Factors affecting the development of disease symptoms in potatoes infected by <Emphasis Type="Italic">Tobacco rattle virus</Emphasis>     

D.J. Robinson  M.F.B. Dale  D. Todd 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(9):921-928

Infection of potato plants with Tobacco rattle virus by its nematode vector can have different outcomes, including the development of spraing symptoms in progeny tubers. A novel syndrome described here comprises a generalized mottle in leaves on all stems, together with virtually total failure to produce daughter tubers. The outcome of infection depends partly on the potato genotype, but field trials with 15 varieties showed that the incidence and severity of spraing symptoms at three sites differed. There was no evidence that this was due to differences in virulence among the virus isolates at the sites, but it was probably the result of environmental differences that influenced the numbers and activity of the vector nematodes. At the most severely affected site, spraing symptoms were found in all varieties tested, except Record, including several that were not affected at the other two sites. Taking both severity and incidence of symptoms into account, the ranking of varieties was similar at each site. The incidence of spraing symptoms was greater in larger than in smaller tubers, and increased with later harvest dates, but did not increase when early harvested tubers were stored. Tobacco rattle viruswas detected in the roots of many of the weeds growing at one of the sites and in the roots of a few plants of the subsequent barley crop.  相似文献   

猪伪狂犬病基因缺失疫苗的制备、安全性、免疫原性、保存期测定及区域试验   总被引：3，自引：1，他引：3  

何启盖  方六荣  吴斌  刘正飞  吴美洲  肖少波  金梅林  陈焕春 《畜牧兽医学报》2005,36(10):1055-1063

为了提供有效的伪狂犬病疫苗，用鸡胚成纤维细胞扩大培养了PrV HB-98突变株（TK^-/gG^-/LacZ^＋），研制了伪狂犬病基因缺失疫苗，并对该疫苗经肌肉接种、经口等免疫途径的最小免疫剂量进行了测定，同时也对4批疫苗的安全性、效力、免疫期和保存期进行了检测；同时将4批疫苗用于免疫23个猪场的母猪、新生仔猪和育肥猪进行区域试验。测定结果表明，疫苗经上述两种途径接种对不同阶段猪的最小免疫剂量均为10^5.0 TCID50；10倍免疫剂量的疫苗对初生仔猪、15日龄仔猪和妊娠母猪是安全的，免疫猪能抵抗强毒的攻击；疫苗在4℃和-20℃下分别可保存6个月和12个月。对伪狂犬病毒抗体阴性的70日龄商品猪和种猪的免疫期为6个月。田间试验表明，4批猪伪狂犬病基因缺失疫苗安全有效，并可用于仔猪发病时的紧急接种。为猪伪狂犬病基因工程疫苗的制备与应用提供了有力的依据。  相似文献   

刺槐无性系种子园老龄母树截干矮化试验   总被引：2，自引：1，他引：1  

文彦忠 《防护林科技》2017,(5)

利用刺槐萌蘖力强的特性,对刺槐无性系种子园老龄化后的树体进行截干试验,促使其萌蘖形成新的树冠,并进行整形修剪,结果表明:老龄母树截干矮化,效果良好,达到预期目标。解决了原母树树体高大釆种困难的问题,为提高刺槐种子园老龄化后种子产量和质量进行了有益的探索。  相似文献   

Nanostructured liquid crystalline particle assisted delivery of 2,4-dichlorophenoxyacetic acid to weeds,crops and model plants     

《Crop Protection》2016

Routine agricultural practices are heavily dependent on the use of surfactants, many of which are toxic to humans and detrimental to the environment. In proof of concept work we have previously shown the potential of nanostructured liquid crystalline particles (NLCP) to safely interact with plant leaf cuticular surfaces with minimal impact on epicuticular waxes. Here we demonstrate the use of NLCP to effectively deliver the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) to plant leaves in laboratory and field studies. In the laboratory, the physiological stress responses of lupin, Lupinus angustifolius (L.) (Fabaceae) towards NLCP spray applications were shown to be much reduced in comparison with application of two common surfactants. Phytotoxicity assays of 2,4-D loaded NLCP were used to validate the herbicidal effects on Arabidopsis thaliana (L.) Heynth. (Brassicaceae) and established a similarity with that of surfactant assisted 2,4-D delivery when tested at a concentration of 0.1%. Field trials were conducted to test the efficacy of NLCP-assisted delivery of 2,4-D in comparison with commercial surfactants for the control of the invasive weed wild radish, Raphanus raphanistrum (L.) (Brassicaceae), in wheat, Triticum aestivum (L.) (Poaceae) crop fields. Compared against Estercide 800, a commercially available 2,4-D formulation, NLCP assisted delivery of 2,4-D was effective at low concentrations of 0.03% and 0.06%. The crop yield remained similar for all the tested concentrations and formulations of 2,4-D loaded NLCP and Estercide 800. This is the first report to directly show that, as an alternative to conventional methods, NLCP can be used under both laboratory and field conditions to successfully delivery an agrochemical.  相似文献   

The optimal nutrition of maize in the hungarian national long‐term field experimental network     

Tamás Kismányoky  Katalin Debreczeni 《Archives of Agronomy and Soil Science》2013,59(3-4):251-264

In Hungary, maize is grown on 1 million ha and occupies more than 20% of the arable land. The rich assortment of maize cultivars of different vegetation periods and different responses to nutritional effects, water supply etc. gives the growers the possibility to choose the cultivars suiting best the site characteristics (Jolânkai et al. 1999). Among the cereals maize has the highest genetical potential. To utilize its yield and quality potential, soil types of high nutrient content and regular nutrient supply are required (Gyõrffy, 1979). Both over‐ and under‐fertilization have an unfavourable effect on the yield and quality of maize (Debreczeni, 1985). Crops can be supplied with the appropriate nutrient amounts only with the knowledge of soil characteristics in the different agro‐ecological regions (nutrient content, water supply, soil compactness, pH, nutrient supplying capacity etc.). In Hungary, a network of long‐term field fertilization trials with uniform fertilizer treatments has been maintained at nine experimental sites representing different agro‐ecological regions of the country. This experimental network gives a basis to test the nutrient responses of our main crops and calibrate their optimal nutrient supply (Kismányoky, 1991).  相似文献   

Reinvestigating adjuvants for the wild oat herbicide,flamprop-M-isopropyl. II: Field performance     

Michael W. Murphy  Matthew L. Craven  B. Terence Grayson 《Pest management science》1995,43(2):157-162

A field trials programme was conducted in which the performance of a new emulsifiable concentrate formulation (ECI) of flamprop-M-isopropyl containing the adjuvant, ‘Dobanol’ 25-7, in a ratio of 2:1 (by weight) with the AI, was compared with the current commercial formulation of ‘Commando’, in combination with its recommended adjuvant, ‘Swirl’, for the control of wild oat (Avena fatua L.) in wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.). A further treatment, in which the ‘Dobanol’ 25-7: AI ratio was increased to 4:1 by the spray tank addition of the former, was also included. The mean results from six trials (five wheat, one barley) showed that the addition of ‘Swirl’ to ‘Commando’ was beneficial, increasing wild oat floret control from a mean value of 80% to 92% at current recommended rates (flamprop-M-isopropyl, 600 g ha^?1; ‘Swirl’, 2.5 litre ha^?1). However, combinations of flamprop-M-isopropyl and ‘Dobanol’ 25-7 gave superior levels of control even at lower AI application rates. For example, a mean level of 96% control of Avena spp. was obtained at 300 g AI ha^?1 with 1200 g ha^?1 ‘Dobanol’ 25–7; with even better control at higher rates of application of both components. This improvement in performance was accompanied by a higher risk of crop phytotoxicity than observed with the ‘Commando’/‘Swirl’ mixtures. Symptoms initially were scorch and subsequently growth depression, particularly of tillers. None of the mean values in the six ‘efficacy’ trials reached commercially unacceptable levels, but in a further six ‘crop effects’ trials (three wheat, three barley), in which double rates were applied, the levels of phytotoxicity did become unacceptable and subsequently reduced grain yields. In contrast, two barley ‘crop effects’ trials gave yields higher than the control plots, possibly through the effects of reducing stem length and lodging thereby enabling more efficient harvesting. Nevertheless, there were rates of application of flamprop-M-isopropyl in the range 300–400 g ha^?1 with ratios of ‘Dobanol’ 25-7 in the range 2:1 to 4:1 that would achieve high levels of control of Avena spp. without undue risk of crop phytotoxicity and further trials are planned to support this new adjuvant system.  相似文献   

Clinical trials in spontaneous disease in dogs: a new paradigm for investigations of sepsis     

Cynthia M. Otto  DVM  PhD  DACVECC 《Journal of Veterinary Emergency and Critical Care》2007,17(4):359-367

Objective: To provide evidence that naturally occurring sepsis in dogs provides a unique opportunity to test new therapies in clinically relevant settings. Data sources: Human and veterinary literature. Human data synthesis: Sepsis is a devastating condition responsible for most intensive care unit deaths. Most clinical trials targeting inflammatory mediators of sepsis have failed to improve outcome in clinical patients despite promising results in laboratory animal models. Animal models of sepsis fail to reproduce the clinical syndrome and therefore lead to conclusions that may not be relevant to clinical care. Veterinary data synthesis: Sepsis is recognized but not well‐characterized in companion animal species. Despite some species variability, the cardiopulmonary response to sepsis in dogs is similar to humans. Additionally, inflammatory and coagulation changes that accompany canine sepsis are consistent with those documented in humans. Sepsis secondary to canine parvoviral infection offers the advantages of relative population homogeneity, predictable course, and easy early diagnosis. The disadvantages of canine parvovirus are that it affects a predominantly young and healthy population and results in low mortality with aggressive supportive care. Septic peritonitis and pneumonia have high mortality but can be challenging to diagnose, have a variable course, and affect a heterogeneous population, which can be an advantage or a disadvantage. Conclusions: Similar to trials currently being performed in canine cancer patients, veterinary clinical trials of new sepsis therapeutics may provide a unique opportunity to advance the treatment of sepsis in dogs, humans, and other species. Spontaneous sepsis from canine parvovirus, peritonitis, and pneumonia are common clinical conditions in which therapeutics can be tested.  相似文献   

爬竹雄蕊发育异常的初步观察   总被引：1，自引：0，他引：1  

庞延军  喻富根 《竹子研究汇刊》1994,13(4):42-46

本文报道了爬竹雄蕊发育中有异于正常雄蕊发育过程的现象。观察结果表明,爬竹的某些雄蕊在发育过程中,在不同的时期发生偏离于正常途径的发育,这些异常现象最早出现在雄蕊原基发育时期。异常发育的雄蕊或者不能形成花粉囊;或者形成了花粉囊但囊内细胞高度液泡化,缺乏原生质,无法形成有效的小孢子母细胞。  相似文献   

Climatology of Acacia mearnsii. 1. Characteristics of natural sites and exotic plantations     

Trevor H. Booth  Tom Jovanovic 《New Forests》1988,2(1):17-30

Climatic variation in the natural distribution of Acacia mearnsii in Australia was analysed. Data from sites where the species has been successfully grown in plantations and trials in Africa, Asia and South America were used to determine the species' climatic adaptability. Annual mean temperatures at these plantations and trial sites ranged from 13.9 to 23.9°C, whilst annual mean precipitation ranged from 693 to 2263 mm. The coldest month minimum temperature at these sites was –0.6°C. This information, along with other details of climatic requirements presented in the paper, will assist in selecting sites for future trials at new locations.  相似文献   

桉树采穗母株和采穗年限的选择     

刘斯通  黄永芳  张浩  余正国 《中南林业科技大学学报(自然科学版)》2006,26(2):79-82

桉树丰产林能否获得速生、高产,除了气候、温度和土壤等立地条件和造林措施以及种苗品种等因子外,苗木扦插繁育技术也是重要因素,其中采穗母株和采穗年限的选择是关键环节.近几年,对湛江市桉树林进行了测定和调查.结果表明:与扦插苗作母株相比,组培苗作母株能扦插的穗条产量增产100%~216.6%,组培苗造林后年平均生长量增加28.5%;组培苗的苗龄超过3 a后,穗条产量和丰产林的年平均生长量均明显下降,采穗母株的采穗年限应控制在3 a以内,3 a后必须更新.  相似文献