免疫剂量和母源抗体对禽流感重组鸡痘病毒活载体疫苗免疫效力的影响   总被引：4，自引：0，他引：4  

贾立军  张艳梅  彭大新  刘红旗  程坚  张如宽  李秀梵 《中国兽医学报》2004,24(2):150-152

利用表达 H 5亚型禽流感病毒 (AIV)血凝素基因的重组鸡痘病毒 (r FPV- HA)以不同剂量免疫 1日龄 SPF鸡、有或无母源抗体 (FPV、AIV H5)的商品鸡 ,并于免疫后 2 1d利用同亚型 AIV通过肌肉注射进行致死性攻击 ,通过检测免疫后 HI抗体应答、比较攻毒后发病率和死亡率评价免疫剂量和母源抗体对 r FPV- HA免疫效力的影响。结果发现 ,免疫后 2 1d,15 %～ 2 0 %的 SPF鸡和无母源抗体商品鸡可检出 HI抗体 ,而含母源抗体商品鸡检测不到 HI抗体。利用H5亚型 AIV致死性攻击后 ,10 3～ 10 6 PFU的 r FPV- HA可保护 95 %～ 10 0 %的 SPF鸡和无母源抗体商品鸡抵御强毒攻击 ,使之免于发病和死亡 ;而不同剂量 r FPV- HA接种的含母源抗体商品鸡有 80 %～ 90 %发病和死亡。结果表明 ,在较宽的免疫剂量范围内 ,r FPV- HA对 SPF鸡和无母源抗体商品鸡可提供良好的保护 ,显示出一定的应用前景 ;母源抗体影响 r FPV- HA诱导的免疫应答 ,且提高免疫剂量亦不能克服其干扰作用 ,这提示在实际应用中需优化免疫程序 ,避免母源抗体干扰。  相似文献   

人工诱发鸡新城疫的攻毒条件的比较研究   总被引：1，自引：0，他引：1  

周秀红  祁克宗  朱良强  潘鑫 《水禽世界》2005,(8):10-12

将4周龄淮南麻黄鸡80羽随机平均分成4组：口鼻接种组、皮下接种组、肌肉接种组和空白对照组，分别用不同稀释度的NDV攻毒，观察攻毒结果并统计死亡情况。结果不同攻毒途径的致病性有差异，肌肉注射致病性最强，皮下注射次之，滴鼻滴口最弱。不同攻毒途径所需的攻毒剂量各不相同，滴鼻滴口所需的剂量最大，皮下注射次之，肌肉注射量最小。本文对造成不同攻毒途径致病性差异的原因进行了深入探讨。人工诱发新城疫，建议采用注射途径。  相似文献   

Copy number variations of MTHFSD gene across pig breeds and its association with litter size traits in Chinese indigenous Xiang pig          下载免费PDF全文

Shi‐Hui Huang  Chang Liu  Xi Niu  Sheng Li  Jia‐Fu Wang 《Journal of animal physiology and animal nutrition》2018,102(5):1320-1327

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不同用量松土促根剂对冬小麦产量的影响   总被引：1，自引：0，他引：1  

张传忠 《河南农业科学》2014,43(8)

研究了Agri-Star松土促根剂在冬小麦上的合理用量,为该产品大面积推广提供依据.结果表明,在正常旋耕施肥(配方肥750 kg/hm2+旋耕12 cm,CK2)的基础上,获得最高产量的松土促根剂施用量为18.13 kg/hm2,最佳经济施用量为16.87 kg/hm2,施用松土促根剂13.5 kg/hm2处理较CK2平均增产26.18％.施Agri-Star松土促根剂是小麦生产上值得推广的新技术.  相似文献   

The effects of straw return on potassium fertilization rate and time in the rice–wheat rotation     

Xinlin Zhao  Dianjun Lu  Xiaoqin Chen  Jianmin Zhou 《Soil Science and Plant Nutrition》2019,65(2):176-182

In order to investigate the effects of straw return on potassium (K) fertilizer application rate and time in the rice–wheat rotation, field experiments were conducted at three sites. The results showed that when the K rate was decreased to 70% of the recommended K dosage, crop yields showed no significant decrease. With K fertilization only at rice phase, crop yields showed no marked difference compared with that provided K fertilizer both at wheat and rice seasons. Though the NH₄OAc-extracted K and HNO₃-extracted K differed slightly among the treatments, the soil apparent K balance was negative without K fertilization. With crop straw fully incorporated, the recommended K dosage could be at least reduced by 30% at the experimental sites and the K fertilizer could be applied only at rice phase. A further hypothesis can be made that the best K rate was the amount of K took away by crop grain. In the long run, straw return combined with K fertilization would be an effective method to maintain soil K fertility and productivity.  相似文献   

氮肥施用对直播冬油菜产量及氮肥利用率的影响     

李敏  韩上  武际  王慧  唐杉  程文龙  石祖梁 《农业环境科学学报》2020,37(1):51-58

为探明安徽省直播冬油菜种植中适宜的氮肥用量、氮肥种类和施氮方式,通过田间小区试验,研究尿素不同用量及等氮量尿素和控释尿素不同施用方式对直播冬油菜产量、氮肥累积量及氮肥利用率的影响。结果表明：施氮水平和施氮方式对直播冬油菜产量具有显著影响,成株率与产量之间呈显著正相关,施氮量180 kg N·hm^-2条件下,尿素分次施用处理（N180）和控释尿素一次性施用处理（CRU180）在生育中后期能有效提高直播冬油菜密度。施氮量240 kg N·hm^-2、尿素分次施用处理（N240）角壳和茎秆氮素累积量分配比例增加,籽粒分配比例减少,氮肥表观利用率、氮肥农学效率、氮肥生理效率和氮肥偏生产力显著降低,籽粒产量不增反降,较CRU180和N180分别降低2.87%和9.67%。各施氮处理中,N180处理产量最高,较其他施氮处理显著增产7.53%~82.15%。施氮量180 kg N·hm^-2条件下,相比尿素一次性施用处理（U180）,CRU180和N180显著增产8.53%和16.69%,籽粒氮素累积量显著增加8.80%和16.02%,氮肥表观利用率显著增加11.68%和14.30%,氮素农学效率显著增加12.53%和24.46%,氮素生理效率增加0.74%和9.13%,氮肥偏生产力显著增加8.59%和16.76%。综合本试验直播冬油菜产量、氮素累积量和氮肥利用率结果来看,合理的氮肥用量和氮肥品种可以有效增加直播冬油菜的成株率和氮素累积量,进而增加产量、提高氮肥利用率。安徽省直播冬油菜施氮量180 kg N·hm^-2,尿素分次施用和控释尿素一次性施用,均能达到产量和氮肥利用率的双向提升。考虑到直播冬油菜轻简化发展大趋势,建议采用控释尿素一次性基施。  相似文献   

2种拟除虫菊酯对荔蝽平腹小蜂的毒力     

黄居昌  陈家骅  李金景  林久锋 《福建农林大学学报(自然科学版)》1993,(3)

40％杀灭菊酯、2.5％溴氰菊酯对荔蝽平腹小蜂成虫的LC_(30)分别为24.1×10~(-6)和35.41×10~(-6)。本试验分别取20×10~(-6)杀灭菊酯和35×10~(-6)溴氰菊酯作为亚致死剂量,对成蜂进行处理,结果表明其寿命缩短、寄生率下降。经t测验,与对照的差异极显著(1≥0.01)。用这2种农药的田间推荐使用浓度对荔蝽平腹小蜂的卵期、幼虫期、预蛹期及蛹期处理结果,最高死亡率不超过10％。因此当释放平腹小蜂的果园需要用药防治其它害虫时,可以选择使用这类农药,但要注意农药的残效作用对成蜂的影响,尽量选择平腹小蜂发育的前期喷药比较安全。  相似文献   

甲胺磷预处理小菜蛾对菜蛾绒茧蜂的影响     

孙红霞  林勇文  黄美玲  吴刚 《福建农林大学学报(自然科学版)》2007,36(5):458-461

分别在菜蛾绒茧蜂处于卵期、早期幼虫和中期幼虫时,饲喂小菜蛾2龄幼虫亚致死剂量甲胺磷,研究甲胺磷处理对寄主体内菜蛾绒茧蜂结茧率和羽化率的影响.试验表明,经亚致死剂量甲胺磷处理后,菜蛾绒茧蜂的结茧率和羽化率与对照相比均显著降低.在菜蛾绒茧蜂处于卵期时饲喂寄主幼虫,菜蛾绒茧蜂的结茧率和羽化率分别下降59.1%和32.5%;在菜蛾绒茧蜂处于早期幼虫时饲喂寄主幼虫,菜蛾绒茧蜂的结茧率和羽化率分别下降40.5%和32.8%;而在菜蛾绒茧蜂处于中期幼虫时饲喂寄主幼虫,菜蛾绒茧蜂的结茧率和羽化率分别下降18.2%和37.2%.结果表明,在绒茧蜂处于不同发育阶段时,饲喂小菜蛾幼虫甲胺磷对菜蛾绒茧蜂结茧率的影响较大,但对羽化率的影响不大.  相似文献   

Pharmacokinetics of Enrofloxacin and Its Metabolite Ciprofloxacin after Intramuscular Administration of Enrofloxacin in Goats     

Rao GS  Ramesh S  Ahmad AH  Tripathi HC  Sharma LD  Malik JK 《Veterinary research communications》2001,25(3):197-204

The pharmacokinetics of enrofloxacin and its active metabolite ciprofloxacin were investigated in goats after a single intramuscular administration of enrofloxacin at 2.5 mg/kg body weight. The plasma concentrations of enrofloxacin and ciprofloxacin were determined simultaneously by a HPLC method. The peak concentrations (C _max) of enrofloxacin (1.13 g/ml) and ciprofloxacin (0.24 g/ml) were observed at 0.8 and 1.2 h, respectively. The elimination half-life (t _1/2), volume of distribution (V _d(area)), total body clearance (Cl_B) and mean residence time (MRT) of enrofloxacin were 0.74 h, 1.42 L/kg, 1329 ml/h per kg and 1.54 h, respectively. The t _1/2, area under the plasma concentration–time curve (AUC) and the MRT of ciprofloxacin were 1.38 h, 0.74 g h/ml and 2.73 h, respectively. The metabolic conversion of enrofloxacin to ciprofloxacin was appreciable (36%) and the sum of the plasma concentrations of enrofloxacin and ciprofloxacin was maintained at or above 0.1 g/ml for up to 4 h. Enrofloxacin appears to be useful for the treatment of goat diseases associated with pathogens sensitive to this drug.  相似文献   

Pharmacokinetics and Dosage Regimen of Enrofloxacin in Buffalo Bulls after Intramuscular Administration     

Verma HK  Pangawkar GR  Chaudhary RK  Srivastava AK 《Veterinary research communications》1999,23(8):501-505

The disposition kinetics and dosage regimen of enrofloxacin were investigated in breeding buffalo bulls following a single intramuscular administration of 5 mg/kg. The absorption half-life, half-life of the terminal phase, apparent volume of distribution and total body clearance were 0.262±0.099 h, 1.97±0.23 h, 0.61±0.13 L/kg and 210.2±18.6 ml/(kg.h), respectively. Therapeutic plasma levels (1 g/ml) were maintained for up to 6 h. A satisfactory intramuscular dosage regimen for enrofloxacin in buffalo bulls would be 8.5 mg/kg followed by 8.0 mg/kg at 8 h intervals.  相似文献