Differential regulation on C5 expression in goose versus mammals by glucose/palmitate provides a potential protection for goose fatty liver     

Zidi Jin  Long Liu  Cheng Xu  Chunchi Yan  Shuo Li  Tuoyu Geng  Daoqing Gong 《Animal Science Journal》2021,92(1):e13672

Goose fatty liver is a specific type of nonalcoholic fatty liver that is protected from harmful effects associated with severe steatosis. Our previous findings suggest that suppression of the complement C5 may be relevant, but the mechanism is unclear. Therefore, in this study, we first verified the expression pattern of complement genes (including C5) during goose fatty liver formation and then determined the liver fat content and fatty acid composition by high-performance liquid chromatography (HPLC), followed by selecting the differential metabolites to treat HepG2, goose and mouse primary hepatocytes, aiming to explore the mechanism of C5 and inflammation suppression in goose fatty liver. The data confirmed the suppression of complement genes (including C5) in goose fatty livers. Moreover, fat content was significantly higher in fatty liver versus normal ones, with oleic acid and palmitic acid dominantly accounting for the difference. In line with this, high concentration of palmitate led to down regulation of C5 expression in goose primary hepatocytes whereas upregulation in mouse primary hepatocytes and HepG2 cells. In conclusion, regulation on C5 expression by fatty liver related factors including high level of palmitic acid may contribute to the protection of goose liver from severe hepatic steatosis.  相似文献   

佐剂对鸡补体水平影响的研究     

马本江  王君伟 《黑龙江畜牧兽医》1993,(10):1-3

６０只未免疫的伊莎褐雄鸡雏分成４组，于２４日龄开始用试管法以５０％溶血度为标准样检测补体。３１日时Ａ，Ｂ，Ｃ三组分别皮下注射司盘－８０＋白油，氢氧化铝胶和ＢＣＧ，Ｄ组对照；４６日龄时再次注射。从３１日龄至５６日龄每隔５天检测一次。结果表明，佐剂使鸡的补体水平普遍升高。于每次注射后第５天达到高峰，第１０天左右接近正常。其中ＢＣＧ影响现大，并于５１日龄时天对照组异显著（０．０１＜Ｐ＜０．０５）．  相似文献   

行列式定义间的等价性及某些性质的证明     

周恩 《河北北方学院学报(自然科学版)》2005,21(4):11-14,23

给出行列式两种定义的等价性，并且由第二种定义证明了行列式的一些性质．  相似文献   

闭式Leontief模型为可行的一个实用的判定程序     

陈神灿 《福建农林大学学报(自然科学版)》1998,(3)

设Ｔ是某闭式Ｌｅｏｎｔｉｅｆ投入产出模型的投入矩阵，如果有正向量Ｘ使得Ｘ≥ＴＸ，则称该模型为可行的．本文给出闭式Ｌｅｏｎｔｉｅｆ模型是否可行的一个实用的判定程序．  相似文献   

Depletion of serum alternative complement pathway activity in gilthead seabream caused by α-tocopherol and n-3 HUFA dietary deficiencies     

D. Montero  L. Tort  M.S. Izquierdo  L. Robaina  J.M. Vergara 《Fish physiology and biochemistry》1998,18(4):399-407

Studies on the alternative complement pathway (ACP) activity in fish have suggested the particular relevance of this pathway as a non-specific immune mechanism being its activation directly related with the availability of certain nutrients. In the present study ACP activity in gilthead seabream fed different dietary levels of a-tocopherol and n-3 HUFA deficiencies was studied under different stress conditions (overcrowding and repetitive chasing).A reduction in ACP activity was found when diets with deficiencies in -tocopherol and n-3 HUFA were assayed at any of the experimental conditions assayed. Levels of plasma cortisol were similar to those of fish after crowding stress subjected to dietary deficiencies at low stocking density, suggesting that dietary deficiencies produced a chronic elevation of cortisol. When fish were subjected to repetitive chasing, plasma cortisol from fish fed control diet showed a progressive increase whereas plasma cortisol levels from both dietary deficient groups showed a transitory peak 4 days after the beginning of the repetitive chasing. However, ACP activity levels of fish fed deficient diets remained lower than the control group and constant during chasing period. These results suggest that both a-tocopherol and n-3 HUFA nutritional deficiencies rather than stress are an important determinant of fish complement activity.  相似文献   

Immunostimulant effects of nucleotide isolated from yeast RNA on carp, Cyprinus carpio L.   总被引：2，自引：0，他引：2  

M Sakai  K Taniguchi  K Mamoto  H Ogawa  & M Tabata 《Journal of fish diseases》2001,24(8):433-438

Nucleotides from yeast RNA were evaluated for their ability to enhance the non-specific immune responses in carp, Cyprinus carpio . Oral administration of nucleotides to fish daily for 3 days resulted in enhanced responses of phagocytic and nitroblue tetrazolium (NBT) activities in kidney phagocytic cells. This activation of kidney cells was observed for at least 10 days post-treatment. The serum complement and lysozyme activities also increased in fish treated with nucleotides. Furthermore, the number of Aeromonas hydrophila in nucleotide-treated fish significantly decreased in the blood, kidney and liver after intraperitoneal injection. Thus yeast nucleotides appear to enhance non-specific immune responses in fish.  相似文献   

抗补体-ELISA及其在动物传染病诊断中的应用     

韩文瑜  郝崇  黎诚耀  王世若  刘玉斌 《中国兽医学报》1992,(3)

应用菊糖和酵母多糖试剂从豚鼠新鲜血清中提取补体C_3,制成菊糖—C_3和酵母多糖—C_3复合物后,以其分别免疫家兔,制备了抗C_3抗血清,经提纯并用HRP标记后,建立了抗补体—ELISA工作程序.通过对8种动物、18个类别的347份血清的检测表明,除抗空肠弯曲菌和獭兔军团菌阳性血清不发生阳性反应外,其余血清均与常规检测法有较好的符合率.  相似文献   

免疫亲和层析法分离鸡补体C3d蛋白研究     

马雪云  韩建秋  高淑霞 《西北农林科技大学学报(自然科学版)》2006,34(5):55-58

利用抗鸡C3 21肽抗体制备免疫亲和层析柱,对鸡补体C3d进行了分离,并采用间接酶联免疫吸附实验(间接ELISA),对洗脱液中的C3d组分进行了检测,以SDS-PAGE电泳方法对分离到的C3d进行了检测。结果表明,利用亲和柱层析能够分离得到纯度较高的C3d蛋白,为鸡血清中C3d的提取纯化提供了较为简便的方法。  相似文献   

高活性干酵母对中华鳖非特异性免疫功能和抗病力的影响   总被引：1，自引：0，他引：1  

刘宗英姚鹃  陈昌福谭斌汪成竹  王绍辉 《华中农业大学学报》2005,24(2):192-196

报道了高活性干酵母对中华鳖(Pelodiscus sinensis)幼鳖非特异性免疫功能和抗病力影响的研究结果。试验共设6个组，在各组饵料中高活性干酵母的添加量依次为每千克体重0mg(对照组)、600．0mg(试验Ⅰ组)、800．0nag(试验Ⅱ组)、1000．0mg(试验Ⅲ组)、1200．0mg(试验Ⅳ组)和1400．0mg(试验Ⅴ组)。投喂30d后，从每组中各取10只中华鳖幼鳖采血，测定了中华鳖幼鳖血液中自细胞的吞噬活性、血清中溶菌酶和补体活性；另从每组分别取30只中华鳖幼鳖用注射嗜水气单胞菌(Aeromonas hydrophila)的方法行人工感染，检测其抗病力。研究结果表明，各试验组中华鳖幼鳖血液中自细胞的吞噬活性均显著高于对照组。第Ⅰ和第Ⅱ组中华鳖幼鳖血清中溶菌酶活性与对照组之间没有显著性差异，而试验Ⅲ、Ⅳ组和Ⅴ组中华鳖幼鳖血清中溶菌酶活性均显著高于对照组。第Ⅱ、Ⅲ、Ⅳ和第Ⅴ组中华鳖幼鳖血清中补体活性与对照组之间存在显著差异，而第1组与对照组之间无显著性差异。投喂高活性干酵母能增强中华鳖幼鳖对人工感染A.hydrvphila活菌的抵抗力。研究结果证明在中华鳖幼鳖饵料中添加高活性干酵母的量以每千克体重1200．0mg左右为宜。  相似文献   

镉对鲫非特异性免疫力的影响   总被引：7，自引：2，他引：7  

丁磊  黄鹤忠  吴康  蔡春芳  吴萍 《农业环境科学学报》2004,23(1):64-66

采用毒性实验方法研究了镉对鲫非特异性免疫力的影响,确定镉诱导免疫毒性效应及其毒性参数。在试验剂量和时间范围内的结果显示,镉对鲫白细胞数量(WBC)、中性粒细胞的吞噬率(PR)和补体50%溶血活性(CH50)均有剂量-效应关系、时间-效应关系和Hormesis现象,1.25mg·kg-1注射染Cd2+胁迫时对WBC、PR和CH50有激活作用,2.5和3.75mg·kg-1注射染Cd2+胁迫时对WBC、PR和CH50有抑制作用;WBC顶点所对应的时间为染Cd2+后4d,PR顶点所对应的时间为染Cd2+后2d,CH50顶点所对应的时间为染Cd2+后1d。所以Cd2+对鲫具有明显的免疫毒性。  相似文献