Effects of Adding Mannan Oligosaccharides to Diets with Different Concentrate to Forage Ratios on Ruminal Fermentation of Sheep in vitro     

ZENG Yan-xia  CHEN Zhi-long  WANG Lin  ZHAO Chen  ZHENG Chen 《中国畜牧兽医》2016,43(1):68-75

The test was aimed at determining the effects of adding MOS to diets with different concentrate to forage ratios on ruminal fermentation of sheep in vitro.The 4×6 two-factor experimental design was chosen.There were 4 diets with different concentrate to forage ratios(20:80, 30:70, 40:60 and 50:50) and added to 6 dosages MOS(0, 0.4%, 0.8%, 1.2%, 1.6% and 2.0%), respectively.The method of gas production in vitro was used to measure the total gas and CH₄ production, IVDMD, IVCPD and IVOMD after cultured for 3, 6, 9, 12 and 24 h.The results showed that the gas(except 12 and 24 h) and CH₄ production(except 9 h) were not impacted by the two factors(P>0.05).The IVDMD, IVCPD and IVOMD were significantly affected by concentrate to forage ratios(P<0.05), while the IVCPD at 24 h was significantly affected by MOS(P<0.05).Meanwhile, all the indexes were not influenced by the interaction of two factors(P>0.05).The IVDMD and IVOMD increased with the rising of concentrate approximately, while the IVCPD decreased with the rising of MOS roughly.The biggest associative effect was gained in 30:70 concentrate to forage diet when adding 1.2% MOS.  相似文献   

低聚果糖与低聚半乳糖在婴幼儿配方乳粉中的精准化应用     

王炜宏  杨新球 《中国乳业》2021,(7):102-108

母乳是婴幼儿营养配方的金标准,而低聚糖是母乳重要的活性成分。我国母乳喂养婴幼儿平均每天摄入3.5~6.4 g母乳低聚糖,而婴幼儿配方乳粉（以下简称“婴配粉”）喂养婴幼儿平均每天摄入的低聚果糖与低聚半乳糖仅0.6~3.0 g。为精准模拟母乳低聚糖的作用,低聚果糖与低聚半乳糖的含量至关重要。因此,低聚果糖与低聚半乳糖在婴配粉中的添加量有必要适当提高。  相似文献   

A Novel Alginate Lyase: Identification,Characterization, and Potential Application in Alginate Trisaccharide Preparation     

Zhao Xue  Xiao-Meng Sun  Cui Chen  Xi-Ying Zhang  Xiu-Lan Chen  Yu-Zhong Zhang  Shou-Jin Fan  Fei Xu 《Marine drugs》2022,20(3)

Alginate oligosaccharides (AOS) have many biological activities and significant applications in prebiotics, nutritional supplements, and plant growth development. Alginate lyases have unique advantages in the preparation of AOS. However, only a limited number of alginate lyases have been so far reported to have potentials in the preparation of AOS with specific degrees of polymerization. Here, an alginate-degrading strain Pseudoalteromonas arctica M9 was isolated from Sargassum, and five alginate lyases were predicted in its genome. These putative alginate lyases were expressed and their degradation products towards sodium alginate were analyzed. Among them, AlyM2 mainly generated trisaccharides, which accounted for 79.9% in the products. AlyM2 is a PL6 lyase with low sequence identity (≤28.3%) to the characterized alginate lyases and may adopt a distinct catalytic mechanism from the other PL6 alginate lyases based on sequence alignment. AlyM2 is a bifunctional endotype lyase, exhibiting the highest activity at 30 °C, pH 8.0, and 0.5 M NaCl. AlyM2 predominantly produces trisaccharides from homopolymeric M block (PM), homopolymeric G block (PG), or sodium alginate, with a trisaccharide production of 588.4 mg/g from sodium alginate, indicating its promising potential in preparing trisaccharides from these polysaccharides.  相似文献   

植物低聚糖提取和生物活性鉴定   总被引：1，自引：0，他引：1  

李宏潮  王虹 《华北农学报》1992,7(1):60-64

应用酶解法从小麦细胞壁中提取出低聚糖。经过活性鉴定,具有诱导大豆和小麦抗毒素产生,特别是从小麦代谢、细胞、植株等水平上改善该作物体内防御功能等生物作用。水解酶的种类、纯度、酶活值与所降解低聚糖的活性程度密切相关;高纯度、高酶活单位的水解酶提取的低聚糖,具有较强的生物功能。对来源不同的低聚糖进行了活性差异的比较,同时还讨论了其它因素——诸如小麦细胞的生理状态,低聚糖保存条件等对低聚糖活性的影响。  相似文献   

响应面法优化超声波辅助提取薏苡仁低聚糖工艺的研究     

薛雅茹  操然  卢旭  张怡 《热带作物学报》2017,(3):565-571

采用响应面法优化超声波辅助提取薏苡仁低聚糖的工艺条件。在单因素试验基础上,选取液料比、超声波时间以及超声波功率3个因素结合Box-Behnken试验建立数学模型,分析考察3个因素对薏苡仁低聚糖响应值的影响程度,优化工艺参数。各因素对薏苡仁低聚糖提取率影响程度从大到小顺序依次为:超声波功率超声波时间液料比。响应面设计法优化出其最佳超声波提取条件为:超声波温度70℃,液料比33∶1(m L/g),超声波时间27 min,超声波功率450 W。在该条件下,薏苡仁低聚糖提取率为0.94%,与模型预测值0.98%接近。说明使用响应面法优化超声波辅助提取薏苡仁低聚糖的工艺条件是可行的。  相似文献   

电渗析技术在大豆低聚糖溶液脱盐上的应用   总被引：3，自引：0，他引：3  

王秋霜  应铁进  赵超艺  陈栋  卓敏  孙世利  晏嫦妤  凌彩金  潘顺顺  唐劲池  唐颢  黄国滋  Muhammad Muzammil Jahangir 《农业工程学报》2008,24(10):243-247

从甜浆中提取的大豆低聚糖粗提液中含有很多的盐类,影响糖液的纯度。该试验通过离子交换膜辅助的电渗析法对大豆低聚糖模拟溶液进行脱盐处理,确定了较好的试验操作参数。试验结果表明:电渗析法对大豆低聚糖模拟液进行脱盐是可行的,且脱盐效果较好,脱盐率达到96.07%;该试验条件下较佳的工作电压为20 V,流量为60 L/h,样品稀释倍数为15倍。通过以上条件处理后,大豆低聚糖的保留率达到83.82%。  相似文献   

代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响     

郑琛  李发弟  李飞  周巨旺  段鹏伟  刘绘汇  樊海苗  朱威力  刘婷 《中国农业科学》2020,53(2):398-408

【目的】探讨代乳粉中添加甘露寡糖（mannan oligosaccharides,MOS）对7—28日龄湖羊羔羊胃肠道生长发育的影响。【方法】选择同质性良好的7日龄湖羊公羔（双羔）30只,随机分为2组,每组15只,每只为1个重复,对照组羔羊饲喂不含MOS的代乳粉,试验组羔羊饲喂含0.2 % MOS的代乳粉,试验期21d。羔羊28日龄时,两个试验组各随机选择8只羔羊屠宰,取出消化道,称量各胃室和肠段包含内容物的质量和净质量,量取各肠段长度,用以计算各部位的相对质量和内容物分布,以及各肠段的相对长度。多聚甲醛固定皱胃胃底腺区及十二指肠、空肠和回肠中段的组织样品,测定组织形态和小肠上皮细胞凋亡率。采集十二指肠、空肠和回肠的黏膜样品,测定紧密连接蛋白1 (claudin 1)、闭锁小带1（zonula occludens-1,ZO-1）和闭锁蛋白（occludin）的mRNA表达量。【结果】除空肠相对长度外（%全肠长度,P=0.040）,MOS对羔羊胃肠指数（%活体质量）、胃肠相对质量（%全胃质量、%全肠质量和%全胃肠质量）、肠道相对长度（%全肠长度）、内容物相对活体质量（%活体质量）、胃肠内容物相对总胃/肠内容物及总胃肠内容物相对质量（%总胃内容物、总肠内容物、总胃肠内容物）、小肠上皮细胞凋亡率和小肠黏膜claudin 1蛋白mRNA的表达量均没有产生显著影响（P＞0.05）,但MOS显著提高羔羊十二指肠绒毛高度和肌层厚度并显著降低绒毛宽度（P=0.033,P=0.047,P=0.015）,显著上调空肠ZO-1蛋白mRNA表达量（P=0.028）,此外,MOS有提高羔羊回肠绒毛高度、绒毛宽度和隐窝深度、皱胃肌层厚度及回肠occludin蛋白mRNA表达量的趋势（P=0.075,P=0.078,P=0.085,P=0.084,P=0.052）。【结论】MOS对7—28日龄湖羊羔羊胃肠道相对质量、长度和内容物分布基本无显著影响,但可改善十二指肠和回肠绒毛及肌层的组织形态,维持小肠屏障功能,有利于提高养分消化率。  相似文献   

Effects of the prebiotic mannan‐oligosaccharide on feed deprived zebrafish: Growth and reproduction          下载免费PDF全文

Mohammad Navid Forsatkar  Mohammad Ali Nematollahi  Gholamreza Rafiee  Hamid Farahmand  Christian Lawrence 《Aquaculture Research》2018,49(8):2822-2832

This study was designed to investigate whether the prebiotic mannan‐oligosaccharides (MOS) can reduce the deleterious impacts of feed deprivation on growth and reproduction in zebrafish. In the growth performance experiment, juvenile fish were distributed in the following four treatments: normal‐control (NC), starved‐control (SC), normal‐prebiotic (NP) and starved‐prebiotic (SP). After 8 weeks, NP and SC fish showed the highest and lowest growth patterns, respectively among treatments. Standard length, specific growth rate and feed conversion ratio did not differ significantly between NC and SP treatments. Autochthonous lactic acid bacteria (LAB) were significantly higher in NP and SP than the NC and SP treatments. Feed restriction resulted in significantly lower concentrations of thyroxine. In the reproductive performance experiment, additional juveniles were fed as in the growth experiment. Crossing in the final week of the experiment resulted in successful spawning in only NP fish, which showed mature sperm and oocytes in histological examinations. The number of spermatozoa was significantly lower in the fish that experienced feed restriction; however, the oocytes of SP females were at the same maturation level to that observed in NC females. Sex‐steroids changed after both starvation and MOS supplementation where NC and SP showed no differences in the levels of testosterone and female's 17β‐estradiol. These results indicate that MOS supplemented diet reduced some side effects of feed deprivation (final weight and length, SGR, FCR and levels of LAB and T3), and suggest that supplementation of the diet with MOS may ameliorate some of the negative effects of feed deprivation in zebrafish.  相似文献   

不同水平大豆寡糖(SBOS)对生长绵羊瘤胃发酵的影响     

刘光斌  刘兵  张学峰  瞿明仁  高民  张爱忠  卢德勋 《江西农业大学学报》2007,29(6):871-875

采用体外批次培养法,研究在日粮中添加不同水平SBOS对绵羊瘤胃发酵功能的影响。SBOS的添加水平分别为基础日粮的0,0.4%,0.8%,1.2%,1.6%和2.0%等6个水平,即1个对照组(0水平组)5个试验组,每个组3个重复。结果表明:体外培养24 h,日粮中添加SBOS可以增加瘤胃液NH3-N、丙酸及总VFA、MCP浓度,提高培养底物NDF降解率,降低瘤胃液pH值、乙/丙比值,对瘤胃产气量、瘤胃液乙酸、丁酸浓度影响不显著。应用综合评定指标(MFAEI)对产气量、pH、MCP浓度、总VFA浓度、NH3-N浓度和NDF含量进行综合评定,得出添加量为1.2%的SBOS更有利于调控瘤胃发酵。  相似文献   

功能性低聚糖在饲料工业上的应用研究进展     

徐骏  高峰  周光宏 《家畜生态》2006,(6)

功能性低聚糖是一种新型、绿色饲料添加剂,它能够增殖有益菌、抑制病原菌,从而调节肠道微生态系统;可被有益菌利用产生短链脂肪酸、维生素等,为动物提供营养;同时也能够改善脂类代谢和促进日粮中一些矿物质的吸收。本文对功能性低聚糖的种类、应用、作用机理、影响应用效果的因素、应用过程中存在的问题及推广应用前景进行了全面论述。  相似文献