奶牛抗热应激中草药添加剂对小鼠热耐力的影响     

孙齐英 《畜禽业》2008,(10)

试验研究奶牛抗热应激中草药添加剂对热应激小鼠机体耐力的影响。试验分A、B、C、D4个处理,每个处理20只小鼠,分别喂以不同添加剂浓度的饲料21d[A组:0.5g/(10g体重)、B组:0.25g/(10g体重)、C组:0.13g/(10g体重)、D组0g/(10g体重)],每日光暗节律各12h,自由摄食与饮水。试验结果表明,A、B组体重增加显著高于C、D组(P<0.01),A、B、C组小鼠死亡数显著少于D组(P<0.05),受热90min后,A、B、C组小鼠肛温比D组分别低0.3℃、0.3℃、0.5℃(P<0.05),说明该添加剂具有一定的提高小鼠热耐力作用。  相似文献   

Extension of shelf life of tuberose flowers using a combination of gamma irradiation and generally regarded as safe (GRAS) preservatives and assessment of antimicrobial potency of senesced flowers     

Probir Kumar Ghosh  Satadal Das 《The Journal of Horticultural Science and Biotechnology》2017,92(2):130-145

Tuberose cut flowers, available as loose flowers, were treated with gamma (γ) irradiation and generally regarded as safe (GRAS) preservative solutions for extension of shelf life. The flowers were packaged in low-density polyethylene bags, heat sealed and stored at 23 ± 2°C, 80% relative humidity (RH) and 4 ± 1°C, 40% RH, respectively. The flowers stored at these two temperature regimes were subjected to sensory evaluation and biochemical analyses. From these assessments, the longest shelf life of tuberose flowers was found to be 8 days at 23 ± 2°C, 80% RH (compared to 4 days for control) and 24 days at 4 ± 1°C, 40% RH (compared to 8 days for control) using combination treatment of low dose γ-irradiation (0.02 kGy) and preservative solutions (4% sucrose and 0.02% CaCl₂). Ethanolic extract of tuberose flowers of the most shelf stable set (stored at 4 ± 1°C), i.e. at the end of 24 days, showed antimicrobial potency against the common skin pathogen Staphylococcus aureus (ATCC 25923 and MDR strains), suggesting utility of the senesced tuberose flowers for therapeutic applications. This preservation technique would promote export of tuberose flowers by extension of their shelf lives and allow utilization of these flowers, post senescence.  相似文献   

Detection of challenge virus in fetal tissues by nested PCR as a test of the potency of a porcine parvovirus vaccine   总被引：1，自引：0，他引：1  

Belák  S.  Rivera  E.  Ballagi-Pordány  A.  Hanzhong  W.  Widén  F.  Soós  T. 《Veterinary research communications》1998,22(2):139-146

To estimate the potency of a porcine parvovirus (PPV) vaccine, three vaccinated and three non-vaccinated pregnant gilts were infected with PPV and the distribution of the virus was studied in the tissues of their 51 fetuses. Virus detection was attempted using haemagglutination (HA) and immunofluorescence (IF) assays, as well as by standard (single) and nested polymerase chain reactions (PCR). None of the detection methods yielded positive results when used to test for the presence of virus in suspensions of organs from the fetuses from the vaccinated gilts. However, the virus was detected in the fetuses from non-vaccinated gilts as follows: HA was positive in 14 cases out of 23 (60.8%), IF in 16/23 (69.5%), standard PCR in 12/20 (60%), and the nested PCR in 19/23 (82.6%). Although the correlation among the results of various methods of virus detection was rather close (r<0.83), the sensitivity of the nested PCR was the highest, both when testing dilutions of PPV and when analysing the fetal organs. The nested PCR therefore provides a reliable approach for studies of virus distribution in fetal organs, with special reference to potency tests on vaccines.  相似文献   

鸡毒支原体灭活疫苗效力检验气囊注射攻毒方法的研究     

魏津  刘博  马欣  王甲  赵浩然  刘元杰  冯妍  刘业兵  罗玉峰 《中国兽药杂志》2022,56(10):12-17

鸡毒支原体灭活疫苗效力检验采用的喷雾攻毒方法所需菌液量大,结果易受喷雾时间和器械设备影响。为了探索一种简便、易行且结果稳定的新方法,本研究冻干了一批攻毒用菌株鸡毒支原体R株,对其性状、纯粹、真空度、剩余水分、培养特性、特异性、活菌计数和均一性等进行检定,结果全部符合规定。将其稀释成不同活菌数,采用气囊注射方式攻毒,观察试验结果并与《中国兽药典(三部)》中的喷雾方法进行比较。结果表明,建立的气囊注射攻毒法稳定、准确,通过5批次疫苗效力检验试验比较,与喷雾方法结果无显著差异,可为该类疫苗攻毒方法优化提供新思路。  相似文献   

平潭海岛防护林的减灾稳产效益     

卢成林  陈昌茂 《福建林学院学报》1992,12(1):121-124

本文报导了平潭县营造海岛防护林、经济林的成功经验,详述了海岛防护林在防风固沙、经受台风袭击中所发挥的防护作用及其所带来的经济效益。  相似文献   

提高苏云金杆菌对棉铃虫毒力的研究   总被引：5，自引：2，他引：3  

尹新明  郭线茹  贾新成  吴云汉  邱立友 《华北农学报》1999,14(2):67-72

在含有Ｂｔ毒素的人工饲料中,通过加入碱性化合物提高其对昆虫中肠的作用,或加入蛋白酶激活剂、无机化合物和有机来提高Ｂｔ的毒力效价。试验结果表明,加入碳酸钙、硫酸锌、氯化镁、苹果酸、马来酸、甲酸和巯基乙醇酸钠,可使Ｂｔ毒力效价分别提高０．９５１．２２,０．３７,０．４７,１．１７,２．１０和９．０倍,其中加入硫酸锌、甲酸和巯基乙醇酸钠的增效效果较好。  相似文献   

Comparison of adherence tendencies of pesticide residues sprayed on small-, medium-, and large-sized tomatoes     

Takuya Nagata  Hikari Dobashi  Kazuaki Iijima  Kazutoshi Ohyama 《Journal of Pesticide Science》2021,46(4):352

Crop field trials were conducted to investigate the residues of sprayed pesticides on the different sizes of tomatoes. Pesticide residue data in tomatoes varied due to different locations of the three crop fields selected and/or physicochemical properties of the three pesticides tested. The pesticide residue levels in the medium- and small-sized tomatoes were 1.5 and 2.4 times higher than the level in large-sized tomatoes under similar spray conditions, whereas amount of pesticides adhered per unit surface area were approximately equal among all three sizes of tomatoes. The results of this study suggested that the differences in pesticide residue levels were due to differences in the degree of specific surface area of each tomato size. Resultant residue data of medium-sized tomatoes demonstrated a proportional relationship between pesticide residue levels and the specific surface area of tomatoes.  相似文献   

二甲基亚砜溶剂用于制霉菌素效价测定     

于海兵  谢卫国  褚志刚  阮琼芳  李华文  李敏  丁元林  孔丹莉  姚大纯 《湛江医学院学报》2017,35(3)

目的 探讨二甲基亚砜(DMSO)代替N,N-二甲基甲酰胺(DMF)用于制霉菌素效价测定的可行性.方法 制霉菌素分别用DMSO和DMF溶解,采用美国药典中抗生素-微生物测定法检测制霉菌素效价.结果 DMF和DMSO均有较好的准确性、精密度、特异性、线性范围(10.0-40.0 U/mL)、检出限(2.0 U/mL)和稳定性,且DMSO方法的灵敏度、定量下限(LOQ,3.0 U/mL)优于DMF.结论 鉴于DMSO毒性明显低于DMF,且DMSO方法的分析性能特征与DMF法相当甚或更好,故DMSO可代替DMF作为溶剂用于制霉菌素活性测定.  相似文献   

不同栽培管理措施对云南古茶树树势恢复的研究   总被引：3，自引：0，他引：3  

殷丽琼  刘德和  王平盛  JIANG Hui-bing  YI Bing  YU Xiang-shuai 《西南农业学报》2010,23(2)

云南省是中国古茶树分布最广、面积最大的省份,但由于对古茶园栽培管理不当,造成很多古茶树衰退。本研究采用科学的栽培管理技术对云南保塘古茶树的树冠、树幅、叶层厚度、新梢生长、鲜叶产量进行分析。结果表明,耕作-施肥-合理留叶采摘春、秋茶-留养夏梢的措施能明显使茶树生长势增强,产量提高。这对加强古茶树的保护及茶叶生产具有重要的意义。  相似文献   

和田地区绿洲外围农田防护林带的防护效益   总被引：3，自引：1，他引：2  

王翠  雷加强  李生宇  毛东雷  再努拉&#;热合木吐拉  周杰 《水土保持通报》2014,(1):98-103,122

在和田地区新垦农地外围的流动沙地种植新疆杨乔木+沙拐枣灌木复合防护林,通过布设风速风向观测仪、沙尘通量梯度仪、温湿度和辐射等测定仪于林带前后,研究防护林网的防护效益,为营建绿洲外围防护林和合理开发利用荒漠区的土地资源提供理论依据。结果表明,防护林带的防风效能随着高度的增加而减弱,大于起沙风速(6m/s)时,新疆杨林带、沙拐枣林带在0.5m处的防风效能分别为67.2%和94.5%,在3m处的防风效能分别为31.3%和33.7%。随着风速的增大,沙拐枣林带和新疆杨林带的防风效能均减弱,新疆杨林带的减弱更为明显。防护林带有效地降低了地表风速,减轻地表风蚀。林带内的输沙量仅为外围的10%左右。此外,林带内的太阳辐射强度下降,温度降低,湿度显著增加。由此可见,防护林可以有效地起到防风固沙,改善区域小气候的作用。  相似文献