宁南霉素等药剂对番茄黄化曲叶病毒病的田间防效     

陶秀娟 《安徽农业科学》2017,45(4)

[目的]评价宁南霉素、艾德拉和碧护3种药剂混合使用防治番茄黄花曲叶病毒病的效果。[方法]通过田间试验研究8%宁南霉素、艾德拉绿(花、果)与碧护组成的药剂对番茄黄化曲叶病毒病的防治效果。[结果]8%宁南霉素、艾德拉绿(花、果)与碧护组成的试验药剂对番茄黄化曲叶病毒病具有很好的田间防治效果,第3次施药后10、20、30 d防效均在82%以上,与对照药剂番茄黄化曲叶病毒灵差异达极显著水平,且其产量比清水对照增加25 747.5 kg/hm~2,保产率为103.90%。[结论]宁南霉素、艾德拉和碧护混合使用对番茄黄花曲叶病毒病具有较好的防治效果,为番茄黄花曲叶病毒病的有效防治提供了理论依据。  相似文献   

Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification,antiserum preparation,serological identification,and detection in pelargonium by ELISA     

I. Bouwen  D. Z. Maat 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):141-156

Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale Springtime Irene.The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from Saturnus, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.PLPV could be reliably detected by ELISA inP. zonale Springtime Irene and Amanda throughout the year with only a few exceptions. InPelargonium peltatum Tavira, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested.  相似文献   

A new approach to evaluate relative resistance and tolerance of tomato cultivars to begomoviruses causing the tomato yellow leaf curl disease in Spain   总被引：1，自引：0，他引：1  

L. Rubio †  J. R. Herrerob  J. Sarrió  P. Morenoa  J. Guerria 《Plant pathology》2003,52(6):763-769

A simple procedure to evaluate relative resistance and tolerance of tomato cultivars to the begomoviruses causing tomato yellow leaf curl (TYLC) disease in Spain was developed. To estimate the resistance and tolerance levels of a cultivar, several formulae were developed based on the ratio of infected plants, virus titre (estimated by tissue–print hybridization) and symptom intensity. The formulae were applied to five commercial tomato cultivars (Amoretto, Birloque, Royesta, Tovigreen and Ulises) naturally infected by TYLC viruses. The analyses showed that Ulises, Birloque and Tovigreen exhibited a moderate resistance, and Ulises was also highly tolerant. There was a positive correlation between symptom intensity and virus titre in infected plants, suggesting that the hybridization technique could also be used as an early estimator of tolerance. Finally, molecular hybridization and nucleotide sequence analyses of the begomovirus intergenic region showed that the local TYLC virus population consisted of a single species, Tomato yellow leaf curl virus (TYLCV, formerly TYLCV-Israel), with low genetic variation (nucleotide identity between isolates higher than 97%).  相似文献   

中国台湾番茄曲叶病毒侵染引起广东番茄黄化曲叶病   总被引：30，自引：0，他引：30  

何自福  虞皓  毛明杰  罗方芳  林奕韩  王穗涛 《农业生物技术学报》2007,15(1):119-123

广东汕头番茄(Lycopersicon eseulentum)上发生的黄化曲叶病毒病,田间症状表现为病株明显矮化、病叶褪绿黄化、叶小且卷曲和叶质较脆硬。对该病毒代表分离物(BS)基因组DNA-A克隆和测定结果表明,其全长为2740nt(GenBank acces-sion No.DQ237918),具有菜豆金色花叶病毒属(Begomovirus)病毒基因组典型特征,为闭合环状单链DNA,有6个ORFs,分别位于病毒链上的AV1、AV2及位于互补链上的AC1、AC2、AC3和AC4;在基因AV2与AC1之间有269nt的非编码区。BLAST结果显示,BSDNA-A与Begomovirus中来自亚洲的病毒同源性较高,而与美洲、非洲等地的相对较低;其中与中国台湾番茄曲叶病毒(Tomato leaf curl Taiwan virus,ToLCTWV)DNA-A全序列同源性最高,为97.7%,而二者的AV1、AV2、AC1、AC2、AC3和AC4基因及IR的同源性分别为98.6%、98.0%、98.0%、97.5%、96.3%、98.6%和96.6%,推导编码的6个蛋白的氨基酸序列同源性分别为97.7%、99.1%、97.5%、95.6%、91.8%和99.0%%。全序列系统进化关系树显示,BS与ToLCTWV的亲缘关系最近,并形成一个独立分支,再与广东番茄曲叶病毒G2(Tomato leaf curl Guangdong virus G2,ToLCGDV-[G2]和广东番茄曲叶病毒G3(Tomato leaf curl Guangdong virus G3,ToLCGDV-[G3]形成一个大的分支,而与其它33种Begomovirus病毒的亲缘关系均相对较远。这些结果表明,BS应是ToLCTWV的一个分离物。  相似文献   

复合侵染水茄的两种菜豆金色花叶病毒属病毒基因组结构特征分析     

赵丽玲  钟静  施章吉  李婷婷  丁铭  张仲凯 《植物保护学报》2020,47(2):355-364

为明确水茄Solanum torvum植株叶片邹缩、褪绿是否由菜豆金色花叶病毒属病毒侵染引起,从云南省西双版纳傣族自治州田间采集具有疑似感染症状的水茄植株叶片样品,应用菜豆金色花叶病毒属病毒简并引物和特异性引物进行PCR扩增、克隆和测序,通过生物信息软件分析比较其核苷酸序列特征,并对其进行系统发育分析。结果显示,从采集的疑似病叶中共克隆获得了5条菜豆金色花叶病毒属病毒DNA-A全序列和3条DNA-B全序列,经全序列分析发现,侵染水茄的2种菜豆金色花叶病毒属病毒分离物分别属于中国南瓜曲叶病毒(squash leaf curl China virus,SLCCNV)和野茼蒿黄脉病毒(Crassocephalum yellow vein virus,CraYVV)。SLCCNV水茄分离物的基因组具有典型的菜豆金色花叶病毒属病毒双组分结构特征,与来自泰国的SLCCNV分离物(AB330078)亲缘关系最近,相似性最高达到99.0%;CraYVV水茄分离物的基因组具有典型的菜豆金色花叶病毒属病毒单组分结构特征,与来自云南省景洪市的CraYVV分离物(EF165536)亲缘关系最近,相似性最高达到97.6%。表明水茄是这2种菜豆金色花叶病毒属病毒的新寄主,并首次发现双组分和单组分菜豆金色花叶病毒属病毒可复合侵染水茄。  相似文献   

甘薯卷叶病毒江苏分离物基因组全长序列测定及其外壳蛋白基因在大肠杆菌中的表达     

乔贞贞  秦艳红  乔奇  张德胜  田雨婷  高洁  张振臣 《河南农业科学》2012,41(4):86-89

为了制备甘薯卷叶病毒(SPLCV)的特异性抗体,克隆了甘薯卷叶病毒江苏分离物SPLCV(JS∶XZ∶3-2)的全长基因,并对其基因结构及分子变异情况进行分析,同时对外壳蛋白(CP)基因进行了克隆和表达。利用PCR方法扩增并克隆了SPLCV(JS∶XZ∶3-2)的全基因组,测序分析表明,SPLCV(JS∶XZ∶3-2)基因组全长为2 834bp,含有6个开放阅读框架,与已发表的SPLCV其他分离物相比,全基因组核苷酸序列相似性为82.7%～94.4%。其中,CP基因由765个核苷酸组成,共编码254个氨基酸残基。SPLCV CP基因核苷酸序列与其他分离物的相似性在89.2%～90.6%,其中与SPLCV美国分离物(HQ333135)的相似性最高,为90.6%;与日本分离物(AB433788)的相似性最低,为89.2%;与中国大陆分离物(FJ515896)CP基因的核苷酸序列相似性为90.2%。将SPLCV CP基因克隆到原核表达载体pGEX-4T-3上,获得重组质粒,经IPTG诱导表达、SDS-PAGE分析得到了大小约为54.3kD的融合蛋白条带,说明CP基因在大肠杆菌中得到了高效表达。  相似文献   

运动电磁场的Maxwell方程组-电场与磁场的关系(3)     

陈有君 《内蒙古农业大学学报(自然科学版)》2003,24(1):79-86

对运动电磁场的电场强度变量和磁感应强度变量做旋度及散度运算分析，表明除电位移变量项与经典Mexwell方程组的正负号不同外，大多数时变电磁场理论下的Maxwell方程都可以在一定条件下从运动电磁场的Maxweel方程组中导出。但在有些条件下运动电磁场的Maxwell创方程组仍然可以应用，并能得到正确的结果，而经典Maxwell方程组无法应用。这说明用“运动电场具有磁场特性；运动磁场具有电场特性”来描述电场与磁场之间的关系要比时变电磁场理论更符合自然规律。时变电磁场理论的Maxwell方程组是运动电磁场在特殊条件下的特殊形式。所谓时变电磁场可能是运动电磁场在特殊条件下的特殊表现，时变电场与时变磁场之间的相互转化可能是运动电磁场在特殊条件下所表现出的假象，并非电磁场的真正规律。时变电磁场理论的Maxwell方程组作为数学工具在一些领域中可以应用，但作为原理表达式，它们不是每个都正确的，也不是总对的。  相似文献   

Papaya yellow leaf curl virus: A newly identified begomovirus infecting Carica papaya L. from the Indian Subcontinent     

Chitra Nehra  Avinash Marwal  Rakesh Kumar Verma  Megha Mishra  Pradeep Sharma 《The Journal of Horticultural Science and Biotechnology》2019,94(4):475-480

India is one of the world’s largest producers of papaya. Viruses, mainly begomoviruses and potyviruses, cause a significant loss in papaya production. The study described here has identified a new species of begomovirus and a new species of betasatellite infecting Carica papaya in India. The sequences of the begomovirus and betasatellite show 90.03% nucleotide sequence identity to an isolate of Radish leaf curl virus and 92.25% identity to an isolate of Tomato leaf curl betasatellite, respectively. Maximum likelihood phylogenetic tree of begomovirus sequence of isolate DP2 (KX353622) showed distant relationships with previously characterised begomoviruses. Recombination analysis proposed six recombination breakpoints in begomovirus genome with other geographical begomovirus isolates.  相似文献   

一种适于提取复烤烟叶DNA的方法     

任如意  魏继承  刘瑞林 《中国林副特产》2001,(3):2-3

以改良CTAB法提取复烤烟叶DNA，经U－3000紫外分光光度计测定，所获DNA呈典型的吸收曲线，且OD260／OD280在1．7－1．9之间；对烟草叶绿体不编码序列进行PCR扩增，获得理想条带。  相似文献   

不同种质番茄材料抗番茄黄化曲叶病毒病特性研究     

王贤  刘放  魏小红  朱晓林  王宝强 《浙江农业学报》2021,33(11):2085

以不同种质番茄为实验材料,利用苗期农杆菌接种法接种番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV),采用实时定量PCR和生理生化方法评价对TYLCV的抗性,统计分析不同抗性番茄材料不同发病时期的表型特征、带毒率、抗氧化酶活性、总酚与类黄酮含量及抗性基因表达量的变化情况,并分析各抗性指标之间的关系。结果表明,番茄材料带毒率为100%;853没有任何感病症状,802与803感病最为严重;抗氧化酶活性、总酚与类黄酮含量及抗性基因表达量在各时期均较高的有867、857、853,817与842次之,803最低。SOD活性、CAT活性、APX活性、总酚含量、类黄酮含量、Ty-1基因、Ty-2和Ty-3基因的表达量相互之间均呈极显著正相关(总酚与Ty-1之间为显著正相关,P<0.01)。番茄植株感染TYLCV后,Ty-1的表达相比于其他抗病基因具有延迟性。综合分析筛选出867、857、853三种抗病性较强的材料,植株体内的抗性基因、抗氧化酶、总酚与类黄酮之间呈正相关,共同提高了番茄植株对TYLCV的抗性。  相似文献