Induction of Drought Tolerance in Maize (Zea mays L.) due to Exogenous Application of Trehalose: Growth,Photosynthesis, Water Relations and Oxidative Defence Mechanism     

Q. Ali  M. Ashraf 《Journal of Agronomy and Crop Science》2011,197(4):258-271

The present investigation was conducted to assess the ameliorative effects of foliar‐applied trehalose on growth, photosynthetic attributes, water relation parameters and oxidative defence mechanism in two maize cultivars under field water deficit conditions. Various components of the experiment comprised two maize cultivars (EV‐1098 and Agaiti‐2002), two water‐stress levels (irrigation after 2 weeks and irrigation after 3 weeks during the entire period of growth), and two levels of trehalose (0 and 30 mm ) and four replicates of each treatment. Water stress significantly reduced the plant biomass production, photosynthetic attributes and water relation parameters in both maize cultivars. In contrast, water stress considerably increased the leaf malondialdehyde (MDA) contents, the activities of antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), and the levels of non‐enzymatic compounds such as ascorbic acid and tocopherols. In contrast, water stress caused a marked reduction in leaf phenolic contents. Foliar‐applied trehalose significantly increased plant biomass production, and improved some key photosynthetic attributes and plant–water relation parameters. The ameliorative effect of exogenously applied trehalose was also observed on the activities of some key antioxidant enzymes (POD and CAT) and non‐enzymatic compounds (tocopherols and phenolics). Overall, exogenously applied trehalose considerably improved drought tolerance of maize plants by up‐regulating photosynthetic and water relation attributes as well as antioxidant defence mechanism.  相似文献   

外源海藻糖对NaHCO3胁迫下甘草幼苗生长调节及总黄酮含量的影响     

柳福智  张迎芳  陈垣 《草业学报》2021,30(7):148-156

本研究以甘草无菌苗为试验材料,采用植物组织培养的方法,分析50 mmol·L^-1 NaHCO₃ 胁迫下外源海藻糖对甘草幼苗生长量、叶绿素含量、渗透调节物质含量、抗氧化保护酶活性和总黄酮含量的影响。结果表明： 50 mmol·L^-1 NaHCO₃ 胁迫显著降低了甘草幼苗的生长量、叶绿体色素含量、K⁺ 浓度、抗氧化保护酶（SOD、POD、CAT）活性和总黄酮含量,显著提高了丙二醛、脯氨酸和可溶性糖含量以及Na⁺浓度;施加15 mmol·L^-1 海藻糖可显著提高甘草幼苗的生长量,提高叶绿素含量、K⁺ 浓度和总黄酮含量,降低丙二酸含量、脯氨酸含量、可溶性糖含量和Na⁺ 浓度,并且提高抗氧化保护酶活性。因此,NaHCO₃胁迫下施加外源海藻糖对甘草幼苗生长具有良好的调节作用,可以增强甘草的抗碱能力,促进甘草幼苗生长。本研究为外源海藻糖提高甘草耐碱性和揭示其调控机制提供理论依据。  相似文献   

稀释液中添加海藻糖对猪冷冻精液质量参数和活性氧的影响   总被引：8，自引：2，他引：8  

金一  陈宠霞  方南洙  金英海 《中国畜牧杂志》2005,41(10):6-8

本试验旨在探讨海藻糖对猪精子冷冻效果以及精子内活性氧(ROS)的含量影响。试验分对照组和4个海藻糖处理组(0.025,0.05,0.1和0.2 mol/L)。精子冷却后,添加0.1 mol/L海藻糖处理组精子活力显著(P<0.05)高于对照组,而精子内和稀释液中各组间ROS发生量都没有显著的差异。精子冷冻解冻后,添加0.05 mol/L海藻糖处理组的精子活力显著(P<0.05)高于对照组;添加0.05 mol/L和0.1 mol/L海藻糖处理组的精子生存率显著(P<0.05)高于对照组;添加0.1 mol/L海藻糖处理组顶体完整的精子百分比显著(P<0.05)高于对照组;4个海藻糖处理组膨胀精子百分比都显著(P<0.05)高于对照组;添加0.025 mol/L海藻糖处理组ROS水平显著(P<0.05)低于对照组。结果表明:海藻糖对精子冷冻保存是有益的,且能抑制精子内ROS的发生,但它的作用机制有待于进一步的研究。  相似文献   

海藻糖对冻藏过程中鳙肌原纤维蛋白冷冻变性的影响   总被引：10，自引：0，他引：10       下载免费PDF全文

薛勇  薛长湖  李兆杰  刘艺杰  王进勉 《中国水产科学》2006,13(4):637-641

从鳙（Aristicktkys nobilis）肌肉中提取肌原纤维蛋白，在蛋白溶液中加入10％（W／V）蔗糖、山梨醇混合物（质量比1：1）或10％（W／V）海藻糖。蛋白溶液在-18℃下冻藏30d，测定冻藏过程中肌原纤维蛋白盐溶性、Ca^2＋-ATPase活性、活性巯基含量、总巯基含量、表面疏水性变化和冻藏结束时的SDS-PAGE。测定结果表明，蔗糖、山梨醇混合物和海藻糖都抑制了冻藏过程中肌原纤维蛋白盐溶性、Ca^2＋-ATPase活性、巯基含量的降低和表面疏水性的升高，延缓了鳙肌原纤维蛋白的冷冻变性。海藻糖比蔗糖、山梨醇混合物有更好的抗冷冻变性效果。[中国水产科学，2006，13（4）：637—641]  相似文献   

Prd29A启动子在小麦中的诱导表达活性及大肠杆菌海藻糖合成酶基因的转化   总被引：1，自引：0，他引：1  

康旭升  刘立科  刘根齐  侯宁  戴秀玉  刘春光 《农业生物技术学报》2007,15(3):434-438

以gus基因为报告基因，通过瞬时表达测定了Prd29A诱导型启动子在小麦愈伤组织中的诱导表达活性。结果表明，Prd29A-gus基因的表达水平可在不同浓度NaCl盐的胁迫条件下得到显著提高。由此证实，Prd29A启动子在小麦中具有较强的诱导表达活性。在此基础上，将Prd29A诱导型启动子驱动下的大肠杆菌海藻糖合成酶基因（otsA）采用花粉管途径导入目的小麦品系，经PCR筛选、Southern鉴定及转化后代海藻糖含量的测定，获得了一批otsA转基因植株及株系，为进一步选育耐盐抗旱的转基因小麦新品系奠定了基础．  相似文献   

海藻糖对蛋糕品质影响的研究     

陈佩  赵冰  庞宇辰 《广东农业科学》2015,42(3):94-98

探究了海藻糖对蛋糕品质的影响,以期开发出集品质、健康营养于一身的新型蛋糕。在传统蛋糕的基础上,用海藻糖替代传统蛋糕中30%~50%的蔗糖,以成品蛋糕的比容、水分活度、失水率、质构性质(硬度和咀嚼性、弹性和回复性、粘聚性)和感官评定为指标,研究其对蛋糕品质的影响。结果表明:当取代量为35%时,蛋糕比容最大,在30%~50%的取代范围内,随着海藻糖取代量的增加,蛋糕的水分活度明显降低,持水性增加,感官品质得到改善,用主成分分析法得出海藻糖的建议添加量为30%。  相似文献   

外源海藻糖对家蚕生长和茧丝品质的影响     

肖丽蓉  张友洪  周安莲  肖文福  肖金树  杨文娟  李方方 《安徽农业科学》2012,40(3):1487-1489

[目的]探讨外源海藻糖对家蚕的生长和茧丝品质的影响。[方法]通过对家蚕品种菁松×皓月进行添食试验,研究添加海藻糖对家蚕的生长和茧丝品质的影响。[结果]添加海藻糖后家蚕上蔟更为整齐,茧丝纤度降低,而生存率、体重、4龄结茧率、普茧率、解舒率、粒茧丝长和解舒丝长都增加。综合评价结果表明,添加20 mmol/L海藻糖的家蚕成绩最优。[结论]外源性海藻糖对提高家蚕生长和茧丝品质具有积极作用。  相似文献   

外源海藻糖调节西瓜细胞渗透胁迫抗性的研究   总被引：1，自引：0，他引：1  

孙梦利  王世豪  徐子健  江雪飞  孙化鹏  乔飞  丛汉卿  于平 《热带作物学报》2019,40(2):269-274

为探究外源海藻糖对渗透胁迫下西瓜细胞生长的影响,本研究以西瓜悬浮培养细胞为试材,测定外源海藻糖预处理后,渗透胁迫对西瓜悬浮培养细胞生长量、细胞外pH、细胞内ROS（活性氧簇）相对含量和微管骨架的影响。结果表明：渗透胁迫下西瓜细胞的生长量明显受到抑制,并且渗透胁迫可诱导西瓜悬浮培养细胞质外体碱化,ROS迸发,细胞微管骨架发生解聚;外源添加海藻糖可在一定程度上缓解渗透胁迫对西瓜悬浮细胞生长量的抑制作用,调节pH、ROS表达水平、并维持微管骨架结构的完整性。以上研究结果表明渗透胁迫下,海藻糖对西瓜细胞具有维持细胞生长、保护亚细胞结构并调节抗性反应的功能。  相似文献   

Effect of trehalose supplementation in milk replacer on the incidence of diarrhea and fecal microbiota in preweaned calves     

Hiroto Miura  Kazuhisa Mukai  Keigo Sudo  Satoshi Haga  Yutaka Suzuki  Yasuo Kobayashi  Satoshi Koike 《Journal of animal science》2021,99(1)

Trehalose, a nonreducing disaccharide consisting of d-glucose with α,α-1,1 linkage, was evaluated as a functional material to improve the gut environment in preweaned calves. In experiment 1, 173 calves were divided into two groups; the trehalose group was fed trehalose at 30 g/animal/d with milk replacer during the suckling period, and the control group was fed nonsupplemented milk replacer. Medication frequency was lower in the trehalose group (P < 0.05). In experiment 2, calves (n = 20) were divided into two groups (control group [n = 10] and trehalose group [n = 10]) based on their body weight and reared under the same feeding regimens as in experiment 1. Fresh feces were collected from individual animals at the beginning of the trial (average age 11 d), 3 wk after trehalose feeding (experimental day 22), and 1 d before weaning, and the fecal score was recorded daily. Fecal samples were analyzed for fermentation parameters and microbiota. The fecal score was significantly lower in the trehalose group than in the control group in the early stage (at an age of 14 to 18 d; P < 0.05) of the suckling period. Calves fed trehalose tended to have a higher proportion of fecal butyrate on day 22 than calves in the control group (P = 0.08). Population sizes of Clostridium spp. were significantly lower (P = 0.036), whereas those of Dialister spp. and Eubacterium spp. tended to be higher in the feces of calves in the trehalose group on day 22 (P = 0.060 and P = 0.083). These observations indicate that trehalose feeding modulated the gut environment and partially contributed to the reduction in medication frequency observed in experiment 1.  相似文献   

谷氨酸棒杆菌麦芽寡糖基海藻糖合成酶基因的克隆与表达   总被引：1，自引：1，他引：0  

张文德  乔宇  丁宏标 《中国农业科技导报》2009,11(1):68-72

采用PCR方法从谷氨酸棒杆菌（Corynebacterium glutamicum）基因组中分离得到2.4 kb的麦芽寡糖基  相似文献