其哲学含义颇为复杂。从郑玄到二程、吕祖谦，尤其是朱熹，都对“中庸”进行了解释，现代学者对《中庸》书名的释义更是多种多样。对于《中庸》的时代以及与子思的关系问题，中国历代学者以及西方的汉学家都提出了不同的观点，至今仍然莫衷一是。  相似文献   

论《中庸》之“中”与“诚”     

姜广辉  吴晋先 《湖南农业大学学报(自然科学版)》2015,(4):5-11

商周时代人们信仰上帝鬼神，春秋战国时期老子提出了以“无”为本的哲学本体论。至子思，则提出以“中”和“诚”为本的哲学本体论。子思认为，“中”和“诚”虽无形影，却具有与上帝、鬼神一样的至上性和神妙性，它已经不仅是一般学者所理解的“时中”之“中”和诚信之“诚”，它同时是“天下之大本”和“天之道”。后世儒者因而称子思善于“体道”。大凡“本体”问题，皆很难证实或证伪，但将“中”和“诚”视作“本体”，便使之进入了信仰的层面。子思在孔子创立仁学的哲学思想体系之后，又建立了“中”和“诚”的哲学思想体系，从而大大丰富和发展了儒学思想。  相似文献   

松辽黑猪METTL23基因多态性及其与繁殖性状的关联分析     

张琪  李娜  于永生  刘庆雨  张庆  高一  刘洪亮  张云鹏  张净搏  张树敏 《中国畜牧兽医》2021,48(12):4530-4536

为探究松辽黑猪METTL23基因多态性及其与繁殖性状的关联性,试验选取178头松辽黑猪母猪为研究对象,利用Sanger直接测序法查找METTL23基因外显子1~5的单核苷酸多态性(SNP),使用SPSS 19.0软件分析METTL23基因SNP位点与松辽黑猪繁殖性状(总产仔数、产活仔数、断奶仔猪数、初生重、3周龄重、断奶重、乳头数)的关联性。结果显示,仅在松辽黑猪METTL23基因外显子4发现1个SNP位点,命名为A62G,在A62G位点上检测到3种基因型,分别是AA、AG和GG。GG和G分别为优势基因型和优势等位基因,且A62G位点处于Hardy-Weinberg平衡状态(P>0.05)。该位点的遗传纯合度(Ho)为0.6516,高于遗传杂合度(He,0.3484),说明其在松辽黑猪群体中的变异较小;多态信息含量(PIC)为0.2877,属于中度多态位点(0.25<PIC<0.5),说明该遗传标记能够提供一定量的遗传信息。关联分析结果表明,GG基因型个体总产仔数、产活仔数和断奶仔猪数均显著或极显著高于AA基因型个体(P<0.05;P<0.01),总产仔数还显著高于AG基因型个体(P<0.05);AG基因型个体断奶仔猪数显著高于AA基因型个体(P<0.05);初生重、3周龄重、断奶重、乳头数各基因型间差异均不显著(P>0.05)。综上所述,METTL23基因外显子4存在多态性位点A62G,该位点与松辽黑猪产仔数存在显著关联,可作为松辽黑猪产仔数的遗传标记,用于分子育种。  相似文献   

Expression of fibroblast growth factor 23 by canine soft tissue sarcomas          下载免费PDF全文

M. R. Hardcastle  K. E. Dittmer 《Veterinary and comparative oncology》2016,14(3):306-317

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